Shows
Beyond A Million193: Billion-Dollar Founder Shares His Secret Weapon From the Swiss Army – 8FEWhat could a Swiss Army officer possibly teach you about building and selling companies worth hundreds of millions? Turns out – everything. Today's guest, Sam Goodner, took the discipline and precision he learned in the Swiss Army and applied it to entrepreneurship. He founded Catapult Systems, scaled it through two major recessions, and eventually sold it after 20 years. Later, he helped take Flash Parking from a stuck $3M startup to $100M in revenue and a billion-dollar valuation. Now a mentor at Capital Factory and author of Like Clockwork: Run Your Business with Swiss Army Pr...
2025-09-111h 06Beyond A Million193: Billion-Dollar Founder Shares His Secret Weapon From the Swiss Army – 8FEWhat could a Swiss Army officer possibly teach you about building and selling companies worth hundreds of millions? Turns out – everything. Today's guest, Sam Goodner, took the discipline and precision he learned in the Swiss Army and applied it to entrepreneurship. He founded Catapult Systems, scaled it through two major recessions, and eventually sold it after 20 years. Later, he helped take Flash Parking from a stuck $3M startup to $100M in revenue and a billion-dollar valuation. Now a mentor at Capital Factory and author of Like Clockwork: Run Your Business with Swiss Army Pr...
2025-09-111h 06
bccpbccp7 w/ Annie Goodner (Part Two)Annie Goodner - at her dining room table
This episode was recorded soon after Part One, and is a supplement on stuff like: The world of Alison Roman, Sohla and the Bon Appetits, Annie's adoration of Amiel, Brad Leone's locks, Products ft Edgar - (Schogetten choc, Weleda trash, Louis Widmer endorsement, aggravating anthroposophes), Doutzen Kroes' city visit - Laura Dols, Planet Hollywood and Bruce Willis, the new Amsterdam Hard Rock Café', Brad Pitt, Fresh Air with Terry Gross, Jenny Slate, and Marc Maron.
links to mentioned videos below
Alison Roman's S...
2020-05-2744 min
bccpbccp6 w/ Annie Goodner (Part One)Annie Goodner - on a daily walk through Vliegenbos, Amsterdam Noord
Camouflage, Hilton Als, Brenda Shaughnessy, Annie's new clogs from Margaret Howell London, the tags on the Vliegenbos corral for pedestrians and not cyclists, Ocean Vuong, Seth Meyers, Stephanie Meyer, Number zero dogs, Glass pineapple duos, Lea Thau's Strangers and The Cape, Ragnar Kjartansson, an introduction to Lea Thau, The Believer, 45.y.o parties with designers, encounters at Tegenboschvanvreden, The Dig, Bye bye Bernie, Seeking Derangements, Grey Wolf Press, Leslie Jameson's Empathy Exams, Gillian Rose Love's Work, Troye Sivan's feet
2020-05-2058 min
Genomics RevolutionMERS - A Recent, Ongoing But Overshadowed Coronavirus EpidemicGenomics RevolutionGuest Hosts: Matthew Hecker & Miranda MordueEpisode 54: MERS Coronavirus- Middle East Respiratory Syndrome-related coronavirus Hello, and welcome to Genomics Revolution. This is Matthew Hecker, and this is Miranda Mordue, bringing you in from the Hiram College Genetics course of 2020. As we stand in April 2020, the world is currently in a state of flux with COVID-19, a novel coronavirus that is taking the world by storm, and not in a good way. In light of COVID-19, our topic for today is another variety of novel coronavirus, MERS...
2020-04-2404 minGenomics RevolutionSARS - The First Coronavirus Near-PandemicGenomics RevolutionGuest Hosts: Denise Hart & Madyson MorrisEpisode 53: SARS Coronavirus Welcome to Genomics Revolution. This is Denise Hart and Madyson Morris from the 2020 Hiram College Genetics course hosting this episode on the SARS coronavirus. The acronym SARS stands for Severe Acute Respiratory System.1 To distinguish between the virus and the disease it causes, we will call the virus the SARS coronavirus and the disease SARS from here on out. Today, we will discuss the SARS coronavirus genome, as well as the outbreak that occurred across the globe from 2002 to 2003.
2020-04-2405 minGenomics RevolutionZika - The World Traveler Builds Up to a ScareGenomics RevolutionGuest Hosts: Keegan Rankin and Torey CowardEpisode 52: Zika Script:Keegan: Hello welcome to the podcast! I'm Keegan! Torey: And I’m Torey Coward! Keegan: And we are here today to talk to you about the Zika Virus. I’ll start us off with some general information. The Zika Virus belongs to a group of viruses known as flaviviruses. Flaviviruses are single-stranded RNA viruses encapsulated by a protein coat. Some of Zika Virus’ closest relatives include Yellow Fever Virus and West Nile...
