Shows
Listen In - Bitesize Bio Webinar AudiosInside Immunoassays: Strategies to Maximize Your Immunoassay Data and InsightsImmunoassays are pivotal in scientific research, enabling the precise identification and measurement of specific analytes. They pave the way for investigations into disease mechanisms, discovering biomarkers, and evaluating the efficacy of potential therapeutic interventions.The key reagents included in an immunoassay can significantly impact reliability and reproducibility, underscoring the importance of understanding critical components within immunoassay kits.When selecting an immunoassay platform, it's essential to consider critical elements such as multiplexing capabilities and sensitivity needs as part of the experimental design.Additionally, understanding the various assay formats and their data outputs can aid...2025-04-2837 min
Listen In - Bitesize Bio Webinar AudiosAI-Powered 3D Organoid AnalysisMulticolor 3D fluorescent confocal microscopy presents significant challenges in image acquisition and data analysis. However, AI is making a massive impact on 3D spatial biology. This episode of Listen In explores the incorporation of AIVIA, an AI-powered image analysis software, into workflows to address these challenges in analyzing multicolor fluorescent microscopy data of 3D organoids. See how AIVIA’s tools enhance the visualization and quantification of cellular structures and organoids, enabling precise segmentation and analysis of fluorescent signals. And get a showcase of how AIVIA supports Python-based image analysis for microscopists. Wa...2025-03-311h 02
Listen In - Bitesize Bio Webinar AudiosVirtual Reality Showcase for Stellaris: Leica’s Confocal Microscopy PlatformJoin Advanced Workflow Specialists for a virtual reality showcase of Leica's confocal microscopy platform, STELLARIS. In this episode of Listen In, discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously.Watch the full presentation here: https://microscopyfocus.com/confocal-microscopy-stellaris-showcase/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/2024-10-1425 min
Listen In - Bitesize Bio Webinar AudiosMultiplex Annotated Tissue Imaging System (MANTIS®)Join us on Listen In as we unveil a breakthrough method for imaging skin immune cells, crucial for accurate diagnoses and personalized treatments. Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with the MANTIS system.Learn how this innovative approach allows for the acquisition of up to 10 colors, providing detailed immune phenotyping of skin samples. Plus, discover how MANTIS simplifies complex imaging, making advanced analysis accessible and practical for routine clinical practice.Tune in to explore...2024-09-301h 07
Listen In - Bitesize Bio Webinar AudiosFive-Color STED With a Single Depletion Laser and Fluorescence Lifetime Phasor SeparationDive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In. Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching.Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels....2024-09-1656 min
Listen In - Bitesize Bio Webinar AudiosFinding The Ideal Hook for Your Science StoryYour research is important, but it can be hard to explain to others why it's important. In this episode of Listen In, you'll learn to find the ideal hook for your science story. This trick, borrowed from journalism, involves taking a step back and thinking about all the aspects of your research and where they might overlap with other topics that attract interest from other people. Knowing how your research connects with other areas is useful when writing grant proposals or giving public talks about your work because it helps you write or...2024-07-0837 min
Listen In - Bitesize Bio Webinar AudiosCryo-tomography to Volume EM: Explore with Hydra Bio Plasma-FIBVolume electron microscopy or volume EM (vEM) was named one of Nature's Technologies to Watch in 2023. The new Thermo Scientific Hydra Bio PFIB integrates advanced cryo-TEM lamella preparation to volume EM workflows.In this episode of Listen In, see how the new Thermo Scientific Hydra Bio Plasma-Focused Ion Beam (PFIB) extends cryo technology to volume EM and enables the acquisition of high-resolution 3D data through automated serial milling and imaging of frozen-hydrated specimens.Cryo Auto Slice & View enables the acquisition of high-resolution 3D data through automated serial milling and imaging for striking new insights into...2024-05-131h 00
