Researchers have developed a suite of new protocols, collectively called FX-Cell, to improve single-cell RNA sequencing in diverse plant species. These methods utilize coagulant fixatives to stabilize cells, allowing them to withstand the high temperatures and mechanical forces needed to break down rigid cell walls. A critical component of the workflow involves a column-based purification step that removes harmful RNases from digestive enzymes, thereby preserving the integrity of the genetic data. This approach enables the study of cryopreserved field samples and reveals accurate wounding responses that traditional methods often distort. By overcoming the technical limitations of standard protoplast isolation, these advancements facilitate the creation of comprehensive Plant Cell Atlases for both model and non-model organisms. Consistent with its versatility, the method successfully captured complex developmental data in tissues as varied as maize anthers and wild rice rhizomes.
References:
- Ming X, Wan M C, Zhang Z D, et al. FX-Cell: a method for single-cell RNA sequencing on difficult-to-digest and cryopreserved plant samples[J]. Nature Methods, 2025: 1-12.
