Remembering God's goodness, faithfulness, and love, his good will and power over sin, death and the devil, and having a view that sees beyond the immediate next moments are the building blocks of patience. And this is the realm of the Holy Spirit's work. The Holy Spirit helps us become more aware of these things as he focuses our attention on Jesus. Our preoccupation with the things of this world will lead us to works of impatience, frustration, and anxiety.
My mind is definitely not focused on the spiritual realm and the promises of God when I'm frustrated behind the slow driver. But a focus on God's promises, Jesus' death and resurrection, coupled with a humble spirit will ground me in an attitude of patience that gives testimony to the Holy Spirit's work in me.