Brandy Sargent - Podcast Details

Shows

Cell Culture Dish PodcastHow Real Time Titer Measurement And Monitoring Is Advancing Bioproduction Across Multiple ApplicationsThis panel discussion was originally published in the eBook “ Monoclonal Antibody Manufacturing Trends, Challenges, and Analytical Solutions to Eliminate Bioprocessing Bottlenecks” You can download all the articles in the series, by downloading the eBook. Panel discussion members: Carrie Mason - Associate Director, R&D at Lonza Biologics Laura Madia - Independent Industry Consultant Alan Opper – Director of HaLCon Sales at RedShiftBio David Sloan, PhD – Senior Vice President, Life Sciences at RedShiftBio Brandy Sargent, Editor in-chief, Cell Culture Dish and Downstream Column (Moderator) In this panel discussion, we talked with industry experts about antibody process development and manufacturing. Specifically focusing on current a...2024-02-2940 min

Cell Culture Dish PodcastBiomanufacturing Quality Management Systems – A holistic approachIn this podcast, we interviewed Katie Keller, Director of Quality and Safety at Asahi Kasei Bioprocess America, about the importance of quality management and how to achieve the best possible results. Topics included the most critical elements of quality management, how to ensure the purchase of high-quality equipment, and future trends. I started the conversation by asking Katie what she thought were the most critical elements of quality management. Katie replied by saying that a holistic approach to quality is best for any organization. It used to be that the quality unit was considered responsible for product quality, making all...2022-12-0510 min

Cell Culture Dish PodcastFlexible single-use mixers are key to achieving a next level mixing experience and reducing delivery lead timesIn this podcast we spoke with Klaus Kienle, Global Product Manager for the Mixing portfolio at Pall Corporation about the latest mixing technologies including single-use solutions, the need for increased flexibility, and a more standard vendor agnostic approach. The Role of Mixing in Biomanufacturing I started the conversation by asking Klaus if he could talk about the role that mixing plays in biomanufacturing and current challenges in this area. Klaus explained that mixing is an omnipresent process. It starts with upstream buffer media and ends in fill and finish. It is an important part of manufacturing across several modalities, including...2022-11-2116 min

Cell Culture Dish PodcastManufacturing high concentration mAbs challenges and solutionsIn this podcast, we spoke to Tom Watson, Group Leader, Product Management – Biotech Division, Gregor Kalinowski, Manager SLS Purification Consultants Europe, and Aude Iwaniec, R&D Bioprocessing Team Leader, all from Pall Corporation, about why high concentration mAbs are an increasingly important part of the biotech landscape, current manufacturing challenges and solutions, and future trends. High concentration drugs offer benefits for patients I began the discussion by asking why high concentration mAbs are an important topic in today’s biotech landscape. Tom explained that high concentration drugs are an important innovation because when a biotech drug can be prepared at high...2022-11-1415 min

Cell Culture Dish PodcastA Guide for Navigating the Single Use Technology Supply ChainIn this podcast we spoke with Derrick Alig, North American Western Regional Sales Manager for PSG Dover Biotech, Chris Couper, President and Founder of Liquidyne Process Technologies, and Phil Sanders Biotech Chief Innovation Officer at Agilitech about current supply chain challenges, possible solutions, what the future holds, and ways to navigate supply chain shortages to ensure manufacturers meet their timelines. Supply Chain Shortages I began the discussion by asking our panel members if they could discuss challenges that their customers are currently having with sourcing single use consumables and technologies. Derek began by discussing the lack of raw materials to...2022-11-0734 min

Cell Culture Dish PodcastUpstream and Downstream Processes – A Holistic ViewThis article was originally published in the eBook “ Future proofing Bioprocessing from Upstream to Downstream”. You can download all the articles in the series, by downloading the eBook. Not everyone has the luxury of building from the ground up. How do you create a unified system between upstream and downstream with existing equipment and processes? And if you have the ability to build from the ground up, how can you ensure that your design is future proof? We spoke with Phil Sanders, Biotech Chief Innovation Officer from Agilitech, to develop a set of best practices for creating a holistic process from...2022-10-1214 min

The Downstream Column PodcastNew online Gene Therapy Academy offers great resources to support scaling up from lab to production scaleIn this podcast, we spoke with Emanuel Krobath, Biopurification Specialist and Chiara Pacini, Bioprocess Specialist both with Pall Corporation about gene therapy process development including challenges and resources that are available for support. I began the discussion by asking Emanuel and Chiara to tell listeners a little bit more about their jobs and how they support gene therapy developers on the bench. Emanuel started by saying that as a bioprocess product specialist, he performs customer bench case studies at the customer site, specifically for the downstream process including vaccines, recombinant proteins, monoclonal antibodies and gene therapy products. He shared that th...2022-10-0513 min

The Downstream Column PodcastA Guide for Navigating the Single Use Technology Supply ChainIn this podcast we spoke with Derrick Alig, North American Western Regional Sales Manager for PSG Dover Biotech, Chris Couper, President and Founder of Liquidyne Process Technologies, and Phil Sanders Biotech Chief Innovation Officer at Agilitech about current supply chain challenges, possible solutions, what the future holds, and ways to navigate supply chain shortages to ensure manufacturers meet their timelines. Supply Chain Shortages I began the discussion by asking our panel members if they could discuss challenges that their customers are currently having with sourcing single use consumables and technologies. Derek began by discussing the lack of raw materials to...2022-09-2034 min

The Downstream Column PodcastManufacturing high concentration mAbs challenges and solutionsIn this podcast, we spoke to Tom Watson, Group Leader, Product Management – Biotech Division, Gregor Kalinowski, Manager SLS Purification Consultants Europe, and Aude Iwaniec, R&D Bioprocessing Team Leader, all from Pall Corporation, about why high concentration mAbs are an increasingly important part of the biotech landscape, current manufacturing challenges and solutions, and future trends. High concentration drugs offer benefits for patients I began the discussion by asking why high concentration mAbs are an important topic in today’s biotech landscape. Tom explained that high concentration drugs are an important innovation because when a biotech drug can be prepared at high...2022-08-2515 min