2020-04-2402 minGenomics RevolutionOut of the Woods: Ebola as a Zoonotic Viral DiseaseGenomic RevolutionGuest Hosts: Alexus Acton & Rachna PrasadEpisode 51: Ebola Script:Rachna: Welcome to Genomics Revolution. This is Alexus Acton and Rachna Prasad from the 2020 Hiram College Genetics course hosting this episode on the Zaire Ebolavirus. This virus causes the disease ebola that originated from human animal contact, most likely from a bat (1). The Ebola Virus Disease, or EVD for short, was first discovered in 1976 with 2 consecutive outbreaks of fatal hemorrhagic fever in Central Africa. The first outbreak was in the Democratic Republic of Congo, which was formerly called Z...
2020-04-2405 minGenomics RevolutionAlexander the Great, Watch Out for the Dead Birds!Genomics RevolutionGuest Hosts: Alysa Giudici & Rachel JerkinsEpisode 50: West Nile Fever Script:Rachel: Hey everybody, and welcome to another episode of Genomics Revolution. This is Rachel Jerkins and Alysa Giudici (Guh-Dee-Cee), here to talk about the West Nile Virus. Rachel: The West Nile Virus comes from the flavivirus genus and the family flaviviridae. In 1937, the virus was first discovered in the West Nile area of Uganda in Africa. It is a single-stranded RNA virus around 11kbp in size with stem loops on the 5’ and 3’ ends. T...
2020-04-2403 minGenomics RevolutionThe Insane Zoonotic Trip That is RabiesGenomics RevolutionGuest Hosts: Giselle Bahena & Diamond Johnson Episode 49 – Rabies Lyssavirus Script: Welcome to Genomics Revolution. This is Giselle Bahena and Diamond Johnson from the 2020 Hiram College Genetics course hosting this episode covering the Rabies lyssavirus. As the scientific name of this virus implies, the disease that results from such infection is commonly known as Rabies. This disease has been around since antiquity and the earliest writings about it was found in 300BC in Mesopotamia.2 It was discovered through the infectious bite from one animal to the other. The biggest red...
2020-04-2404 minGenomics RevolutionUpdate on Current HIV TreatmentsSome More Information on How HIV Causes AIDS & on New Drugs:https://m.youtube.com/watch?v=BADDj82oces (Battle between HIV & Immune System video from Nature Reviews)https://www.npr.org/sections/health-shots/2019/05/30/727731380/old-fight-new-front-aids-activists-want-lower-drug-prices-now (National Public Radio (NPR) segment “AIDS Activists Take Aim at Gilead to Lower Price of HIV Drug PrEP”https://www.npr.org/sections/health-shots/2019/06/11/731350223/expert-panel-recommends-wider-use-of-daily-pill-to-prevent-hiv (NPR segment “Expert Panel Recommends Wider Use of Daily Pill to Prevent HIV”https://www.npr.org/sections/health-shots/2019/12/04/784733337/hiv-prevention-drugs-are-available-for-free-how-do-you-get-them (NPR segment “HIV Prevention Drugs are Available for Free: How Do You Get Th
2020-04-2404 minGenomics RevolutionTrailer: Zoonotic Viral DiseasesBrad sets the table for the last set of 2020 Hiram College Genetics course guest podcasts.
2020-04-2400 minGenomics RevolutionHIV & AIDS - The Killer That Robs Us of Our Immune DefensesGenomics Revolution Guest Hosts: Emily Harris & Tim MurtonEpisode 48: HIV & AIDS Script: Welcome to Genomics Revolution! This is Emily Harris and this is Tim Murton. We are from the 2020 Hiram College Genetics course, and we are hosting this episode on the genome of Human Immunodeficiency Virus, or HIV. HIV targets a host’s immune system and causes it to fail. This complication is referred to as HIV infection, and can eventually develop into acquired immunodeficiency syndrome, or AIDS, which is the most advanced stage of HIV infection (1). This...
2020-04-2304 minGenomics RevolutionHPV - The Trigger Behind Several CancersGenomics RevolutionGuest Hosts: Sheree Nobles & Joshua GregoryEpisode 47: Human Papillomavirus (HPV) Script:Josh- Hello everyone, and welcome to this episode of Genomics Revolution! We’re your guests hosts today, Sheree Nobles and Joshua Gregory. Today we’ll be talking about Human Papillomavirus, or HPV as it’s commonly known. This is a sexually transmitted infection, and to keep on topic with this year’s theme, it’s a virus. Sheree- There isn’t a scientific name for it, so much as a bunch of sc...
2020-04-2304 minGenomics RevolutionHepatitis C - Until Now, the Silent Hepatitis EpidemicGenomics Revolution Guest Hosts: Abbey Anderson & Samantha Mansfield Episode 46: Hepacivirus (Hepatitis C) Script: Hello! This is Sammie and Abbey coming at you live from a safe social distance! Today we are here to talk to you about Hepacivirus which is commonly known as Hepatitis C virus. We are going to spend some time talking about when and how the virus was discovered, the reasons that we should care about this virus, the genome of Hepatitis C, and key findings about HepC virus. Sammie: Every story starts somewher...
