Shows
Royal Academy of Intl. Flow Cytometry AffairsThe Good, the Bad, and the Post Unmixing CompensationSend us a textHere it is! The debate that will settle this business of post acquisition adjustment of your unmixing matrix. Is it an appropriate thing to do? Or will applying manual compensation to your data send you to the 9th circle of hell (the one with the puppy stuck in ice)? So fine, my awesome guests Florian Mair and Rui Gardner do not come up with the final answer during this very insightful discussion. But I think the discussion will get us flow cytometry nerds look at the issue in a different way, and offers...
2025-08-241h 06Royal Academy of Intl. Flow Cytometry AffairsDavid has Money Issues (ft. Melissa Leone)Send us a textWe live in strange days... The research field is facing a very challenging situation (born out of some very stupid decision making). In this ChUG episode, Melissa Leone - sales expert extraordinaire, VP of all things complicated, and now the very best core director in the land, helps David come up with a plan to reduce expenses by 10%, or increase revenues by 10%. Then we end up creating a Chicago-based marketing effort.
2025-08-241h 07
The Conversational Flow - An Official Podcast of KCASThe Nitty Gritty of Global Harmonization (w/ Special Guest, Karla Moncada)In this episode, the hosts introduce Karla Moncada, who shares her professional journey from Flowcore management to her current role at KCAS, and unveils her new KCAS video series, Karla’s Corner, aimed at fostering year-round conversations within the flow cytometry community. Karla emphasizes the value of personal connections in scientific collaboration and how her career has blended customer service with cutting-edge science. The main topic of the episode focuses on global harmonization in flow cytometry, particularly in phenotyping myeloid-derived suppressor cells (MDSCs) across 23 international labs. The discussion highlights challenges such as inconsistent gating strategies, variations in reagent availability, an...
2025-07-1450 minThe Conversational Flow - An Official Podcast of KCASFlow Cytometry & Autoimmunity – A High Affinity Pharmacodynamic MatchIn episode 21 of “The Conversational Flow” podcast, our hosts, Adam and Brian, explore the transformative power of gene therapy, starting things off by highlighting a compelling case involving CRISPR with milestones that once seemed impossible. They dive into the science behind CRISPR’s precise gene-editing capabilities and its potential for rapid, customizable treatments targeting rare and complex genetic mutations. The conversation also touches on the broader landscape of gene therapy delivery mechanisms and how advances are gradually expanding what's possible for more patients. Shifting gears, they critically examine recent vaccine policy developments and express concern about anti-scientific sentiment influencing public...
2025-06-1451 min
Flow StarsVirginia Litwin (International Society For Advancement of Cytometry)In this episode, Peter O'Toole is joined by Virginia Litwin, the President-Elect of the International Society For Advancement of Cytometry (ISAC).They discuss Virginia's journey from academia to industry and her pivotal role in the creation and development of CYTO Women, a committee that's having a genuine positive impact on the opportunities available for women in cytometry.She also shares her experiences in translational science, working closely with clinical trials, and her passion for standardization in flow cytometry. Watch or listen to all episodes of Flow Stars: flowstars.bitesizebio.com
2025-05-3055 minThe Conversational Flow - An Official Podcast of KCASImpactful Developments Across Oncology and Regulatory ScienceIn this milestone 20th episode of The Conversational Flow, hosts Adam and Brian dive into impactful developments across oncology, regulatory science, and bioanalytical innovation. The episode highlig4 hts a major Keytruda study showing the first significant improvement in head and neck squamous cell carcinoma outcomes in over two decades, the FDA’s pilot integration of generative AI in scientific review processes, and Sanofi’s acquisition of AI-developed bispecific antibodies for autoimmune diseases. The conversation rounds out with thoughtful discussion on data harmonization across multicenter flow cytometry studies and updates on KCAS’s scientific contributions at CYTO and regional events.“The Conversational Flow”...
2025-05-1453 minThe Conversational Flow - An Official Podcast of KCASBuilding on Backbone Panels: Standardization, Speed, and IntegrationIn the 19th episode of “The Conversational Flow”, our hosts, Brian and Adam, dive into the utility and innovation behind backbone panels in spectral flow cytometry, spotlighting a recent paper from BMS that presents a robust, flexible assay designed for immune monitoring using the Aurora platform. They discuss how backbone panels streamline assay development, enhance reproducibility, and are well-suited for integration with AI-driven gating strategies. The conversation also touches on industry trends shared at WRIB, including the push toward global harmonization of instruments and standards in clinical trials. With insights on panel optimization, autofluorescence extraction, and metadata standardization, the epis...
2025-04-141h 03
Flow StarsSpectral Flow Cytometry SpecialLet's shift our focus from just the people behind the science to the technologies they're using.In this special episode, we dive deep into spectral flow cytometry with a panel of leading experts, featuring:• Tamar Tak, Coordinator Flow Cytometry Facility, Leiden University Medical Center• Domenico Lo Tartaro, University of Modena and Reggio Emilia• Andrew Patentreger, Senior Team Lead, Broad Institute of MIT and Harvard• Kelly Lundsten, Biotechnology Market and Technical Bioassay Consultant, Luminous Bioanalytical Consulting• John Bianchi Colangeli, Resource Technologist I, Broad Institute of MIT and HarvardThey introduce the fundam...
2025-03-211h 06Royal Academy of Intl. Flow Cytometry AffairsBuy or Lease with First American (with Andrew Card and Nick Borkowski) | Tools of the TradeSend us a textTo Buy or to Lease? Finding funds for the purchase of flow cytometry equipment in your core facility can be challenging. In this first installment of the Tools of the Trade podcast, we discuss the leasing option offered by First American with Andrew Card and Nick Borkowski. RAIFCA's Tools of the Trade podcast highlights lesser-known tools available to flow cytometry core facility staff and users. We do not necessarily endorse the products discussed; our goal is to help listeners gain a better understanding of them.
2025-03-1955 minRoyal Academy of Intl. Flow Cytometry AffairsChatting Around A Whole New Dumpster Fire | The ChUG PodcastSend us a textAs much as I want to keep the ChUG podcast light and useful, we've entered a time where every piece of news we hear from the current administration is highly concerning. The media is discussing the stock market using stock images of people grabbing their face with their hands, we hear of NIH cuts and layoffs, and uncertainty is the only variable that is constant. I was happy to have Camille Stegen, Kathy Kluepfel, and Daniel Vocelle to discuss and provide some sense of clarity on how to move forward. So this is...
2025-03-191h 24The Conversational Flow - An Official Podcast of KCASFlow Cytometry & Cell Therapy: One Cell Can Make a World of DifferenceIn this engaging episode of the podcast, hosts Brian Wile and Adam Cotty welcome special guests Michael Naso, Hillary Quinn, and Barry Morse from The Therapeutic Innovations Group—a powerhouse team of scientists and biotech veterans with decades of experience across biologics, small molecules, and cell therapy. The conversation dives deep into the guests’ unique career journeys, and their own consulting venture aimed at helping startups and academic teams navigate early-stage biotech development. Along the way, listeners are treated to insider stories, genuine camaraderie, and valuable insights into cutting-edge topics like CAR-T cell analysis, flow cytometry, and the evolving land...