The Downstream Column PodcastBiomanufacturing Quality Management Systems – A holistic approachIn this podcast, we interviewed Katie Keller, Director of Quality and Safety at Asahi Kasei Bioprocess America, about the importance of quality management and how to achieve the best possible results. Topics included the most critical elements of quality management, how to ensure the purchase of high-quality equipment, and future trends. I started the conversation by asking Katie what she thought were the most critical elements of quality management. Katie replied by saying that a holistic approach to quality is best for any organization. It used to be that the quality unit was considered responsible for product quality, making all...2022-08-1100 min

The Downstream Column PodcastFlexible single-use mixers are key to achieving a next level mixing experience and reducing delivery lead timesIn this podcast we spoke with Klaus Kienle, Global Product Manager for the Mixing portfolio at Pall Corporation about the latest mixing technologies including single-use solutions, the need for increased flexibility, and a more standard vendor agnostic approach. The Role of Mixing in Biomanufacturing I started the conversation by asking Klaus if he could talk about the role that mixing plays in biomanufacturing and current challenges in this area. Klaus explained that mixing is an omnipresent process. It starts with upstream buffer media and ends in fill and finish. It is an important part of manufacturing across several modalities, including...2022-07-2716 min

Cell Culture Dish PodcastAccelerating cell and gene therapy development using custom media solutions early in process developmentIn this podcast, we spoke with Dr. Brad Taylor from Nucleus Biologics and Randy Jacinto from Cytiva about the benefits of using a custom media for cell and gene therapy process development. Topics included current development challenges, increases in the number of cell and gene therapies in clinical trials, and employing custom cell culture media solutions to address these issues. We started the discussion by talking about the most pressing challenges facing cell and gene therapy development. Randy began by explaining that the most pressing challenges are the cost of manufacturing, lower than expected adoption rates, and complex logistics around...2022-07-2121 min

The Downstream Column PodcastOptimizing Process Development Early – A critical factor in successful viral vector scale up and large scale manufactureIn this podcast, I spoke with John Ketz and Denis Kole about viral vector production, including current manufacturing challenges, navigating the road to commercialization, and successful scale up strategies. We began the interview by discussing the strides made in cell and gene therapy. Denis shared information about the several approved therapies and the more than 2,000 ongoing clinical trials. Denis added that while the immense potential of these personalized therapies is becoming more and more clear, the challenges and bottlenecks surrounding their development and manufacturing are also becoming a reality. This is especially true when it comes to producing and delivering s...2022-07-1213 min

Cell Culture Dish PodcastOptimizing Process Development Early – A critical factor in successful viral vector scale up and large scale manufactureIn this podcast, I spoke with John Ketz and Denis Kole about viral vector production, including current manufacturing challenges, navigating the road to commercialization, and successful scale up strategies. We began the interview by discussing the strides made in cell and gene therapy. Denis shared information about the several approved therapies and the more than 2,000 ongoing clinical trials. Denis added that while the immense potential of these personalized therapies is becoming more and more clear, the challenges and bottlenecks surrounding their development and manufacturing are also becoming a reality. This is especially true when it comes to producing and delivering s...2022-07-1100 min

Cell Culture Dish PodcastUsing End-to-End Integrated Solutions to meet the High Demand for Viral Vector GMP ManufacturingIn this podcast, I spoke with Hanna Lesch Ph.D., Chief Technology Officer at Exothera about viral vector manufacturing. We discussed current industry needs and challenges, scalability, end to end solutions, and key insights to a successful manufacturing and approval process. I began the discussion by asking Hanna about the viral vector manufacturing that she is working on at Exothera. She explained that Exothera is a Belgium-based CDMO delivering customized process development and GMP manufacturing services for gene therapy and viral vector-based vaccines. In 18 months, they built their new facilities with a GMP qualified manufacturing area of 2100 square meters. They h...2022-06-1406 min

The Downstream Column PodcastSingle-use Mixers – Ensuring the Customization, Scalability and Supply Required for SuccessIn this podcast, we talked with Dennis Hodgson and Phil Sanders from Agilitech about the benefits of single-use mixers, dealing with supply chain concerns, ensuring scalability, and tailoring a mixer to meet specific process needs. Benefits of Single-use Mixers We began the podcast by talking about the overall benefits of single-use technologies for mixing. Dennis explained that single-use mixers are very versatile and can be used to replace stainless steel vessels within the manufacturing area. Single-use mixers all have the same advantages of other single use components, such as coming fully sterile and eliminating the need to steam and clean...2022-05-2510 min

The Downstream Column PodcastIncreasing Liquid Chromatography Efficiency with Fully-controlled Binary BlendingCory Hinz talks about the different methods that are available for liquid chromatography mobile phase solutions and the benefits of inline blending. Cory also describes how to implement binary blending feeding of a liquid chromatography process using inline blending.2022-05-1907 min

Cell Culture Dish PodcastImplementing Quality by Design for AAV ProductsIn this podcast, we talked with Parth Trivedi, Business Development Manager, Pall Corporation, about the importance of implementing a Quality by Design strategy for AAV product manufacturing and specific key steps for successful assessment. We began by talking about the importance of Quality by Design (QbD) in AAV product manufacture and how this pertains to the regulatory landscape. Parth explained that there have been several recent regulatory approvals of gene therapy products, but in 2020 there were also regulatory setbacks. These setbacks mostly involved lack of sufficient data in the chemistry, manufacturing, and controls documentation or CMC. This led Pall to...2022-05-1223 min