Listen In - Bitesize Bio Webinar AudiosElevate Your Research with Spatial InsightsIn this episode of Listen In, learn more about how the convergence of AI technology and scientific discovery can revolutionize our understanding of biology and disease. Generating insights into tissue microenvironments is crucial to our understanding of normal and abnormal tissue development, such as during cancer progression. Spatial biology methods using microscopy and multiplexing techniques have allowed researchers to bring together protein expression profiles and spatial information to dive into the complex biology needed for these insights. However, image analysis of these datasets, especially with high numbers of protein markers and/or large numbers of...2024-04-2957 min
Listen In - Bitesize Bio Webinar AudiosUnleash New Biological Insights with Innovative GlycoanalyticsGlycosylation is a prevalent modification of proteins known to play diverse roles across a wide range of biological processes. While glycoproteins are widely recognized as key molecular features involved in human health and disease, the analysis of protein glycosylation has historically been hampered by a lack of efficient and simple methods to characterize the analytically challenging glycan structures. Examples include structures exhibiting extensive heterogeneity and subtle isomeric features such as linkage and topological differences. In this episode of Listen In, Dr. Thaysen-Andersen discusses opportunities and challenges in the burgeoning field of analytical glycoscience. ...2024-04-2253 min
Listen In - Bitesize Bio Webinar AudiosContamination and Recovery of PFAS in Analytical Methods Requiring Filter MembranesLiquid chromatography-mass spectrometry (LC-MS) is a crucial technique in many life sciences, including proteomics, metabolomics, pharmaceutical analysis, clinical diagnosis, and forensic science.In this episode of Listen In, elevate your analysis and liquid chromatography. Learn what consumables, materials, and reagents can harm your LC-MS data for more accurate quantification using perfluoroalkyl substances (PFAS) as an example.See how contamination from consumables, equipment, reagents, and non-specific binding of samples to syringe filters can make LC-MS quantification inaccurate. Plus, explore the environmental impact of PFAS and how to choose syringe filters and other consumables for...2024-04-1549 min
Listen In - Bitesize Bio Webinar AudiosLabel-free 3D Live Cell Imaging and Quantification using HolotomographyHolotomography has emerged as a helpful tool for imaging live specimens without additional pre-treatment, such as fixation, fluorescence labeling, and excitation. It can achieve long-term three-dimensional observations of live specimens for weeks without cellular damage caused by photoactivation. The high resolution (under 150 nm lateral) achieved through synthetic numerical aperture provides sufficient spatial information to distinguish various subcellular compartments such as nuclei, nucleoli, mitochondria, and lipid droplets. Furthermore, analysis of the measured individual cell data can elucidate the temporal 3D volumetric dynamics with the dry mass information. This episode presents the latest development of...2024-04-081h 02
Listen In - Bitesize Bio Webinar AudiosUsing Antisense to Characterize Nonsense: A Mouse Model of Progranulin-Deficient DementiaTransgenic mouse models are important tools for testing therapeutic strategies. In this episode of Listen In, discover the benefits and essential considerations when characterizing and using transgenic mouse models to test therapeutic approaches to treat neurodegenerative diseases.Plus, explore a knock-in mouse model of frontotemporal dementia that harbors a common mutation in the progranulin gene to test an antisense oligonucleotide (ASO)-based therapeutic strategy.Watch the full presentation here: https://events.bitesizebio.com/using-antisense-to-characterize/joinBrowse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/2024-02-2642 min