2025-03-141h 08The Conversational Flow - An Official Podcast of KCASFlow Validations and Guidance Document Changes in 2024-2025Episode 17 of The Conversational Flow podcast, hosted by Brian Wile and Adam Cotty, dives into the evolving landscape of flow cytometry networking and regulatory guidance, where they analyze recent FDA biomarker validation guidance, comparing it to existing industry standards like CLSI H62. Our hosts explore the challenges of harmonizing flow cytometry reporting, the role of regulatory clarity, and the potential for standardized data formats to drive the field forward. Packed with insights on professional networking and scientific validation, this most recent episode offers a deep dive into both career development and regulatory trends in flow cytometry.“The Co...
2025-02-1445 minThe Conversational Flow - An Official Podcast of KCASThe Role of Flow in Drug Autoimmune Indication ResearchIn episode 16 of The Conversational Flow podcast, hosts Brian Wile and Adam Cotty are joined by Allie Greenplate and Ayman Rezk to explore “The Role of Flow in Drug Autoimmune Indication Research.” The discussion delves into the surge in pharmaceutical R&D for immune and autoimmune indications, highlighting the influence of oncology treatments in shaping this trend. The guests shed light on how advancements in high-dimensional flow cytometry, particularly with more than 20-color capabilities, have accelerated breakthroughs in this field. They also discuss the cutting-edge frontiers of autoimmune research, from innovative therapies to emerging technologies in development. This episode prov...
2025-01-141h 00
Bloody MindedEpisode 28 - In the Lab: Flow Cytometry, Mass Spectrometry & Cytogenetics in MMCan’t get enough of obscure haematological lab chat, can you? Well we’ve got good news for you! Join us today as we delve even deeper into the arcane depths of flow cytometry, mass spectrometry and cytogenetics as they apply to myeloma. We promise we didn’t just make these words up.02:23 - Flow cytometry: overview04:01 - The flow cytometer: practical aspects09:01 - How we interpret flow11:44 - Myeloma flow markers14:32 - Quality in flow21:26 - Mass spectrometry25:59 - Cytogenetics27:46 - Cytogenetics assay principles30:30 - Karyotype abnormalities31:44 - FISH...
2024-12-1742 minThe Conversational Flow - An Official Podcast of KCASFantastic Fluorophores and Where to Find Them (w/ Kelly Lundsten)In this exciting 15th episode of The Conversational Flow podcast, titled "Fantastic Fluorophores and Where to Find Them," hosts Brian Wile and Adam Cotty from KCAS Bio are joined by special guest Kelly Lundsten from FluoroFinder. Together, they dive into the fascinating world of spectral flow cytometry, exploring key questions such as whether spectra or brightness is more critical for controls, which traditional fluorophores the field should consider moving away from, and what potential pitfalls new dyes might present for users. They’ll also discuss the operational benefits of adopting newer dyes, including how they can simplify cocktail preparation an...
2024-12-141h 05
That's What I Call Science!Episode 263: The Australasian Cytometry SocietyJoin us for this week’s episode, in which Olly and Georgia make their way through the poster session at the Australasian Cytometry Society’s annual 2024 conference! Cytometry may not be a word you’ve heard before, but is all about cells. And, by studying the cells in our bodies, the researchers in today’s episode have been making leaps and bounds in medical research in order to help the patients that need them.Show theme music: Kevin MacLeodHost: Olly Dove (@olly_dove)Co-Host: Georgia Stewart (@ggstew25)Prod...
2024-11-2427 minThe Conversational Flow - An Official Podcast of KCASModifying Flow Cytometry Panels: the “Backbone” of Flow CytometryA flow cytometry backbone panel is a set of antibodies that can be used as a starting point for identifying cell types, and then supplemented with additional markers. On the most recent episode of “The Conversational Flow”, our hosts, Brian and Adam, spend some time together as they answer the questions of “What is a backbone panel?”, “What type of changes should be considered for a backbone panel?”, “What are the advantages to backbone panels for translational teams?”, “What should one be careful of when modifying a backbone panel?”, “Should you use a backbone panel for a translational research program?”, and many ot...
2024-11-1450 minRoyal Academy of Intl. Flow Cytometry AffairsChUG podcast #21 - Meet Locally, Flow GloballySend us a textRegional flow cytometry are where it's at. This is what we have decided here at ChUG. We invited Aja Rieger to discuss the CoLD Summit, Karla Gorena to talk about FlowTex, and Daniel Vocelle to cover GLIIFCA. If you have unquestionably attended CYTO without any second look at other meetings, you've been missing out and this podcast is for you!Please note: David got pretty excited and dropped some expletives in the first minute of the podcast. Then he got a hold on himself. Edit 11/13/24: Karla Moncada Gorena is now D...
2024-11-131h 17The Conversational Flow - An Official Podcast of KCASLow Content Analysis: When a smaller panel is better than a larger oneDuring the most recent episode of “The Conversational Flow” podcast, our hosts Brian and Adam talk about those times when a smaller panel might be desirable over a larger panel. To dive deep into this discussion and really analyze all the angles of these questions, the guys have invited a special guest to join them – Amrutesh Puranik, PhD. Amrutesh is the founder of Bespoke MultiOmics Consultants, and his perspective is very insightful as they answer the questions of “What are the benefits of having a focused biological question and data interpretation plan?”, “Why are small panels recommended for cell sorting?”, “...
2024-10-141h 04The Conversational Flow - An Official Podcast of KCASHigh Content Analysis: Finding Needles in a Growing HaystackIn the 12th episode of “The Conversational Flow” podcast, Brian and Adam welcome two special guests – Sean Burke of Denovo Software and Ryan Brinkman of Dotmatics – who join them to discuss the answers to a number of specific questions about High Content Analysis: How can we manage and derive insight from higher complexity panels? How can this be standardized? Once standardized, how can the process be automated? Why is more data challenging to deal with? How can we generate meaningful answers from known and unknown antibody combinations? How can this enormous amount of data be standardized? How can this type of...
2024-09-141h 03The Conversational Flow - An Official Podcast of KCASGoing Global with Spectral Flow Cytometry - US, EU and Now AustraliaDuring this special episode of "The Conversational Flow" our hosts, Brian and Adam, welcome two guests: John Bucksath, CEO of KCAS Bio, and Stefan Cross, CEO of Crux Laboratories. These two guests discuss the elements and advantages of their organizations' new partnership between KCAS' US and European locations and Crux's location in Melbourne, Australia. Recent months have seen the three locations (Crux in Melbourne, and KCAS's Philadelphia and Lyon facilities) collaborate with Cytek to globalize the use of KCAS' multiple Cytek Aurora platforms and offer advanced spectral flow cytometry universally to their customers around the world. Learn about all...