Cell Culture Dish PodcastStreamline the ADC Path to the Clinic with One-stop Drug Development and Critical Technology AdvancementsIn this podcast, I talked with Dr. Jimmy Li, CEO of WuXi XDC, a WuXi Biologics subsidiary. We discussed the reasons for the formation of WuXi XDC, which was established via a joint venture between WuXi Biologics and WuXi STA, a WuXi AppTec subsidiary, and how one-stop drug development organizations greatly streamline a pathway to the clinic. He also shares new technologies available to make the development of Antibody Drug Conjugates (ADCs) more efficient and effective.2021-12-2010 min

Cell Culture Dish PodcastThe benefits of using a single-source for discovery, development and GMP manufacture of antibody drug conjugates and other novel bioconjugatesIn this podcast, I talked with Dr. Jimmy Li, CEO of WuXi XDC, a WuXi Biologics subsidiary. We discussed the reasons for the formation of WuXi XDC, which was established via a joint venture between WuXi Biologics and WuXi STA, a WuXi AppTec subsidiary, and how this company provides a true single-source for the discovery, development, and GMP manufacture of antibody drug conjugates and other novel bioconjugates with their highly efficient one-stop drug development platform.2021-12-1512 min

Cell Culture Dish PodcastIncreasing speed and efficiency of biotherapeutic drug development with stable poolsIn this podcast, we talked with Dr. Alison Porter, Head of Expression System Sciences, Lonza, about the use of stable pool expression to reduce drug development timelines. Highlights included the implementation of stable pools in current workflows, expected titers, and cutting-edge applications of the technology.2021-12-0118 min

Cell Culture Dish PodcastAddressing the increasing demand for single-use technologies and supply chain shortages with future proof systemsPhil Sanders talks about the increase in demand for single-use equipment and consumables and how this has led to supply chain shortages.2021-11-1916 min

The Downstream Column PodcastAddressing the increasing demand for single-use technologies and supply chain shortages with future proof systemsPhil Sanders talks about the increase in demand for single-use equipment and consumables and how this has led to supply chain shortages.2021-10-2816 min

Cell Culture Dish PodcastImplementing Adherent Cell Culture Technology for Cell Therapy BioprocessSeveral of the most promising candidates in the pipeline use mesenchymal stromal cells and pluripotent stem cells, both of which require an adherent substrate for native biological function. Thus, utilizing an adherent platform for production of these cell types provides several advantages, including shorter process development and optimization time, no need to adapt cells to suspension, and the ability to implement various surface modifications that promote the relevant biology.2021-09-2328 min

Cell Culture Dish PodcastEvolving Beyond the Status Quo with Next Generation Buffer Prep SolutionsIn this podcast, we spoke with Chris Rombach, Vice President of Sales and Marketing at Asahi Kasei Bioprocess America about buffer prep and delivery systems. We discussed current pain points and how next generation buffer prep solutions can greatly improve upon the status quo, including increasing the use of automation and remote operation, while reducing the overall footprint, labor and cost associated with more traditional approaches.2021-09-0814 min

The Downstream Column PodcastEvolving Beyond the Status Quo with Next Generation Buffer Prep SolutionsIn this podcast, we spoke with Chris Rombach, Vice President of Sales and Marketing at Asahi Kasei Bioprocess America about buffer prep and delivery systems. We discussed current pain points and how next generation buffer prep solutions can greatly improve upon the status quo, including increasing the use of automation and remote operation, while reducing the overall footprint, labor and cost associated with more traditional approaches.2021-08-1714 min

The Downstream Column PodcastIntegrated Freezing Solutions to Minimize Risk and Preserve Product QualityClaire Jarmey-Swan, Global Product Manager, Pall Corporation talks about the evolution freeze thaw technologies and how these new methods can streamline the process, minimize loss and maintain the highest product quality.2021-04-2710 min

Cell Culture Dish PodcastIntegrated Freezing Solutions to Minimize Risk and Preserve Product QualityClaire Jarmey-Swan, Global Product Manager, Pall Corporation talks about the evolution freeze thaw technologies and how these new methods can streamline the process, minimize loss and maintain the highest product quality.2021-04-2710 min

Cell Culture Dish PodcastEnabling process development for continuous bioprocessing: Answering the call for small scale tools that are fit for purposeThe priority of speed to market is often at odds with issues around development resources, facility space, and infrastructure for both development and manufacturing. Continuous bioprocessing provides solutions for many of these challenges in certain applications, but to deliver on this promise we need fit-for-purpose tools and technologies to enable process development and provide reliable transfer to commercial manufacturing.2021-04-0632 min

Cell Culture Dish PodcastThe CHO Cell Line – From Reliable Workhorse to State-of-the-art Protein PowerhouseAlan Dickson talks about the evolution of the CHO Cell line from isolation to workhorse of the biomanufacturing industry, to gene edited knockout variants. It is an interesting look at why CHO cells have been so successful and how this success continues to be improved upon for manufacture of emerging therapeutics.2021-03-0929 min

Cell Culture Dish PodcastThe Secret Ingredient in Your Gene Therapy Success RecipeIn this podcast, Ratish Krishnan, Associate Director for Cell & Gene Therapy BioProcessing for the Americas at MilliporeSigma about the importance of 3D culture and the challenges associated with growing and maintaining culture in 3D. We also discussed using a 3D culture bioreactor system, which employs clinostat technology to culture spheroids and organoids in a way that maintains the structure and function of in vivo cells.2021-02-1119 min

Cell Culture Dish Podcast3D cell culture bioreactor system for growing and maintaining spheroids and organoids with the structure and function of in vivo cellsIn this podcast, we spoke with Dr. Stephen Fey, Chief Research Officer and Co-founder, CelVivo about the importance of 3D culture and the challenges associated with growing and maintaining culture in 3D. We also discussed using a 3D culture bioreactor system, which employs clinostat technology to culture spheroids and organoids in a way that maintains the structure and function of in vivo cells.2021-02-0322 min

