Shows
Royal Academy of Intl. Flow Cytometry AffairsOzette | Tools of the TradeSend a textWe're meeting with the good people of Ozette - CEO and co-founder Greg Finak, and Chief Product Officer Corrie Ortega. We discussed how Ozette can support the research efforts of the various core facilities across the land through its data exploration tool Ozette Discovery, and improve the quality of flow data with better mixing using Ozette Resolve.
2026-02-2052 minRoyal Academy of Intl. Flow Cytometry AffairsMeeting Oliver Burton | The ChUG PodcastSend a textIt was my great pleasure to meet Dr. Oliver Burton and discuss his very impactful blog Colibri-Cytometry.com. We covered his career path, and explored many of the more popular topics covered in the blog.
2026-02-201h 06
Paper Talk380-HR-IMC: Submicrometer Resolution Imaging Mass CytometryThis research introduces High-Resolution Imaging Mass Cytometry (HR-IMC), a novel method that significantly enhances the spatial detail of traditional multiplexed tissue imaging. By employing an oversampling technique and computational deconvolution, the researchers improved resolution from 1 µm to below 350 nm, matching the performance of light microscopy. This advancement allows scientists to visualize subcellular structures like mitochondrial networks and nuclear foci that were previously invisible using standard mass cytometry. Validation across diverse tissue types, including ovarian cancer cells, demonstrates that HR-IMC provides superior cell segmentation and more accurate biomarker mapping. Ultimately, the technology offers a powerful tool for studying complex biological f...
2026-01-0719 min
Future Science GroupTrends, tools and transformation: the evolution of flow cytometry in bioanalysisTrends, tools and transformation: the evolution of flow cytometry in bioanalysis by Taylor & Francis Group
2026-01-0523 min
ReSayHigh-resolution imaging mass cytometry to map subcellular structuresThis article "High-resolution imaging mass cytometry to map subcellular structures" is published in Nature.Author: Alina BollhagenJournal: NatureYear: 2025Podcast type: ShortAuthors are:Alina BollhagenViola Heinzelmann-SchwarzFrancis JacobJames WhipmanRicardo CoelhoBernd BodenmillerAffiliations:Alina Bollhagen, Bernd Bodenmiller ,Department of Quantitative Biomedicine, University of Zurich, Zurich, SwitzerlandJames Whipman1,Alina Bollhagen, Bernd Bodenmiller , Institute of Molecular Health Sciences, ETH Zurich, Zurich, SwitzerlandAlina Bollhagen, Life Science Zurich Graduate...
2025-12-1405 minRoyal Academy of Intl. Flow Cytometry AffairsIn the Service of Cytometry - Fix your own stuff! | The ChUG PodcastSend a textThe issue I have whenever we discuss negotiating service contracts is that we just skip over the most useful leverage we have: fixing stuff on our own. But then again, there is very little training and information offered on how to do just that. It was a great pleasure to have Lisa Nicholls, James Marvin and Camille Stegen to discuss what the DIY life looks like, what's in their toolbox, and what can be done going forward to get that knowledge to spread.The ChUG Podcast is not sponsored by any organizations...
2025-12-031h 03Royal Academy of Intl. Flow Cytometry AffairsThe Good, the Bad, and the Post Unmixing CompensationSend a textHere it is! The debate that will settle this business of post acquisition adjustment of your unmixing matrix. Is it an appropriate thing to do? Or will applying manual compensation to your data send you to the 9th circle of hell (the one with the puppy stuck in ice)? So fine, my awesome guests Florian Mair and Rui Gardner do not come up with the final answer during this very insightful discussion. But I think the discussion will get us flow cytometry nerds look at the issue in a different way, and offers valuable...
2025-08-241h 06Royal Academy of Intl. Flow Cytometry AffairsDavid has Money Issues (ft. Melissa Leone)Send a textWe live in strange days... The research field is facing a very challenging situation (born out of some very stupid decision making). In this ChUG episode, Melissa Leone - sales expert extraordinaire, VP of all things complicated, and now the very best core director in the land, helps David come up with a plan to reduce expenses by 10%, or increase revenues by 10%. Then we end up creating a Chicago-based marketing effort.
2025-08-241h 07
Flow StarsVirginia Litwin (International Society For Advancement of Cytometry)In this episode, Peter O'Toole is joined by Virginia Litwin, the President-Elect of the International Society For Advancement of Cytometry (ISAC).They discuss Virginia's journey from academia to industry and her pivotal role in the creation and development of CYTO Women, a committee that's having a genuine positive impact on the opportunities available for women in cytometry.She also shares her experiences in translational science, working closely with clinical trials, and her passion for standardization in flow cytometry. Watch or listen to all episodes of Flow Stars: flowstars.bitesizebio.com
2025-05-3055 min
Flow StarsSpectral Flow Cytometry SpecialLet's shift our focus from just the people behind the science to the technologies they're using.In this special episode, we dive deep into spectral flow cytometry with a panel of leading experts, featuring:• Tamar Tak, Coordinator Flow Cytometry Facility, Leiden University Medical Center• Domenico Lo Tartaro, University of Modena and Reggio Emilia• Andrew Patentreger, Senior Team Lead, Broad Institute of MIT and Harvard• Kelly Lundsten, Biotechnology Market and Technical Bioassay Consultant, Luminous Bioanalytical Consulting• John Bianchi Colangeli, Resource Technologist I, Broad Institute of MIT and HarvardThey introduce the fundam...
2025-03-211h 06Royal Academy of Intl. Flow Cytometry AffairsChatting Around A Whole New Dumpster Fire | The ChUG PodcastSend a textAs much as I want to keep the ChUG podcast light and useful, we've entered a time where every piece of news we hear from the current administration is highly concerning. The media is discussing the stock market using stock images of people grabbing their face with their hands, we hear of NIH cuts and layoffs, and uncertainty is the only variable that is constant. I was happy to have Camille Stegen, Kathy Kluepfel, and Daniel Vocelle to discuss and provide some sense of clarity on how to move forward. So this is not...
2025-03-191h 24Royal Academy of Intl. Flow Cytometry AffairsBuy or Lease with First American (with Andrew Card and Nick Borkowski) | Tools of the TradeSend a textTo Buy or to Lease? Finding funds for the purchase of flow cytometry equipment in your core facility can be challenging. In this first installment of the Tools of the Trade podcast, we discuss the leasing option offered by First American with Andrew Card and Nick Borkowski. RAIFCA's Tools of the Trade podcast highlights lesser-known tools available to flow cytometry core facility staff and users. We do not necessarily endorse the products discussed; our goal is to help listeners gain a better understanding of them.