2024-08-141h 06
Smart Biotech Scientist | Master Bioprocess CMC Development, Biologics Manufacturing & Scale-up, Cell Culture Innovation76: Transforming Single-Cell Analysis With Impedance Flow Cytometry w/ Furkan Gökçe - Part 2Curious about the latest innovations in cell analysis technology? Join us in this episode with Furkan Gökçe, New Business Development Manager at Amphasys, as we delve into the fascinating world of Impedance Flow Cytometry (IFC).Furkan shares how IFC is revolutionizing single-cell analysis by providing faster, more precise, and high-resolution data.Key Takeaways:Real-Time Precision: Discover how IFC allows for real-time, label-free assessments of cells, providing detailed insights into their natural state.Enhanced Process Control: Learn how IFC can detect early signs of nutrient deficiencies and contaminants, leading to timely interventions and im...
2024-08-0123 minSmart Biotech Scientist | Master Bioprocess CMC Development, Biologics Manufacturing & Scale-up, Cell Culture Innovation75: Transforming Single-Cell Analysis With Impedance Flow Cytometry w/ Furkan Gökçe - Part 1Ever wondered how single-cell analysis could revolutionize bioprocessing? Join us in this episode as we delve into the cutting-edge world of impedance flow cytometry.Furkan Gökçe, New Business Development Manager at Amphasys, shares his insights on the transformative power of this technology in the biotech industry. With a background in engineering and physics, Furkan provides a unique perspective on tackling the complexities of biological systems.Key Takeaways:Enhanced Process Control: Learn how impedance flow cytometry offers high-resolution, label-free analysis of individual cells, ensuring optimal growth conditions and quality production.Understanding Cell Heterogeneity: Di...
2024-07-3017 minThe Conversational Flow - An Official Podcast of KCASInstrument Comparison: Some Instruments are More Equal than OthersWhen it comes to flow cytometry instrumentation, there are a number of things that can be different. You may have different types of lasers, you might have a different path for the laser to follow to get to the detector, you can have different actual detectors, different filters in front of those detectors, not to mention the varying sensitivities or levels of power for any of these items. In episode #10 of "The Conversational Flow", Brian and Adam discuss all of these while comparing them and deciding which elements matter the most for your project.“The Conversational Flow” an o...
2024-07-1455 min
Flow StarsFlow Stars Recorded Live at CYTO 2024In this special episode of Flow Stars recorded live at CYTO 2024, our panel of esteemed guests, representing a cross-section of cytometry users, discuss the biggest matters in flow cytometry. They explore the capabilities of current cytometers and what future developments should focus on. They also consider the importance of making cytometry equipment more affordable and the level of technical support provided by manufacturers. Plus, the panel also discusses the importance of encouraging young researchers to use flow cytometry for small particle analysis and whether it's normal to name your instruments!With:• Cl...
2024-07-1034 min
BENCH to BEDSIDEExplore the Cores: Illuminating Discoveries With CHOP’s Flow Cytometry CoreIn the second part of our Explore the Cores series, Florin Tuluc, MD, PhD, Director of the Flow Cytometry Core Laboratory at CHOP Research Institute, sits down with host Susan Furth, MD, PhD, Chief Scientific Officer, to talk about what makes the Flow Lab a unique and valuable resource for the CHOP scientific community. Spoiler: It’s one-part state-of-the-art technology and two parts a team of incredible individuals with a passion for pediatric health.
2024-06-2815 min
Mentors at Your Benchside4 Fixatives for Histology and Cytometry: Perfect Your Preservation#108 — What should you use to fix your cells? Alcohols or aldehydes? Gluteraldehyde or formaldehyde? And how long will your cells stay fixed?This episode explains the four main fixatives for histology and cytometry and when to use them. It also provides some practical tips to ensure your fixation works and explains the benefits of combining fixatives. Check out the corresponding article for links to related resources. [1] To learn more about autofluorescence and the controls you need to check for it, read this article. [2]Resources1. 4 Fixatives for Histology and Cytometry. Perfect Your Preservation. Av...
2024-06-2506 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #20 - All the Flow Podcasts Are HereSend us a textIt was a pleasure to host Dr. Peter O'Toole, Director of the BioScience Technology Facility and Head of Imaging and Cytometry core at the University of York. He is also the host of the other, way more popular, flow cytometry dedicated podcast Flow Stars. We discussed all things podcasting, creating workshops and setting up meetings, and many more topics. We also discussed the RMS - visit www.rms.org.uk for more details and how to become a memberSubscribe to @BitesizeBio - home of Flow Stars and The Mic...
2024-06-241h 20Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #19 - In the Service of Cytometry: The ABRF 2024 SessionSend us a textThe Association of Biomolecular Resource Facilities (ABRF) had its 2024 annual meeting in Minneapolis and I was asked to chair the Service Contract Session. Pro tip: if that happens to you, you need to recruit Julie Auger, Executive Director at the Salk Institute, and she'll find equally great panelists for the session. It was a pleasure to have Brooke Bean Massani, Director of Research Support Services at the University of Arizona, and Roy Martin, Senior Field Marketing Manager for Biological Mass Spectrometry at Waters join the discussion. And for good measure, we had super...
2024-06-141h 16The Conversational Flow - An Official Podcast of KCASBatch Effects, Controls, and Considerations for Longitudinal StudiesLet’s face it - nobody likes batch effects (unless it has to do with cookies, but that’s for another podcast). In episode #9 of “The Conversational Flow”, Brian and Adam talk about Batch Effects which is when you're having changes in the study that are not necessarily related to the scientific variables of the study – and what that might mean you will need to do next. Batch Effects can cause a number of headaches for anyone without experience with them… luckily both of our hosts have a number of years in the lab, and they have seen a thing or two...
2024-06-141h 04The Conversational Flow - An Official Podcast of KCASReceptor Occupancy Testing in Clinical TrialsFor episode #8 of “The Conversational Flow” podcast, Brian and Adam spend the time discussing Receptor Occupancy Testing in Clinical Trials. Receptor occupancy (RO) assays are important in clinical trials because they provide valuable information about a drug’s binding characteristics and mechanism of action. Our hosts begin by outlining the general need for accurate RO calculation (per time point, per subject, etc), then they move on to pre-analytical risks (collection, shipment, transit, receipt, etc) and more specifics like staining and preparation process, acquisition and data analysis, harmonization between multiple testing facilities, and interpreting RO data.“The Conversational Flow” an...
2024-05-1445 min
Citeline PodcastsPodcast: Facilitating Patient Monitoring in CAR-T DevelopmentLearn how molecular technology is enhancing patient safety for CAR-T clinical trials.