Cell Culture Dish PodcastApplications and Impact of CRISPR/CAS9 in BioprocessingExperts from Wuxi Biologics talk about one of the great technological advancements of the last decade, CRISPR/Cas9 technology. We discussed the technology’s potential and in particular its possibilities in the discovery and development of biopharmaceuticals. We also conducted a deep dive on its potential impact on bioprocessing and biomanufacturing.2020-12-2920 min

Cell Culture Dish PodcastOptimizing Protein Expression and Purification using Mass Spectrometry AnalysisIn this podcast, we conducted a panel discussion with experts from Peak Proteins on common protein challenges and the use of mass spec to overcome expression and purification issues. We also discussed how mass spec can also be utilized in working with complex proteins and in-process modifications.2020-12-1731 min

Cell Culture Dish PodcastNew Lab Set Up – Best Practices for a Successful StartIn this podcast, we spoke with Dr. Ann Rossi Bilodeau, Senior Bioprocess Applications Scientist and Dr. Catherine Siler, Field Applications Scientist both with Corning Life Sciences, who shared insightful tips for setting up a new lab. We discussed how to create a lab plan, maximize lab space, stay within budget and timelines. They also shared their experience in implementing lab safety and training as part of the new lab launch.2020-11-1826 min

Cell Culture Dish PodcastDemystifying the FBS Selection Process – A guide for evaluating product quality, origination and cost considerationIn this podcast, we talked with Chris Scanlon, Global Marketing Development Manager at Thermo Fisher Scientific about how to effectively evaluate which FBS product is right for each application. This includes weighing product quality levels and country of origin. Chris also shares strategies for maximizing purchasing options and new FBS products on the horizon.2020-10-1318 min

Cell Culture Dish PodcastReducing Fill Risk in Drug Product Manufacture Utilizing New State-of-the-Art Systems and PlatformsIn this podcast we talked with DQ Wang, PhD, and Vice President, Formulation, Fill and Finish of WuXi Biologics about their DP4 multi-product fill & finish facility featuring the Vanrx SA25 robotic, gloveless, isolator-based filling system. The system significantly reduces drug product fill risk and provides greater aseptic assurance.2020-09-1617 min

Cell Culture Dish PodcastThe Challenge of Staying Current with Regulatory Changes – How one company is providing a solutionIn this podcast, we talked with Ken Chen, MBA, Senior Director, Regulatory Affairs, WuXi Biologics about staying current with regulatory changes. We discussed how WuXi Biologics recently began publishing a quarterly summary of regulatory updates on new or revised guidance documents from the various global regulatory agencies and how this is a valuable resource for anyone in the biological drug development arena.2020-09-1010 min

Cell Culture Dish PodcastComputer Aided Biology Platform Helps Companies Meet the Challenges of 21st Century BiomanufacturingIn this podcast, we interviewed Markus Gershater, Chief Scientific Officer with Synthace about computer-aided biology and how it addresses several common biomanufacturing challenges. We also discussed ways to build a common culture between science and software.2020-09-0826 min

Cell Culture Dish PodcastBalancing Risk, Cost and Speed During Clinical Development While Still Maintaining QualityIn this podcast, we talked with Thierry Cournez Vice President, BioReliance® End-to-End Solutions, MilliporeSigma. We discussed effective ways for emerging biotechs to collect material quickly and cost-effectively for pre-clinical and clinical studies. We also discussed managing the need to move quickly with cost and quality.2020-07-2711 min

Cell Culture Dish PodcastSolving the challenges of standardizing cell counting to ensure reproducibility in experiments, assays and manufacturing processesIn this podcast we talked with Christian Berg, Global Product Manager at Chemometec about the importance of standardizing cell counting because while often overlooked, it is essential for reproducibility in experiments, assays and manufacturing processes. Cell counting is a challenging technique, with many pitfalls, that can delay entire projects. We discussed how new technologies are solving these challenges and enabling standardization of cell counting across organizations.2020-07-0717 min

Cell Culture Dish PodcastA Look Towards the Future of 3D Cell Culture – A panel discussionThis article is the fourth in a series that was published in the eBook “3D Model Systems: Spheroids, Organoids and Tissue Models”. You can download all the articles in the series, by downloading the eBook. Introduction and Overview Debbie King Researchers have used 2D cell culture since the early 1900s, but we know that growing cells on planar surfaces have some drawbacks. Cells grown in vitro in 2D space don’t behave like cells found in vivo. They lack critical cell-cell and cell-matrix interactions that drive their form, function and response to external stimuli. This limits their prognostic capabilities. More recentl...2020-07-0120 min

Cell Culture Dish PodcastMultistem – A Stable, Off-the Shelf Regenerative Medicine Has Demonstrated Promise in Treating Patients with Acute Respiratory Distress Syndrome or ARDSIn this podcast, we talked with Dr. Gil Van Bokkelen, Chairman and CEO, Athersys about recent clinical breakthroughs in regenerative medicine and manufacturing challenges. We also discussed Multistem and how it has been demonstrated to help patients with ARDS (Acute Respiratory Distress Syndrome).2020-06-0432 min

Cell Culture Dish PodcastBi-Specific Antibodies – The development, manufacture and promise of these cutting-edge therapeuticsIn this podcast, we conducted a panel discussion with experts from Selexis and KBI Biopharma on bi-specific antibodies. We examined bi-specific antibody development and manufacturing, including current challenges and key solutions. We also discussed the promise of these cutting-edge therapeutics and their future in medicine.2020-05-2732 min