2025-03-1955 min
Bloody MindedEpisode 28 - In the Lab: Flow Cytometry, Mass Spectrometry & Cytogenetics in MMCan’t get enough of obscure haematological lab chat, can you? Well we’ve got good news for you! Join us today as we delve even deeper into the arcane depths of flow cytometry, mass spectrometry and cytogenetics as they apply to myeloma. We promise we didn’t just make these words up.02:23 - Flow cytometry: overview04:01 - The flow cytometer: practical aspects09:01 - How we interpret flow11:44 - Myeloma flow markers14:32 - Quality in flow21:26 - Mass spectrometry25:59 - Cytogenetics27:46 - Cytogenetics assay principles30:30 - Karyotype abnormalities31:44 - FISH...
2024-12-1742 min
That's What I Call Science!Episode 263: The Australasian Cytometry SocietyJoin us for this week’s episode, in which Olly and Georgia make their way through the poster session at the Australasian Cytometry Society’s annual 2024 conference! Cytometry may not be a word you’ve heard before, but is all about cells. And, by studying the cells in our bodies, the researchers in today’s episode have been making leaps and bounds in medical research in order to help the patients that need them.Show theme music: Kevin MacLeodHost: Olly Dove (@olly_dove)Co-Host: Georgia Stewart (@ggstew25)Prod...
2024-11-2427 minRoyal Academy of Intl. Flow Cytometry AffairsChUG podcast #21 - Meet Locally, Flow GloballySend a textRegional flow cytometry are where it's at. This is what we have decided here at ChUG. We invited Aja Rieger to discuss the CoLD Summit, Karla Gorena to talk about FlowTex, and Daniel Vocelle to cover GLIIFCA. If you have unquestionably attended CYTO without any second look at other meetings, you've been missing out and this podcast is for you!Please note: David got pretty excited and dropped some expletives in the first minute of the podcast. Then he got a hold on himself. Edit 11/13/24: Karla Moncada Gorena is now Director o...
2024-11-131h 17
Inside BiotechCareer Pivot to Biotech Product Management with Sara Tafoya from Sony BiotechnologyIn this episode of Inside Biotech, we explore the transition from academia to product management with Dr. Sara Tafoya, Global Product Manager at Sony Biotechnology. Sara’s passion lies in helping STEM graduate students and early career professionals recognize the value of their technical expertise beyond the bench. She shares her journey from PhD to product management, discussing how she bridges technical know-how with customer advocacy and sales to drive innovation in biotech. If you've ever wondered how to apply your niche academic skills to roles outside of research, this episode is packed with actionable insights. Listen in as Sa...
2024-09-1857 min
Smart Biotech Scientist | The CMC and Bioprocessing Podcast for Process Development and Manufacturing Leaders76: Transforming Single-Cell Analysis With Impedance Flow Cytometry w/ Furkan Gökçe - Part 2Curious about the latest innovations in cell analysis technology? Join us in this episode with Furkan Gökçe, New Business Development Manager at Amphasys, as we delve into the fascinating world of Impedance Flow Cytometry (IFC).Furkan shares how IFC is revolutionizing single-cell analysis by providing faster, more precise, and high-resolution data.Key Takeaways:Real-Time Precision: Discover how IFC allows for real-time, label-free assessments of cells, providing detailed insights into their natural state.Enhanced Process Control: Learn how IFC can detect early signs of nutrient deficiencies and contaminants, leading to timely interventions and im...
2024-08-0125 minSmart Biotech Scientist | The CMC and Bioprocessing Podcast for Process Development and Manufacturing Leaders75: Transforming Single-Cell Analysis With Impedance Flow Cytometry w/ Furkan Gökçe - Part 1Ever wondered how single-cell analysis could revolutionize bioprocessing? Join us in this episode as we delve into the cutting-edge world of impedance flow cytometry.Furkan Gökçe, New Business Development Manager at Amphasys, shares his insights on the transformative power of this technology in the biotech industry. With a background in engineering and physics, Furkan provides a unique perspective on tackling the complexities of biological systems.Key Takeaways:Enhanced Process Control: Learn how impedance flow cytometry offers high-resolution, label-free analysis of individual cells, ensuring optimal growth conditions and quality production.Understanding Cell Heterogeneity: Di...
2024-07-3017 min
Flow StarsFlow Stars Recorded Live at CYTO 2024In this special episode of Flow Stars recorded live at CYTO 2024, our panel of esteemed guests, representing a cross-section of cytometry users, discuss the biggest matters in flow cytometry. They explore the capabilities of current cytometers and what future developments should focus on. They also consider the importance of making cytometry equipment more affordable and the level of technical support provided by manufacturers. Plus, the panel also discusses the importance of encouraging young researchers to use flow cytometry for small particle analysis and whether it's normal to name your instruments!With:• Cl...
2024-07-1034 min
BENCH to BEDSIDEExplore the Cores: Illuminating Discoveries With CHOP’s Flow Cytometry CoreIn the second part of our Explore the Cores series, Florin Tuluc, MD, PhD, Director of the Flow Cytometry Core Laboratory at CHOP Research Institute, sits down with host Susan Furth, MD, PhD, Chief Scientific Officer, to talk about what makes the Flow Lab a unique and valuable resource for the CHOP scientific community. Spoiler: It’s one-part state-of-the-art technology and two parts a team of incredible individuals with a passion for pediatric health.
2024-06-2815 min
Mentors at Your Benchside4 Fixatives for Histology and Cytometry: Perfect Your Preservation#108 — What should you use to fix your cells? Alcohols or aldehydes? Gluteraldehyde or formaldehyde? And how long will your cells stay fixed?This episode explains the four main fixatives for histology and cytometry and when to use them. It also provides some practical tips to ensure your fixation works and explains the benefits of combining fixatives. Check out the corresponding article for links to related resources. [1] To learn more about autofluorescence and the controls you need to check for it, read this article. [2]Resources1. 4 Fixatives for Histology and Cytometry. Perfect Your Preservation. Av...