Listen In - Bitesize Bio Webinar AudiosVisualizing Membrane Dynamics by Electron MicroscopyExplore how the latest electron microscopy techniques and volume imaging can capture fast, dynamic membrane and vesicle remodeling events during synaptic transmission at the ultrastructural level.Neurons communicate at specialized junctions, or synapses, via chemical messengers called neurotransmitters. Within a millisecond of neuronal stimulation, synaptic vesicles packed with neurotransmitters fuse with the presynaptic plasma membrane and release their contents. These neurotransmitters then bind and activate receptors on the postsynaptic membrane, resulting in cell-to-cell signal transmission. Then, to sustain this neurotransmission, new vesicles are recruited to the release sites. In this episode of Listen In, we...2024-02-1940 min
Listen In - Bitesize Bio Webinar AudiosBenefits of Imaging Data in Flow Cytometry Experiments: A Panel DiscussionExperience the transformative power of unbiased, data-driven cell analysis with access to over 25 image-derived label-free parameters, empowering you to easily assess sample quality, optimize gating strategies, and validate rare events.In this episode, learn how automated image analysis combined with flow cytometry can help improve data accuracy and precision, optimize and verify gating strategies, and discover novel biology, such as cell–cell interactions.Join a panel of experts as they discuss the features, benefits, and applications of a paradigm-shifting technology in flow cytometry with the Invitrogen™ Attune™ CytPix™ Flow Cytometer.Watch the full presenta...2024-02-1241 min
Listen In - Bitesize Bio Webinar AudiosEveryday Culture Practice: Improving Reproducibility in Cell CultureEukaryotic cell cultures respond to the slightest influence. Apart from the risk of contamination, minimal changes in cultivation parameters can affect their viability, growth, and cell metabolism. In this episode of Listen In, discover the measures you can take to improve the reproducibility of your experiments by incorporating Good Cell Culture Practice into your eukaryote culture routine.Explore the critical aspects of eukaryotic cell culture, including Mycoplasma test methods, effects of passage numbers, using Foetal Bovine Serum (FBS), and more!Watch the full presentation here: https://events.bitesizebio.com/everyday-culture-practice-improving-3/join...2024-01-0858 min
Listen In - Bitesize Bio Webinar AudiosPrecision and Efficiency at Your Fingertips: Why Electronic Pipettes are the Future of PipettingMany scientists see the added value in using electronic pipettes and have already upgraded their pipette fleet. The benefits include:• Reduced strain on thumbs and arms by improving pipetting ergonomics. • Increased reproducibility of experiments with motor-operated pipetting cycles. • Easy and efficient handling of high sample throughput.In this episode of Listen In, learn why switching to electronic pipettes can be beneficial for a variety of applications in the lab. Get answers to your typical questions regarding their usage, reliability, and ergonomics, and discover new options with connected electronic devices and the Eppendorf Pipette Manage...2023-12-1151 min
Listen In - Bitesize Bio Webinar AudiosIntroduction to Liquid Handling for Next-generation Sequencing WorkflowsNext-generation sequencing (NGS) has revolutionized the ‘omics’ era, with the rapid decoding of massive DNA sequences, supporting the investigation into various biological processes in human disease, genetic inheritance, immunity, cancer, and others.But generating high-quality NGS libraries is a labor-intensive process that requires experience, precision, and accuracy. Optimal, thorough sample preparation upstream of the sequencing process is essential to ensure the best possible results.In this episode of Listen In, get expert practical advice from Eppendorf on getting started with NGS in the lab, and discover how you can bypass pipetting-intensive protocols and automate your samp...2023-11-271h 04
Listen In - Bitesize Bio Webinar AudiosEssentials in Centrifugation—Better Safe than Sorry!Centrifugation is the cornerstone of lab life, used in nearly every workflow, be it a routine task like pelleting cells or a dedicated assay for an important biomolecular target.That's why it's such a pain when they break down.But just because we use centrifuges every day doesn't mean there aren't important technical aspects to consider or no parameters you can tailor to your specific application.In this episode of Listen In, learn everything you need to know before next using a centrifuge.Let the experts from Eppendorf guide you through...2023-11-131h 03