Cell Culture Dish PodcastRapid At-line Media Analyzer Speeds Process Development by Eliminating Analytics BottleneckIn this podcast, we talked with Dr. Glenn Harris, Director of Integrated Life Sciences Platforms at 908 Devices, about the benefits and challenges of implementing rapid media analysis in process development, including the bottleneck created by outsourcing samples to core labs. We also discussed an easy to implement, benchtop media analyzer that permits comprehensive media analysis in real-time, thus speeding process development efforts.2020-05-1416 min

Cell Culture Dish PodcastNon-Animal Origin cell culture supplements and manufacturing aids for biologics manufacturingIn this podcast, we talked with Dr. Tobias Hertzig, Regulatory Affairs Manager and Dr. Ulrich Tillmann, Global Product Manager, Supplements and Manufacturing Aids both representing Merck KGaA, Darmstadt, Germany. We discussed non-animal origin cell culture media supplements, including regulatory advantages, performance metrics and their use in biologics manufacturing.2020-05-1122 min

Cell Culture Dish PodcastMesenchymal Stem Cell Culture – Challenges and solutions for isolation, expansion and maintenanceIn this podcast and accompanying article, we talked with Dr. Jennifer Chain, Scientific Director of Research and Development, Oklahoma Blood Institute about isolation of mesenchymal stromal cells from cadaveric bone marrow and the differences between live and cadaveric donors. We also discussed the expansion and maintenance of mesenchymal stromal cells in culture including media design and selection.2020-03-2518 min

Cell Culture Dish PodcastCryoVault Freeze and Thaw Platform Provides a Scalable, Robust and Single-Use End-to-End Solution for Bulk Drug SubstanceIn this podcast, I talked with Max Blomberg, Executive Director of Operations and Andrew Govea, Senior Product Engineer, at Meissner about the challenges of handling bulk drug substance, specifically freeze and thaw and how the need for scalability, flexibility and a robust approach led to the development of CryoVault™. CryoVault™ offers a unique and intelligently designed end-to-end freeze and thaw process solution.2020-02-1126 min

Cell Culture Dish PodcastGenerating actionable data and analysis in complex models using live-cell analysisIn this podcast and accompanying article, we talked with Paul Jantzen, Product Manager for IncuCyte at Sartorius about the benefits of real-time live-cell analysis and image processing workflows. We also discussed how live-cell analysis is enabling the use of neuronal cell models to study cell health, morphology, function and cell dynamics.2019-12-1214 min

Cell Culture Dish PodcastImproved gene therapy analytics permit monitoring of critical quality attributes and increased manufacturing efficiencyIn this podcast and accompanying article, we interviewed Jonathan Royce, Director, Instruments Business Unit, Vironova about analytics in gene therapy manufacturing. Specifically Jonathan details new technologies to monitor critical quality attributes, reduce development time and increase the number of projects that can be run.2019-12-1108 min

Cell Culture Dish PodcastNew Advancements in Cell and Gene Therapy Implementing Precision MedicineIn this podcast and accompanying article, we talked with Dr. Paul Wotton, Chief Executive Officer, Obsidian Therapeutics about the evolution of cancer therapy and new advancements in the space including precision medicine. Our discussion included Obsidian's cytoDRiVE™ platform that provides a technology, in which the level and timing of protein activity are fully controlled in a dose-dependent manner by an FDA-approved small molecule.2019-12-1030 min

Cell Culture Dish PodcastLive Cell Analysis for Neuroscience ResearchIn this podcast, we talk with Susana Alcantara, Senior R&D Scientist and Neuroscience Program Leader, BioAnalytics, Sartorius about the ways real-time live cell analysis is enabling neuroscience research. We also discussed how real-time cell analysis can be used in neurological disease model development and in studying disease pathology to enable drug discovery. Show Notes: I began the interview by asking Susana what she saw as the biggest challenges surrounding neuroscience research as it relates to cell monitoring and analysis. She explained that she saw the main challenges as being intrinsic to the type of research, specifically that the cells...2019-10-2911 min

Cell Culture Dish PodcastUsing 3D cell-based human liver microtissue models in predicting adverse effects caused by chronic exposure to engineered nanomaterialIn this podcast and accompanying article, we interviewed Dr. Shareen Doak, Professor of Genotoxicology and Cancer at Swansea University Medical School and Dr. Wolfgang Moritz, Co-founder and Head of External Collaborations and IP, InSphero about the use of 3D cell-based human liver microtissue models to predict the adverse effects of chronic exposure to nanomaterial. We also discussed the use of liver microtissue models for drug discovery and development.2019-10-1316 min

Cell Culture Dish PodcastRaw Material Quality – Identifying and Mitigating VariationIn this podcast, we interviewed Cory Card, Principal Scientist Life Sciences, GE Healthcare about raw material quality, its impact on cell culture and technologies to identify and mitigate raw material variation.2019-09-2417 min

Cell Culture Dish PodcastAntibody Drug Conjugate (ADC) Development and Manufacturing Challenges and SolutionsIn this podcast, we interviewed Dr. J.J. Luo, Executive Director and Dr. Lily Yin, Head of Biologics Conjugation Development, at WuXi Biologics about the state of the ADC marketplace and the challenges in developing antibody drug conjugates. We then dove into reasons behind why they built a dedicated manufacturing facility for ADCs and other bioconjugates and how a single-source development platform can benefit bioconjugate drug development efforts.2019-09-0916 min

Cell Culture Dish PodcastMustang Bio’s gene therapy has tremendous potential to cure XSCID or Bubble Boy Disease based on study results from St. JudeIn this podcast, we interviewed Dr. Manny Litchman, President and CEO, Mustang Bio and Dr. Knut Niss, Chief Technology Officer, Mustang Bio about the exciting study results for their gene therapy candidate to treat X-SCID, why this disease is a good fit for gene therapy and next steps.2019-07-3114 min

