Shows
The Single-Cell World PodcastEp. 60: Edge Precison Manufacturing: The Hidden Makers of Single-Cell and Spatial Plastic ConsumablesIn today’s episode, the final episode of The Single-Cell World, Cátia makes a surprising confession. Before speaking with today’s guest, she always assumed that the plastic consumables in single-cell and spatial transcriptomics kits were made by the same companies that developed and commercialized the technology.But, as it turns out, that’s not the case. In fact, many of these critical consumables are produced by specialized manufacturers—like Edge Precision Manufacturing.To know more about how these essential components are designed and created, Cátia talks with Andrew Kamholz, the CEO of Edge Preci...2025-01-211h 07
The Single-Cell World PodcastEp. 59: Rethink What You Know About Chromium Single Cell10x Genomics is world-class for single cell sequencing technology. As the company continues to innovate and launch exciting new products, it can be challenging to stay up to date on the portfolio and technology, even for us experts. In this episode, I talk to Michael Schnall-Levin, Chief Technology Officer and Founding Scientist at 10x Genomics. He shares everything you need to know about their Chromium single cell sequencing solutions. We cover Chromium instruments, new GEM-X assays, pricing updates, and more.Tune in now and get a clear idea of all the types of experiments that you can...2024-12-1246 min
The Single-Cell World PodcastEp. 58: TileDB-SOMA - Scalable Cloud Storage for Single-CellTileDB software is transforming how scientists manage and analyze data. This includes single-cell omics data. In this episode, Tommy Tang and I talk to Aaron Wolen, Principal Software Engineer at TileDB, to explore the unique capabilities of TileDB-SOMA. Aaron breaks down how this specific software addresses the complexities of single-cell sequencing data, offering robust data management, streamlined workflows, and scalable analysis tools tailored to any needs. We discuss its core features, real-world applications, and the transformative impact it has on accelerating scientific discovery in single-cell genomics research.Tune in now to learn how TileDB-SOMA can help streamline...2024-12-0533 min
The Single-Cell World PodcastEp. 57: EVOBYTE: Simple Solutions for Complex Biomedical DataHosted by: Catia Moutinho & Tommy Tang Guest: Jonathan Alles, Founder of EVOBYTE Digital BiologyIn this episode, Jonathan tells us how EVOBYTE’s EVO Data platform is revolutionizing data management for research labs, with a focus on single-cell research. Our conversation covers EVOBYTE’s unique solutions, customization capabilities, practical applications, and the role of automation in bioinformatics workflows. Jonathan also shares his insights on the future of computational biology and advice for budding bioinformaticians.Links that may interest you:* Introducing EvoData Platform* ReadStore is a platform for storing, managing, and i...2024-11-1451 min
The Single-Cell World PodcastEp. 56: scGET-seq. Studying chromatin at higher resolutionIn this podcast episode, I talk with Francesca Giannese about an exciting new method for studying chromatin at the single-cell level called scGET-seq. Unlike previous methods such as scATAC-seq, which only allow us to study open chromatin, scGET-seq provides insights into both open and closed chromatin. This breakthrough can lead to a deeper understanding of how chromatin structure impacts gene regulation in individual cells. Tune in to learn more about how scGET-seq works and its potential applications in research.Contacts for support on scGET-seq implementation:* Francesca Giannese (wet-lab protocol implementation - enzyme requests)...2024-10-1738 min
The Single-Cell World PodcastEp. 55: Unlocking Single-Cell Secrets with Catia MoutinhoThis episode features the audio from an interview where Divya Dhasman from GenoWrite speaks with Catia Moutinho. They discuss the evolution of The Single-Cell World and explore the role of technology in advancing genomics. Special thanks to Paul Naphtali for making this interview possible. Catch the full interview here.Thanks to you for listen. Hit the ❤️ if you liked it and share it with your colleagues. This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2024-09-1216 min
The Single-Cell World PodcastEp. 54: SHASTA KITS - New single-cell solutions from Takara BiosciencesIn this episode, I speak with Shuwen Chen, a product manager at Takara Bio, who provides an in-depth look at the various solutions they offer for single-cell research projects. We discuss the Smart-Seq kits, explore their single-cell platforms, and dive into the latest Shasta kits for RNA and DNA. Tune in to find out if these innovative solutions can enhance your research projects.Useful links* Arc-well: a new method for single-cell DNA-seq analysis of formalin-fixed paraffin-embedded samples* CUT&Tag for efficient epigenomic profiling of small samples and single cells* Support this...2024-08-1537 min
The Single-Cell World PodcastEp. 54: SHASTA KITS - New single-cell solutions from Takara BiosciencesIn this episode, I speak with Shuwen Chen, a product manager at Takara Bio, who provides an in-depth look at the various solutions they offer for single-cell research projects. We discuss the Smart-Seq kits, explore their single-cell platforms, and dive into the latest Shasta kits for RNA and DNA. Tune in to find out if these innovative solutions can enhance your research projects. Useful linksArc-well: a new method for single-cell DNA-seq analysis of formalin-fixed paraffin-embedded samplesCUT&Tag for efficient epigenomic profiling of small samples and single cellsS...2024-08-1537 min
