In this episode of the Epigenetics Podcast, we caught up with Jane Skok from New York University School of Medicine to talk about her work on spatio-temporal alterations in chromosome dynamics.
Studies demonstrating that nuclear organization and long-range chromatin interactions play essential roles in gene regulation have been the focus of the Skok Lab, where the team has played a leading role. Their initial studies focused on lymphocyte development and the control of V(D)J recombination, a key part of generating the diverse repertoire of B-cell antibodies and T-cell receptors. The Skok Lab was among the first to demonstrate the possibility of chromatin forming dynamic loops which lead to the formation of reversible intra-locus loops in the immunoglobulin and T-cell receptor loci and to a profound impact on the ability of B and T cells to generate receptor diversity.
References
Roldán, E., Fuxa, M., Chong, W., Martinez, D., Novatchkova, M., Busslinger, M., & Skok, J. A. (2005). Locus “decontraction” and centromeric recruitment contribute to allelic exclusion of the immunoglobulin heavy-chain gene. Nature Immunology, 6(1), 31–41. https://doi.org/10.1038/ni1150
Skok, J. A. (2014). Taking a break from the lab: Can it really be done? Trends in Cell Biology, 24(12), 725–726. https://doi.org/10.1016/j.tcb.2014.09.002
Proudhon, C., Snetkova, V., Raviram, R., Lobry, C., Badri, S., Jiang, T., Hao, B., Trimarchi, T., Kluger, Y., Aifantis, I., Bonneau, R., & Skok, J. A. (2016). Active and Inactive Enhancers Cooperate to Exert Localized and Long-Range Control of Gene Regulation. Cell Reports, 15(10), 2159–2169. https://doi.org/10.1016/j.celrep.2016.04.087
Lhoumaud, P., Sethia, G., Izzo, F., Sakellaropoulos, T., Snetkova, V., Vidal, S., Badri, S., Cornwell, M., Di Giammartino, D. C., Kim, K.-T., Apostolou, E., Stadtfeld, M., Landau, D. A., & Skok, J. (2019). EpiMethylTag: Simultaneous detection of ATAC-seq or ChIP-seq signals with DNA methylation. Genome Biology, 20(1), 248. https://doi.org/10.1186/s13059-019-1853-6
Nishana, M., Ha, C., Rodriguez-Hernaez, J., Ranjbaran, A., Chio, E., Nora, E. P., Badri, S. B., Kloetgen, A., Bruneau, B. G., Tsirigos, A., & Skok, J. A. (2020). Defining the relative and combined contribution of CTCF and CTCFL to genomic regulation. Genome Biology, 21(1), 108. https://doi.org/10.1186/s13059-020-02024-0
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