2020-04-2305 minGenomics RevolutionTrailer - Sexually Transmitted & Blood-borne VirusesBrad jumps in with a trailer for 4 episodes dealing with viruses that are transmitted by sex and transfer of bodily fluids.
2020-04-2300 minGenomics RevolutionHepatitis B - B for Blood and Other Bodily FluidsGenomics RevolutionGuest Hosts: Ciara Love & Cara KatzendornEpisode 45: Hepatitis B VirusScript: Hi, my name is Ciara and today we’re going to talk about a virus called Hepatitis B. This virus is a type of species in the orthohepadnavirus genus as well as a member of the hepadnaviridae family. This virus causes a world-wide known disease called Hepatitis B or HBV. HBV causes liver cancer and the vaccine that was invented for Hepatitis B was one of the first anti-cancer vaccine. The virus was discovered in 1965 by Dr. Baruc...
2020-04-2303 minGenomics RevolutionSoon We Can Say Goodbye to PolioGenomics RevolutionKiyana Caver & Brittany WeaverGenetics Podcast TranscriptEpisode 44: Poliovirus Script: Hello, I am Kiyana & I am Brittany and we will discuss the virus poliovirus which causes polio. Polio can also be known as poliomyelitis. It stems from the Greek meaning of an inflammation of the gray matter. In the early 20thcentury there weren’t many diseases that had parents worried about the health and wellbeing for their children. However, poliomyelitis is something that parents were fearful of their children getting. Poliomyelitis was a disease that was more co...
2020-04-2300 minGenomics RevolutionViral Pathogen No More - See Ya SmallpoxGenomics RevolutionMit Patel and Andrew PembertonEpisode 43: Variola Virus (Cause of smallpox)Script: Welcome to the Genomic Revolution Podcast! This is Mit Patel and Andrew Pemberton from the 2020 Hiram College Genetics course hosting this episode on an orthopoxvirus known as the Variola virus. This virus causes the disease that many of our parents or grandparents may have gotten when they were younger but rather for us millennials we are vaccinated for it. The variola virus or VARV causes the disease smallpox. However, there are some mysteries as to how it originated. According to Igo...
2020-04-2306 minGenomics RevolutionTrailer - Wiping Viruses OutBrad jumps in with a short trailer introducing some viruses that we have conquered or nearly conquered.
2020-04-2301 minGenomics RevolutionType B - The Other InfluenzaGenomics RevolutionBrianna Bays and Melika King Episode 42Influenza Type B Virus Script: Welcome to Genomics Revolution. This is Brianna Bays and Melika King from the 2020 Hiram College Genetics course hosting this episode over Influenza virus type B commonly known as the flu virus. The common symptoms are fever, muscle aches, headache, lack of energy, sore throat, and nasal congestion (1). Complications can rise due to immune status and underlying medical conditions (1). In 1892 two physicians Richard Pfeiffer and Shibasaburo Kitasato worked at a lab in Berlin...
2020-04-2303 minGenomics RevolutionInfluenza Type AGenomics RevolutionBenjamin Blake Erman and Ciza SadokeEpisode 41: Influenza Virus Type A Script:Hello and welcome to Genomics Revolution. Today’s episode is hosted by me, Blake Erman and my partner, Ciza Sadoke, which we’ll hear from later on in the podcast. We are from Brad Goodner’s 2020 Hiram College Genetics course and today we will be focusing on the virus Influenza Type A. This virus causes Influenzae which can give you the symptoms of; a fever, chills, headaches,muscle aches, feeling tired and weak, sneezing, stuffy or runn...
2020-04-2305 minGenomics RevolutionMeasles - Will We Ever Be Done With It?Genomics Revolution PodcastAllison Slutz & Cole FilerEpisode 40: Measles & Morbillivirus Hi all. Welcome back to another episode of Genomics Revolution. Today you are hosted by Cole Filer and myself, Allison Slutz. We are part of the 2020 Hiram College genetics course and will be talking about the Measles morbillivirus, the virus that causes the measles. The genus that morbillivirus is apart of, is the family Paramyxoviridae (1). The measles virus genome typically consists of 115,894 nucleotides, and encodes for 8 proteins: 6 structural (N, P, M, F, H, L) and 2 non-structural (V and C...
2020-04-2303 minGenomics RevolutionNo Chicken in Chickenpox or ShinglesZach Walker & Alainna Conroy Virus: Varicella zoster virusDisease: Chickenpox/ Shingles Welcome to today’s episode of Genomics Revolution. I am Zach Walker and am here with Alainna Conroy and we will be discussing the varicella zoster virus. We will be referring to this as the VZV. BACKGROUND/ DISCOVERY:In 1888, Von Bokay first observed that the VZV was related herpes zoster virus or shingles when children contracted VZV from adults who had shingles. Then in 1954, Thomas Weller took cell cultures from VZV lesions to sci...