The Single-Cell World PodcastEp. 53: CYTENA: Automations for Single-Cell ExperimentsIn this episode, I talk with Adrian Zambrano, Customer Success Manager at CYTENA. We’ll discuss instruments that can be used in single-cell projects, including upstream tools like single cell dispensation instruments and downstream solutions like automatic bead clean-up.Discover innovative solutions from CYTENA that could enhance your research projects. Tune in to learn how these tools can make our lab work easier and more efficient.Useful links* Resources Cytena* Support this podcast. 👉🏻 Pay us a Ko-fi This is a public episode. If you would like to discu...2024-08-0139 min
The Single-Cell World PodcastEp.53: CYTENA: Automations for Single-Cell ExperimentsIn this episode, I talk with Adrian Zambrano, Customer Success Manager at CYTENA. We’ll discuss instruments that can be used in single-cell projects, including upstream tools like single cell dispensation instruments and downstream solutions like automatic bead clean-up. Discover innovative solutions from CYTENA that could enhance your research projects. Tune in to learn how these tools can make our lab work easier and more efficient. Useful linksResources CytenaSupport this podcast. 👉🏻 Pay us a Ko-fi Get full access to Single-Cel...2024-08-0139 min
The Single-Cell World PodcastEp. 52: BD Biosciences-High-throughput, Multiomics and ATAC-seq Single-Cell SolutionsBD Biosciences, known for their cytometers, also offers impressive single-cell sequencing solutions. Today’s guest is Hasi Patel, Senior Single Cell Solutions Architect, who tells me everything about this technology during this episode.Hasi starts explaining how BD's latest high-throughput single-cell platform works, as well as all the multiomics approaches that can be done with their innovative solutions. In all of them, sample multiplexing is possible, enhancing its versatility. Hasi also shares valuable tips on planning single-cell sequencing experiments. Tune in now to learn more about BD Biosciences' innovations in single-cell sequencing!Useful Lin...2024-07-1849 min
The Single-Cell World PodcastEp. 52: BD Biosciences- High-throughput, Multiomics and ATAC-seq Single-Cell SolutionsBD Biosciences, known for their cytometers, also offers impressive single-cell sequencing solutions. Today’s guest is Hasi Patel, Senior Single Cell Solutions Architect, who tells me everything about this technology during this episode. Hasi starts explaining how BD's latest high-throughput single-cell platform works, as well as all the multiomics approaches that can be done with their innovative solutions. In all of them, sample multiplexing is possible, enhancing its versatility. Hasi also shares valuable tips on planning single-cell sequencing experiments. Tune in now to learn more about BD Biosc...2024-07-1849 min
The Single-Cell World PodcastEp51. A4cell Lab-in-A-Cell Devices:In this episode, Irene González, the International Key Account Manager at Arrays For Cell Nanodevices, SL (A4cell), discusses their microdevices known as SPAchips. She explains how these devices allow us to directly measure various characteristics in living cells, such as reactive oxygen species (ROS), calcium and pH levels.A4Cell email: info@a4cell.comSupport our podcast: pay us a Ko-fi This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2024-06-2724 min
The Single-Cell World PodcastEp51. A4cell Lab-in-A-Cell Devices: Eyes inside single cellsIn this episode, Irene González, the International Key Account Manager at Arrays For Cell Nanodevices, SL (A4cell), discusses their microdevices known as SPAchips. She explains how these devices allow us to directly measure various characteristics in living cells, such as reactive oxygen species (ROS), calcium and pH levels. A4Cell email: info@a4cell.comSupport our podcast: pay us a Ko-fi Get full access to Single-Cell World's Substack at thesinglecellworld.substack.com/subscribe2024-06-2724 min
The Single-Cell World PodcastSpatial Transcriptomics Legal BattlesJoin me, Cátia Moutinho, as I break down the basics of spatial transcriptomics legal battles ⚖️ between companies in this field. I'll share my opinions 💭, propose solutions 🛠️, and raise open questions ❓ that might have you thinking. This is a message for judges, companies teams and researchers. 😉Tune in now! 🎧The Single-Cell World Blog, Newsletter.Pay us a Kofi This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2024-06-1329 min
The Single-Cell World PodcastEp.50: Spatial Transcriptomics Legal Battles: The Voice of a ResearcherJoin me, Cátia Moutinho, as I break down the basics of spatial transcriptomics legal battles ⚖️ between companies in this field. I'll share my opinions 💭, propose solutions 🛠️, and raise open questions ❓ that might have you thinking. This is a message for judges, companies teams and researchers. 😉Tune in now! 🎧 The Single-Cell World Blog, Newsletter.Pay us a Kofi Get full access to Single-Cell World's Substack at thesinglecellworld.substack.com/subscribe2024-06-1329 min
The Single-Cell World PodcastEp.49: Singleron Technology: 4 Kits for Single-Cell Multi-OmicsIn this episode, Ritika Kulshreshtha, Singleron Account Manager, tells me everything about their different kits for single cell experiments. For example, a kit to study DNA and RNA in parallel, another to study glycosylation, mRNA from yeast, or nascent RNA. So, if you are interested in doing novel single cell experiments, listen to this episode now!Useful links:Singleron Boot CampSupport our Podcast (2$ per month) This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack...2024-05-2343 min
The Single-Cell World PodcastEp.49: Singleron Technology- 4 Kits for Single-Cell MultiomicsIn this episode Ritika Kulshreshtha, Singleron Account Manager tells me everything about their different kits for single cell experiments. For example, a kit to study DNA and RNA in parallel, another to study glycosylation, mRNA from yeast, or nascent RNA. So, if you are interested in doing novel single cell experiments, listen to this episode now! Useful links:Singleron Boot CampSupport our Podcast (2$ per month) Get full access to Single-Cell World's Substack at thesinglecellworld.substack.com/subscribe2024-05-2343 min
