This article introduces a Stereotaxic Topographic Atlas of the Mouse Brain (STAM), a groundbreaking three-dimensional (3D) reference atlas with an isotropic 1-micrometer resolution. Developed using continuous micro-optical sectioning tomography and Nissl-based cytoarchitecture, it provides highly detailed 3D topographies of 916 brain structures, enabling single-cell level spatial localization. The atlas includes an informatics platform for visualization, sharing, neuronal circuit mapping, and intelligent stereotaxic surgery planning. This novel tool addresses limitations of traditional atlases by offering arbitrary-angle slice generation and interoperability with existing atlases, significantly advancing large-scale brain mapping research.
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