US FDA recently initiated class safety labeling changes after evaluating the safety of CAR-T cancer treatment therapies. What does this mean for developers in the CAR-T landscape? Listen to your free podcast to find out.
Listen in on the conversation as experts from Cerba discuss facilitating patient safety measures in CAR-T therapies by accurately identifying the integration of viral vectors, utilizing flow cytometry, and more.
Listen now as experts discuss answers to:
· What patient monitoring is required by the FDA?
· What molecular technology does Cerba use and how do...
2024-04-2919 minThe Conversational Flow - An Official Podcast of KCASThe Current State of Receptor Occupancy ValidationFor episode #7 of “The Conversational Flow”, Brian and Adam invite David Ambrose to join them as they continue their conversation about Receptor Occupancy assays. There are so many unique considerations around an RO assay and with any flow cytometry assay, the precision of that assay is important. Our hosts discuss the biggest differentiators when you're starting to think about an RO assay and how to optimize the development of those assays and why it is so incredibly important to have a full understanding of the instrumentation being used in order to get the process right. “The Conversational Flow” an...
2024-04-151h 00Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #18 - Cezary and Daniel explain Spatial Biology to DavidSend us a textSpatial Biology is an emerging technology that can get a heck of lot confusing for David. When David doesn't understand something, he asks people to help. Be like David. Ask Cezary and Daniel to explains things to you.
2024-04-121h 19Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #17 - Chatting Around the Dumpster Fire III - Allegedly and PresumablySend us a textThe gang is back to discuss all kinds of things happening is Flowland! Ryan Duggan, Bert Ladd, Rebecca Bultema, and that other guy discuss which meetings look good this year, the range of ideas floating around reagents used to clean and maintain instruments, the problem of single stain controls when unmixing data, and careers outside of a flow core.
2024-03-281h 25
Marie Curie Science Research CenterFlow Cytometry By Dr. Marjan Assefi at the Forbes ScienceFlow Cytometry By Dr. Marjan Assefi at the Forbes Science
2024-03-2802 minThe Conversational Flow - An Official Podcast of KCASReceptor Occupancy: Deceptively Simple QuestionsThe newest episode of “The Conversational Flow” has Brian and Adam talking about the concepts around Receptor Occupancy and all the deceptively simple questions people often have about them, as well as the important but sometimes difficult answers to those questions. They talk about the biology of receptors and the few different "classes" associated with ROs. At their core, these are complex molecules with complex functions being discussed... and who better to have these complex discussions than our own experts, Brian and Adam?“The Conversational Flow” an official podcast of KCAS Bio, and focuses on the more niche to...
2024-03-1438 min
Mentors at Your Benchside7 Top Tips to Make the Most of Your Flow Cytometry Training#99 — So you’ve got your flow cytometry training booked and are one step closer to that precious data.But if you want to hit the ground running and get some useful data from your samples, there are some little things you'll need to do.These include reading up on a bit of background theory, understanding the capabilities of different types of cytometers, and thinking about what you want to learn from your experiment.In this episode of Mentors At Your Benchside, we've compiled cytometry training advice from a core facility manager to help you...
2024-02-2712 minThe Conversational Flow - An Official Podcast of KCASWhat Is Cell Sorting and What Role Does It Play in Bioanalysis?During this newest episode of “The Conversational Flow”, Brian and Adam discuss cell sorting and the pivotal role it plays in molecular biology applications – enabling researchers to isolate specific cell populations from complex mixtures, as well as a number of other benefits. Our hosts dive into the importance of cell sorting, the evolution of the field, and why KCAS Bio is the perfect partner for cell sorting in molecular biology applications. Using the right equipment and expertise for your cell-sorting can help you find and isolate the rare populations that have an outsized impact for your molecular biology analysis.
2024-02-1444 min
Listen In - Bitesize Bio Webinar AudiosBenefits of Imaging Data in Flow Cytometry Experiments: A Panel DiscussionExperience the transformative power of unbiased, data-driven cell analysis with access to over 25 image-derived label-free parameters, empowering you to easily assess sample quality, optimize gating strategies, and validate rare events.In this episode, learn how automated image analysis combined with flow cytometry can help improve data accuracy and precision, optimize and verify gating strategies, and discover novel biology, such as cell–cell interactions.Join a panel of experts as they discuss the features, benefits, and applications of a paradigm-shifting technology in flow cytometry with the Invitrogen™ Attune™ CytPix™ Flow Cytometer.Watch the full presenta...
2024-02-1241 minThe Conversational Flow - An Official Podcast of KCASWhat Makes Spectral Flow so Significant?For the 4th episode of “The Conversational Flow” podcast, Brian and Adam discuss spectral flow; something really near and dear to their hearts. This is a topic both of our hosts have been genuinely interested in since they really became aware of it, at which time they immediately began pushing to bring the instrumentation to KCAS. While they have hinted at it and talked about this topic off and on, even in just the few episodes of the podcast already, they take a significantly deeper dive in this episode and showcase why spectral flow is at the forefront of tech...
2024-01-1446 minThe Conversational Flow - An Official Podcast of KCASHow do You Validate a Flow Cytometry Assay?There are a number of different guidances for Flow Cytometric Assay Validation, so for episode #3 of “The Conversational Flow” podcast, Brian and Adam go into great detail on how we perform validations at KCAS, and under what context we do so – and why. In this episode, the guys break down the different types of validation and the various levels of qualification related to flow validation, as well as the differences between validation and qualification – which, in and of itself has has gone through an evolution as a concept – and discuss how this all influences how to validate a flow cytometry...
2023-12-1443 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #16 - In the Service of Cytometry with Ray LanniganSend us a textWe continuing our exploration of service contracts in the Flow Cytometry with none other than Ray Lannigan, formerly Vice President of Global Sales and Services at Cytek. We couldn't ask for a better guide to explain the details of service contracts form the industry point of view. Here we discuss how service contracts are priced, what kind of leverage is available to negotiate with manufacturers, the other options for those who may want to look elsewhere for service, tracking service on your instruments, rights to repairs, and many other topics.
2023-12-121h 34Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #15: Spectral Flow - What You Don't Know Is Out To Kill YouSend us a text'What you don't know is out to kill you' had been the subtitle for the CAT Facility flow basics class for over a decade. It reminds us that the life of a flow cytometrist is filled with peril and danger. One day you're on the top of the world, thinking you know everything about spectral flow. Then some company comes out with a bunch of marketing material and data that make you question all that you ever hold dear. So we invited Ryan Duggan, Dave Adams and Rachael Sheridan to discuss what's going...
2023-12-1259 minThe Conversational Flow - An Official Podcast of KCASHow Sample Collection Impacts Flow CytometryFor our second episode of “The Conversational Flow” podcast, Brian and Adam felt that the best topic to discuss was that of sample collection, sample processing and the important role it plays in Flow Cytometry analysis. This is an often discussed topic – What is the best way to collect and process samples – and it really comes down how you plan to use the samples. When looking at certain assays, fresh samples are needed, whereas with others, frozen samples may not only be acceptable – they may even be preferable. Brian and Adam dive into this episode with all the info you will n...