2024-06-2506 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #20 - All the Flow Podcasts Are HereSend a textIt was a pleasure to host Dr. Peter O'Toole, Director of the BioScience Technology Facility and Head of Imaging and Cytometry core at the University of York. He is also the host of the other, way more popular, flow cytometry dedicated podcast Flow Stars. We discussed all things podcasting, creating workshops and setting up meetings, and many more topics. We also discussed the RMS - visit www.rms.org.uk for more details and how to become a memberSubscribe to @BitesizeBio - home of Flow Stars and The Microscopists
2024-06-241h 20Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #19 - In the Service of Cytometry: The ABRF 2024 SessionSend a textThe Association of Biomolecular Resource Facilities (ABRF) had its 2024 annual meeting in Minneapolis and I was asked to chair the Service Contract Session. Pro tip: if that happens to you, you need to recruit Julie Auger, Executive Director at the Salk Institute, and she'll find equally great panelists for the session. It was a pleasure to have Brooke Bean Massani, Director of Research Support Services at the University of Arizona, and Roy Martin, Senior Field Marketing Manager for Biological Mass Spectrometry at Waters join the discussion. And for good measure, we had super secret...
2024-06-141h 16
Citeline PodcastsPodcast: Facilitating Patient Monitoring in CAR-T DevelopmentLearn how molecular technology is enhancing patient safety for CAR-T clinical trials.
US FDA recently initiated class safety labeling changes after evaluating the safety of CAR-T cancer treatment therapies. What does this mean for developers in the CAR-T landscape? Listen to your free podcast to find out.
Listen in on the conversation as experts from Cerba discuss facilitating patient safety measures in CAR-T therapies by accurately identifying the integration of viral vectors, utilizing flow cytometry, and more.
Listen now as experts discuss answers to:
· What patient monitoring is required by the FDA?
· What molecular technology does Cerba use and how do...
2024-04-2919 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #18 - Cezary and Daniel explain Spatial Biology to DavidSend a textSpatial Biology is an emerging technology that can get a heck of lot confusing for David. When David doesn't understand something, he asks people to help. Be like David. Ask Cezary and Daniel to explains things to you.
2024-04-121h 19Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #17 - Chatting Around the Dumpster Fire III - Allegedly and PresumablySend a textThe gang is back to discuss all kinds of things happening is Flowland! Ryan Duggan, Bert Ladd, Rebecca Bultema, and that other guy discuss which meetings look good this year, the range of ideas floating around reagents used to clean and maintain instruments, the problem of single stain controls when unmixing data, and careers outside of a flow core.
2024-03-281h 25
Marie Curie Science Research CenterFlow Cytometry By Dr. Marjan Assefi at the Forbes ScienceFlow Cytometry By Dr. Marjan Assefi at the Forbes Science
2024-03-2802 min
Mentors at Your Benchside7 Top Tips to Make the Most of Your Flow Cytometry Training#99 — So you’ve got your flow cytometry training booked and are one step closer to that precious data.But if you want to hit the ground running and get some useful data from your samples, there are some little things you'll need to do.These include reading up on a bit of background theory, understanding the capabilities of different types of cytometers, and thinking about what you want to learn from your experiment.In this episode of Mentors At Your Benchside, we've compiled cytometry training advice from a core facility manager to help you...
2024-02-2711 min
Listen In - Bitesize Bio Webinar AudiosBenefits of Imaging Data in Flow Cytometry Experiments: A Panel DiscussionExperience the transformative power of unbiased, data-driven cell analysis with access to over 25 image-derived label-free parameters, empowering you to easily assess sample quality, optimize gating strategies, and validate rare events.In this episode, learn how automated image analysis combined with flow cytometry can help improve data accuracy and precision, optimize and verify gating strategies, and discover novel biology, such as cell–cell interactions.Join a panel of experts as they discuss the features, benefits, and applications of a paradigm-shifting technology in flow cytometry with the Invitrogen™ Attune™ CytPix™ Flow Cytometer.Watch the full presenta...
2024-02-1241 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #16 - In the Service of Cytometry with Ray LanniganSend a textWe continuing our exploration of service contracts in the Flow Cytometry with none other than Ray Lannigan, formerly Vice President of Global Sales and Services at Cytek. We couldn't ask for a better guide to explain the details of service contracts form the industry point of view. Here we discuss how service contracts are priced, what kind of leverage is available to negotiate with manufacturers, the other options for those who may want to look elsewhere for service, tracking service on your instruments, rights to repairs, and many other topics.
2023-12-121h 34Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #15: Spectral Flow - What You Don't Know Is Out To Kill YouSend a text'What you don't know is out to kill you' had been the subtitle for the CAT Facility flow basics class for over a decade. It reminds us that the life of a flow cytometrist is filled with peril and danger. One day you're on the top of the world, thinking you know everything about spectral flow. Then some company comes out with a bunch of marketing material and data that make you question all that you ever hold dear. So we invited Ryan Duggan, Dave Adams and Rachael Sheridan to discuss what's going on...
2023-12-1259 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #14: Cytometry on Air #3: Cell SortingSend a textRyan ran a discussion on the cell sorters available on the market on the Cytometry on Air podcast, roughly 10 years ago. It's now 2023 and we invited Dave Adams back along with Bill Eades to discuss how things look nowadays. We talked about the premature deployment of cell sorters, ultra-specialization of platforms, and planned obsolescence. We covered the most popular droplet-based platforms available, as well as the microfluidics systems.
2023-10-141h 23Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #13 - In the Service of Cytometry (with Tony Leger)Send a textWe're thrilled to host Tony Leger, Director of Automation Laboratory Technology (ALT), for a discussion on instrument service in the field of Flow Cytometry. If you have frustrations regarding the cost of service contracts, or the difficulty around repairing your own instruments, this discussion should provide some great insights. We're hoping to expand the 'In the Service of Cytoemtry ' series to multiple actors in the field to better understand what is going on, but we wanted to start discussion to begin with the voice of Tony Leger. His care for right to repair...
2023-09-192h 11
Flow StarsLive at CYTO 2023 with Jessica Houston, Pratip Chattopadhyay, and Paul Wallace#S4:E1 — In this special episode of Flow Stars recorded live at CYTO 2023, host Peter O'Toole welcomes a panel of distinguished experts, including Jessica Houston (New Mexico State University), Pratip Chattopadhyay (NYU Langone Health (PIL)), and Paul Wallace (Roswell Park Cancer Comprehensive Center).Join them for an enlightening conversation as they dive into the future of flow cytometry and provide their unique perspectives on how the utilization of flow cytometry could evolve in the next 5-10 years.But that's not all! Our guests share their favorite lab tools, revealing a world beyond cytometers. They reveal wh...
