Shows
The Conversational Flow - An Official Podcast of KCASFlow Cytometry & Autoimmunity – A High Affinity Pharmacodynamic MatchIn episode 21 of “The Conversational Flow” podcast, our hosts, Adam and Brian, explore the transformative power of gene therapy, starting things off by highlighting a compelling case involving CRISPR with milestones that once seemed impossible. They dive into the science behind CRISPR’s precise gene-editing capabilities and its potential for rapid, customizable treatments targeting rare and complex genetic mutations. The conversation also touches on the broader landscape of gene therapy delivery mechanisms and how advances are gradually expanding what's possible for more patients. Shifting gears, they critically examine recent vaccine policy developments and express concern about anti-scientific sentiment influencing public...2025-06-1451 min
Flow StarsVirginia Litwin (International Society For Advancement of Cytometry)In this episode, Peter O'Toole is joined by Virginia Litwin, the President-Elect of the International Society For Advancement of Cytometry (ISAC).They discuss Virginia's journey from academia to industry and her pivotal role in the creation and development of CYTO Women, a committee that's having a genuine positive impact on the opportunities available for women in cytometry.She also shares her experiences in translational science, working closely with clinical trials, and her passion for standardization in flow cytometry. Watch or listen to all episodes of Flow Stars: flowstars.bitesizebio.com2025-05-3055 minThe Conversational Flow - An Official Podcast of KCASImpactful Developments Across Oncology and Regulatory ScienceIn this milestone 20th episode of The Conversational Flow, hosts Adam and Brian dive into impactful developments across oncology, regulatory science, and bioanalytical innovation. The episode highlig4 hts a major Keytruda study showing the first significant improvement in head and neck squamous cell carcinoma outcomes in over two decades, the FDA’s pilot integration of generative AI in scientific review processes, and Sanofi’s acquisition of AI-developed bispecific antibodies for autoimmune diseases. The conversation rounds out with thoughtful discussion on data harmonization across multicenter flow cytometry studies and updates on KCAS’s scientific contributions at CYTO and regional events.“The Conversational Flow”...2025-05-1453 minThe Conversational Flow - An Official Podcast of KCASBuilding on Backbone Panels: Standardization, Speed, and IntegrationIn the 19th episode of “The Conversational Flow”, our hosts, Brian and Adam, dive into the utility and innovation behind backbone panels in spectral flow cytometry, spotlighting a recent paper from BMS that presents a robust, flexible assay designed for immune monitoring using the Aurora platform. They discuss how backbone panels streamline assay development, enhance reproducibility, and are well-suited for integration with AI-driven gating strategies. The conversation also touches on industry trends shared at WRIB, including the push toward global harmonization of instruments and standards in clinical trials. With insights on panel optimization, autofluorescence extraction, and metadata standardization, the epis...2025-04-141h 03
Flow StarsSpectral Flow Cytometry SpecialLet's shift our focus from just the people behind the science to the technologies they're using.In this special episode, we dive deep into spectral flow cytometry with a panel of leading experts, featuring:• Tamar Tak, Coordinator Flow Cytometry Facility, Leiden University Medical Center• Domenico Lo Tartaro, University of Modena and Reggio Emilia• Andrew Patentreger, Senior Team Lead, Broad Institute of MIT and Harvard• Kelly Lundsten, Biotechnology Market and Technical Bioassay Consultant, Luminous Bioanalytical Consulting• John Bianchi Colangeli, Resource Technologist I, Broad Institute of MIT and HarvardThey introduce the fundam...2025-03-211h 06
Royal Academy of Intl. Flow Cytometry AffairsChatting Around A Whole New Dumpster Fire | The ChUG PodcastAs much as I want to keep the ChUG podcast light and useful, we've entered a time where every piece of news we hear from the current administration is highly concerning. The media is discussing the stock market using stock images of people grabbing their face with their hands, we hear of NIH cuts and layoffs, and uncertainty is the only variable that is constant. I was happy to have Camille Stegen, Kathy Kluepfel, and Daniel Vocelle to discuss and provide some sense of clarity on how to move forward. So this is not as fun a discussion as...2025-03-191h 24Royal Academy of Intl. Flow Cytometry AffairsBuy or Lease with First American (with Andrew Card and Nick Borkowski) | Tools of the TradeTo Buy or to Lease? Finding funds for the purchase of flow cytometry equipment in your core facility can be challenging. In this first installment of the Tools of the Trade podcast, we discuss the leasing option offered by First American with Andrew Card and Nick Borkowski. RAIFCA's Tools of the Trade podcast highlights lesser-known tools