2020-04-2304 minGenomics RevolutionThe Viral Age BeginsGenomics Revolution PodcastEpisode 38: The Viral Age BeginsHost: Brad Goodner, Professor of Biology & Biomedical Humanities at Hiram College Transcript:Welcome back. Now that we have a basic definition of a virus – a RNA or DNA genome surrounded by a protein shell called a capsid, let us put that definition into some historical context. When did we humans come to realize that viruses existed? When did we realize that they were different from cellular life forms? The Viral Age began, in terms of human recognition, around 120 years ago. Yet, it was n...
2020-04-2205 minGenomics RevolutionA New Season, A New NormalGenomics Revolution PodcastEpisode 37: A New Season, A New NormalHost: Brad Goodner, Professor of Biology & Biomedical Humanities at Hiram College Transcript:Welcome to our 2nd season of Genomics Revolution. I am your host, Brad Goodner. Along with this new season of podcasts comes a new normal that all of us are dealing with – the COVID-19 pandemic. I hope that you and your loved ones are not just safe and healthy, but that you are finding new ways to engage with the world. I know here in northeast Ohio where I teach...
2020-03-2103 minGenomics RevolutionSurvey of Genomes - Saccharomyces cerevisiaeWelcome to Genomic Revolution. This is Curtis Swearingen from the 2019 Hiram College Genetics course hosting this episode on the genome Saccharomyces cerevisiae S288C. This strain is from the fungal division Ascomycota and the family Saccharomycetaceae. Saccharomyces cerevisiae is an eukaryotic microbe. More specifically, it is a globular-shaped, yellow-green yeast belonging to the Fungi kingdom, which includes multicellular organisms such as mushrooms and molds. Natural strains of the yeast have been found on the surfaces of plants, the gastrointestinal tracts and body surfaces of insects and warm-blooded animals, soils from all regions of the world and even in aquatic env...
2019-07-1206 minGenomics RevolutionSurvey of Genomes - Even Most Eukaryotes are MicrobialWelcome back to Genomics Revolution. Brad Goodner jumping back in to our Survey of Genomes guest-hosted by students from the 2019 Hiram College Genetics course. You have probably been wondering “hey, when are they going to talk about the human genome?” We will get to it in time, but I am big believer that we humans need to see the biological world through a non-human dominated lens. Life on Earth, contrary to what our eyes tell us, is actually dominated by microbes – in cell number, in biomass, and in diversity. That is even true for the major branch of the tree of lif...
2019-07-0604 minGenomics RevolutionSurvey of Genomes - Fusobacterium nucleatum ATCC 25586Hey guys welcome to this guest podcast of Genomics Revolution hosted by yours truly Hunter Jenkins of the 2019 spring Genetics class of Hiram College. In this episode, we will be zeroing in on the specific strain Fusobacterium nucleatum ATCC 25586 which I will now refer to as F. Nucleatum. This strain is a subspecies of Fusobacterium nucleatum named nucleatum which can be found in the human oral cavity, (cough) your mouth. This organism is from a Genus of Fusobacterium, and family Fusobacteriaceae which I believe previously resided under the family of Bacteroidaceae. F. nucleatum is closely related to Fusobacterium necrophorum whic...
2019-05-2104 minGenomics RevolutionSurvey of Genomes - Buchnera sp. APSWelcome to Genomics Revolution. This is Hannah Mann from the 2019 Hiram College Genetics course hosting this episode on Buchnera aphidicola sub species Acyrthosiphon Pisum, or Buchnera sp. APS. Named after Paul Buchner, a pioneer in the field of symbiotic microbiology of sap sucking insects.Buchnera sp. APS is a type of microbe found in the gut of pea aphids (Acyrthosiphon Pisum). The endosymbiotic relationship between these two organisms goes back over 160 million years and is tied to the creation of 5 essential amino acids that two organisms share. The pea aphids maintain this relationship by passing the bacteria...
2019-05-2005 minGenomics RevolutionSurvey of Genomes - Thermotoga maritima MSB8Hello all, this is Tae’lor Jones from the 2019 Hiram College Genetics course, and today on this episode I will reveal some interesting information on the genome Thermotoga maritima MSB8, which I will refer to as T. maritima from here on out. So, sit back and fasten your seat belts because you’re going to learn all the incredible wonders of this amazing organism just within 3-5 minutes! T. maritima, a non- spore- forming, or in other words non-pathogenic, rod -shaped bacterium belonging to the order Thermotogales, was originally isolated from a geothermal heated marine sediment near Vulcano, Italy, and...