2023-09-0636 min
Venture In The SouthE82: Nirvana Sciences Innovates on Flow CytometryNirvana Sciences has innovated traditional flow cytometry, a longstanding cellular diagnostic tool, in a unique and powerful way to dramatically increase the efficiency and utility of this tool for a wide range of diagnostic problems. Through development of proprietary bright narrowband fluorescent markers (or dyes), NS has enabled a magnitude increase in the number, fidelity and efficiency of cell markers that can be measured from a single sample. This has positioned NS with strong IP for an acquisition by a major diagnostics company.Follow David on X at https://x.com/DGRollingSouth...
2023-09-0427 min
Listen In - Bitesize Bio Webinar AudiosGarbage In, Garbage Out: Sample Prep for Flow CytometryWhat's more disappointing than running your flow cytometry sample to discover it's rubbish? Especially considering time on cytometers can be precious, even more so if you've booked onto an in-demand one at a busy core facility. You've done all the work and all the waiting—for nothing. If this scenario sounds familiar, this episode of Listen In is for you.Jessica Rowley, Flow Cytometry Facility Manager at Imperial College London, explains how to prepare flow samples optimized for your experiments, to save you time and avoid disappointment.She has worked with hu...
2023-08-281h 00
CAPcastsCAP Author Chat - The Color Atlas of Flow Cytometry Just released, the new Color Atlas of Flow Cytometry offers a full range of hematolymphoid disease cases, highlighting disease categories and disease entities that may be difficult to diagnose in clinical practice. The new book can benefit students, medical technologists, and practicing pathologists, explains lead author, David Dorfman, MD, PhD, FCAP in this CAPcast interview.
2023-04-2107 min
CAPcast from the College of American PathologistsCAP Author Chat - The Color Atlas of Flow CytometryJust released, the new Color Atlas of Flow Cytometry offers a full range of hematolymphoid disease cases, highlighting disease categories and disease entities that may be difficult to diagnose in clinical practice. The new book can benefit students, medical technologists, and practicing pathologists, explains lead author, David Dorfman, MD, PhD, FCAP in this CAPcast interview.
To purchase this book, please visit CAP's estore: https://estore.cap.org/OA_HTML/xxCAPibeCCtpItmDspRte.jsp?section=10044&item=747354&sitex=10020:22372:US
2023-04-2107 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #12 - Imaging Flow Cytometry. What's going on?Send a textWe're back! We wanted to make sense of the numerous imaging flow cytometers that have hit the market over the last few years, notably the Attune CytPix and the BD FACS Discover S8. Ryan Duggan, Director Emeritus Pat Simms, Daniel Vocelle and that other guy discuss the potential use of the technology, and harp on an imaginary Image Stream Cell sorter.
2023-03-131h 15
Laboratory Considerations for Clinical TrialsFlow Cytometry as an IVD technology for Companion DiagnosticsIn this episode, Megan McCausland, Scientific Advisor for Flow Cytometry at Q2 Solutions, and Scott Bornheimer, Associate Director of Medical and Scientific Affairs at BD Biosciences talk about the enormous potential of flow cytometry as a clinical diagnostic tool.
Currently, pharma companies are looking for simplified, standardized flow cytometry tests to help with enrolling patients and understanding outcomes in their studies.
In the longer term, Megan McCausland notes what will lead to the use of flow as a CDx:
“I think the drug development landscape really continues to evolve towards more personalized medicine. As t...
2022-11-3020 min
Smart TalkDr Anna Brooks on the looming crisis of Long CovidThe term 'Long Covid' was coined in May 2020 but the virus became a pandemic before the most common post-viral condition was taken seriously. Dr Anna Brooks explores what we now know about it.The term 'Long Covid' was coined in May 2020 but the virus became a pandemic before the most common post-viral condition - (ME/CFS) - was taken seriously.So what do we know now about it? Dr Anna Brooks explores Long Covid in a talk for Auckland University's Raising the Bar 2022.The global Covid-19 pandemic has changed life as we once...
2022-09-2749 min
Dermasphere - The Dermatology Podcast87. Flow cytometry with Dr. Vermeer - Ruxolitinib cream for vitiligo - Baricitinib for AA - Roflumilast for psoriasis - Tapinarof for psoriasisFlow cytometry with Dr. Vermeer -
Ruxolitinib cream for vitiligo -
Baricitinib for AA -
Roflumilast for psoriasis -
Tapinarof for psoriasis -
Connect with Dr. Vermeer!
https://www.universiteitleiden.nl/en/staffmembers/maarten-vermeer#tab-1
Luke's Practical Dermatology for Primary Care course: https://registration.socio.events/e/pdpc2022
Michelle and Luke will both be speaking at the upcoming Texas Derm Society meeting! https://www.texasdermatology.org/amsimis/TDS/Meetings/Annual_Meetings/Annua_Meetings.aspx
Connect with us!
Web: https://dermaspherepodcast...
2022-09-051h 01
Listen In - Bitesize Bio Webinar AudiosIncorporating New Dyes to Simplify Panel Design/ReagentIncorporating New Dyes to Simplify Panel Design: Accelerating Reagent AvailabilityIn this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components.We will cover:How quality custom reagents in small sizes will make your panel design easierHow you can receive these reagents fast and without traditional wait times for manufacturingHow you can be confident that quality control is maintained for these custom reagentsIncorporating New Dyes to Simplify Panel DesignDr. Jorg Ruhrer, Director of...
2022-08-3141 min
Preprints in MotionMetabolic heterogeneity in macrophagesThis week we discuss macrophage heterogeneity with Graham Heieis (@GrahamHeieis), a Post-doc at the Leiden University Medical Center (@LUMC_Leiden) in the Netherlands. We speak to him about mass cytometry and spectral flow cytometry and their use to investigate heterogeneity of tissue specific macrophages with regards to metabolism. We then have our usual chat about preprints, moving labs from Canada to Scotland as well as a bit of a a whiskey discussion.
Read the full preprint: https://www.biorxiv.org/content/10.1101/2022.05.26.493548v1.full
This episode was produced by Emma Wilson and edited by John D H...
2022-08-0343 min
Mentors at Your BenchsideHow a Jellyfish Changed Biology: the Discovery and Development of GFP#7 — In this episode, we cover how jellyfish were able to have a huge impact on biology through the discovery and development of GFP.Using GFP is now ubiquitous in pretty much all fields in biology, and we'll take you through how three Nobel Laureates developed this valuable research tool. [1]Many of the applications for GFP are microscopy-based, and we'll discuss how you can utilize GFP for translational and transcriptional fusions, FRAP, FLIP and FRET experiments in the lab. [2,3]Read the full article for more useful links, hints, and tips on using GFP in yo...