Listen In - Bitesize Bio Webinar AudiosHow to Keep Your ULT Freezer HealthyYour ultra-low temperature (ULT) freezer is not just a storage room for samples; it is your assurance for long-term scientific success and guards the results of your work. Therefore, every researcher should understand the importance of ULT freezers, know how to choose a reliable one, understand best practices for sample management, and have clear guidance on maintaining ULT freezers. In this episode of Listen In, learn the key considerations when buying a new ULT freezer for your lab and the best tips and tricks to prolong its lifetime. Plus, get advice on reducing the energy footprint of...2023-10-1651 min
Listen In - Bitesize Bio Webinar AudiosReal-time IntraVital Microscopy (IVM): In Vivo Cellular-level Imaging of Internal Organs in a Live AnimalIntravital imaging of cellular dynamics in a natural physiological microenvironment can provide unprecedented insights into the dynamic pathophysiology of diseases. In this episode of Listen In, get a demonstration of the latest technology to image inside the cells of live animals.Watch real-time multicolor sub-micron resolution image acquisition for dynamic cellular events, such as gene expression, cell trafficking, and cell–cell, and cell–microenvironment interactions in a range of organs. And explore the latest research that uses real-time intravital imaging to investigate dynamic cellular-level pathophysiology of various human diseases to develop novel therapeutics. 2023-09-111h 02
Cryo-TalkCryo-Talk interviews Rhys Grinter (Monash University)#12 — Rhys Grinter is Lab Head in the Department of Microbiology at Monash University. In this episode of Cryo-Talk, Rhys joins Eva Amsen to talk about how he uses cryoEM to look at bacterial proteins, including an enzyme that converts air to electricity. They also talk about travel, career breaks, and cooking.Watch or listen to all episodes of the Cryo-Talk podcast here: https://cryo-talk.bitesizebio.com2023-08-1623 min
Cryo-TalkCryo-Talk interviews Peter Shen (University of Utah)#11 — Peter Shen is an Assistant Professor of Biochemistry at the University of Utah. In this episode of Cryo-Talk, Peter joins Eva Amsen to talk about the CryoEM 101 course he co-developed and how this led to merit badges for the National Centers for CryoEM. They also talk about boardgames, basketball, and making music. Watch or listen to all episodes of the Cryo-Talk podcast here: https://cryo-talk.bitesizebio.com2023-07-1233 min
Listen In - Bitesize Bio Webinar AudiosBreaking the Ice: Freeze Fracture for Cryo SEMFreeze fracture is a powerful technique that enables imaging of internal structures of cells and tissues at the nanoscale level by freezing samples at ultra-low temperatures and then fracturing them along natural weak points. These structures can be imaged without dehydration or distortion using cryo-SEM, providing high-resolution images with great clarity.Discover how to use freeze fracture and cryo-SEM workflow to answer research questions and learn its exciting applications in cell biology.Watch the full presentation here: https://microscopyfocus.com/breaking-the-ice-freeze-fracture/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio...2023-07-101h 16
Cryo-TalkCryo-Talk interviews Gökhan Tolun (University of Wollongong)#10 — Gökhan Tolun is an Associate Professor in the School of Chemistry and Molecular Bioscience at the University of Wollongong in Australia, and Research Group Leader at the Molecular Horizons Research Institute. In this episode of Cryo-Talk, Gökhan joins Eva Amsen to talk about his research, funding challenges in different countries, and how Molecular Horizons' new facility was built with microscopy in mind. They also talk about photography, archery, and the two-body problem in science. Watch or listen to all episodes of the Cryo-Talk podcast here: cryo-talk.bitesizebio.com2023-06-1434 min
Cryo-TalkCryo-Talk interviews Bret Freudenthal (University of Kansas Medical Center)#9 — Bret Freudenthal is Associate Professor in the Department of Biochemistry at the University of Kansas Medical Center. In this episode of Cryo-Talk, Bret joins Eva Amsen to talk about the importance of making CryoEM technologies accessible via shared facilities and the new facility opening in Kansas. They also talk about skiing, barbecuing, and ‘90s rap music. Watch or listen to all episodes of the Cryo-Talk podcast here: cryo-talk.bitesizebio.com2023-05-1724 min