2019-05-1805 minGenomics RevolutionSurvey of Genomes - Deinococcus radioduransWelcome to Genomics Revolution! My name is Nicole Ryman and I will be hosting this episode to discuss the genome of a bacterium with a Guinness World Record! Deinococcus radiodurans claimed the record for “Most radiation-resistant lifeform” by being capable of withstanding 1.5 million rads of gamma radiation, which is about 3,000 times the lethal amount to kill a human. The bacterium is capable of not only surviving, but also reproducing in environments that would be lethal for any other organism. Deinococcus radiodurans R1 was the first strain of deinobacteria to be discovered by Arthur W. Anderson in 1956 after observing red bacte...
2019-05-1604 minGenomics RevolutionSurvey of Genomes - Chlorobium tepidum TLSWelcome to Genomics Revolution. This is Kerington Vickers from the 2019 Hiram College Genetics course hosting this episode on the genome of Chlorobium tepidum TLS. I will call this C. tepidum from here on out. This strain of thermophilic green sulfur bacteria was isolated from acidic high sulfide hot springs on North Island, New Zealand (3). This organism is part of the Chlorobiaceae family which are also called green sulfur bacteria. Green sulfur bacteria use sulfide ions as electron donors for photosynthesis. They are known to be found in depths of up to 145 m in the Black Sea, with l...
2019-05-1506 minGenomics RevolutionThe True Extreme Athletes - ExtremophilesWelcome back to Genomics Revolution. This is Brad Goodner. I freely admit that I am a sports nut. I love watching humans compete against themselves and each other under stressful conditions. I also grew up intrigued by extreme athleticism in non-human animals – the world’s fastest mammal or longest-distance migrant. I don’t think I am alone in being intrigued by survival and even more so by high performance under conditions that seem way beyond the norm. I bring this up today because when it comes down to it, microbes are the ultimate extreme athletes (1).Many of the microbia...
2019-05-1508 minGenomics RevolutionI Don’t Think It Means What You Think It MeansBrad Goodner here. It is great to be back with you after some more student-hosted episodes of Genomics Revolution. Did the title of today’s episode bring a chuckle or a smile to your face? It is one of many memorable lines from one of my favorite movies – The Princess Bride. Vizzini, the big-brained Sicilian kidnapper says “Inconceivable” every time the so-called “Man in Black” makes it past a huge obstacle. Vizzini’s hired swordsman, the Spaniard Inigo Montoya finally turns to Vizzini and says “you keep using that word. I don’t think it means what you think it means.”Inigo M...
2019-04-1807 minGenomics RevolutionAn Interlude on Pathogen GenomesHey, Brad Goodner here. It has been several episodes since I last got the chance to talk with you. I hope you are enjoying the Survey of Genomes episodes guest hosted by students from my 2019 Hiram College Genetics course. There are more of those episodes to come, but I wanted to jump in and provide some context. So far, 4 out of the 7 student-hosted episodes have dealt with human pathogens. Why sequence their genomes? Don’t we just want to kill pathogens in order to cure infectious disease?No doubt about it, healthcare practitioners not only want to diagnose...
2019-04-1604 minGenomics RevolutionSurvey of Genomes - 2 Bifidobacterium StrainsWelcome to Genomics Revolution. This is guest host Stephanie Cipa from the 2019 Hiram College Genetics course. I’m going to be talking about two strains of bacteria today; Bifidobacterium longum NCC2705 and Bifidobacterium longum ssp. longum 35624, previously known as Bifidobacterium infantis 35624. From here out I will be referring to them as B. longum 2705 and B. longum 35624. Lets dive right into it! B. longum has one circular chromosome made up of about 2,256,646 base pairs. In B. longum 2705 this creates up to 1,725 proteins. To really understand this bacteria I’m going back to the start of all our human lives...
2019-03-2905 minGenomics RevolutionSurvey of Genomes - Caulobacter crescentusHello everyone, My name is Tim Stucky and today I’ll be taking you through the genetics of Caulobacter crescentus. Caulobacter was first proposed in 1935 by Henrici and Johnson, two researchers who found the bacteria in microscope slides with samples from a freshwater lake. It is considered a stalked organism, meaning it has a long tubelike extension in its mature form. This organism fit into the Caulobacteriales which was a newly found order for bacteria at the time of its discovery. It is a gram-negative bacteria that lives in nutrient poor areas of fresh water bodies. There are two main st...
2019-03-2903 minGenomics RevolutionSurvey of Genomes - Vibrio cholerae strain El Tor N16961Welcome to Genomics Revolution. This is Danielle Vincent from the 2019 Hiram College Genetics course, and I will be your host for this episode on the genome of Vibrio cholerae El Tor N16961. Now, just to make things a bit easier, I’ll go ahead and refer to this organism as V. cholerae for the remainder of this episode. V. cholerae is a gram-negative, gamma-Proteobacterium that you may already recognize as it’s the bacterium that causes the disease Cholera (1). In 1854, during the third pandemic of Cholera, an anaethesiologist named John Snow theorized that this, at the time, unknown, disease was...
