In episode 28 of “The Conversational Flow”, our hosts, Adam and Brian, discuss why standardization is essential for credible flow cytometry data at scale. They walk through the biggest sources of variability - reagents and lots, sample handling and shipping time, anticoagulants and processing choices (whole blood vs. PBMCs, fresh vs. cryopreserved), instrument setup, and downstream analysis - and explain how each one can create “noise” that masquerades as biology. They note practical realities like weather delays and circadian effects, and why stability studies help define which markers remain trustworthy over time. The conversation also highlights where the field has evolved since 2012: automated gating is now effectively required as panels grow, and tools like dried panels and standardized nomenclature can reduce batch-to-batch variability and improve cross-study aggregation.
“The Conversational Flow” an official podcast of KCAS Bio, and focuses on the more niche topic of Flow Cytometry and its role within the greater pharmaceutical, biopharmaceutical and bioanalytical industries. Every month, KCAS Bio will bring you another 60 minutes (or so) of friendly banter between our two finest Flow Cytometry experts as they chat over coffee and discuss what they’ve learned about the world of Flow Cytometry the past couple of weeks. “The Conversational Flow” is brought to you by KCAS Bio.
KCAS Bio is a progressive growing contract research organization of well over 250 talented and dedicated individuals with growing operations in Kansas City, Doylestown, PA, and Lyon, France, where we are committed to serving our clients and improving health worldwide. Our experienced scientists provide stand-alone bioanalytical services to the pharmaceutical, biopharmaceutical, animal health and medical device industries.