Listen In - Bitesize Bio Webinar AudiosThe Reproducibility Crisis: Why? How? What Now?Reproducibility is a cornerstone of the scientific technique, and something that is not reproducible is unlikely to be true.Yet despite its frequent neglect, reproducibility is fundamental. It enables scientists to continue and expand upon existing work and builds trust in science and the scientific method.So in what ways does the so-called reproducibility crisis hinder good research? What techniques and mindsets can we adopt to address it? How is it perceived by researchers in different areas of life science? And is poor reproducibility inevitable under current circumstances? Join Max Green, founder of...2023-05-1156 min
Cryo-TalkCryo-Talk interviews Ariane Briegel (Leiden University)#8 — Ariane Briegel is Professor of Ultrastructural Biology at Leiden University and co-director of the Netherlands Centre for Electron Nanoscopy. In this episode of Cryo-Talk, Ariane joins Eva Amsen to share how she uses cryo-electron tomography to study how microbes interact with their environment. They also talk about Ariane’s initial interest in marine biology and horseback riding. Watch or listen to all episodes of the Cryo-Talk podcast here: cryo-talk.bitesizebio.com2023-04-1220 min
Cryo-TalkCryo-Talk featuring Rob Kirchdoerfer (University of Wisconsin-Madison)#7 — Rob Kirchdoerfer is Assistant Professor in the Department of Biochemistry and the Institute for Molecular Virology at the University of Wisconsin-Madison. In this episode of CryoTalk, Rob joins Eva Amsen to talk about using cryoEM to study virus interactions and how he ended up working on cutting-edge research. He also talks about possible future cryoEM applications, why he has been interested in science since he was a kid, and winter in Wisconsin. Tune in to hear more!Watch or listen to all episodes of the Cryo-Talk podcast here: cryo-talk.bitesizebio.com2023-03-1518 min
Mentors at Your BenchsideHow to clean and calibrate your lab balance#41 — Keeping your lab balance clean and calibrated will make your bench work a lot easier. In this episode we give you useful tips to maintain your balance, literally! Visit our website to read the full article. [1]Read more about accuracy and precision [2] and the types of statistical error [3] you can avoid by calibrating your lab balance. For more information, see this guide on cleaning and maintaining balances. [4]Resources:1. How to Clean and Calibrate Your Lab Balance. Available at https://bitesizebio.com/24193/how-to-clean-and-calibrate-your-lab-balance/2. How to Measure and Improve Lab Accuracy and Precision. Av...2022-12-1310 min
Mentors at Your BenchsideHow to Write a Thoughtful Discussion for Your Scientific Paper#40 — Are you struggling to write the discussion section of your paper? It can be daunting to put your results in context and to thoughtfully consider limitations and future experiments, but we've collected some useful advice to help you on your way. Visit our website to read the full article. [1]If you need more help with your article, check out some of our other resources, such as this guide on writing an abstract, [2] preparing figures for your paper [3] and choosing a journal to submit to. [4] And if you want to know more about the publication process in...2022-12-0610 min
Cryo-TalkCryo-Talk featuring Mimi Ho (Columbia University)#6 — Mimi Ho is Assistant Professor of Microbiology and Immunology at Columbia University. In this episode of Cryo-Talk, Mimi joins Eva Amsen to talk about her career journey from industry to academia, her support network, and how Mighty the dog has been helping in the lab. She also shares what it has been like to co-host The Plunge podcast. Tune in now to hear more!2022-11-1630 min
Mentors at Your BenchsideHow to Check the Accuracy of Your Pipette in 7 Easy Steps#36 — Don't let an inaccurate pipette ruin your experiments. In this episode of Mentors At Your Benchside we’re talking about how to check the accuracy of your pipette, so your results are reliable and repeatable. You can find the full article on our website. [1] Read more about cleaning [2] or calibrating pipettes [3] or learn how clean and calibrate your lab balance. [4] For more information on accuracy and how it differs from precision, see our article "How to Measure and Improve Lab Accuracy and Precision". [5]Resources:1. How to Check the Accuracy of Your Pipette in 7...2022-11-0807 min