Cell Culture Dish PodcastSuccessful Methods for Perfusion Process OptimizationIn this podcast, we interviewed Dr. Andreas Castan, Principal Scientist at GE Healthcare Life Sciences about the best methods for optimizing perfusion processes. This included a discussion of tools for media optimization and innovative cell separation techniques.2019-07-2416 min

Cell Culture Dish PodcastHow Live Cell Analysis Technology is Meeting the Needs of Ever Evolving Advanced Cell ModelsIn this podcast and accompanying article, we interviewed Dr. Kimberly Wicklund, Head of Product Management for the IncuCyte® at Sartorius about how live cell analysis is meeting the needs of advanced cell models and how the launch of the new IncuCyte SX1 is providing scientists more options when it comes to using live cell analysis in their workflows.2019-06-2616 min

Cell Culture Dish PodcastInnovative vaccine manufacturing enables the delivery of vaccines to the developing worldIn this podcast and accompanying article, we interviewed Dr. Alex Chatel, Product Manager, Viral Applications, Univercells about the biggest challenges facing vaccine manufacturing today, why it is so difficult to manufacture and deliver vaccines to the developing world and how a novel technology with the support of the Gates Foundation is poised to address these challenges.2019-06-1918 min

Cell Culture Dish PodcastOptimizing Fed Batch Culture – Developing New Tools and Methods to Improve ProductionIn this podcast and accompanying article, we interviewed Dr. Aline Zimmer, Head of R&D, Advanced Cell Culture Technologies at Merck about the importance of optimizing fed batch cell culture for successful biomanufacturing. Aline discussed how her team has developing new tools and methods for optimizing fed batch culture and the impact this has had on productivity and product quality.2019-05-2912 min

Cell Culture Dish PodcastUsing cell culture models of endocrine resistance to improve triple positive breast cancer treatment options.Gibco Cell Culture Hero, Dr. Hillary Stires discusses her work researching better treatment options for triple positive breast cancer patients. She explains how the use of cell culture modeling helps drive her research. She also talks about her experience as a cell culture hero and her passion for the scicomm movement on social media platforms.2019-04-0119 min

Cell Culture Dish PodcastIntegrating bioprocessing steps to improve efficiency and reduce costIn this podcast, we interviewed Dr. Rick Morris, Senior Vice President, R&D, Biotech, Pall Life Sciences, about integrating bioprocessing steps to improve efficiency, reduce cost and address current challenges in the industry. Specifically we looked at how upstream and downstream can be more integrated and how to increase integration in existing facilities.2019-02-2108 min

Cell Culture Dish PodcastExpediting cell and gene therapy workflowsIn this podcast and accompanying article, we interviewed Mr. Dave Eansor President of the Protein Sciences Segment at Bio-Techne and Dr. Sean Kevlahan, Senior Director of Cell and Gene Therapy at Bio-Techne about new technologies that can be implemented to expedite cell therapy and gene therapy workflows and facilitate the path from bench to clinic.2019-02-0626 min

Cell Culture Dish PodcastA Road Map for Efficient Fed-Batch Cell Culture OptimizationIn this podcast and accompanying article, we interviewed Dr. Andreas Castan, Principal Scientist at GE Healthcare Life Sciences about the most efficient ways to optimize fed-batch cell culture production for CHO-based antibodies. This included a discussion of media vs. feed strategies, incorporating critical quality attributes, evaluating the impact of optimizing on downstream processing and more.2019-02-0515 min

Cell Culture Dish PodcastViral Vector Manufacturing for Gene TherapyIn this podcast and accompanying article, we interviewed Dr. Mats Lundgren, PhD Customer Applications Director, Life Sciences, GE Healthcare, Sweden about developing platform processes for viral vector manufacturing and Mats describes a recently developed platform process for adenovirus production.2019-01-2316 min

Cell Culture Dish PodcastKey Considerations in Gene Therapy Manufacturing for Commercialization – A panel discussionPanel discussion that I moderated at this year’s Cell and Gene Therapy Bioprocessing and Commercialization Conference. The discussion covers the latest in gene therapy manufacturing strategies, analytical analysis, cost of goods and key regulatory considerations.2018-12-1033 min

Cell Culture Dish PodcastClosed System Cell Therapy Manufacturing – Moving from the lab to clinical and commercial productionPanel discussion on key considerations for moving cell therapies from lab to commercial manufacturing, including the benefits of manufacturing in a closed system. We also discussed what were some of the key hurdles and possible solutions associated with transitioning to clinical and commercial cell therapy manufacturing.2018-07-2624 min

The Downstream Column PodcastStart With The End In Mind. How to facilitate transfer from non-GMP to GMP ManufacturingAvril Vermunt, bioprocess hardware specialist at GE healthcare talks about transferring a process from non-GMP to GMP manufacturing, particularly how resource-intensive it is for process development and manufacturing groups. Avril has several years of experience working in the technology transfer group at a large pharmaceutical company. We discussed with her best practices and how being proactive and one step ahead can make the process more seamless.2018-06-2814 min

Cell Culture Dish PodcastStart With The End In Mind. How to facilitate transfer from non-GMP to GMPIn this podcast, we interviewed Avril Vermunt, bioprocess hardware specialist at GE healthcare about transferring a process from a non-GMP to a GMP environment, particularly how resource-intensive it is for process development and manufacturing groups. Avril has several years of experience working in the technology transfer group at a large pharmaceutical company. We discussed with … Continue reading Start With The End In Mind. How to facilitate transfer from non-GMP to GMP →2018-06-2600 min

Cell Culture Dish PodcastModeling Downstream Disruptive Technologies to Improve Downstream Bioprocessing.In this podcast, we interviewed Dr. Yuyi Shen about the benefits of modeling disruptive technologies to improve downstream bioprocessing and evaluating the financial impact of implementing these technologies. View show notes at downstreamcolumn.com/podcast/modeling-downstream-disruptive-technologies-to-improve-downstream-bioprocessing/2018-06-0800 min