2023-11-1456 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #14: Cytometry on Air #3: Cell SortingSend us a textRyan ran a discussion on the cell sorters available on the market on the Cytometry on Air podcast, roughly 10 years ago. It's now 2023 and we invited Dave Adams back along with Bill Eades to discuss how things look nowadays. We talked about the premature deployment of cell sorters, ultra-specialization of platforms, and planned obsolescence. We covered the most popular droplet-based platforms available, as well as the microfluidics systems.
2023-10-141h 23The Conversational Flow - An Official Podcast of KCASWhy Does Flow Cytometry Need Its Own Podcast?Flow cytometry is a technique used to detect and measure physical and physiological characteristics of a population of cells or particles. Tens of thousands of cells can be quickly examined and the data gathered is processed by a computer. Uses of flow cytometry can included determination of Cellular characteristics and function, biomarker detection, proliferation, toxicology and diagnosis of health disorders. For our inaugural episode of "The Conversational Flow", Adam and Brian discuss how Flow Cytometry has become so important to the industry and why it warrants getting its very own podcast."The Conversational Flow" an official podcast...
2023-10-1340 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #13 - In the Service of Cytometry (with Tony Leger)Send us a textWe're thrilled to host Tony Leger, Director of Automation Laboratory Technology (ALT), for a discussion on instrument service in the field of Flow Cytometry. If you have frustrations regarding the cost of service contracts, or the difficulty around repairing your own instruments, this discussion should provide some great insights. We're hoping to expand the 'In the Service of Cytoemtry ' series to multiple actors in the field to better understand what is going on, but we wanted to start discussion to begin with the voice of Tony Leger. His care for right to...
2023-09-192h 11
Flow StarsLive at CYTO 2023 with Jessica Houston, Pratip Chattopadhyay, and Paul Wallace#S4:E1 — In this special episode of Flow Stars recorded live at CYTO 2023, host Peter O'Toole welcomes a panel of distinguished experts, including Jessica Houston (New Mexico State University), Pratip Chattopadhyay (NYU Langone Health (PIL)), and Paul Wallace (Roswell Park Cancer Comprehensive Center).Join them for an enlightening conversation as they dive into the future of flow cytometry and provide their unique perspectives on how the utilization of flow cytometry could evolve in the next 5-10 years.But that's not all! Our guests share their favorite lab tools, revealing a world beyond cytometers. They reveal wh...
2023-09-0636 min
Venture In The SouthE82: Nirvana Sciences Innovates on Flow CytometryNirvana Sciences has innovated traditional flow cytometry, a longstanding cellular diagnostic tool, in a unique and powerful way to dramatically increase the efficiency and utility of this tool for a wide range of diagnostic problems. Through development of proprietary bright narrowband fluorescent markers (or dyes), NS has enabled a magnitude increase in the number, fidelity and efficiency of cell markers that can be measured from a single sample. This has positioned NS with strong IP for an acquisition by a major diagnostics company.Follow David and Paul: https://x.com/DGRollingSouth ...
2023-09-0427 min
Listen In - Bitesize Bio Webinar AudiosGarbage In, Garbage Out: Sample Prep for Flow CytometryWhat's more disappointing than running your flow cytometry sample to discover it's rubbish? Especially considering time on cytometers can be precious, even more so if you've booked onto an in-demand one at a busy core facility. You've done all the work and all the waiting—for nothing. If this scenario sounds familiar, this episode of Listen In is for you.Jessica Rowley, Flow Cytometry Facility Manager at Imperial College London, explains how to prepare flow samples optimized for your experiments, to save you time and avoid disappointment.She has worked with hu...
2023-08-281h 00
CAPcast from the College of American PathologistsCAP Author Chat - The Color Atlas of Flow CytometryJust released, the new Color Atlas of Flow Cytometry offers a full range of hematolymphoid disease cases, highlighting disease categories and disease entities that may be difficult to diagnose in clinical practice. The new book can benefit students, medical technologists, and practicing pathologists, explains lead author, David Dorfman, MD, PhD, FCAP in this CAPcast interview.
To purchase this book, please visit CAP's estore: https://estore.cap.org/OA_HTML/xxCAPibeCCtpItmDspRte.jsp?section=10044&item=747354&sitex=10020:22372:US
2023-04-2107 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #12 - Imaging Flow Cytometry. What's going on?Send us a textWe're back! We wanted to make sense of the numerous imaging flow cytometers that have hit the market over the last few years, notably the Attune CytPix and the BD FACS Discover S8. Ryan Duggan, Director Emeritus Pat Simms, Daniel Vocelle and that other guy discuss the potential use of the technology, and harp on an imaginary Image Stream Cell sorter.
2023-03-131h 15
Laboratory Considerations for Clinical TrialsFlow Cytometry as an IVD technology for Companion DiagnosticsIn this episode, Megan McCausland, Scientific Advisor for Flow Cytometry at Q2 Solutions, and Scott Bornheimer, Associate Director of Medical and Scientific Affairs at BD Biosciences talk about the enormous potential of flow cytometry as a clinical diagnostic tool.
Currently, pharma companies are looking for simplified, standardized flow cytometry tests to help with enrolling patients and understanding outcomes in their studies.
In the longer term, Megan McCausland notes what will lead to the use of flow as a CDx:
“I think the drug development landscape really continues to evolve towards more personalized medicine. As t...
2022-11-3020 min
Dermasphere - The Dermatology Podcast87. Flow cytometry with Dr. Vermeer - Ruxolitinib cream for vitiligo - Baricitinib for AA - Roflumilast for psoriasis - Tapinarof for psoriasisFlow cytometry with Dr. Vermeer -
Ruxolitinib cream for vitiligo -
Baricitinib for AA -
Roflumilast for psoriasis -
Tapinarof for psoriasis -
Connect with Dr. Vermeer!
https://www.universiteitleiden.nl/en/staffmembers/maarten-vermeer#tab-1
Luke's Practical Dermatology for Primary Care course: https://registration.socio.events/e/pdpc2022
Michelle and Luke will both be speaking at the upcoming Texas Derm Society meeting! https://www.texasdermatology.org/amsimis/TDS/Meetings/Annual_Meetings/Annua_Meetings.aspx
Connect with us!
Web: https://dermaspherepodcast...
2022-09-051h 01
Listen In - Bitesize Bio Webinar AudiosIncorporating New Dyes to Simplify Panel Design/ReagentIncorporating New Dyes to Simplify Panel Design: Accelerating Reagent AvailabilityIn this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components.We will cover:How quality custom reagents in small sizes will make your panel design easierHow you can receive these reagents fast and without traditional wait times for manufacturingHow you can be confident that quality control is maintained for these custom reagentsIncorporating New Dyes to Simplify Panel DesignDr. Jorg Ruhrer, Director of...