Mentors at Your BenchsideHow to Make the Perfect Agar Plate Every Time#35 — A smooth, perfect agar plate will make your microbial experiments a lot easier. Whether you're using LB, M9 or blood agar, tune in to this episode to learn how to pour the perfect plates every time.Visit our website to read the full article. [1]Explore other practical secrets of the bacterial world [2] on our website or find out how to avoid lab disasters. [3] Resources:1. How to Make the Perfect Agar Plate Every Time. Available at: https://bitesizebio.com/6938/how-to-make-the-perfect-agar-plate-every-time/2. Practical Secrets of the Bacterial World for the Uninitiated. Available at: https://bite...2022-11-0106 min
Cryo-TalkCryo-Talk interviews Mike Cianfrocco (University of Michigan)#5 — Mike Cianfrocco is Research Assistant Professor at the University of Michigan Life Sciences Institute and Assistant Professor in the Department of Biological Chemistry at the University of Michigan Medical School. In this episode of Cryo-Talk, Mike joins Eva Amsen to talk about the tools he is developing for cryoEM users, such as COSMIC2. He also chats about his love of gardening and fermented foods. Tune in now to hear more!2022-10-1920 min
Mentors at Your BenchsideIs Your Bacterial Culture Still Growing? A Primer on OD600 Measurements#32 — How do you know if your bacteria culture is ready for your experiment? In this episode of Mentors At Your Benchside we take a close look at the popular method of OD600 measurements as well as some other techniques you can use to find out if your bacterial culture is still growing.You can find the full article on our website. [1]Learn more about different bacterial strains [2] and get to grips with growth curves [3], or find out how to train yourself to measure OD600 by eye [4]. And if you're curious about sporulating bacteria, read this ar...2022-10-1113 min
Mentors at Your BenchsideProtease Inhibitors 101: How They Work and How to Use Them#29 — Ever wondered why you have to add protease inhibitors to your lysis buffer? In this episode of Mentors At Your Benchside we’ll tell you all about protease inhibitors, why they’re important and how to use and store them.To learn more about protease inhibitors and to access the table with information about common protease inhibitors, read the full article on our website [1].Read more about how protease inhibitors work [2], learn about protein expression [3] and find out what you need to consider during cell lysis [4].Resources:1. Protease Inhibitors 101: How They Work a...2022-09-2709 min
Cryo-TalkCryo-Talk interviews Liz Kellogg (Cornell University)#4 — Liz Kellogg is Assistant Professor in the Department of Molecular Biology and Genetics at Cornell University. In this episode of Cryo-Talk, Liz joins Eva Amsen to share how she uses cryoEM to learn more about CRISPR-associated transposons. We also hear about the challenges of keeping a new lab going during the early days of COVID and find out what her favorite music is. Tune in to hear more!2022-09-2119 min
Cryo-TalkCryo-Talk interviews Yiorgo Skiniotis (Stanford University)#3 — Yiorgo Skiniotis of Stanford University has been using cryoEM to study transmembrane receptors. In this episode of Cryo-Talk, Yiorgo joins Eva Amsen to chat about the potential of cryoEM to gather more information about signaling pathways. We also hear more about his love of cinema and classic literature, why he’d be a fisherman if he had to pick another job, and why it’s so important to have various research interests. Tune in to hear more!2022-08-2429 min
Mentors at Your BenchsideHow (and Why) To Manipulate Plasmid Copy Number#17 — Why do some plasmids give a better protein yield than others? It may have a lot to do with the plasmid copy number. In this episode we talk about what plasmid copy number means, why it is important and how you can manipulate it to get the most out of your experiments. To learn more about plasmid copy number, read the full article on our site: https://bitesizebio.com/22824/how-to-manipulate-plasmid-copy-number/2022-08-1609 min
Mentors at Your BenchsideA Brief History of CRISPR-Cas9 Genome-Editing Tools#15 — In the last few years, CRISPR-Cas9 has become a popular genome editing tool, but have you ever wondered where it came from? In this episode we explore the history of CRISPR-Cas9, from the first CRISPR discovery in 1987 to current applications in targeted genome and epigenome editing.Read the full article to learn more about the history of CRISPR-Cas9 and to see a timeline of discoveries here - https://bitesizebio.com/47927/history-crispr/2022-08-0908 min