2019-03-2905 minGenomics RevolutionSurvey of Genomes - Drosophila melanogasterHello, and welcome back to genomics revolution. I am Kaitlyn Morse a guest scientist here today to talk about the organism Drosophila melanogaster, or what we know as the fruit fly. We may think of this insect as a pest but, it has been one of the most useful organisms in a biology/ genetics lab to date. You may be asking how is that possible? A small fly? Let’s discuss why Drosophila has become so important.The discovery of Drosophila occurs in 1830 but, the first time Drosophila became a popular organism to study was back in 1910 in...
2019-03-2904 minGenomics RevolutionSurvey of Genomes - Schizosaccharomyces pombeWelcome to Genomics Revolution. I am Brett Bentkowski, from the 2019 Hiram College Genetics Course and it is my pleasure to host this episode on Schizosaccharomyces pombe, the so called fission yeast. Fission yeast was first reported in 1893, by Paul Lindner, who isolated if from East African millet beer. It gets its species name pombe from the Swahili word for beer: pombe. It then went on to be used by Urs Leupold for genetic study, and then by Murdoch Mitchison for studying the cell cycle, both around the 1950s. S. pombe’s common name, fission yeast, comes from how the ce...
2019-03-2904 minGenomics RevolutionSurvey of Genomes - Pseudomonas aeruginosaWelcome back to Genomics Revolution, this is Kiara Jeffrey from the 2019 Hiram College Genetics course hosting this episode on the genome of Pseudomonas aeruginosa strain PAO1, which I will call P. aeruginosa from now on. This rod-shaped, gram-negative bacterium belongs to the Bacteria class gamma-Proteobacteria and the family Pseudomonadaceae (2). P. aeruginosa was first discovered in 1882 when a French bacteriologist and chemist, Carle Gessard, noticed a blue and green color develop on the bandages of two of his patients’ wounds. Through an experiment, he found that this odd color development was the result of the bacterium’s water-soluble pigments, which turn...
2019-03-2905 minGenomics RevolutionSurvey of Genomes - Ralstonia solanacearumWelcome to Genomics Revolution. This is Sam Hitchcock from the 2019 Hiram College Genetics course and I will be hosting this episode on the genome of Ralstonia solanacearum GMI1000. R. solanacearum is a beta-proteobacterium, which is a class of proteobacteria that occupy diverse environments as pathogens living within hosts. R. solanacearum is no different, this pathogen is soil borne and infects roots. Different strains of R. solanacearum are able to attack 200 different hosts. The first strain of R. solanacearum to have its genome sequenced was Ralstonia solanacearum GMI1000, this took place in 2002 (3). The complete genome was sequenced in order to...
2019-03-2903 minGenomics RevolutionSurvey of Genomes - Mycobacterium tuberculosisWelcome to Genomics Revolution. This is Anna Pallante and this episode will focus on the genome of Mycobacterium tuberculosis H37Rv. Mycobacterium tuberculosis is a pathogenic bacteria from the family Mycobacteriaceae that was discovered in 1882 by Robert Koch (1). Koch was able to isolate the bacteria from tissue samples from animals that were suffering from tuberculosis. However, he had to develop and use new staining and culturing techniques in order to do so. When the tissue samples were stained with methylene blue, M. Tuberculosis turned bright blue while the surrounding tissue and other bacteria were all turned brown (1). All infected...
2019-03-2904 minGenomics RevolutionSurvey of Genomes - Campylobacter jejuniWelcome to Genomics Revolution. This is Ka Shing Allan So from the 2019 Hiram College Genetics course hosting this episode on the genome of Campylobacter jejuni, or C. jejuni.C. jejuni falls under the Campylobacteraceae family, and the Campylobacter genus. Campylobacter jejuni was discovered by Theodor Escherich. In 1886, Escherich was studying stool specimens and large intestinal mucous correlated to diarrhea in kittens and in neonates. He published his result in “The Intestinal Bacteria of the Infant and Their Relation to the Physiology of Digestion”. Escherich would grow cultures from infant stool samples and he successfully identified 19 different bacteria includin...
2019-03-2904 minGenomics RevolutionSurvey of Genomes - Treponema pallidumMy name is Daijah Sek, and on today’s episode I will be discussing Treponema pallidum which many of you may already be familiar with. In 1905, scientists Schaudinn and Hoffman identified this spiral-shaped bacterium as a causative agent of syphilis. However, we now suspect with more recent research done that this may have originated and been present as far back as in the Columbus era in the 1400s in Europe. It’s subspecies can cause yaws and bejel in restricted regions of the world. Though they are more constrained to tropical climates.As said by Fraser et al...
2019-03-2903 minGenomics RevolutionSurvey of Genomes - Clostridium perfringensWelcome to Genetics Revolution. This is Ashley Redman from the 2019 Hiram College Genetics course hosting this episode of clostridium perfringens. Clostridium perfingens, CP for short, is a gram positive anaerobic, spore-forming, bacterium known to be the most widely distributed pathogen in nature. It is commonly found in soil, sewage, raw meat, and in the intestines of animals and humans as a member of the normal flora. The clostridium perfringens strains are classified into five groups, types A-E on the basis of their production of the four major toxins known as the alpha, beta, epsilon, and iota-toxins. Clostridium perfringens causes...