2022-07-1205 min
Flow StarsGrace Chojnowski (Queensland Institute of Medical Research)#S3:E2 — Grace Chojnowski is Flow Cytometry and Imaging Facility Manager at the Queensland Institute of Medical Research.In this episode of Flow Stars, Grace joins Peter O'Toole to discuss why understanding the theory behind your experiment is key in flow cytometry, why she'd like a flow lab in the Alps, and her early career as an Australian living in London. We also hear about a funny interview story and talk candidly about how she avoided the stress of isolation and burnout at the start of her career.Watch or Listen to all episodes of Fl...
2022-06-151h 05Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #11 - 2022 in ReviewSend a textHere at ChUG in Cytometry, we aim to be the most relevant that we can be! This is why we decided to comment on events that have not happened yet! Laura Johnston, Pat Simms, Robot Ryan Duggan and David Leclerc discuss upcoming flow meetings, what's going on at ISAC, cells sorters, David is shilling for industry, and hiring new staff in SRLs. We also say goodbye to Laura who is moving to back to Seattle.
2022-04-191h 21
Future Science GroupFlow cytometry for CGT assay development: an interview with Richard CuthbertFlow cytometry for CGT assay development: an interview with Richard Cuthbert by Taylor & Francis Group
2022-04-0511 min
Flow StarsPaul K. Wallace (Roswell Park Comprehensive Cancer Centre)#S2:E6 — Paul K. Wallace of the Roswell Park Comprehensive Cancer Center was one of the first clinical cytometrists. He has had a huge influence on the field of flow cytometry, particularly in developing assays to track immune function. In this engaging episode of Flow Stars, Paul chats to Peter O’Toole about his early ambitions to become a vet, the importance of education and training in flow cytometry, and how his role has led him all over the world. We also discover what’s Paul’s favorite foods are (tip: don’t serve him liver!), whom from history he’d most...
2022-03-231h 04
New Matter: Inside the Minds of SLAS ScientistsSolving Problems for the World's GoodIn this first episode with our new host, SLAS Scientific Manager, Hannah Rosen, we talk to Steven Graves, CEO and Founder at BennuBio, Inc about their new, high-throughput, multiple parallel stream system for flow cytometry. Steve shares their biggest challenges in developing this technology and their goal in getting it out into commercial world where it can do the most good. Listen in to find out how their new system, the Velocyt®, can change research in flow cytometry and what makes it a game changer. BennuBio was awarded the Ignite Award at the SLAS2022 International Conference and Exhib...
2022-03-1614 min
World Happiness PodcastProfessor Andrew Harman on a Calm Mind, Buddhism and ScienceOur guest today is Dr. Andrew harman, a Professor of Virology and Immunology at the University of Sydney School of Medicine and an ordained Buddhist who has much insight on how Buddhist practices and philosophy can help us deal with change, restlessness and uncertainty in our fast moving world. He shares how acceptance of your reality can help bring about calm. Dr. Harman and Pooja talk extensively about how we may need to reset our definition of "happiness" – instead of desperately seeking out happiness through exciting, impermanent experiences, things, people or moments, we might instead think of happ...
2022-03-1244 min
The Calm & Free Podcast with Pooja Mottl#92 - Professor Andrew Harman on a Calm Mind, Buddhism and ScienceOur guest today is Dr. Andrew harman, a Professor of Virology and Immunology at the University of Sydney School of Medicine and an ordained Buddhist who has much insight on how Buddhist practices and philosophy can help us deal with change, restlessness and uncertainty in our fast moving world. He shares how acceptance of your reality can help bring about calm. Dr. Harman and Pooja talk extensively about how we may need to reset our definition of "happiness" – instead of desperately seeking out happiness through exciting, impermanent experiences, things, people or moments, we might instead think of happ...
2022-03-1044 min
Flow StarsAlice Givan#S2:E5 — Alice Givan, now retired, was previously the director of the Flow Cytometry/Fluorescence Imaging Core at the Norris Cotton Cancer Center/Dartmouth Medical School in New Hampshire and author of the classic text Flow Cytometry: First Principles.In today's episode of Flow Stars, Alice joins Peter O'Toole to discuss how funding issues led to her first job working with a flow cytometer, her love of teaching and why every flow lab needs a microscope. Alice also discusses how she met her husband of more than 50 years in graduate school and why she spends to...
2022-03-091h 02
Flow StarsKylie Price (Malaghan Institute of Medical Research)#S2:E3 — Kylie Price is the Head of Research Technology at the Malaghan Institute of Medical Research in New Zealand and current Secretary of ISAC. She is a tireless proponent of the benefits of cytometry and of improving science and technology in New Zealand. She’s also pretty tireless in general, it turns out, as we discover in this episode of Flow Stars.Whether it’s introducing her children to the wonders of the natural world, climbing, diving, horse-riding, or beekeeping, Kylie’s passion is evident in abundance. We learn more about how she got into cytometry, and her...
2022-02-091h 06
Unravelling Science PodcastEpisode 47: Dr Barry Moran: Flow Cytometry, Part Time PhD & Work/Life BalanceDr Barry Moran core facility manager of the Flow Cytometry Facility, at the Trinity Biomedical Sciences Institute is my guest this week. He chats to me about his 'unconventional' journey through academia, deciding to do a part time PhD while running the Cytometry core facility and why the 'journey-man' in him may be relatable.
Barry also discusses how flow cytometry can advance our scientific knowledge and its benefits particularly in the context of immunology. He describes his own research examining immune dysfunction in the skin disease hidradenitis suppurativa and why this is research that adds value to p...
2022-02-0853 min
Flow StarsPratip Chattopadhyay (Founder of Talon Biomarkers)#S2:E2 — In the latest episode of Flow Stars, Peter O’Toole is joined by the inspiring Pratip Chattopadhyay, who has had an illustrious career in the fields of flow cytometry and immunoassays and was the founding Director of NYU Langone Health’s Precision Immunology Laboratory (PIL). He is also the founder of startup biomarker discovery engine Talon Biomarkers. Today we discover why Pratip believes in bringing science into the community, why he can’t resist a courtroom drama, and how flow cytometry aligns with his love for telling stories. We’ll hear about early ambitions to be an astr...
2022-01-261h 06Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #10 - The Flood of '21Send a textThe CAT Facility lost a few instruments due to some water damage a few months ago and we turned to an Insurance Group to help us out. It's not a common situation for anyone (hopefully), so here's some thoughts on our experience and what can be done to avoid it as much as possible.