The Single-Cell World PodcastEp. 48. VASA-seq: Coding and Non-coding RNAHi there 👋 The topic of this episode is VASA-seq, a single-cell transcriptomics method that we can use to study coding and non-coding RNA. Listen now to my conversation with Fredrik Salmén, the first author of the paper where VASA-seq is described. He gives me an amazing explanation about this method, besides his opinion on other topics related to single-cell and spatial experiments. You will not want to skip this one!Other useful links:* Pay us a Ko-fi* SORT-seq* Atrandi Biosciences* Single Cell Discoveries* Genevia Technologies2024-05-0938 min
The Single-Cell World PodcastEp. 48. VASA-seq: Coding and Non-coding RNAHi there 👋 The topic of this episode is VASA-seq, a single-cell transcriptomics method that we can use to study coding and non-coding RNA. Listen now to my conversation with Fredrik Salmén, the first author of the paper where VASA-seq is described. He gives me an amazing explanation about this method, besides his opinion on other topics related to single-cell and spatial experiments. You will not want to skip this one!Other useful links:Pay us a Ko-fiSORT-seqAtrandi BiosciencesSingle Cell DiscoveriesGenevia Technologies Get f...2024-05-0938 min
The Single-Cell World PodcastEp. 47: Levitation Technology - A Gentle Approach to Viable Cells and Nuclei IsolationIn this podcast episode, I have the pleasure of talking with Kevin Travers, the Senior Vice President for R&D at LevitasBio.Kevin tells me everything about their gentle and label-free technology that can be used during sample preparation for any single cell sequencing experiments, but also for other downstream applications.🎧Tune in now and learn how levitation technology can make your experiments better.✨Useful Links:* Levitation Technology for Single Cell Genomics* The LeviCell Systems for Cell Isolation and Enrichment This is a pub...2024-04-2542 min
The Single-Cell World PodcastEp. 47: Levitation Technology- A Gentle Approach to Viable Cells and Nuclei IsolationIn this podcast episode, I have the pleasure of talking with Kevin Travers, the Senior Vice President for R&D at LevitasBio. Kevin tells me everything about their gentle and label-free technology that can be used during sample preparation for any single cell sequencing experiments, but also for other downstream applications. 🎧Tune in now and learn how levitation technology can make your experiments better.✨ Useful Links:Levitation Technology for Single Cell GenomicsThe LeviCell Systems for Cell Isolation and Enrichment Support our po...2024-04-2542 min
The Single-Cell World PodcastEp.46: Piccolo- A New Normalization Method for scRNA-seqJoin us on this podcast episode as I explore the fascinating world of single-cell RNA sequencing (scRNA-seq) data normalization with Amartya Singh, a post-doctoral researcher at Reuters. Discover the limitations of the standard methods used in scRNA-seq data normalization and how Amartya's innovative solution is changing the game. Hear firsthand about his journey of inspiration and the ideas that led to the development of this groundbreaking approach. Don't miss out on this insightful discussion - tune in now!Useful Links:* Amartya Singh LinkedIn and GitHub profile* The starting point publication * TuBA p...2024-04-1103 min
The Single-Cell World PodcastEp.46: Piccolo- A New Normalization Method for scRNA-seqJoin us on this podcast episode as I explore the fascinating world of single-cell RNA sequencing (scRNA-seq) data normalization with Amartya Singh, a post-doctoral researcher at Reuters. Discover the limitations of the standard methods used in scRNA-seq data normalization and how Amartya's innovative solution is changing the game. Hear firsthand about his journey of inspiration and the ideas that led to the development of this groundbreaking approach. Don't miss out on this insightful discussion - tune in now!Useful Links:Amartya Singh LinkedIn and GitHub profileThe starting point publication TuBA paper2024-04-1149 min
The Single-Cell World PodcastEp 45: High Resolution Pathology: Molecular Cartography from Resolve BiosciencesIn this insightful podcast interview with Kieren Patel, the Chief Business Officer at Resolve Biosciences, we delve into the world of Molecular Cartography technology. Kieren provides details about this innovative platform, underscoring its remarkable capabilities in facilitating high molecular resolution pathology. Additionally, Kieren sheds light on forthcoming solutions set to be commercialized later this year, with a particular emphasis on their commitment to multiomics and customized approaches. Tune in to gain valuable insights into the latest advancements in molecular cartography and discover the promising future that Resolve Biosciences' offerings hold.Useful links:Resolve Biosciences 2024-03-2833 min
The Single-Cell World PodcastEp 45: High Resolution Pathology: Molecular Cartography from Resolve BiosciencesIn this insightful podcast interview with Kieren Patel, the Chief Business Officer at Resolve Biosciences, we delve into the world of Molecular Cartography technology. Kieren provides details about this innovative platform, underscoring its remarkable capabilities in facilitating high molecular resolution pathology. Additionally, Kieren sheds light on forthcoming solutions set to be commercialized later this year, with a particular emphasis on their commitment to multi-omics and customized approaches. Tune in to gain valuable insights into the latest advancements in molecular cartography and discover the promising future that Resolve Biosciences' offerings hold. Useful links:2024-03-2833 min
