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The Rapidmicrobiology Podcast

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The Rapid Microbiology PodcastThe Rapid Microbiology PodcastEfficient Liquid Handling Critical in Responding to Surge EventsDenise Bolton (New Hampshire PHL) and Carol Loring (INTEGRA Biosciences) bring their public health expertise to this podcast’s discussion on how we can be better prepared for the next pandemic by using what we’ve learned from the last one. Timestamps: [5:00] - Liquid handling of COVID-19 samples before and after rapid instruments were employed [6:45] - INTEGRA's rapid instruments built to avoid repetitive strain injury [11:49] - Challenges in handling Monkeypox virus samples at public health lab [12:40] - Responding to outbreaks with rapid testing and instrumentation [15:28] - Be prepared, as there's no time in an e...2023-04-0417 minCause of Death - 100 Seconds to MidnightCause of Death - 100 Seconds to MidnightS4 E3: The Problem with Mary: The Story of Typhoid FeverTyphoid Show Notes:Cause of Death has a website! Come visit me at www.causeofdeath100secs.net. I also have an email address for the show at Jackie@causeofdeath100secs.net. Feel free to interact with me in either place!If you have questions for me for the Getting to Know Me episode, please contact me! Wanna know how I started the podcast? What my favorite color is? Was I always a geek? Just ask! I’ll answer them in a special episode coming in August!My Link Tree can be found at: https://linktr.ee/CauseofDeathpod2022-08-0637 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastFighting Epidemics with Molecular Diagnostics, From H7N9 to Monkeypox - Podcast To view transcript and visit supplier's page, please follow the link: https://www.rapidmicrobiology.com/news/fighting-epidemics-with-molecular-diagnostics-from-h7n9-to-monkeypox-podcast Timestamps: [1:37] - Responding to outbreaks quickly with molecular diagnostics [3:49] - Pioneers in RT-PCR and immunochromatographic assays [5:04] - Protecting lab technicians from pathogens and avoiding cross-contamination [8:00] - Increased awareness in prevention of infectious diseases with rapid and molecular diagnostics [9:30] - Bioperfectus' diagnostic portfolio and global partnerships  [12:07] - Quelling pathogens before they spread2022-07-1214 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastInfectious Disease Research: Reproducible Results Require Proper Pipetting - PodcastIn this podcast episode, our guests from Sartorius give advice on correct pipetting techniques that will avoid cell damage and reproducibility errors. Timestamps: [2:09] - Research papers without the pipette method leads to reproducibility errors [3:56] - Incorrect pipetting damages cells [4:38] - Reverse pipetting [6:46] - Optimizing pipetting steps for infectious disease research [8:43] - A pipette with smart features will help the researcher [12:05] - Sartorius Pipetting Academy Speakers:  Sandra Söderholm earned her PhD in Biochemistry from the University of Helsinki in 2016. In her thesis work, she investigated innate immune responses to In...2022-05-1613 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastGoodbye Autoclave, Hello ReadyStream® - A rapidmicrobiology PodcastThe ReadyStream® System is a revolutionary pre-enrichment workflow in food pathogen testing, removing the legwork and hands-on time conventional methods require. Listen to Scott Medley (Global Product Manager, Merck KGaA, Darmstadt, Germany) explain its novel features and benefits in this rapidmicrobiology podcast. Timestamps: [1:03] - The ReadyStream® System's functions and purpose [1:46] - Advantages over other rehydrate media on the market [3:16] - The story of its invention [5:07] - Energy and water savings to be made [8:11] - What happens if I don't use all the bag in one day? [9:02] - Media traceability [10:45] - IS...2021-12-1316 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastLateral-Flow Assay Development: It's Not Rocket Science But You Need Help - rapidmicrobiology PodcastIn this podcast, Andre Alfaro, Director of Assay Development at nanoComposix, lends expertise to medical device manufacturers entering the LFT market, particularly for COVID-19. Andre provides insights into this fast-growing sector and explains how nanoComposix can help every step of the way. Timestamps: [0:44] - nanoComposix's lateral-flow development services  [3:50] - Developing a SARS-CoV-2 lateral-flow test and bringing it to market  [4:58] - The beauty of antibody tests [7:47] - Why choose nanoComposix? [9:50] - How precious are gold particles? [13:32] - Health product agencies raising the quality standards bar  [15:20] - 1st gen assays that gave the rap...2021-12-1330 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastRapid Methods for SARS-CoV-2 Surveillance in Wastewater - PodcastListen to our latest podcast and learn how InnovaPrep's rapid method enabled GT Molecular to lead the field in wastewater epidemiology for SARS-CoV-2. Guests include Rose Nash (GT Molecular), Dave Goad and Dave Alburty (InnovaPrep). Timestamps: [1:11] - SARS-CoV-2's Achilles' heel (Rose Nash) [3:58] - Data for Action! (Rose Nash) [6:06] - Reducing turnaround times in wastewater epidemiology (Rose Nash) [8:24] - Choosing a concentration method (Rose Nash) [9:31] - The science behind the InnovaPrep Concentrating Pipette (Dave Goad) [14:20] - Sample sizes for wastewater epidemiology (Dave Goad) [16:25] - Recovery