In this episode of Exploration Science, Drs. Ronald Frank (AIMS Scientific Products GmbH) and
Christian Behn (CEM) discuss the evolution of SPOT Synthesis to generate
peptide libraries. They also correct some misinformation and bring to light the
ease and utility of applying SPOT arrays to biological studies.
Watch on YouTube: https://youtu.be/MBJFIygXDRg
Correction: The founding of AIMS company was in 1998, not 1989.
See the links and references below for some examples of SPOT applications:
AIMS Scientific Products http://www.aims-scientific-products.de/index.php
CEM MultiPep: https://cem.com/multipep2
Clemens Schulte, Vladimir Khayenko, Amit Jean Gupta, Hans Michael Maric. Low-cost
synthesis of peptide libraries and their use for binding studies via
temperature-related intensity change. STAR Protocols. Volume 2, Issue 3,
2021, 100605. https://doi.org/10.1016/j.xpro.2021.100605.
Melder FTI, Lindemann P, Welle A, Trouillet V, Heißler S, Nazaré M, Selbach M. Compound
Interaction Screen on a Photoactivatable Cellulose Membrane (CISCM) Identifies
Drug Targets. ChemMedChem. 2022 Oct 6;17(19):e202200346. doi:
10.1002/cmdc.202200346. Epub 2022 Sep 20. PMID: 35867055; PMCID: PMC9826412.
Mohammed El-Mowafy, Ursula Bilitewski. Identification of possible Ser/Thr/Tyr
phosphorylation sites in the fungal histidine kinase CaNik1p by peptide array
technique. Bulletin of Faculty of Pharmacy, Cairo University. Volume 56,
Issue 1, 2018, Pages 68-72. https://doi.org/10.1016/j.bfopcu.2018.03.007.
Moreno-Yruela, C., Bæk, M., Vrsanova, AE. et al. Hydroxamic acid-modified peptide
microarrays for profiling isozyme-selective interactions and inhibition of
histone deacetylases. Nat Commun 12, 62 (2021). https://doi.org/10.1038/s41467-020-20250-9.
Meyer et al., Mutations in Disordered Regions Can Cause Disease by Creating Dileucine
Motifs. Cell (2018), https://doi.org/10.1016/j.cell.2018.08.019.
Rebollo, A., Fliedel, L., Tuffery, P., PEPscan: A Broad-Spectrum Approach for the
Characterization of Protein-Binder Interactions? Biomolecules 2022, 12,
178. https://doi.org/10.3390/
Rosie M. Jarrald, Aw W. Liang Alvin, Andrea E. Rawlings, Masayoshi Tanaka, Mina Okochi,
and Sarah S. Staniland. Systematic Screening and Deep Analysis of CoPt
Binding Peptides Leads to Enhanced CoPt Nanoparticles Using Designed Peptides. Bioconjugate
Chemistry 2020 31 (8), 1981-1994. DOI: 10.1021/acs.bioconjchem.0c00348.