The Single-Cell World PodcastEp. 44: Scale Biosciences Innovating scDNA methylation and scRNAseqIn this podcast episode, I had the privilege of talking with Melanie Masuda, the Director of Market Development at Scale Biosciences. Melanie told me all about Scale Biosciences' cutting-edge technology and gave me a full overview of all of their different kits.Listen now and understand how the single-cell (sc) sequencing DNA methylation, the scRNAseq, and the CRISPR enrichment kits work. Maybe this is the solution for your research project!Useful Links:Melanie Masuda LinkedInScale Biosciences(website)* Twitter* LinkedInKITS* scDNA methylation2024-03-1433 min
The Single-Cell World PodcastEp. 44: Scale Biosciences Innovating scDNA methylation and scRNAseqIn this podcast episode, I had the privilege of talking with Melanie Masuda, the Director of Market Development at Scale Biosciences. Melanie told me all about Scale Biosciences' cutting-edge technology and gave me a full overview of all of their different kits. Listen now and understand how the single-cell (sc) sequencing DNA methylation, the scRNAseq, and the CRISPR enrichment kits work. Maybe this is the solution for your research project! Useful Links:Melanie Masuda LinkedInScale Biosciences(website)Twitter2024-03-1433 min
The Single-Cell World PodcastEp.43: The SMART-seq Family-full length RNA methodsIn this insightful podcast episode, I have the privilege of interviewing Simone Picelli, the Head of the Single-Cell Genomics Platform at the Institute of Ophthalmology Basel. As we talked, we got into the specifics of single-cell RNA sequencing (scRNA-seq) full-length methods from the SMART-seq family.Simone shared valuable insights into various methods within, including SMART-seq, SMART-seq 2, SMART-seq 3, FLASH-seq, FLASH-seq low-amplification, and FLASH-seq with UMI. We explored the unique features and applications of each method, highlighting their contributions to advancing single-cell genomics research. In addition, we also talked about other common methods and the bigger picture o...2024-02-2959 min
The Single-Cell World PodcastEp.43: The SMART-seq Family-full length RNA methodsIn this insightful podcast episode, I have the privilege of interviewing Simone Picelli, the Head of the Single-Cell Genomics Platform at the Institute of Ophthalmology Basel. As we talked, we got into the specifics of single-cell RNA sequencing (scRNA-seq) full-length methods from the SMART-seq family.Simone shared valuable insights into various methods within, including SMART-seq, SMART-seq 2, SMART-seq 3, FLASH-seq, FLASH-seq low-amplification, and FLASH-seq with UMI. We explored the unique features and applications of each method, highlighting their contributions to advancing single-cell genomics research. In addition, we also talked about other common methods and the b...2024-02-2959 min
The Single-Cell World PodcastEp. 42: Updating Episode 14, Single-Cell Methods-an overviewThe Single-Cell World Podcast is now available in video as well, but just on Apple Podcast and in our new YouTube channel 😉Episode 14: Single-Cell Methods, an overview, needed an update since there is a fourth method that is being used to individualize cells when using single-cell sequencing technology.Join me and listen to it, as well as other updates concerning the methods that are at the core of the single-cell sequencing technology.Here I leave you a gift, related with this episode.Thank you for listening.Kind Regards,...2024-01-1107 min
The Single-Cell World PodcastEp.41: Single-Cell Sequencing Products 2023 Review_Part 2Hi there!Today is Part 2 of the review of single-cell-related products launched in 2023. I hope I don't miss any. If I do, send me a message telling me about it. 😉Here are some useful links:Exploring Single-Cell Sequencing online courseCompanies that I talk during this episode (part 2):Scale BiosciencesScipio BioscienceSingleron BiotechnologiesTakara Bio10X GenomicsFree ResourcesSponsorshipPay me a Ko-FiThanks for listening!Cheers,2023-12-1113 min
The Single-Cell World PodcastEp. 40: Single-Cell Sequencing Products Review 2023_Part1Hi there!Today I review single-cell-related products launched in 2023. They are a lot! I hope I don't miss any. If I do, send me a message telling me about it. 😉Here are some useful links:Join the Single-Cell World NewsletterCompanies that I talk during this episode (part 1):BD Biosciences Ep. 18: Samples MultiplexingBioScryb GenomicsFluent BioSciencesEpisode 31: Podcast Fluent BioSciencesHoneycomb BiotechnologiesEpisode 19: Podcast Honeycomb BiotechnologiesMission BioEpisode 16: Podcast Miss...2023-12-0418 min
The Single-Cell World PodcastEp. 39: My Contribution to Frontline Genomics Spatial & Single-Cell Analysis PlaybookHello there!The Spatial and Single-Cell Analysis Playbook was released last week. Front Line Genomics produced it. I was honored to be invited to take part in it.In this episode, you can hear the questions that were asked (AI voice here) and my responses during this fantastic interview.I hope you like it!Useful LinksFree ResourcesThe Single-Cell World websiteSponsorshipPay me a Ko-FiThanks for listening.Cheers,Cátia This i...2023-11-2013 min
The Single-Cell World PodcastEp 38: QC’s that I Will Do Before A Spatial Transcriptomics ExperimentHi there!Would you like to know what quality control steps I think are essential before a spatial transcriptomics experiment? In my experience, these control steps can save you a lot of headaches.Listen now, and tell me if you agree.Useful LinksThe Single-Cell World websiteSponsorshipPay me a Ko-FiThanks for listening.Cheers,Cátia This is a public episode. If you would like to discuss this with other subscribers or get access to bonus e...2023-11-1316 min