2022-08-3141 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #11 - 2022 in ReviewSend us a textHere at ChUG in Cytometry, we aim to be the most relevant that we can be! This is why we decided to comment on events that have not happened yet! Laura Johnston, Pat Simms, Robot Ryan Duggan and David Leclerc discuss upcoming flow meetings, what's going on at ISAC, cells sorters, David is shilling for industry, and hiring new staff in SRLs. We also say goodbye to Laura who is moving to back to Seattle.
2022-04-191h 21
Future Science GroupFlow cytometry for CGT assay development: an interview with Richard CuthbertFlow cytometry for CGT assay development: an interview with Richard Cuthbert by Taylor & Francis Group
2022-04-0511 min
Unravelling Science PodcastEpisode 47: Dr Barry Moran: Flow Cytometry, Part Time PhD & Work/Life BalanceDr Barry Moran core facility manager of the Flow Cytometry Facility, at the Trinity Biomedical Sciences Institute is my guest this week. He chats to me about his 'unconventional' journey through academia, deciding to do a part time PhD while running the Cytometry core facility and why the 'journey-man' in him may be relatable.
Barry also discusses how flow cytometry can advance our scientific knowledge and its benefits particularly in the context of immunology. He describes his own research examining immune dysfunction in the skin disease hidradenitis suppurativa and why this is research that adds value to p...
2022-02-0853 min
The Fellow on Call: The Heme/Onc PodcastEpisode 002: Heme Path Series, Pt 1 - Flow CytometryIn Part 1 of our Heme Path series, we break down the logistics and applications of flow cytometry.Episode contents:- What is flow cytometry?- How do we use this to identify cells?****Have some time and want to make some extra money? Get paid to participate in market research surveys: https://affiliatepanel.members-only.online/FOC_24?utm_campaign=FOC&utm_source=email&utm_medium=email** Want to review the show notes for this episode and others? Check out our website: https://www.thefellowoncall.com/our-episodesLove what...
2022-01-1900 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #10 - The Flood of '21Send us a textThe CAT Facility lost a few instruments due to some water damage a few months ago and we turned to an Insurance Group to help us out. It's not a common situation for anyone (hopefully), so here's some thoughts on our experience and what can be done to avoid it as much as possible.
2021-11-1722 minRoyal Academy of Intl. Flow Cytometry AffairsChUG Podcast #9 - Chatting Around the Dumpster FireSend us a textThe gang is back! Pat Simms, Laura Johnston, Ryan Duggan, and that other guy discuss the latest news in the flow cytometry world in this second edition of Chatting Around the Dumpster Fire. They talk about hiring staff, getting back to work in the COVID era, creating online content in flow, the great floods in history, the Quanteon, CYTO 2021 (it seems they didn't see much of it), and the future of cytometry, both spectral and mass.
2021-08-241h 35Royal Academy of Intl. Flow Cytometry AffairsChUG #8 - Dealing with autofluorescence in spectral flow cytometrySend us a textWe host Monica Delay, US Manager for Technical Application Support at Cytek Biosciences, for a discussion on autofluorescence (AF) and the tools to deal with it in Spectral Flow Cytometry. We use figures to showcase some problems with AF which will be missing from this audio version of our talk. You can visit our website at Chugcytometry.com to find the video, along with a list of links to webinars and publications dealing with AF.
2021-07-191h 22
MDforLivesClinical flow cytometry in cancer diagnosis and immune system disordersThis episode is also available as a blog post: https://mdforlives.blog/2021/06/21/flow-cytometry-in-cancer-diagnosis-and-immune-system-disorders/
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Send in a voice message: https://anchor.fm/mdforlives/message
2021-06-2105 minRoyal Academy of Intl. Flow Cytometry AffairsChUG #7 - There is no I in 'cell sorter development'Send us a textOur guest is our very good friend James McCracken. We explore his career path from his post-doc at The University of Chicago, to becoming the Core Facility Director of the University of Louisville Diabetes and Obesity Center, to his current position as the Commercial Marketing Manager with Beckman Coulter Life Sciences. He has been deeply involved in the development of the newly released CytoFlex SRT cell sorter, so we also discuss the ins and outs of flow cytometry instrumentation development in industry.
2021-06-141h 06Royal Academy of Intl. Flow Cytometry AffairsChUG #6 - Considerations on Online TrainingSend us a textOur guests this week are Kathy Daniels, Derek Davies, and Rui Gardner. You will recognize them from the work they have done in training Flow Cytometry users over the year. They have also developed an impressive amount of online content during the pandemic, including the OpenFlow Cytometry series available on YouTube. We sat down to discuss the ins and outs of online training, the opportunities and pitfalls that we have experienced, and some of the features that could stay with us once we are back in a normal environment.
2021-05-111h 14
Flow StarsTim Bushnell (URMC) & Alfonso Blanco (UCD)#S1:E1 — Our first episode of Flow Stars features not one, but two big hitters in the world of flow cytometry, which creates a dynamic and energetic conversation. We find out about Tim’s love for brewing, including how he taught his kid to brew as a science lesson and discuss Alfonso’s passion for kicking people (in organized sports) and how his Olympic hopes were dashed by an unfortunate knee injury.While we get to know Tim and Alfonso on a personal level, work and flow cytometry is still a big focus, with both sharin...
2021-05-0555 min
iGEM Foundation ChannelWeek #6A: Quantifying fluorescence and cell phenotypes with flow cytometry 1/3More info: https://2020.igem.org/Measurement/Webinars
This webinar begins with an introduction to flow cytometry, including how these instruments operate and the types of data that they produce. The second block of the webinar focuses on calibration of measurements for fluorescence and cell size, and on debugging such measurements. Finally, we will end this session with a discussion of how to interpret and debug calibrated flow cytometry data.
Part 1/3: https://youtu.be/ODF-9L2FLbg
Part 2/3: https://youtu.be/6jCxWe9QsUM
Part 3/3: https://youtu.be/WOhPj5Csog0
2021-04-1700 miniGEM Foundation ChannelWeek #6A: Quantifying fluorescence and cell phenotypes with flow cytometry 3/3More info: https://2020.igem.org/Measurement/Webinars
This webinar begins with an introduction to flow cytometry, including how these instruments operate and the types of data that they produce. The second block of the webinar focuses on calibration of measurements for fluorescence and cell size, and on debugging such measurements. Finally, we will end this session with a discussion of how to interpret and debug calibrated flow cytometry data.