available to flow cytometry core facility staff and users. We do not necessarily endorse the products discussed; our goal is to help listeners gain a better understanding of them.2025-03-1955 minThe Conversational Flow - An Official Podcast of KCASFlow Cytometry & Cell Therapy: One Cell Can Make a World of DifferenceIn this engaging episode of the podcast, hosts Brian Wile and Adam Cotty welcome special guests Michael Naso, Hillary Quinn, and Barry Morse from The Therapeutic Innovations Group—a powerhouse team of scientists and biotech veterans with decades of experience across biologics, small molecules, and cell therapy. The conversation dives deep into the guests’ unique career journeys, and their own consulting venture aimed at helping startups and academic teams navigate early-stage biotech development. Along the way, listeners are treated to insider stories, genuine camaraderie, and valuable insights into cutting-edge topics like CAR-T cell analysis, flow cytometry, and the evolving land...2025-03-141h 08The Conversational Flow - An Official Podcast of KCASFlow Validations and Guidance Document Changes in 2024-2025Episode 17 of The Conversational Flow podcast, hosted by Brian Wile and Adam Cotty, dives into the evolving landscape of flow cytometry networking and regulatory guidance, where they analyze recent FDA biomarker validation guidance, comparing it to existing industry standards like CLSI H62. Our hosts explore the challenges of harmonizing flow cytometry reporting, the role of regulatory clarity, and the potential for standardized data formats to drive the field forward. Packed with insights on professional networking and scientific validation, this most recent episode offers a deep dive into both career development and regulatory trends in flow cytometry.“The Co...2025-02-1445 minThe Conversational Flow - An Official Podcast of KCASThe Role of Flow in Drug Autoimmune Indication ResearchIn episode 16 of The Conversational Flow podcast, hosts Brian Wile and Adam Cotty are joined by Allie Greenplate and Ayman Rezk to explore “The Role of Flow in Drug Autoimmune Indication Research.” The discussion delves into the surge in pharmaceutical R&D for immune and autoimmune indications, highlighting the influence of oncology treatments in shaping this trend. The guests shed light on how advancements in high-dimensional flow cytometry, particularly with more than 20-color capabilities, have accelerated breakthroughs in this field. They also discuss the cutting-edge frontiers of autoimmune research, from innovative therapies to emerging technologies in development. This episode prov...2025-01-141h 00
Bloody MindedEpisode 28 - In the Lab: Flow Cytometry, Mass Spectrometry & Cytogenetics in MMCan’t get enough of obscure haematological lab chat, can you? Well we’ve got good news for you! Join us today as we delve even deeper into the arcane depths of flow cytometry, mass spectrometry and cytogenetics as they apply to myeloma. We promise we didn’t just make these words up.02:23 - Flow cytometry: overview04:01 - The flow cytometer: practical aspects09:01 - How we interpret flow11:44 - Myeloma flow markers14:32 - Quality in flow21:26 - Mass spectrometry25:59 - Cytogenetics27:46 - Cytogenetics assay principles30:30 - Karyotype abnormalities31:44 - FISH...2024-12-1742 minThe Conversational Flow - An Official Podcast of KCASFantastic Fluorophores and Where to Find Them (w/ Kelly Lundsten)In this exciting 15th episode of The Conversational Flow podcast, titled "Fantastic Fluorophores and Where to Find Them," hosts Brian Wile and Adam Cotty from KCAS Bio are joined by special guest Kelly Lundsten from FluoroFinder. Together, they dive into the fascinating world of spectral flow cytometry, exploring key questions such as whether spectra or brightness is more critical for controls, which traditional fluorophores the field should consider moving away from, and what potential pitfalls new dyes might present for users. They’ll also discuss the operational benefits of adopting newer dyes, including how they can simplify cocktail preparation an...2024-12-141h 05
That's What I Call Science!Episode 263: The Australasian Cytometry SocietyJoin us for this week’s episode, in which Olly and Georgia make their way through the poster session at the Australasian Cytometry Society’s annual 2024 conference! Cytometry may not be a word you’ve heard before, but is all about cells. And, by studying the cells in our bodies, the researchers in today’s episode have been making leaps and bounds in medical research in order to help the patients that need them.Show theme music: Kevin MacLeodHost: Olly Dove (@olly_dove)Co-Host: Georgia Stewart (@ggstew25)Prod...2024-11-2427 minThe Conversational Flow - An Official Podcast of KCASModifying Flow Cytometry Panels: the “Backbone” of Flow CytometryA flow cytometry backbone panel is a set of antibodies that can be used as a starting point for identifying cell types, and then supplemented with additional markers. On the most recent episode of “The Conversational Flow”, our hosts, Brian and Adam, spend some time together as they answer