The Single-Cell World PodcastEp.37: Exploring Single-Cell Spatial Transcriptomics with MERSCOPE®, Vizgen TechnologyDiscover the marvels of MERSCOPE®, a revolutionary platform that's reshaping the world of single-cell spatial transcriptomics. Think of it as Google Earth for genes; it allows us to directly map and quantify the spatial distribution of hundreds to tens of thousands of RNA species in individual cells. Dr. Jiang He, Vizgen’s Scientific Co-founder and Senior Director of Scientific Affairs explains everything about the MERSCOPE® technology! Listen now to learn more about single-cell spatial technology. And if you enjoy our podcast, don't forget to share with friends and subscribe to our podcast to listen...2023-11-0630 min
The Single-Cell World PodcastEp. 36: Sample Preparation For Spatial TranscriptomicsHi!Would you like to get a general idea about the sample preparation standard protocols for spatial transcriptomics experiments?Listen now 😉.Useful links:Youtube VideosFrozen Tissues PreparationFFPE Tissues PreparationHow to Cut Your Frozen Tissue Sample To Get Beautiful Sections?OCT Blocks PrepSupport this podcastKo-fiSponsorsThank you so much for listening.Cheers,Cátia This is a public episode. If you would like to discuss this with other subs...2023-10-3015 min
The Single-Cell World PodcastEp. 35: Parse Biosciences Evercode Technology: High-throughput single cell with combinatorial barcodingWelcome to this episode, in which I have a fascinating conversation with Charles Roco, the founder and CTO of Parse Biosciences. In our conversation, Charlie talks about Evercode single-cell technology. We look into the amazing flexibility of this technology, which allows us to go beyond the limits of gene expression analysis.Join us as we examine this intriguing high-throughput technology that 96-well plates allow for.Parse Biosciences useful links:WebsiteResources: application notes, whitepapers, data sets,etcetera..LinkedInTwitterInstagramCharles Roco LinkedIn and TwitterSupport this podcast...2023-10-2343 min
The Single-Cell World PodcastEp. 34: Samples For Spatial TranscriptomicsHi tere,Do you know that besides solid tissues, we can use other types of samples for Spatial Trancriptomics?Listen now to this episode podcast to know about all the sample types that can be used.Useful links:Support this podcast by buying me a coffee!Single-Cell World WebsiteThanks for listening!Have a lovely day,Cátia This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-10-1612 min
Ciência no FemininoEp. 9 | Células com personalidade com Cátia MoutinhoAs células, tal como as pessoas, têm características individuais. Ou devemos dizer o contrário: as pessoas, tal como as células, têm características individuais. (? 🤷♀️) A nível celular, está personalidade é importante para distinguir células no seu estado normal de células cancerígenas. A nível populacional, esta personalidade individual é importante para se trabalhar em equipa ou para gerir essa equipa!🧑🤝🧑 a Cátia Moutinho é bióloga e coach de cientistas e por isso com ela falamos de células e pessoas 👩🔬 2023-10-1444 min
The Single-Cell World PodcastEp. 33: PacBio’ MAS-Seq: Long-Read Sequencing For Single-CellIn this episode, Dr. Elizabeth Tseng, associate director product manager at PacBio, explains everything about the MAS-Seq kit, a game-changer for producing long-read libraries. With the backing of single-cell industry giant 10X Genomics, the MAS-Seq kit promises to redefine the future of single-cell RNA seq assays.Whether you're a scientist or just curious about the future of single-cell research, this episode is your guide to the cutting-edge advancements in the field. Tune in to learn, explore, and be amazed! Some interesting links:Dr. Elizabeth Tseng, Social Media: X and LinkdeInPacBio Resources:2023-10-0923 min
The Single-Cell World PodcastEp 32. Spatial Transcriptomics: Image-based methods (Live Instagram)Hi! This is our first live episode on Instagram: Spatial Trancriptomics Image-Based Methods🎙️In this live, I talk about the different methods that are in the base of some of the Spatial Transcriptomics technology. Listen now!Usefull LinksSupport this podcastJoin Our NewsletterNew WebsiteThanks for listening!Cheers,Cátia This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-10-0221 min
The Single-Cell World PodcastEp. 31: PIPseq, scRNAseq using a vortexHi there,In this episode, I talk with Kristina Fontanez, PhD, co-founder and Senior Vice president of Product Development at Fluent Biosciences. We talk about the groundbreaking PIPseq single cell technology.Step into the fascinating world of single-cell RNA sequencing as Kristina demystifies the revolutionary PIPseq method that is making waves in single-cell research.What makes PIPseq stand out? Tune in now!Fluent Biosciences useful links:WebsiteResources: application notes, whitepapers, data sets, etc..LinkedInTwitterKristina Fontanez LinkedInSupport this podcast by buying me a coffee!2023-09-2529 min
The Single-Cell World PodcastEp. 30: Spatial Transcriptomics MethodsHi! This is our first live episode on LinkedIn: Spatial Trancriptomics Methods🎙️In this live, I talk about the two main categories of Spatial Transcriptomics methods. Listen now!Usefull LinksSupport this podcastNew WebsiteThanks for listening!Cheers,Cátia This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-09-1820 min
The Single-Cell World PodcastEp. 29: SINGLE-CELL CONSULTATIONS: Question & Answers Part 2Today I decided to do something different. In this episode I share with you some frequent questions that I get on my talks and scientific consultations.Besides tell you that today is the last day to join our online course: Exploring the Cutting-Edge World of Single-Cell TechnologyAnd that next week I will be live on LinkedIn.In the meanwhile, luck for your single-cell experiments!Support this podcastThanks for listening!Cheers,Cátia This is a public episode. If you would like to discuss this w...2023-09-1110 min