The Downstream Column PodcastModeling Downstream Disruptive Technologies to Improve Downstream Bioprocessing.Dr. Yuyi Shen, Principal Scientist, Grifols, talks about the benefits of modeling downstream disruptive technologies to improve downstream bioprocessing and evaluating the financial impact of implementing these technologies.2018-06-0708 min

Cell Culture Dish PodcastSuccessful Mesenchymal Stem Cell Manufacturing for Cell Therapy ApplicationsDr. Ross Macdonald, CEO Cynata Therapeutics, talks about the use of mesenchymal stem cells for Cell Therapy applications and key criteria for successful mesenchymal stem cell manufacturing.2018-05-3129 min

The Downstream Column PodcastCell Harvesting – Selecting a technology compatible with cell density and feed turbiditySilke Bergheim-Pietza, Global Product Manager Depth Filtration, Pall. discusses challenges faced in the cell harvesting step, in particular, selecting the best cell harvesting method based on the cell density and feed turbidity of the process and meeting the demand of increasing cell densities. Download the cell harvesting technology selection guide and use the guide to compare the latest single-use harvesting technologies.2018-04-1011 min

Cell Culture Dish PodcastBioprocessing Technologies for Stem Cell TherapyRicardo Baptista, Lead Process Development Scientist, Cell and Gene Therapy Catapult, UK talks about his impressions on current bioprocessing technologies for stem Cell Therapy manufacturing and possible future innovations.2018-04-0908 min

Cell Culture Dish PodcastCell Therapy Manufacturing – Opportunities and ChallengesDr. Robert Preti talks about the past, present, and future of global Cell Therapy manufacturing. We also discussed major industry milestones, manufacturing challenges, and opportunities for further improvements.2018-03-2019 min

Cell Culture Dish PodcastContinuous Biomanufacturing Implementation Now and in The FutureIn this podcast, we interviewed Dr. Peter Levison, Senior Marketing Director – Downstream Processing, Pall Life Sciences about the evolution and benefits of continuous biomanufacturing, what implementation looks like today and what still needs to be realized for full industry adoption. View show notes at downstreamcolumn.com/podcast/continuous-biomanufacturing-implementation-now-and-in-the-future/2018-03-0700 min

The Downstream Column PodcastContinuous Biomanufacturing Implementation Now and in The FutureDr. Peter Levison, Senior Marketing Director – Downstream Processing, Pall Biotech talks about the evolution and benefits of continuous biomanufacturing, what implementation looks like today and what still needs to be realized for full industry adoption.2018-03-0612 min

Cell Culture Dish PodcastCell Harvesting – Selecting a technology compatible with cell density and feed turbidity on Cell Culture Dish.In this podcast and accompanying article, we interviewed Silke Bergheim-Pietza, Global Product Manager Depth Filtration, Pall. We discussed challenges faced in the cell harvesting step, in particular, selecting the best cell harvesting method based on the cell density and feed turbidity of the process and meeting the demand of increasing cell densities. Download the cell … Continue reading Cell Harvesting – Selecting a technology compatible with cell density and feed turbidity on Cell Culture Dish. →2018-02-0200 min

The Downstream Column PodcastAddressing Challenges in Downstream Biomanufacturing with a Platform Purification ApproachJonathan Royce, Business Leader, Chromatography Resins, GE Healthcare Life Sciences, talks about the biggest challenges in Downstream biomanufacturing including overcoming bottlenecks, changing antibody structures and bioburden control. Jonathan shared how a purification platform can address some of these issues and discusses purification challenges that still need to be resolved.2018-01-3116 min

Cell Culture Dish PodcastAddressing Challenges in Downstream Biomanufacturing with a Platform Purification ApproachIn this podcast, we interviewed Jonathan Royce, Business Leader, Chromatography Resins, GE Healthcare Life Sciences, about the biggest challenges in Downstream biomanufacturing including overcoming bottlenecks, changing antibody structures and bioburden control. Jonathan shared how a purification platform can address some of these issues and discusses purification challenges that still need to be resolved. view show … Continue reading Addressing Challenges in Downstream Biomanufacturing with a Platform Purification Approach →2018-01-3000 min

Cell Culture Dish PodcastNovel Technology Enables Encapsulated Cell Therapies Without Triggering FibrosisDr. Paul Wotton, CEO, talks about Sigilon Therapeutics’ discovery platform. The platform combines cell engineering and biocompatible Afibromer™ technology to enable cell therapeutics that do not trigger fibrosis.2017-12-1418 min

The Downstream Column PodcastProtein A Chromatography – A look at where we have been and where we are goingJonathan Royce, Business Leader, Chromatography Resins, GE Healthcare Life Sciences, talks about the evolution of Protein A including the latest developments in Protein A chromatography resins. We also discussed what the future holds for this purification mainstay and how it can continue to address the changing needs of biopharma.2017-12-1225 min

Cell Culture Dish PodcastProtein A Chromatography – A look at where we have been and where we are goingIn this podcast, we interviewed Jonathan Royce, Business Leader, Chromatography Resins, GE Healthcare Life Sciences, about the evolution of Protein A including the latest developments in Protein A chromatography resins. We also discussed what the future holds for this purification mainstay and how it can continue to address the changing needs of biopharma. View show … Continue reading Protein A Chromatography – A look at where we have been and where we are going →2017-12-0700 min