2019-03-2905 minGenomics RevolutionSurvey of Genomes - E. coli O157:H7Welcome to Genomics Revolution. I’m Taylor Yamamoto from the 2019 Hiram College Genetics course hosting this episode on the genome of the bacteria Escherichia Coli O157:H7. I will be calling it E. Coli from now on. This strain of E. Coli is the most harmful strain to humans because it produces a toxin, called Shiga toxin, that causes bloody diarrhea and hemolytic-uremic syndrome, which is when red blood cells get damaged, and then cause a blockage in the kidneys. This can lead to life-threatening kidney failure. Comparatively, nonpathogenic E. Coli often inhabit the human gut without any adverse af...
2019-03-2903 minGenomics RevolutionSurvey of Genomes - Yersinia pestis strain KIMWelcome to Genomics Revolution. My name is Alexis Polcawich from the 2019 Hiram College Genetics course hosting this episode on the genome called Yersinia Pestis. This is a gram negative, nonmotile, rod-shaped coccobacillus bacteria that does not form spores and is a facultative anaerobe. This bacterium was discovered in 1894 by Alexandre Yersin in Hong Kong. Yersin was able to isolate the bacterium in culture and identified it via microscope. Jean-Paul Simond was Yersin’s inspiration to do so as in 1898 he discovered that bacteria was transferred from rodents by flea bites, and Yersin thought it would be beneficial to learn mo...
2019-03-2904 minGenomics RevolutionSurvey of Genomes - Halobacterium NRC-1Hello all, today I will be talking about Halobacterium NRC-1, or ATCC 700922. Halobacterium is a genus of Halobacteriaceae. The Halobacterium species is actually not a bacteria, however, it belongs to the archea domain. Halobacterium was originally studied in the 1960s, brine shrimp, who are filter feeders, would consume the Halobacterium, in turn, flamingos would eat the brine shrimp, giving them the pigment that they are. The genus Halobacterium ("salt" or "ocean bacterium") consists of several species of the Archaea with an aerobic metabolism which requires an environment with a high concentration of salt; many o...
2019-03-2903 minGenomics RevolutionSurvey of Genomes - Aeropyrum pernix K1Hi my name is Brayla Stokes and today will be talking about Aeropyrum pernix K1 and this genome is a species of Archaea. It was the first crenarcheote which is just a category of Archaea and first aerobic member of Archaea with a complete genome sequence. This organism helped us link the relationship between the three domains of life. In 1999 Aeropyrum pernix was discovered while heating and vent marine water sediments in Japan. It is circular in shape and its diameter is 1 micrometer. Its genome is 1,669,696 base pairs in size. We see a growth response starting at 70 degrees Celsius and it...
2019-03-2903 minGenomics RevolutionSurvey of Genomes - Rickettsia prowazekiiWelcome to Genomics Revolution. This is Jake Lininger from the 2019 Hiram College Genetics course. Today we will look at Rickettsia prowazekii or R. prowazekii for short. During WWI, epidemic typhus infected around 30 million humans (1). R. prowazekii had been discovered previously as a causal agent for typhus, or typhus fever, but how it infects humans was yet to be discovered. In 1928, Dr. Charles Nicolle received a Nobel prize for discovering the vector for this infection; human body louse. Humans that develop typhus are infested with lice that carry R. prowazekii until skin is broken and the bacteria is a...
2019-03-2903 minGenomics RevolutionSurvey of Genomes - Thermoplasma acidophilumHello my name is Nikkia Schady and today I will be talking to you about the organism Thermoplasma Acidophilum. Thermoplasma Acidophilum is a thermoacidophilic archaeon. It is classified as being in the archaea domain due to its membrane lipid composition, evolutionary ribosomal RNA and conserved proteins. (2) Thermoplasma Acidophilum is also in the subgroup Euryarchaeota and it’s strain is Thermoplasma acidophilum DSM 1728. (3) From here on out I will just call it Thermoplasma as a short name.• This organism was first discovered when it was isolated from self-heated coal refuse piles. (1). It was first isolated by a man named Darl...
2019-03-2904 minGenomics RevolutionSurvey of Genomes - Methanococcus jannaschiiWelcome to Genomics Revolution. My name is Kritika Bhau and I am a student at Hiram College and currently taking the course called Genetics. I will be hosting the episode on the organism, Methanococcus jannaschii. It is also known as methanocaladococcus jannaschii. I will be calling the organism M. jannaschii throughout the podcast. M. jannaschii is important because it was also the first archaeon to have its complete genome sequenced. This sequence help identity many genes unique to domain Archaea. But, before we get into the genome analysis of this organism, let us talk more about M. Jannaschii to get som...