The Single-Cell World PodcastEP. 28: Introduction To Spatial OmicsFirst Live Episode on X: Introduction to Spatial Omics 🎙️Get ready for an authentic experience—this is our debut live episode, streaming directly on X (Twitter)! Just a heads-up 👉🏻: we're keeping it real with unedited sound.In this unique live edition, we delve into the topic of Spatial Omics, focusing specifically on the fundamental reasons driving the development of this transformative technology. Don't miss it!Usefull LinksJoin now our online course: Exploring the Cutting-Edge World of Single-Cell TechnologySupport this podcastNew WebsiteThanks for listening!2023-09-0418 min
The Single-Cell World PodcastEp 27. How To Switch From Wet-Lab To Dry-Lab? Tips for single cell scientistsIn this episode, Dr. Ming “Tommy” Tang shares his story of changing from hands-on lab work to computer-based research. Tommy talks about the steps, challenges, and skills he learned along the way. Plus, find out how he got so many followers on X! This episode is not about methods or technology; it's about inspiring people in the single-cell research world. Give it a listen and get inspired by Tommy’s story. It can be a change in your own science journey! Some interesting links: Dr. Ming Tommy Tang Social MediaXLinkdeInChatom...2023-08-2837 min
The Single-Cell World PodcastEp. 26. SORT-seq: a single-cell RNA sequencing methodHi there!In this episode Mauro Muraro, CEO and Co-Founder of Single Cell Discoveries, enlightened me about SORT-seq. Join us as we talk about this single-cell RNA sequencing plate-based method that provides full transcripts information. Don't miss out on learning more about single cell cutting-edge technology. Listen now!Useful Links:SORT-seqSingle Cell DiscoveriesSingle Cell Discoveries Job OpportunitiesSingle Cell Discoveries LinkedIn & XMauro Muraro LinkedInSupport this podcastThanks for listening!Cátia This is a public episode. If you would like t...2023-08-1441 min
The Single-Cell World PodcastEp. 25: Illumina NGS and Single-Cell ExperimentsHi there!Dive into our most recent audio episode as we discuss Illumina Next Generation Sequencing (NGS) technology with our distinguished guest, Sophie Wehrkamp-Richter, product manager at the company. Immerse yourself in our vibrant debate about the critical role of DNA sequencing in single-cell research. Furthermore, we investigate critical aspects to consider when planning a single-cell experiment.Listen now and feed your curiosity!Useful Links:Single-Cell Illumina ebook and application notes Joint single- cell company: With 10x Genomicshttps://developer.illumina.com/news-updates/10x-genomics-chromium-single-cell-gene-expression-flex-data-now-available-on-illumina-basespace-sequence-hubhttps://developer.illumina.com/news-updates...2023-07-3125 min
The Single-Cell World PodcastEp. 24: Single-Cell MultiomicsHi there,Today's episode is about one of the trends on single-cell research: single-cell multiomics. Listen now to know more about the types os single-cell multiomics and the available kits that you can use.Support this podcastUseful Links:10X Genomics Multiome kitBD Biosciences KitsMission Bio MultiomicsBiolegend AntibodiesThanks for listening!Cátia This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-07-2411 min
The Single-Cell World PodcastEp. 23: Sequencing in single-cell experiments? What?Hi there,Today's episode is about an essential part of most of single-cell experiments and that some people are not aware: DNA sequencing.Listen now to know more about this topic. Support this podcastUseful Links:IlluminaBGIElement BiosciencesPacBIONanoporeThanks for listening!Cátia This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-07-1710 min
The Single-Cell World PodcastEp. 22: Talking About Single-Cell Data Analysis (Part 2)Hello there,Today's episode is Part two of “Talking about single-cell data analysis”, with Ayoub Lasri, founder of Single-Cell Analytics. In this episode, Ayoub explains better what type of services Single-Cell Analytics offers and how they can help us in our projects.Listen now!Useful Links:Single-Cell Analytics LTD WebsiteLinkedInTwitterAyoub Lasri contact: ayoub-lasri@singlecell-analytics.comSc-RNA ToolsSupport this podcastThanks for listening!Cátia This is a public episode. If you would like to discuss this with other...2023-07-1030 min
The Single-Cell World PodcastEp. 21: Talking About Single-Cell Data Analysis (PART 1)Hello there,In today's episode Ayoub Lasri, co-founder of Single-Cell Analytics, outlines the data processing method for single-cell RNA seq data in this episode. This podcast was split into two parts because there is so much helpful information to give. This is part one!Join us and see how much fun we have while discussing single-cell data analysis. I'm sure you're curious about whether my assumptions about single-cell data analysis as a wet-lab researcher are correct or incorrect.Useful Links:Single-Cell Analytics LTD WebsiteLinkedInAyoub Lasri contact: ayoub-lasri@singlecell-analytics...2023-07-0332 min
The Single-Cell World PodcastEp. 20. The Singulator: revolutionising tissue dissociationHi there,Join me for an exciting episode as I talk with John Bashkin, Chief Strategy Officer of S2 Genomics, about the Singulator - an automated tissue dissociator designed specifically for single-cell experiments.Discover how S2 Genomics is revolutionizing tissue dissociation at the single-cell level and how the Singulator enables us to get clean suspensions of cells or nuclei for our experiments. John provides fascinating insights into the Singulator's many applications, ranging from cancer research to organoids research.Whether you're interested in exploring different approaches on tissue dissociation or simply curious about the latest...2023-06-2627 min
