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ひらよしのらじお（仮）
ひらよしのらじお（仮）2025/09/16：【016】ひらいずみさんの、らじお。 from Radiotalk引用URL 古紙再生促進センター http://www.prpc.or.jp 紙リサイクルの基礎知識 http://www.prpc.or.jp/recycle/waste_paper 蛇足：最近では使用済みの昇華転写紙をRPFという固定燃料（石炭の代替燃料）への再資源化が行われているようです。 石坂産業株式会社様による使用済み昇華転写紙の回収 https://youtu.be/fBd3XT5mkZ8?si=y-GK6XcEk6C3eSMF #収録の日2509 #AMひらお #ひらいずみさんの、らじお。 #昇華転写紙 ⭐️⭐️⭐️⭐️ ⭐️⭐️⭐️⭐️ #ひらよしのらじお（収録） #ぴんく放送局120 ぴんく放送局の公式ホームページURL https://voicefan.net/voic-fan-radio/pink2024-pinku-broadcasting-station ⭐️⭐️⭐️⭐️ ⭐️⭐️⭐️⭐️
2025-09-1612 minWomen In Industry Podcast
Women In Industry PodcastFrom Stigma to Solutions: Pelvic Health with Sandy and SarahIn recognition of Pelvic Floor Dysfunction Awareness Month, this episode features two leading experts from Advanced Physical Therapy who are helping educate listeners on a topic that affects millions but is rarely discussed.Sandy, Director of Clinical Education and Pelvic Health Program Director, has been with Advanced Physical Therapy since 2011. She specializes in pelvic health physical therapy, treating men, women, and pediatric patients. She holds the Pelvic Rehabilitation Practitioner Certification (PRPC) and is certified in pessary fitting and management—offering specialized care for a wide range of pelvic floor conditions.Sarah Walker, PhD, PT, DPT, PR...
2025-08-0627 minPelvic Service Announcement
Pelvic Service AnnouncementHW Connect 2025Rachel attended the HW Connect Conference, and she can't wait to tell you all about it! In this episode, she shares insights from several speakers, including Dawn Sandalcidi, PT, RCMT, BCB-PMD; Nancy Norton, RN; Leticia Nieto, Psy.D., LMFT; Holly Tanner, PT, DPT, MA, OCS, WCS, PRPC, LMP, BCB-PMD, CCI; Ken McGee, PT, DPT (they/he); Dr. Cindy Mosbrucker; Carole High Gross, PT, DPT, PRPC; Kathleen D. Gibson, MD; and Ramona C. Horton, MPT, DPT.
2025-05-1353 minThe Menopause Health Podcast
The Menopause Health PodcastPelvic Health, Lymphedema, and Hormone Therapy: Transforming Menopause Health with Sheree Dibiase, PT, PRPC, ICLM, DNJoin Susan Sly for a powerful episode of the Menopause Health Podcast featuring Sheree DiBiase, Founder and President of Lake City Physical Therapy, PA, a premier provider of personalized physical therapy services in the Northwest. With over 40 years of leadership in women’s health, oncology, sports, and orthopedics, Sheree reflects on her personal menopause journey and shares invaluable insights into the lymphatic system, pelvic health, and hormone therapy. Drawing on her expertise as a certified Pelvic Rehabilitation Practitioner (PRPC) and International Lymphedema Management specialist (ICLM), she highlights key symptoms like heaviness, bloating, and pelvic pain. Learn practical str...
2025-01-1542 minThe Lamaze Podcast
The Lamaze PodcastWhat Does Pelvic Floor Therapy Have to Offer?In this episode of The Lamaze Podcast, host Joy Skow, CD, LCCE, talks with Dr. Amanda Heritage, PT, DPT, PRPC; and Dr. Becca Jones, PT, DPT, CSCS, about the vital role pelvic floor therapy plays during pregnancy, labor, and postpartum recovery. They highlight how fostering curiosity about the pelvic floor can build confidence and discuss the benefits of practicing pelvic floor therapy to prepare the body for all stages of life. Additionally, they explore how following the body’s natural urges to push — one of the Lamaze Six Healthy Birth Practices — aligns with pelvic floor therapy techniques.  Dr. Ama...
2024-10-0958 minLove Mia Vita
Love Mia VitaOveractive Bladder and Pelvic Floor PT w/ Dr. Alexandra Bertucci, PT, DPT, PRPCHow Pelvic Floor Physical Therapy can help relieve symptoms of overactive bladder,stress urinary incontinence (SUI), and mixed urinary incontinence (MUI). Gerie DiPiano's special guest this episode is Dr. Alexandra Bertucci, PT, DPT, PRPC.