2019-03-2905 minGenomics RevolutionSurvey of Genomes - Entomopathogenic Xenorhabdus strainsWelcome to Genomics Revolution. This is Brad Goodner from the 2019 Hiram College Genetics course hosting this episode on the genomes of two strains of the genus Xenorhabdus - Xenorhabdus nematophila ATCC 19061 & Xenorhabdus bovienii SS-2004. I will call them X. nematophila and X. bovienii from here on out. These strains are in the Bacteria division gamma-Proteobacteria and within the family Enterobacteriaceae, meaning they are closely related to well known human gut symbionts and pathogens such as Escherichia coli and Salmonella enterica. However, Xenorhabdus strains are not found in the guts of humans, rather they are gut symbionts of particular soil nem...
2019-03-1105 minGenomics RevolutionIntroduction to a Survey of Genomes - Agrobacterium tumefaciens C58This is Brad Goodner. Welcome back to Genomics Revolution. In our first 6 episodes, we have introduced the terms genome and genomics, talked about how the field of genomics got its start, and looked at the steps of a genome project using the first ever sequenced cellular genome as an example.Now we will tour through a survey of some sequenced genomes. All three domains of life will be represented, but the Bacteria and Archaea will get the lion’s share. For each genome, we will learn why scientists are interested in the organism, some basic data about the ge...
2019-03-1008 minGenomics RevolutionThe First Cellular Genome - Part 2Welcome back to Genomics Revolution. I am Brad Goodner. Last time we were together, we walked through the strategy used by Craig Venter’s team at TIGR, The Institute for Genomics Research, to sequence the first genome of a cellular organism, Haemophilus influenzae strain Rd.Today, we will finish up our analysis of the July 1995 SCIENCE article by focusing on the biological implications of knowing the complete sequence of an organismal genome. The H. influenzae strain Rd genome is a single circular chromosome of 1,830,137 base pairs. Previous to this work, the sequence of 122 protein-coding genes and their su...
2019-01-0205 minGenomics RevolutionThe First Cellular Genome, Part 1Welcome back. I am Brad Goodner, Professor of Biology at Hiram College. We have reached the point of the first genome sequence from a cellular organism, published in July of 1995 in the journal SCIENCE.As we discussed earlier in Episode 4, Craig Venter and his colleagues at TIGR, The Institute for Genomics Research, came up with a probability-based approach, a shotgun approach, to sequencing a genome. Break it up into pieces and sequence enough pieces to cover the genome at least 5 times to hopefully obtain 99% of the genome sequence.The 1995 SCIENCE article by Robert Fleischmann and 39...
2019-01-0206 minGenomics RevolutionTwo Ways to Solve a Genomics Jigsaw PuzzleOnce the goal of obtaining a human genome sequence had been set by research scientists and several government agencies around the world, the big question was how to organize the effort. Any genome of a cellular organism, but especially the human genome, is a massive amount of information. How do you gather the information and how do you piece it all back together at the end? There was no technology available in the late 1980’s and early 1990’s, and there is still none to this day, that allows one to jump onto a giant DNA strand and determine its sequ...
2018-01-2206 minGenomics RevolutionThe Power of an Idea - The Human Genome ProjectWelcome back. I am Brad Goodner. While genetics as a scientific discipline did not need DNA sequences to get started, it certainly progressed at a much faster clip once one could see what genes actually looked like and determine how genes change due to different mutations. We discussed the Maxam-Gilbert chemical and Sanger enzymatic methods for sequencing DNA strands in our last episode. Their impact was so immediate that Gilbert and Sanger shared ½ of a Nobel Prize just a few short years later. Amazingly, it was Sanger’s second! The other ½ of that Nobel went to Paul Berg who led e...
2017-12-2907 minGenomics RevolutionGenetics Before GenomesI am Brad Goodner. Welcome back to Genomics Revolution. To fully understand the impact of having an organism’s complete DNA sequence, its genome, we need to put it into the proper context set by the previous 150 years. Genetics as an experimental science got its start in the middle of the 19th century with Mendel’s inheritance trials on pea plant phenotypes and with Meischer’s biochemical isolation of nuclein, what we now call DNA. Mendel’s ideas on the rules of inheritance in sexually reproducing eukaryotes was generalized into the concept of a gene as a definable unit of genet...
2017-12-2911 minGenomics RevolutionAn Introduction and Some DefinitionsWelcome to the podcast Genomics Revolution. I am Brad Goodner, Professor of Biology at Hiram College. This is a podcast about the biggest explosion in biological knowledge in human history and it is has been happening all around us over the last 30 years. The Genomics Revolution is based on 1) some basic knowledge about DNA – how it is structured and faithfully replicated, 2) an ambitious goal to fully understand the complete genetic basis of human biology, 3) new ways to store, collate, and compare incredibly large data sets, and 4) lots of determined biologists, chemists, computer scientists, and statisticians working together in new co...
2017-12-0905 min