The Single-Cell World PodcastEp. 19. The HIVE CLX Solution: Any Cell. Any Where. Any TimeHi there,In this episode I have a special guest, Irene Whitney the senior director of commercial strategy from Honeycomb Biotechnologies. We talk about their unique single-cell technology, the HIVE single-cell solution that allows us to study any cell, anywhere, and anytime.She also updates me about the new versions of the HIVE system, the HIVE CLX, explaining how it works and most important, the benefits of using this technology in single-cell research compared to traditional single-cell methods. Join me for this exciting episode as I explore the cutting-edge world of single-cell technology with...2023-06-1947 min
The Single-Cell World PodcastEp. 18: Pooling or Multiplexing Samples?Hi there,In this podcast episode, I will tell you the difference between pooling or multiplexing samples for a single-cell experiment. I will also explain the type of tags that we can use to multiplex samples: genotype, antibodies or lipids. Finaly I will let know about the benefits of using this strategies, like single-cell cost reduction. Here are the links for the products I mention at this episode:Genotyping (SNP) Arrays Examples: Illumina ; ThermoFisherBiolegend Antibodies & single-cell experimentsLipid Tags: Multi-seq (Sigma)👉🏻 Support this Podcast!Listen now!2023-06-1213 min
The Single-Cell World PodcastEp.17 Types of single-cell mRNA sequencing assaysHi there,In this podcast episode, I will tell you what three of single-cell mRNA assays we have available in order to study gene expression.👉🏻 Whoul you like to support this Podcast?Listen now!Cheers,CátiaDon't forget to subscribe to our newsletter and blog. This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-06-0512 min
The Single-Cell World PodcastEp.16: Mission Bio: single-cell targeted DNA & moreHi!In this exciting episode, I talk with Gema Fuerte, Mission Bio's Field Application Scientist Manager for EMEA. Gema takes us on an exciting journey through the world of single-cell genomics and the revolutionary technologies developed by Mission Bio to study individual cells in unprecedented detail.We discuss Mission Bio's goal of revolutionizing disease research at the single-cell level, as well as how their cutting-edge Tapestri platform and kits enable the detection of rare cell populations and genetic mutations that contribute to disease.Gema provides fascinating insights into Mission Bio's technology's many applications, ranging from...2023-05-1558 min
The Single-Cell World PodcastEp15: Single-Cell DNA Sequencing Methods: an overviewHi there,In this podcast episode, I will be talking about single-cell DNA. DNA is the information necessary to build our bodies. Alterations in DNA can cause changes in cell behavior, leading to diseases.To study DNA at the single-cell level, we can use diferent protocols. Some examples are the whole-genome or the targeted approaches, the scATAC-seq or the scDNA methulation protocols. Several commercial solutions are available and I will also mention them.Listen now!Cheers,CátiaUsefull LinksLAST DAY!! Single-Cell Course: Exploring the Cutting-Edge W...2023-05-0815 min
The Single-Cell World PodcastEp.14: Single-Cell Methods: an overviewWelcome!In this episode I will talk about the 3 main methods that were the base for the different single-cell sequencing technology. Below you can find all companies and products links that are mentioned during this episode. I hope this is useful for your and somehow can help. Thanks for listening!PLATE-BASED METHODS (e.g. commercial options)96 well Plates (BioRad)REPLI-g Advanced DNA Single Cell Kit (Qiagen)SMART-Seq Single Cell Kit (Takara Bio)Evercode Whole Trancriptome (Parse Biosciences)Micro, Nano or Pico WELL-BASED METHODS (e.g. commercial options)HIVE scRNAseq...2023-05-0111 min
The Single-Cell World PodcastEp. 13: Working With NucleiHi there!In this episode I talk with Dr. Luciano Martelotto, an expert on single-nuclei experiments. When we cannot isolate happy cells for our experiments, nuclei are the next option that we have. Listen now to learn more about this topic.Who is Dr. Luciano Martelotto?He is A/Professor at the Head of the Development Laboratory of the Adelaide Centre for Epigenetics (ACE), South Australia ImmunoGENomics Cancer Institute (SAIGENCI). Prior to joining ACE/SAIGENCI, he was the Scientific Director of the Single Cell Laboratory at Harvard Medical School, Department of Systems Biology, Harvard...2023-04-2458 min
The Single-Cell World PodcastEp. 12:Dead cells. Now what?In this podcast episode, I talk about different strategies that we can use when we have a lot of dead cells in our samples. Below there are links for the products and machines I mentioned on this episode.SINGLE-CELL COURSE, May 9, 2023Support this podcastDEAD CELL REMOVAL KITSMiltenyi BiotecAkadeumStem CellREAGENTSFicoll-PaqueLymphoprepHistopaqueOptiprepMACHINESLuna FLLeviCell This is a public episode. If you would like to discuss...2023-04-1712 min
The Single-Cell World PodcastEp.11: Sample Preservation Methods For Single-Cell ExperimentsHello there!Season two of The Single-Cell Podcast begins today.In this episode, I discuss the various preservation methods that can be used on samples for single-cell sequencing experiments.TopicsCryopreservationSnap-freezingFixationSpecific single-cell strategies developed by various single-cell sequencing technologies like Singleron, 10X Genomics, Honeycomb Biotechnologies, Parse BiosciencesThe courseExploring the Cutting-Edge World of Single-Cell Technology: An Introductory CourseFor more detailsPay me a kofi to support this podcastThank you! This is a...2023-04-1017 min