Part 1/3: https://youtu.be/ODF-9L2FLbg
Part 2/3: https://youtu.be/6jCxWe9QsUM
Part 3/3: https://youtu.be/WOhPj5Csog0
2021-04-1700 minRoyal Academy of Intl. Flow Cytometry AffairsChUG #5 - Meeting Julie AugerSend us a textThis is a reunion episode of kind! Our guest is Julie Auger, Executive Director of the RCFP at UCDavis, but also the architect of the Office of Shared Research Facilities at the UoC. And the co-founder of ChUG itself!! It a pleasure to host Julie as we discuss the early days of the OSRF, and the model of centralized administration for core facilities.Visit us at chugcytometry.com for more discussions.
2021-04-141h 15Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #4 - Meeting Pratip ChattopadhyaySend us a textIn this episode, we sit with Dr. Pratip Chattopadhyay, Associate Professor in the Department of Pathology and Director of the Applied Immunology Core at NYU Grossman School of Medicine, to discuss his paradigm breaking work in flow cytometry, immunology and insights into career development for us all.Visit us at ChUGCytometry.com for more discussions.
2021-03-161h 18Royal Academy of Intl. Flow Cytometry AffairsChUG Podcast #3 - The Once and Future Flow Cytometry Analysis ToolsSend us a textWe’re hosting President and Founder of De Novo Software, David Novo, and Cytek Application Scientist, Geoff Kraker, to discuss analysis software tools from turn of the century to present day.
2021-02-131h 27
SciPodExploring Symbiotic Relationships Using Flow Cytometry - Dr Toshiyuki Takahashi, National Institute of Technology in Miyazaki, JapanSymbiosis is the interaction between two species that offers benefits to one or both of the organisms involved. One important type of symbiosis that has been instrumental in advancing evolution is endosymbiosis, where one of the organisms lives inside the other. Endosymbiosis has typically been studied using microscopy, but Dr Toshiyuki Takahashi from the National Institute of Technology in Miyazaki, Japan, proposes that a technique called flow cytometry can offer more detailed insights into endosymbiotic relationships and advance our understanding of these important associations.
2020-08-0708 min
The Lung Science Podcast: An AJRCMB PodcastEpisode 13: Flow CytometryDr. Andrea Yu sits down with Dr. Robert Tighe to discuss everything you wanted to know about flow cytometry (but were afraid to ask).
2020-05-0731 min
HumAIn PodcastMachine Learning with R, the tidyverse, and mlr by Hefin Rhys[Audio] Podcast: Play in new window | DownloadSubscribe: Google Podcasts | Spotify | Stitcher | TuneIn | RSSHefin Rhys is a Senior Scientist (flow cytometry) at UCB. He completed his PhD at the William Harvey Research Institute in Queen Mary University of London in 2017, and graduated from my MPharmacol degree from the University of Bath in 2013. His main academic interests are conventional, imaging and small particle flow cytometry, data science and machine learning. Episode Links: Hefin Rhys’ LinkedIn: https://www.linkedin.com/in/hefin-rhys/ Hefin Rhys’ Twitter: @HRJ21Hefin Rhys...
2020-04-0246 min
CytoFluo@ICGEB 2019A. Zucchetto - Flow cytometry for basic research and diagnosis of lymphoid malignanciesAntonella Zucchetto, CRO Aviano, Italy speaks on "Flow cytometry for basic research and diagnosis of lymphoid malignancies". This movie is part of "Flow cytometry: from basic principles to advanced application" Course, 8-10 May 2019, ICGEB Trieste, Italy.
2019-08-2632 minCytoFluo@ICGEB 2019E. Lugli - High content flow cytometry to dissect memory T cells responsesEnrico Lugli, Humanitas Milano, Italy, speaks on "High content flow cytometry to dissect memory T cells responses". This movie is part of "Flow cytometry: from basic principles to advanced application" Course, 8-10 May 2019, ICGEB Trieste, Italy.
2019-08-2649 minCytoFluo@ICGEB 2019S. Arioli - Flow cytometry applications in Food Microbiology: not only cell counting!Stefania Arioli, UniMilano, Italy, speaks on "Flow cytometry applications in Food Microbiology: not only cell counting!". This movie is part of "Flow cytometry: from basic principles to advanced application" Course, 8-10 May 2019, ICGEB Trieste, Italy.
2019-08-2653 min
The Stem Cell PodcastEp. 147: “Targeted Repair in Blood Stem and Progenitor Cells” Featuring Dr. Jennifer AdairGuest:
Dr. Jennifer Adair is an Assistant Member in the Clinical Research Division at Fred Hutchinson Cancer Research Center. Her lab uses a combination of cell biology, molecular biology, chemistry, engineering, nanomedicine and bioinformatics to develop new gene therapy treatments for many different diseases, including cancer. Her goal is to develop safe, cost-effective and clinically relevant applications for gene therapy that can be implemented worldwide.
Featured Products and Resources:
Science in Seattle
StemSpan SFEM II
Resources and Links
Possible Drug Target for Heart Condition Identified - Researchers have found that a genetic mutation linked to dilated cardiomyopathy activates a signaling...
2019-07-231h 23
Just ScienceJust Imaging Flow Cytometry_2019 NIJ R&D_108In episode ten of the 2019 R&D season, Just Science interviews Dr. Christopher Ehrhardt, professor at Virginia Commonwealth University, about a method for determining tissue type, age of evidence, and contributors from biological mixtures using cellular autofluorescence signatures.
It goes without saying that cells collected from different parts of the body look different. Buccal, vaginal, epidermal, and blood cells all have unique intrinsic properties. However, when they are combined, it can be difficult to discern what components are actually in the mixture. Using Imaging Flow Cytometry, Dr. Ehrhardt has found a way to differentiate between cell types, estimate cellular age...
2019-07-2234 min
iBiology Videos Without SubtitlesSusanne Heck: Mass CytometryDr. Susanne Heck begins her talk by explaining why we might choose to use mass cytometry rather than other types of flow cytometry. Traditional flow cytometry is typically limited to the detection of about a dozen parameters in one sample due to overlap between the emission spectra of fluorochromes used to label antibodies. Mass cytometry, on the other hand, allows for the detection of up to 50 parameters in one sample because antibodies are labelled with metal isotopes and separated based on their mass. Heck goes on to explain which metal isotopes are typically used for mass cytometry and why, and sh...
2019-06-3038 min
Future Science GroupExosomes in flow cytometry: an interview with Joyce SlusserFind out more about the role of exosomes in flow cytometry studies in this interview Joyce Slusser (KCAS; KS, USA).
2019-05-0108 min
Future Science GroupFlow cytometry: an interview with Clara Brando and Ted BaginskiFind out more about current challenges and recent developments in seen in the field of flow cytometry in this interview with Clara Brando and Ted Baginski (Eurofins; MO, USA).
2019-05-0119 min
PhD Transition Report On-DemandWhat PhDs Need To Know About Leadership & How To Become A Career Multiplier
Skip ahead to:
05:40 – Show Me the Data23:35 – What PhDs Need To Know About Leadership w/ Liz Wiseman44:20 – Importance Of Continued Technical Training w/ Tim Bushnell, Ph.D.56:20 – Project Manager Career Track w/ Orly Levitan, Ph.D.