rates (Rose Nash & Dave Goad) [20:32...2021-08-1947 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastRapid Test Ensures Safety at Olympics Open-Water Events - a rapidmicrobiology PodcastIn this episode, our guest Tim Adams from Tecta-PDS talks about the success of the Tecta B16, the world's only approved portable rapid automated test for water, and explains how its user-friendly design and testing capabilities are knocking everything else out of the water. Timestamps: [1:40] - Explanation on why the Olympics committee chose the Tecta B16 for water testing [4:00] - How it works [7:55] - Differences between Tecta-PDS technology and other tests on the market (cost etc.) [9:56] - If no special training is required, how user-friendly is the Tecta B16? [12:47] - Origins of its...2021-07-2127 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastHow to Handle Environmental Monitoring in Isolators - A rapidmicrobiology PodcastIn this episode, Adele Gisselmann (Global Product Manager with Merck KGaA Darmstadt, Germany) describes the challenges of performing environmental monitoring in isolators, like manipulation with thick gloves and the effect of VHP, and explains how smart product design overcomes these challenges. Timestamps: [0:40] - Differences between environmental monitoring (EM) in isolator and cleanroom [2:05] - Performing EM with thick neoprene gloves [2:53] - Handling air samplers [3:38] - What impact does VHP have on EM? [5:11] - Neutralization of VHP residues [6:10] - Storing material for EM during the production run [7:25] - Taking away swabs and plates...2021-06-1008 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastWhy MAT Will Save Rabbits and Product RecallsIn this episode, Laure Robert (Global Product Manager with Merck KGaA Darmstadt, Germany) emphasizes the importance of pyrogen testing with MAT as a final release test, gives reasons for geographical differences in MAT standardization, and describes how to implement MAT in the lab, step-by-step. Timestamps: [1:00] - Contaminant Limit Concentration [3:00] -  Rabbit Pyrogen Test (RPT) or Monocyte Activation Test (MAT) [6:20] - Standardization of MAT [7:45] - Why perform a pyrogen test as well as BET? [11:03] - PyroMAT system comparison to competitors [14:04] - QC for pyrogens [17:10] - Implementing MAT in the lab S...2021-05-1721 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastShelf-Life Testing: Opening a Can of Worms - a rapidmicrobiology podcastThe food industry is constantly innovating – we have new protein sources like insects and algae, we have new packaging materials, we have new formulations reducing sugar and salt, other preservatives – how do manufacturers keep their shelf life dates relevant to their product changes? We’ll find out from food safety expert Linda Everis who currently manages the challenge test/shelf life studies at Campden BRI. Timestamps: [1:05] - 'Use-by' or 'Best-before' [4:18] - Challenges with extending shelf-life [7:05] - Determining safe shelf-life [8:26] - Determining quality & open shelf-life [13:11] - Listeria [16:20] - Consequences of a badly-designed challenge test2021-02-0223 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastGMP Annex 1: Changes in Microbial MonitoringIn this episode, Anne Klees and Adele Gisselmann (Global Product Managers with Merck KGaA, Darmstadt, Germany) unravel the New GMP Annex I (Draft 2020) and explain what effect its changes and new sections will have on environmental microbial monitoring.   Timestamps: [1:20] - Key Changes in Annex I [2:31] -  Rapid and Alternative Methods in Annex I [6:10] - Qualification of Recovery Rates for Air Samplers & Culture Media [7:45] - Validating Media for its Neutralization Efficiency [9:05] - Validating Air Samplers [14:40] - New Sections in Annex I (Aseptic Process Simulation/Media-fill etc.) [20:28] - How Merck KGaA, Darmstadt, Ge...2021-01-1523 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastContinuous Environmental Monitoring in New Annex IIn this episode, Andy Whittard (Managing Director at Cherwell Laboratories) and Gethin Jones (Managing Director at Pinpoint Scientific) lend their expertise to environmental monitoring in pharmaceutical manufacturing, focusing on the changes to continuous monitoring in the proposed Annex I.  Timestamps: [0:45] - Differences Between Air monitoring & Air sampling [4:53] - Difficulties in Setting up New System + Case Examples [9:28] - Importance of High-Collection Efficiency [11:20] - D50 Explained [12:29] - Sieve vs Slit-to-Agar Samplers [14:55] - Continuous Monitoring for Viables in New Annex I [19:30] - Minimizing Contamination Risk [21:52] - Adoption of Rapid Methods vs Reconfiguration of E...2021-01-1329 minThe Rapid Microbiology PodcastThe Rapid Microbiology PodcastOne Lab’s Experience of Testing for LegionellaIn this episode on Legionella testing, we have Ray Petrisek, a principal investigator in the discovery of Legionella in the ‘70s, who gives advice on testing and sampling for Legionella and the limitations and advantages of test kits in today’s market.   Timestamps: [4:47] - Legionella discovery in the lab [12:13] - Testing for Legionella in Potable water [13:51] - Sampling: “Look for the biofilm, and you’ll find Legionella” [21:21] - Limitations with plate-based method & PCR [29:54] - Preferred test method; IDEXX Legiolert [35:13] - Ongoing problems with Legionella Speaker: Raymond Petrisek serves as a senior microbiologist and infecti...2020-12-2243 min