The Single-Cell World PodcastEp. 10: SINGLE-CELL CONSULTATIONS: Question & AnswersTo celebrate that The Single-Cell World podcast has already 10 episodes, I decided to do something different. In this episode I share with you some frequent questions that I get on my talks and scientific consultations.Besides that, tell you that this is the last episode of season 1. I will be back in 2 weeks after my holidays.In the meanwhile, luck for your single-cell experiments! This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-03-0617 min
The Single-Cell World PodcastEp. 09: Automated Tissue Dissociators For Single-Cell ExperimentsWhat about if I tell you that you don't have to do your tissue dissociations manually?In this episode I talk about different automated machines options that we can use to get single cells but also single nuclei. Next Thursday blog post you will find more information about this. BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.Gentle Mechanical Tissue Dissociators:gentleMACS Dissociator (Miltenyi)Singleron Python Tissue Dissociator (Singleron)Tissue Grinder (Fast Foward Discoveries)DSC-400...2023-02-2720 min
The Single-Cell World PodcastEp. 08: Solid Samples: phases of sample preparationWorking with solid samples?In this episode I will talk about the different phases of solid samples preparation.In next Thursday blog post you will find more information concerning solid samples. However I leave you here links from some of the websites and products that I mention during the podacast.BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.WEBSITES:Protocols.ioPubmedResearch GatebioRxivCELL COUNTERSLuna FL...2023-02-2017 min
The Single-Cell World PodcastEp. 07: Liquid Samples: what was used in single-cell sequencing?Working with liquid samples?In this episode I will tell you what samples were used on the published single-cell sequencing works. I will explain also the most common sample preparation methods used for these type of samples.In next Thursday blog post you will find more information concerning liquid samples. However I leave you here links from some of the products that I mention during the podacast.BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.PRODUCTS:...2023-02-1316 min
The Single-Cell World PodcastEp. 06: Testing ChatGPT Single-Cell KnowledgeEveryone is talking about ChatGPT! So I decided to invite it to my podcast.Are you curious to know its knowledge of single-cell research and technologies? Listen now 😉Find other FREE resources on www.thesinglecellworld.comI would love to know more about you. Follow me @singlecell_world (Instagram or Twitter). This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-02-0625 min
The Single-Cell World PodcastEp. 05: What kinds of samples can be used for single-cell sequencing? An overview.In this episode, I'll go over the different types of samples that can be used in single-cell sequencing experiments.Find other FREE resources on www.thesinglecellworld.comI would love to know more about you. Follow me @singlecell_world (Instagram or Twitter). This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-01-3008 min
The Single-Cell World PodcastEP.04: Questions you need to answer before planning a single-cell experiment.In this episode I talk about the checklist that I use while planning single-cell experiments. Basically are the essential questions that when answered help me deciding the best approach for the project. FREE GIFT: Single-Cell Planning ChecklistFind other FREE resources on www.thesinglecellworld.comI would love to know more about you. Follow me @singlecell_world (Instagram or Twitter). This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-01-2315 min
The Single-Cell World PodcastEP. 03: An Overview of Key Concepts for Single-Cell ExperimentsSooner or later, you would like to do a single-cell experiment. In this episode I explain you the key terms that you should know in order to avoid misunderstandings while planning your future experiment or project. E.g. libraries, UMI, reads...Find other FREE resources on www.thesinglecellworld.comI would love to know more about you. Follow me @singlecell_world (Instagram or Twitter). This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-01-1622 min
The Single-Cell World PodcastEP. 02: Why do researchers use single-cell technology?In this episode I will tell you what are the most common biological topics/questions that researchers want to answer using single-cell technology. They are just 3!Find other FREE resources on www.thesinglecellworld.comI would love to know more about you. Follow me @singlecell_world (Instagram or Twitter). This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2023-01-0910 min
The Single-Cell World PodcastEP. 01: Single-Cell? What is that?It seems that nowadays single-cell experiments are a must-have in all high-impact publications. But what is this technology? In this first episode I will explain you what is single-cell and how it differs from the standard experiments.Find other FREE resources on www.thesinglecellworld.comI would love to know more about you. Follow me @singlecell_world (Instagram or Twitter). This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit thesinglecellworld.substack.com2022-01-0205 min
The Genomics LabSingle Cell Technology Development and Spatial Omics with Dr Cátia MoutinhoWe've reached episode 20!!!In celebration of our twentieth episode, we are speaking today to Dr Cátia Moutinho about single cell sequencing and spatial omics - two up and coming techniques that everyone seems to be talking about at the moment. Cátia is Group Leader of the Single Cell Technology Development in the Garvan Institute in Sydney, Australia.The aim of Cátia's lab is to perform and optimise single-cell-related experiments, to develop the latest methods and approaches in molecular cellular genomics. We talk today about the importance of the development of these techniques, how...2021-05-051h 10