Cryo-TalkCryo-Talk interviews Eva Nogales (UC Berkeley)#2 — Eva Nogales of UC Berkeley and Lawrence Berkeley National Laboratory uses cryoEM to study cellular processes related to cytoskeletal self-assembly and gene expression. In this episode of Cryo-Talk, Eva joins our host Eva Amsen to discuss the use of CryoEM to study complex cell biology systems and more. She chats about her current work while on sabbatical at CNIO in Spain, what music she likes, and her love of books. We also hear why she thinks it’s so important to work with people that you get along with. Tune in to hear more!2022-07-2029 min
Mentors at Your BenchsideWhat the HEK? A Beginner’s Guide to HEK293 Cells#9 — One of the most commonly used cell lines in molecular biology labs is the Human Embryonic Kidney HEK293 cell line. Listen to this episode to find out all about the history of HEK293 cells and how to work with them. Read the full article [1] for more details about working with HEK293 cells Resources:1 - https://bitesizebio.com/45489/guide-to-hek293-cells/2022-07-1907 min
Mentors at Your BenchsideWhat is PCR? The Beginner’s Guide#4 — PCR is a useful lab technique used to copy, sequence or quantify DNA. If you're new to PCR, this episode is for you. We cover the five things you need to get started with PCR and explain how to run a polymerase chain reaction. Read the full article to learn more about setting up a PCR. [1]Learn more about Kary Mullis and the invention of PCR, and about the first thermocycler "Mr Cycle". [2, 3, 4] For practical tips, see our article on the top tips for primer design. [5]Resources:1. What is PCR? The Beginner’s Gu...2022-07-0111 min
Cryo-TalkCryo-Talk interviews Joachim Frank (Columbia University)#1 — Joachim Frank of Columbia University has spent his career working on EM and cryoEM. In this episode of Cryo-Talk, Joachim joins Eva Amsen to discuss his research and his 2017 Nobel Prize in Chemistry. We’ll hear how he has used peripheral vision to find unexpected opportunities, why he loves fiction writing, and how he balances New York City life with his time in the Berkshires. We also learn about his Master’s project studying the back-scattering of electrons on liquid gold, his first post-doc at the Jet Propulsion Laboratory, and the conversation turns to butterflies on multiple occasions. Tune in to...2022-04-1336 min
GradBloggerSciComm for Researchers with Dr. Eva Amsen | GBP051In this episode of the GradBlogger podcast, Dr. Eva Amsen joins us for part two of our discussion about science communication and her newest workbook.2020-03-2600 minGradBloggerBuilding a Career in Science Communication with Dr. Eva Amsen | GBP050In this episode of the GradBlogger podcast, Dr. Eva Amsen joins Dr. Chris Cloney to talk about building a career in science communication.2020-03-1900 min
Recovering AcademicSeason 4, Episode 1: Eva AmsenEva Amsen, PhD. Photo from Easternblot.net. Photographer: Rannie Turingan
For our series four opener, we talk with Freelance writer, editor and science communicator Eva Amsen, PhD who joined us in the middle of her night from London. Eva got her PhD at the University of Toronto where she started a blog, EasternBlot.net. During her PhD, Eva had already decided that she didn't want to pursue a faculty career.
Through her blogging experience, she freelanced through the economic crash of 2008 just after her PhD. Giving herself a one year deadline post PhD to find a job, she landed a...2019-11-0109 min
The Opposite Of LonelyNew in Town.
“I kind of grew up on the internet, with a lot of friends in different
places all over the world. I don’t see a distinction between internet
friendships and ‘real’ friendships. So when I moved, most of my friendships
didn’t change at all, because they were already based on internet
communication or on phone calls.”
You’re moving to a new city! It’s an exciting chance to start fresh, maybe re-invent yourself a bit with a whole new social circle. But are you going to
lose the friends you made in the last place you lived? And h...2018-07-2551 min
The Opposite Of Lonely7. New in Town.You’re moving to a new city! It’s an exciting chance to start fresh, maybe re-invent yourself a bit with a whole new social circle. But are you going to lose the friends you made in the last place you lived? And how will you start meeting new people? Eva Amsen and Daniel Price join Nadia to discuss.2018-07-2400 min