2021-11-1722 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #9 - Chatting Around the Dumpster FireSend a textThe gang is back! Pat Simms, Laura Johnston, Ryan Duggan, and that other guy discuss the latest news in the flow cytometry world in this second edition of Chatting Around the Dumpster Fire. They talk about hiring staff, getting back to work in the COVID era, creating online content in flow, the great floods in history, the Quanteon, CYTO 2021 (it seems they didn't see much of it), and the future of cytometry, both spectral and mass.
2021-08-241h 35Royal Academy of Intl. Flow Cytometry AffairsChUG #8 - Dealing with autofluorescence in spectral flow cytometrySend a textWe host Monica Delay, US Manager for Technical Application Support at Cytek Biosciences, for a discussion on autofluorescence (AF) and the tools to deal with it in Spectral Flow Cytometry. We use figures to showcase some problems with AF which will be missing from this audio version of our talk. You can visit our website at Chugcytometry.com to find the video, along with a list of links to webinars and publications dealing with AF.
2021-07-191h 22
Flow StarsPaul Robinson (Purdue University)#S1:E6 — Many of us owe a debt of thanks to Paul Robinson of Purdue University for developing and maintaining the flow cytometry listserv, and for writing the Handbook of Flow Cytometry Methods. In this episode of Flow Stars, we’ll learn more about how these projects came about. We’ll also discover more about Paul’s passions, his scientific inspirations, and what compelled him to climb Mount Everest. In this thought-provoking chat, we’ll hear about some of the challenges that Paul has faced in his career, how he has overcome them, and why he keeps an ice axe in his...
2021-07-1459 min
Flow StarsPaul Smith (Cardiff University)#S1:E5 — In this episode of Flow Stars, we’re joined by Paul Smith of Cardiff University, who has spent his impressive career using flow cytometry and imaging to tackle problems in cancer biology. We’ll hear more about Paul’s views on the importance of collaborative working, why he hates following recipes, and why you should think carefully about the toys you buy your children. Paul will also let us in on some secrets to successful grant applications, his scientific inspirations, and where he sees the field of cytometry going in this engaging and entertaining interview.Watch or...
2021-06-301h 04
MDforLivesClinical flow cytometry in cancer diagnosis and immune system disordersThis episode is also available as a blog post: https://mdforlives.blog/2021/06/21/flow-cytometry-in-cancer-diagnosis-and-immune-system-disorders/
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2021-06-2105 minRoyal Academy of Intl. Flow Cytometry AffairsChUG #7 - There is no I in 'cell sorter development'Send a textOur guest is our very good friend James McCracken. We explore his career path from his post-doc at The University of Chicago, to becoming the Core Facility Director of the University of Louisville Diabetes and Obesity Center, to his current position as the Commercial Marketing Manager with Beckman Coulter Life Sciences. He has been deeply involved in the development of the newly released CytoFlex SRT cell sorter, so we also discuss the ins and outs of flow cytometry instrumentation development in industry.
2021-06-141h 06Royal Academy of Intl. Flow Cytometry AffairsChUG #6 - Considerations on Online TrainingSend a textOur guests this week are Kathy Daniels, Derek Davies, and Rui Gardner. You will recognize them from the work they have done in training Flow Cytometry users over the year. They have also developed an impressive amount of online content during the pandemic, including the OpenFlow Cytometry series available on YouTube. We sat down to discuss the ins and outs of online training, the opportunities and pitfalls that we have experienced, and some of the features that could stay with us once we are back in a normal environment.
2021-05-111h 14
Flow StarsTim Bushnell (URMC) & Alfonso Blanco (UCD)#S1:E1 — Our first episode of Flow Stars features not one, but two big hitters in the world of flow cytometry, which creates a dynamic and energetic conversation. We find out about Tim’s love for brewing, including how he taught his kid to brew as a science lesson and discuss Alfonso’s passion for kicking people (in organized sports) and how his Olympic hopes were dashed by an unfortunate knee injury.While we get to know Tim and Alfonso on a personal level, work and flow cytometry is still a big focus, with both sharin...
2021-05-0555 min
iGEM Foundation ChannelWeek #6A: Quantifying fluorescence and cell phenotypes with flow cytometry 1/3More info: https://2020.igem.org/Measurement/Webinars
This webinar begins with an introduction to flow cytometry, including how these instruments operate and the types of data that they produce. The second block of the webinar focuses on calibration of measurements for fluorescence and cell size, and on debugging such measurements. Finally, we will end this session with a discussion of how to interpret and debug calibrated flow cytometry data.
Part 1/3: https://youtu.be/ODF-9L2FLbg
Part 2/3: https://youtu.be/6jCxWe9QsUM
Part 3/3: https://youtu.be/WOhPj5Csog0
2021-04-1700 miniGEM Foundation ChannelWeek #6A: Quantifying fluorescence and cell phenotypes with flow cytometry 3/3More info: https://2020.igem.org/Measurement/Webinars
This webinar begins with an introduction to flow cytometry, including how these instruments operate and the types of data that they produce. The second block of the webinar focuses on calibration of measurements for fluorescence and cell size, and on debugging such measurements. Finally, we will end this session with a discussion of how to interpret and debug calibrated flow cytometry data.
Part 1/3: https://youtu.be/ODF-9L2FLbg
Part 2/3: https://youtu.be/6jCxWe9QsUM
Part 3/3: https://youtu.be/WOhPj5Csog0
2021-04-1700 minRoyal Academy of Intl. Flow Cytometry AffairsChUG #5 - Meeting Julie AugerSend a textThis is a reunion episode of kind! Our guest is Julie Auger, Executive Director of the RCFP at UCDavis, but also the architect of the Office of Shared Research Facilities at the UoC. And the co-founder of ChUG itself!! It a pleasure to host Julie as we discuss the early days of the OSRF, and the model of centralized administration for core facilities.Visit us at chugcytometry.com for more discussions.
2021-04-141h 15Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #4 - Meeting Pratip ChattopadhyaySend a textIn this episode, we sit with Dr. Pratip Chattopadhyay, Associate Professor in the Department of Pathology and Director of the Applied Immunology Core at NYU Grossman School of Medicine, to discuss his paradigm breaking work in flow cytometry, immunology and insights into career development for us all.Visit us at ChUGCytometry.com for more discussions.