Do want to move beyond entry level positions?
Ready to learn more about how you can develop your leadership skills?
In this episode of Cheeky Scientist Radio we are very excited to be joined by Liz Wiseman, renowned Researcher, Executive Advisor, CEO and Author. As a PhD you are qualified for management-level positions...
2019-03-291h 13Laboratory Considerations for Clinical TrialsMonitoring the Tumor Micro-environment with Flow Cytometry - Mark EdingerMark Edinger is the Scientific Advisor for Flow Cytometry at Q2 Solutions. In this episode, he describes recent advances in flow cytometry and what that means for immuno-oncology trials.
New instruments, reagents and software are enabling researchers to monitor 30-some markers simultaneously to get a better picture of the tumor micro-environment and the interactions taking place.
For example, there's a whole list of new checkpoint inhibitors, like PD-1, CTLA-4, CD-1-52 and a multitude of other CD-47 that have been described, and their ligands on T cells and immune cells, not just T cells, that...
2019-03-0520 min
Anna & Lue?????????????Initial Diagnostic Workup of Acute Leukemia GuidelineThe purpose of this podcast is to educate and to inform. This is not a substitute for professional medical care and is not intended for use in the diagnosis or treatment of individual conditions. Guests on this podcast express their own opinions, experience, and conclusions. The mention of any product, service, organization, activity, or therapy should not be construed as an ASCO endorsement.
[MUSIC PLAYING]
Hello and welcome to the ASCO Guidelines podcast series. My name is Shannon McKernin and today I'm interviewing Dr. Valerie de Haas from Princess Máxima Center for Pediatric Oncology in the Netherlands, lead author o...
2018-12-0312 min
Future Science GroupFlow cytometry: a podcast with Christoph EberleFind out more about the flow cytometry in this Bioanalysis Zone interview with Christoph Eberle (Charles River Laboratories), as part of our 'In the Zone' feature. You can find out more about flow cytometry on the website, at: http://bit.ly/2RuwAKi
2018-10-0813 min
VJHemOnc PodcastAcute leukemias: is MRD already a surrogate for survival?The use of minimal residual disease (MRD) in the management of acute leukemias is a hotly debated topic. Despite the potential of MRD status as a prognostic marker and measure of disease, there are a number of controversies surrounding its use. These were discussed in a thought-provoking session at the 1st International Workshop on Acute Leukemias (iwAL), held in St. Pete Beach, FL. Chairing the discussion, Farhad Ravandi, MD, of the University of Texas MD Anderson Cancer Center, Houston, TX, is joined by Marion Subklewe, MD, of LMU-University Hospital Munich, Munich, Germany, and Adriano Venditti, MD, University of Rome...
2018-03-0914 min
Brave Your FearChaquetta Grace – Alpha Woman, Scientist, Bride#10 Episode with Chaquetta Grace – Alpha Woman Scientist Bride FEB 14, 2018 Chaquetta Grace 2004 Atlanta, GA I am a servant. I am a scientist. I am a sister. BE better. Badass Quotes: “I knew that I was pretty good, but I wasn’t phenomenal.” (3:15) “I knew what my potential was.” (10:43) “I had no hesitation. I had no hesitation.” (18:59) “It was stepping out of that comfort zone because I knew I was destined for more.” (39:25) “It becomes this fire within your gut, and a need.” (40:14) “I feel l...
2018-02-1454 min
St Edmund Hall Research Expo 2015: Teddy TalksColouring-in for AdultsHow flow cytometry can help investigations into immune-mediated diseases. Flow cytometry is a technique which uses flourescent compounds of different colours to label molecules on cells. Using this approach we can investigate the cell populations responsible for causing immune-mediated diseases such as Ankylosing Spondylitis and explore how new therapies can alter the behaviour of the different cell types.
2015-06-1111 minSt Edmund Hall Research Expo 2015: Teddy TalksColouring-in for AdultsHow flow cytometry can help investigations into immune-mediated diseases. Flow cytometry is a technique which uses flourescent compounds of different colours to label molecules on cells. Using this approach we can investigate the cell populations responsible for causing immune-mediated diseases such as Ankylosing Spondylitis and explore how new therapies can alter the behaviour of the different cell types.
2015-06-1111 min
Graduate School of Systemic Neurosciences - Digitale Hochschulschriften der LMUAnti-neurofascin antibodiesNeurofascin (NF) is a cell-adhesion molecule that is found at the nodes of Ranvier. The 186
kDa isoform of neurofascin (NF186) is expressed on the axon in the exposed node, and the
155 kDa isoform (NF155) is expressed on myelinating glia at the paranode. NF186 is essential
for clustering of sodium channels to the nodes while NF155 is needed for close paranodal interactions
between myelinating glia and axons. The neurofascins are found in both the peripheral
and central nervous system (PNS and CNS).
NF-specific autoantibodies were identified in serum of multiple sclerosis (MS) patients using
a proteomics approach with two-dimensional Western blotting...
2012-11-2000 min![[Audio] BME 695L: Engineering Nanomedical Systems](https://nanohub.org/site/resources/2011/08/11877/itunes_audio.png)
[Audio] BME 695L: Engineering Nanomedical SystemsBME 695L Lecture 14: Designing and Testing Integrated Nanomedical SystemsSee references below for related reading.14.1 Introduction to integrated designs14.1.1 “Total design” but there is some order in the design process14.1.2 A brief outline of the total design process14.2 Choose autonomous or non-autonomous design14.2.1 If autonomous, will there be error-checking to correct mistargeting?14.2.2 If autonomous, does the NMS perform all of the multi-step process sufficiently to accomplish the objective?14.2.3 If non-autonomous, what form of external modulation of the in-vivo nanomedical system will be used?14.2.4 If non-autonomous, are the external interactions able to adequately control the actions of the NMS?14.2.5 Evaluate reaction of NMS to external intervention14.2.6 Compare actions of NMS with and without external intervention.14.2.7 ...
2011-11-2300 min[Audio] BME 695L: Engineering Nanomedical SystemsBME 695L Lecture 14: Designing and Testing Integrated Nanomedical SystemsSee references below for related reading.14.1 Introduction to integrated designs14.1.1 “Total design” but there is some order in the design process14.1.2 A brief outline of the total design process14.2 Choose autonomous or non-autonomous design14.2.1 If autonomous, will there be error-checking to correct mistargeting?14.2.2 If autonomous, does the NMS perform all of the multi-step process sufficiently to accomplish the objective?14.2.3 If non-autonomous, what form of external modulation of the in-vivo nanomedical system will be used?14.2.4 If non-autonomous, are the external interactions able to adequately control the actions of the NMS?14.2.5 Evaluate reaction of NMS to external intervention14.2.6 Compare actions of NMS with and without external intervention.14.2.7 ...
2011-11-2300 min