The Downstream Column PodcastResolving Large Scale Buffer Management ChallengesJoakim Lundvist, Modality Manager, BioProcess™ Hardware, GE Healthcare talks about large-scale buffer management challenges. Buffer preparation is known to be one of the most resource-intensive activities in biomanufacturing as large volumes of buffers and process liquids are often required. So how can this be done in a more efficient way? How can more capacity be added to buffer preparation without adding major capital investment? Are there ways to reduce the manufacturing footprint and time spent on buffers?2017-11-2911 min

Cell Culture Dish PodcastResolving Large Scale Buffer Management ChallengesIn this podcast, we interviewed Joakim Lundvist, Modality Manager, BioProcess™ Hardware, GE Healthcare about large-scale buffer management challenges. Buffer preparation is known to be one of the most resource-intensive activities in biomanufacturing as large volumes of buffers and process liquids are often required. We examined the biggest challenges in buffer management and explored how technologies … Continue reading Resolving Large Scale Buffer Management Challenges →2017-11-2900 min

Cell Culture Dish PodcastFBS – It’s Not All Created Equal – What Consumers Need to KnowAlyssa Master, Ph.D., Senior Manager of Science and Applications, Nucleus Biologics talks about some of the challenges of working with FBS, why not all FBS is created equal and suggestions for FBS customers who are trying to navigate these issues.2017-11-0914 min

Cell Culture Dish PodcastDo You Have to Sacrifice Antibody Titer for Quality? Employing cell biology to get the best of both worldsDr. Adam Elhofy, Ph.D., Chief Scientific Officer, Bio-Ess Laboratories, talks about the relationship between antibody titer and product quality attributes such as glycosylation. Dr. Elhofy shares his thoughts on both challenges and opportunities in optimizing for both high titer and glycosylation profiles.2017-10-2622 min

Cell Culture Dish PodcastImplementation of Single-use Bioreactors for Microbial Fermentation is Easier Than You ThinkKen Clapp, a senior member of GE Healthcare Life Sciences' upstream product management team explores in more detail the implementation of single-use systems for microbial fermentation including which processes make good candidates for single-use and considerations for transitioning from stainless steel.2017-10-1818 min

Cell Culture Dish PodcastSingle-use Bioreactors for Fermentation – It’s now a reality that deserves considerationKen Clapp, a senior member of GE Healthcare Life Sciences' upstream product management team discusses the use of single-use bioreactors for fermentation, how single-use technology has evolved to meet the more rigorous demands of fermentation, and the benefits of single-use for this application.2017-10-1212 min

Cell Culture Dish PodcastBioengineered Organ Implants Poised to Change Treatment Paradigms in Esophageal CancerJim McGorry, CEO and Saverio LaFrancesca, President and Chief Medical Officer talk about how Biostage is using Cell Therapy to improve the standard of care in adult and pediatric esophageal disease.2017-06-2215 min

Cell Culture Dish PodcastImproving lab productivity and reproducibility by taking a fresh look at existing workflows and protocolsSarah Simons, Automation Field Project Manager, Beckman Coulter Life Science talks about how to improve lab productivity and reproducibility by "spring cleaning" your methods and taking a fresh look at existing workflows and protocols.2017-05-2324 min

Cell Culture Dish PodcastSmart Cell Culture Monitoring – Transforming the way we look at cells in culturePhilip Mathuis, Co-founder and CEO, Ovizio, talks about cell culture monitoring, the latest technology for monitoring cells in culture without the need for sampling, and where monitoring could move in the future.2017-04-1317 min

Cell Culture Dish PodcastJob Outlook for the Biomanufacturing IndustryIn this podcast, we interviewed Hillary Kaplan, Director, Bioprocess CMC Development & Operations, Klein Hersh about the job outlook for the biomanufacturing industry from a recruiter’s perspective. Hillary partners with organizations ranging from VC backed start-ups and mid-size biotechs to large multinational biopharma companies in helping to identify individuals working exclusively in biologics/large molecule development. … Continue reading Job Outlook for the Biomanufacturing Industry →2017-04-0600 min

The Downstream Column PodcastJob Outlook for the Biomanufacturing Industry a Recruiter’s PerspectiveHillary Kaplan, Director, Bioprocess CMC Development & Operations, Klein Hersh talks about the job outlook for the biomanufacturing industry from a recruiter’s perspective.2017-04-0513 min

Cell Culture Dish PodcastEnabling Vaccine Manufacturing and Viral Vector Production using the iCELLis – a single-use, automated, and closed manufacturing platformIn this podcast, we were fortunate to have a group of experts for a panel discussion on vaccine manufacturing and viral vector production.2017-03-0728 min

The Downstream Column PodcastCell Culture Media Optimization – It’s about more than just high protein titerDr. Adam Elhofy, Ph.D., CSO, Essential Pharmaceuticals, talks about cell culture media optimization. We discussed the evolution of cell culture media optimization, current goals, and the latest strategies and tools being used.2016-07-1914 min

Cell Culture Dish PodcastCell Culture Media Optimization – It’s about more than just high protein titerIn this podcast, we interviewed Dr. Adam Elhofy, Ph.D., CSO, Essential Pharmaceuticals, about cell culture media optimization. We discussed the evolution of cell culture media optimization, current goals, and the latest strategies and tools being used.2016-07-1900 min

The Downstream Column PodcastAutomated Stem Cell Based High Throughput Drug Screening for ALS and Parkinson’sDr. Jan Bruder talks about his work at the Max Planck Institute for Molecular Biology in Münster Germany and his group’s use of automated high throughput drug screening program for ALS and Parkinson’s Disease.2016-06-1427 min

Cell Culture Dish PodcastAutomated Stem Cell Based High Throughput Drug Screening for ALS and Parkinson’s – Dr. Jan BruderIn this podcast, we interviewed Dr. Jan Bruder about his work at the Max Planck Institute for Molecular Biology in Münster Germany and his group’s use of automated high throughput drug screening program for ALS and Parkinson’s Disease.2016-06-0727 min