2024-09-1034 minPelvic Service Announcement
Pelvic Service AnnouncementStay Curious, Stay Open (Feat. Holly Tanner, PT, DPT, MA, OCS, WCS, PRPC, LMP, BCB-PMD, CCI)Get ready for one of the most exciting guests ever to be featured on Pelvic Service Announcement. This week, the PSA girls were honored to host Holly Tanner, PT, DPT, MA, OCS, WCS, PRPC, LMP, BCB-PMD, CCI for the first-ever Herman & Wallace podcast collaboration. Holly is the Director of Education for the Herman & Wallace Pelvic Rehabilitation Institute and shared some valuable insights into the world of pelvic health education, managing complicated cases, and navigating interpersonal relationships in the clinic. Whether you're a patient, clinician, or just someone with a love for pelvic health, this is an episode you don't want...
2024-05-071h 05One Simple Step Today
One Simple Step TodayPelvic Health 101This is the re-aired episode of Java with Juli podcast, an outreach of Authentic Intimacy (www.authenticintimacy.com). Dr. Juli Slattery and I discussed everything Pelvic Health. No topic was off-limits. Listen and learn! If you have any questions, you can reach me at: tech@oneismplestep.today
2024-04-2345 minOTs In Pelvic Health
OTs In Pelvic HealthParkinsons + Pelvic HealthOT Pioneers: Intro to Pelvic Floor Therapy  opens January 15-19, 2024We'd love to welcome you to Pelvic OTPs United - Lindsey's off social media community ($39 a month with no obligation!)Meet my guest: Erica Vitek, MOT, OTR, BCB-PMD, PRPCErica has been an occupational therapist at Aurora Health Care at Aurora Sinai Medical Center in downtown Milwaukee, Wisconsin for over 20 years. Erica is Board Certified in Biofeedback for Pelvic Muscle Dysfunction (BCB-PMD), Board Certified Pelvic Rehabilitation Practitioner (PRPC) through Herman and Wallace Pelvic Rehabilitation Institute, LSVT BIG and PW...
2023-12-1857 minThe Be More Today Show
The Be More Today ShowBMT EP 133: “There’s MORE to your Pelvic Floor” featuring Michelle Dela Rosa PT, DPT, PRPCOur celebration of National Physical Therapy Month continues with Michelle Dela Rosa. Join our conversation as we debunk the taboos surrounding pelvic physical therapy, discuss how pelvic pain affects both men and women, and prove why there is hope for anyone to have a healthier pelvis. Michelle Dela Rosa, PT is the Clinical Director of the Hamilton and Howell locations at Jag Physical Therapy. She has been practicing in the field of pelvic health physical therapy for 20 years. She started in Brooklyn, NY and developed the pelvic PT program, in now NYU Langone Health. She implemented a weekly prenatal exercise...
2023-10-0959 minBetween Two Lips
Between Two LipsWeights, Wands and Other Vagina Self Care ToolsDr Amanda Olson is my guest today.  Here is her bio and more details on where to find her and her amazing products.I am a dedicated and passionate physical therapist specializing in treatment of pelvic floor disorders including incontinence, pelvic organ prolapse, pelvic pain, and pregnancy related issues. I earned my doctorate degree in 2008, and I have years of diverse clinical experience. Additionally, I am certified as an advanced pelvic floor practitioner (CAPP-PF) by the American Physical Therapy Association and the Herman and Wallace Pelvic Institute (PRPC).I am proud to be the President a...
2023-02-1548 minBetween Two Lips
Between Two LipsPelvic Organ Prolapse - Is This The Miracle Cure?Becky has been a Physical Therapist for over 22 years, and has been specializing in Pelvic Health Physical Therapy since 2009. She earned her Pelvic Rehab Practitioner Certification (PRPC) in 2016. Her current role is regional VP and clinician for Activcore Physical Therapy and Performance in Princeton NJ. She has become passionate about hypopressives since she was first  introduced to Low Pressure Fitness in 2016 and continued on with her training to become Low Pressure Fitness (LPF-CERT) certified. Since 2020, she has been instructing the LPF professional certification courses in English, the distributor of LPF USA and earned the distinction of LPF m...
2023-02-081h 18About IBD
About IBDPelvic Health With Amanda Olson, DPT, PRPCCaring for pelvic health is important for people of all genders who live with an inflammatory bowel disease (Crohn's disease or ulcerative colitis). Dr. Amanda Olson, who holds a doctorate in physical therapy and is the President and Chief Clinical Officer for Intimate Rose, has dedicated her professional life to helping people improve their pelvic health. Learn more about pelvic therapy and how she has developed resources and tools to help people living with all types of conditions. Find Amanda Olson, DPT, PRPC at: Instagram: @intimaterose Twitter: @IntimateRose Web: Intimate Rose Buy: Restoring The Pelvic Floor...
2023-01-2428 minBeyond The Mat
Beyond The MatLearning the basics of pelvic floor PT, with Ronit Sukenick, PT, DPT, PRPCWelcome to Season 2! In this episode of Beyond the Mat, we are joined by Ronit Sukenick, a pelvic floor physical therapist. We go over all the basics, what is pelvic floor therapy, who would benefit, and what kinds of issues can be treated. Spoiler alert: Leaking is common, but not normal and can easily improve! For more info on Ronit, please visit https://rspelvicpt.com/.