2021-03-161h 18Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #3 - The Once and Future Flow Cytometry Analysis ToolsSend a textWe’re hosting President and Founder of De Novo Software, David Novo, and Cytek Application Scientist, Geoff Kraker, to discuss analysis software tools from turn of the century to present day.
2021-02-131h 27
SciPodExploring Symbiotic Relationships Using Flow Cytometry - Dr Toshiyuki Takahashi, National Institute of Technology in Miyazaki, JapanSymbiosis is the interaction between two species that offers benefits to one or both of the organisms involved. One important type of symbiosis that has been instrumental in advancing evolution is endosymbiosis, where one of the organisms lives inside the other. Endosymbiosis has typically been studied using microscopy, but Dr Toshiyuki Takahashi from the National Institute of Technology in Miyazaki, Japan, proposes that a technique called flow cytometry can offer more detailed insights into endosymbiotic relationships and advance our understanding of these important associations.
2020-08-0708 min
The Lung Science Podcast: An AJRCMB PodcastEpisode 13: Flow CytometryDr. Andrea Yu sits down with Dr. Robert Tighe to discuss everything you wanted to know about flow cytometry (but were afraid to ask).
2020-05-0731 min
HumAIn PodcastMachine Learning with R, the tidyverse, and mlr by Hefin Rhys[Audio] Podcast: Play in new window | DownloadSubscribe: Google Podcasts | Spotify | Stitcher | TuneIn | RSSHefin Rhys is a Senior Scientist (flow cytometry) at UCB. He completed his PhD at the William Harvey Research Institute in Queen Mary University of London in 2017, and graduated from my MPharmacol degree from the University of Bath in 2013. His main academic interests are conventional, imaging and small particle flow cytometry, data science and machine learning. Episode Links: Hefin Rhys’ LinkedIn: https://www.linkedin.com/in/hefin-rhys/ Hefin Rhys’ Twitter: @HRJ21Hefin Rhys...
2020-04-0246 min
CytoFluo@ICGEB 2019A. Zucchetto - Flow cytometry for basic research and diagnosis of lymphoid malignanciesAntonella Zucchetto, CRO Aviano, Italy speaks on "Flow cytometry for basic research and diagnosis of lymphoid malignancies". This movie is part of "Flow cytometry: from basic principles to advanced application" Course, 8-10 May 2019, ICGEB Trieste, Italy.
2019-08-2632 minCytoFluo@ICGEB 2019E. Lugli - High content flow cytometry to dissect memory T cells responsesEnrico Lugli, Humanitas Milano, Italy, speaks on "High content flow cytometry to dissect memory T cells responses". This movie is part of "Flow cytometry: from basic principles to advanced application" Course, 8-10 May 2019, ICGEB Trieste, Italy.
2019-08-2649 minCytoFluo@ICGEB 2019S. Arioli - Flow cytometry applications in Food Microbiology: not only cell counting!Stefania Arioli, UniMilano, Italy, speaks on "Flow cytometry applications in Food Microbiology: not only cell counting!". This movie is part of "Flow cytometry: from basic principles to advanced application" Course, 8-10 May 2019, ICGEB Trieste, Italy.
2019-08-2653 min
The Stem Cell PodcastEp. 147: “Targeted Repair in Blood Stem and Progenitor Cells” Featuring Dr. Jennifer Adair
Guest:
Dr. Jennifer Adair is an Assistant Member in the Clinical Research Division at Fred Hutchinson Cancer Research Center. Her lab uses a combination of cell biology, molecular biology, chemistry, engineering, nanomedicine and bioinformatics to develop new gene therapy treatments for many different diseases, including cancer. Her goal is to develop safe, cost-effective and clinically relevant applications for gene therapy that can be implemented worldwide.
Featured Products and Resources:
Science in Seattle
StemSpan SFEM II
Resources and Links
Possible Drug Target for Heart Condition Identified – Researchers have found that a...
2019-07-231h 23
Just ScienceJust Imaging Flow Cytometry_2019 NIJ R&D_108In episode ten of the 2019 R&D season, Just Science interviews Dr. Christopher Ehrhardt, professor at Virginia Commonwealth University, about a method for determining tissue type, age of evidence, and contributors from biological mixtures using cellular autofluorescence signatures.
It goes without saying that cells collected from different parts of the body look different. Buccal, vaginal, epidermal, and blood cells all have unique intrinsic properties. However, when they are combined, it can be difficult to discern what components are actually in the mixture. Using Imaging Flow Cytometry, Dr. Ehrhardt has found a way to differentiate between cell types, estimate cellular age...
2019-07-2234 min
iBiology Videos Without SubtitlesSusanne Heck: Mass CytometryDr. Susanne Heck begins her talk by explaining why we might choose to use mass cytometry rather than other types of flow cytometry. Traditional flow cytometry is typically limited to the detection of about a dozen parameters in one sample due to overlap between the emission spectra of fluorochromes used to label antibodies. Mass cytometry, on the other hand, allows for the detection of up to 50 parameters in one sample because antibodies are labelled with metal isotopes and separated based on their mass. Heck goes on to explain which metal isotopes are typically used for mass cytometry and why, and sh...
2019-06-3038 min
Future Science GroupExosomes in flow cytometry: an interview with Joyce SlusserFind out more about the role of exosomes in flow cytometry studies in this interview Joyce Slusser (KCAS; KS, USA).
2019-05-0108 min
Future Science GroupFlow cytometry: an interview with Clara Brando and Ted BaginskiFind out more about current challenges and recent developments in seen in the field of flow cytometry in this interview with Clara Brando and Ted Baginski (Eurofins; MO, USA).
2019-05-0119 min
CAR-TT Cell IsolationIn patient's T Cell Isolation, flow cytometry analysis of isolated blood products ensures that a sufficient number of healthy and functional T cells being used for processing and re-infusion. https://www.creative-biolabs.com/car-t/flow-cytometry-application-in-car-t-system.htm
2019-04-2700 min
PhD Transition Report On-DemandWhat PhDs Need To Know About Leadership & How To Become A Career Multiplier
Skip ahead to:
05:40 – Show Me the Data23:35 – What PhDs Need To Know About Leadership w/ Liz Wiseman44:20 – Importance Of Continued Technical Training w/ Tim Bushnell, Ph.D.56:20 – Project Manager Career Track w/ Orly Levitan, Ph.D.
Do want to move beyond entry level positions?
Ready to learn more about how you can develop your leadership skills?
In this episode of Cheeky Scientist Radio we are very excited to be joined by Liz Wiseman, renowned Researcher, Executive Advisor, CEO and Author. As a PhD you are qualified for management-level positions...