the questions of “What is a backbone panel?”, “What type of changes should be considered for a backbone panel?”, “What are the advantages to backbone panels for translational teams?”, “What should one be careful of when modifying a backbone panel?”, “Should you use a backbone panel for a translational research program?”, and many ot...2024-11-1450 minRoyal Academy of Intl. Flow Cytometry AffairsChUG podcast #21 - Meet Locally, Flow GloballyRegional flow cytometry are where it's at. This is what we have decided here at ChUG. We invited Aja Rieger to discuss the CoLD Summit, Karla Gorena to talk about FlowTex, and Daniel Vocelle to cover GLIIFCA. If you have unquestionably attended CYTO without any second look at other meetings, you've been missing out and this podcast is for you!Please note: David got pretty excited and dropped some expletives in the first minute of the podcast. Then he got a hold on himself. Edit 11/13/24: Karla Moncada Gorena is now Director of Business Development at KCAS! C...2024-11-131h 17The Conversational Flow - An Official Podcast of KCASLow Content Analysis: When a smaller panel is better than a larger oneDuring the most recent episode of “The Conversational Flow” podcast, our hosts Brian and Adam talk about those times when a smaller panel might be desirable over a larger panel. To dive deep into this discussion and really analyze all the angles of these questions, the guys have invited a special guest to join them – Amrutesh Puranik, PhD. Amrutesh is the founder of Bespoke MultiOmics Consultants, and his perspective is very insightful as they answer the questions of “What are the benefits of having a focused biological question and data interpretation plan?”, “Why are small panels recommended for cell sorting?”, “...2024-10-141h 04The Conversational Flow - An Official Podcast of KCASHigh Content Analysis: Finding Needles in a Growing HaystackIn the 12th episode of “The Conversational Flow” podcast, Brian and Adam welcome two special guests – Sean Burke of Denovo Software and Ryan Brinkman of Dotmatics – who join them to discuss the answers to a number of specific questions about High Content Analysis: How can we manage and derive insight from higher complexity panels? How can this be standardized? Once standardized, how can the process be automated? Why is more data challenging to deal with? How can we generate meaningful answers from known and unknown antibody combinations? How can this enormous amount of data be standardized? How can this type of...2024-09-141h 03The Conversational Flow - An Official Podcast of KCASGoing Global with Spectral Flow Cytometry - US, EU and Now AustraliaDuring this special episode of "The Conversational Flow" our hosts, Brian and Adam, welcome two guests: John Bucksath, CEO of KCAS Bio, and Stefan Cross, CEO of Crux Laboratories. These two guests discuss the elements and advantages of their organizations' new partnership between KCAS' US and European locations and Crux's location in Melbourne, Australia. Recent months have seen the three locations (Crux in Melbourne, and KCAS's Philadelphia and Lyon facilities) collaborate with Cytek to globalize the use of KCAS' multiple Cytek Aurora platforms and offer advanced spectral flow cytometry universally to their customers around the world. Learn about all...2024-08-141h 06
Smart Biotech Scientist | Master Bioprocess CMC Development, Biologics Manufacturing & Scale-up, Cell Culture Innovation76: Transforming Single-Cell Analysis With Impedance Flow Cytometry w/ Furkan Gökçe - Part 2Curious about the latest innovations in cell analysis technology? Join us in this episode with Furkan Gökçe, New Business Development Manager at Amphasys, as we delve into the fascinating world of Impedance Flow Cytometry (IFC).Furkan shares how IFC is revolutionizing single-cell analysis by providing faster, more precise, and high-resolution data.Key Takeaways:Real-Time Precision: Discover how IFC allows for real-time, label-free assessments of cells, providing detailed insights into their natural state.Enhanced Process Control: Learn how IFC can detect early signs of nutrient deficiencies and contaminants, leading to timely interventions and im...2024-08-0123 minSmart Biotech Scientist | Master Bioprocess CMC Development, Biologics Manufacturing & Scale-up, Cell Culture Innovation75: Transforming Single-Cell Analysis With Impedance Flow Cytometry w/ Furkan Gökçe - Part 1Ever wondered how single-cell analysis could revolutionize bioprocessing? Join us in this episode as we delve into the cutting-edge world of impedance flow cytometry.Furkan Gökçe, New Business Development Manager at Amphasys, shares his insights on the transformative power of this technology in the biotech industry. With a background in engineering and physics, Furkan provides a unique perspective on tackling the complexities of biological systems.Key Takeaways:Enhanced Process Control: Learn how impedance flow cytometry offers high-resolution, label-free analysis of individual cells, ensuring optimal growth conditions and quality production.Understanding Cell Heterogeneity: Di...2024-07-3017 min