A comprehensive guide for the laboratory study of malignancy. It provides rigorous instructions for maintaining cancer cell lines, with specific emphasis on preventing genetic drift and cross-contamination. The text outlines diverse research methodologies, ranging from 3D co-culture systems and murine xenograft models to advanced molecular techniques like CRISPR-Cas9 gene editing. Researchers can also find detailed workflows for analyzing cellular components through mass spectrometry, flow cytometry, and RNA sequencing. Additionally, the volume addresses critical logistical needs, such as the cryopreservation of tissues and the biobanking of patient samples. Ultimately, these sources provide the technical foundation necessary to investigate tumor biology and evaluate potential medical treatments.
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