2019-03-291h 13Laboratory Considerations for Clinical TrialsMonitoring the Tumor Micro-environment with Flow Cytometry - Mark EdingerMark Edinger is the Scientific Advisor for Flow Cytometry at Q2 Solutions. In this episode, he describes recent advances in flow cytometry and what that means for immuno-oncology trials.
New instruments, reagents and software are enabling researchers to monitor 30-some markers simultaneously to get a better picture of the tumor micro-environment and the interactions taking place.
For example, there's a whole list of new checkpoint inhibitors, like PD-1, CTLA-4, CD-1-52 and a multitude of other CD-47 that have been described, and their ligands on T cells and immune cells, not just T cells, that...
2019-03-0520 minCAR-TCAR T CharacterizationFlow cytometry is a widely used laser- or impedance-based method for CAR T Characterization by analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations and analyzing cell size and volume. https://www.creative-biolabs.com/car-t/flow-cytometry-application-in-car-t-system.htm
2019-03-0100 minCAR-TT Cell IsolationIn T Cell Isolation, flow cytometry analysis of isolated blood products is provided to ensure that a sufficient number of healthy and functional T cells being used for processing and re-infusion. https://www.creative-biolabs.com/car-t/flow-cytometry-application-in-car-t-system.htm
2019-01-3000 min
Anna & Lue?????????????Initial Diagnostic Workup of Acute Leukemia GuidelineThe purpose of this podcast is to educate and to inform. This is not a substitute for professional medical care and is not intended for use in the diagnosis or treatment of individual conditions. Guests on this podcast express their own opinions, experience, and conclusions. The mention of any product, service, organization, activity, or therapy should not be construed as an ASCO endorsement.
[MUSIC PLAYING]
Hello and welcome to the ASCO Guidelines podcast series. My name is Shannon McKernin and today I'm interviewing Dr. Valerie de Haas from Princess Máxima Center for Pediatric Oncology in the Netherlands, lead author o...
2018-12-0312 min
Future Science GroupFlow cytometry: a podcast with Christoph EberleFind out more about the flow cytometry in this Bioanalysis Zone interview with Christoph Eberle (Charles River Laboratories), as part of our 'In the Zone' feature. You can find out more about flow cytometry on the website, at: http://bit.ly/2RuwAKi
2018-10-0813 min
Brave Your FearChaquetta Grace – Alpha Woman, Scientist, Bride#10 Episode with Chaquetta Grace – Alpha Woman Scientist Bride FEB 14, 2018 Chaquetta Grace 2004 Atlanta, GA I am a servant. I am a scientist. I am a sister. BE better. Badass Quotes: "I knew that I was pretty good, but I wasn't phenomenal." (3:15) "I knew what my potential was." (10:43) "I had no hesitation. I had no hesitation." (18:59) "It was stepping out of that comfort zone because I knew I was destined for more." (39:25) "It becomes this fire within your gut, and a need." (40:14) "I...
2018-02-1454 min
St Edmund Hall Research Expo 2015: Teddy TalksColouring-in for AdultsHow flow cytometry can help investigations into immune-mediated diseases. Flow cytometry is a technique which uses flourescent compounds of different colours to label molecules on cells. Using this approach we can investigate the cell populations responsible for causing immune-mediated diseases such as Ankylosing Spondylitis and explore how new therapies can alter the behaviour of the different cell types.
2015-06-1111 min
St Edmund Hall Research Expo 2015: Teddy TalksColouring-in for AdultsHow flow cytometry can help investigations into immune-mediated diseases. Flow cytometry is a technique which uses flourescent compounds of different colours to label molecules on cells. Using this approach we can investigate the cell populations responsible for causing immune-mediated diseases such as Ankylosing Spondylitis and explore how new therapies can alter the behaviour of the different cell types.
2015-06-1111 min
Graduate School of Systemic Neurosciences - Digitale Hochschulschriften der LMUAnti-neurofascin antibodiesNeurofascin (NF) is a cell-adhesion molecule that is found at the nodes of Ranvier. The 186
kDa isoform of neurofascin (NF186) is expressed on the axon in the exposed node, and the
155 kDa isoform (NF155) is expressed on myelinating glia at the paranode. NF186 is essential
for clustering of sodium channels to the nodes while NF155 is needed for close paranodal interactions
between myelinating glia and axons. The neurofascins are found in both the peripheral
and central nervous system (PNS and CNS).
NF-specific autoantibodies were identified in serum of multiple sclerosis (MS) patients using
a proteomics approach with two-dimensional Western blotting...
2012-11-2000 min![[Audio] BME 695L: Engineering Nanomedical Systems](https://nanohub.org/site/resources/2011/08/11877/itunes_audio.png)
[Audio] BME 695L: Engineering Nanomedical SystemsBME 695L Lecture 14: Designing and Testing Integrated Nanomedical SystemsSee references below for related reading.14.1 Introduction to integrated designs14.1.1 “Total design” but there is some order in the design process14.1.2 A brief outline of the total design process14.2 Choose autonomous or non-autonomous design14.2.1 If autonomous, will there be error-checking to correct mistargeting?14.2.2 If autonomous, does the NMS perform all of the multi-step process sufficiently to accomplish the objective?14.2.3 If non-autonomous, what form of external modulation of the in-vivo nanomedical system will be used?14.2.4 If non-autonomous, are the external interactions able to adequately control the actions of the NMS?14.2.5 Evaluate reaction of NMS to external intervention14.2.6 Compare actions of NMS with and without external intervention.14.2.7 ...
2011-11-2300 min[Audio] BME 695L: Engineering Nanomedical SystemsBME 695L Lecture 14: Designing and Testing Integrated Nanomedical SystemsSee references below for related reading.14.1 Introduction to integrated designs14.1.1 “Total design” but there is some order in the design process14.1.2 A brief outline of the total design process14.2 Choose autonomous or non-autonomous design14.2.1 If autonomous, will there be error-checking to correct mistargeting?14.2.2 If autonomous, does the NMS perform all of the multi-step process sufficiently to accomplish the objective?14.2.3 If non-autonomous, what form of external modulation of the in-vivo nanomedical system will be used?14.2.4 If non-autonomous, are the external interactions able to adequately control the actions of the NMS?14.2.5 Evaluate reaction of NMS to external intervention14.2.6 Compare actions of NMS with and without external intervention.14.2.7 ...
2011-11-2300 min