2023-01-1037 minThe Language of Creativity Podcast
The Language of Creativity PodcastA Literal Pain in the Ass - Elizabeth Makous (Pelvic-Floor Physical Therapist) Ep.26“You have pain where?!” People have often asked why a healthy person like me would need physical therapy. They are quite surprised to learn about pelvic-floor therapy… and it’s not long before I hear “I have pain there too.” What was once a specialty that not even many obstetricians or urologists knew about, Pelvic Floor Physical Therapy is now a rapidly growing field with an outsized demand for new therapists. Treating conditions like urinary and fecal urgency, incontinence, prolapse, nerve entrapment, surgical scarring, pre and postpartum issues, visceral restrictions and sexual dysfunction, it’s not hard to see why.
2022-09-271h 18Pain Science Education
Pain Science EducationEpisode 284 | Development Of A Community Based Comprehensive Childbirth, Postpartum, And Women's Health Empowerment Model With Theresa Feola, PT, WCS, PRPCPelvic health may not be the first thing that comes to mind when you think of women's health, yet it is an important issue that deserves attention. In today's episode, Theresa Feola , PT, WCS, PRPC, of Pelvic Room joins Dr. Joe Tatta. Theresa is a board-certified women's health therapist. She has served in clinical management roles throughout her career, but her clinical focus has been on the education and treatment of women's and men's pelvic health. Today, Theresa discusses the current state of women's health, the challenges women face in accessing appropriate pelvic health and physical therapy, and her...
2022-08-1041 minPelvic Floor At Its Core
Pelvic Floor At Its CoreDr. Amanda Olson - Creator of Intimate Rose Products: Vaginal Weights, Dilators, and MoreBreak the stigma about keeping pelvic health talk behind closed doors. Join us, Flyte Therapy @flytetherapy, in chatting with pelvic health experts about all things pelvic floor and beyond. Who are we? We are a product (known as Flyte, we strengthen the pelvic floor when used for 5 min daily x 6 weeks, and rid of bladder leaks) and service company focused on meeting women where they are, empowering and supporting women in their pelvic health journeys, and increasing access to pelvic health. Check us out at www.flytetherapy.com  Now let's get to the Core of t...
2022-08-0531 minNutrition Rewired
Nutrition RewiredEp. 49 - Pelvic floor health, digestion, and sexual function with Dr. DiGrado PT, DPT, PRPCIn today's episode we discuss: - How pelvic floor impacts digestion and vice versa - How pelvic floor therapy can improve sexual function - The impact of birth control on female sexual health To work with Dr. DiGrado: https://bostonpelvicpt.com/meet-your-pts To work with Erin: Nutritionrewired.com
2022-08-0449 minThe Lamaze Podcast
The Lamaze PodcastPelvic HealthOn this episode of The Lamaze Podcast, Priyanka Banerjee, BSc, MSc, a Content Strategist and Infant Development Consultant with DC Birth Doulas, speaks with Gopi Pillai, PT, DPT, PRPC about the benefits of pelvic floor physical therapy. Gopi is the owner of Blueprint Physical Therapy and specializes in Pelvic Health.
2022-07-0631 minHomeschool Conversations with Humility and Doxology
Homeschool Conversations with Humility and DoxologyPelvic Health: pelvic prolapse, diastasis recti, and other common pelvic floor challenges (with Dr. Meghan O'Hara)⚠ In today’s episode we’re going to be talking about issues surrounding female health, particular pelvic floor dysfunction. If you usually listen to the podcast episode with little ones listening in, I just wanted to give a heads up that we will be discussing anatomy and having a frank conversation. This is probably one to listen to with earbuds. ⚠ It’s a bit of a cultural joke that moms can’t jump or laugh without a little bladder leakage. But it’s actually no laughing matter. Not only that, but the symptoms associated with a weak pelvic floor c...
2022-06-211h 08OTs In Pelvic Health
OTs In Pelvic HealthCan OTs and PTs Collaborate in Pelvic Health?Get my 9 page OTs Map to the World of Pelvic Health.Meet me on the OTs for Pelvic Health Facebook Group!Check Out More OT Pelvic Health Content here.Find me on IG! @functionalpelvis-- Transcript -- No matter if you're in OT or PT, so it doesn't matter whether there's an O or a P in your credentials - what's most important is if there's a T. We are all therapists at the end of the day.   All of us hav...
2022-06-1516 minBowel Moments
Bowel MomentsMeet Dr. Smita M.- Pelvic Floor Physical Therapist! - Episode 18Send us a textThis week we talk to Dr. Smita Mehta, PT, DPT, PRPC! Dr. Mehta is a pelvic floor physical therapist and part of a multidisciplinary team for IBD at Brooke Army Medical Center in San Antonio, TX. She clarified what pelvic floor PT is, how it can help people living with IBD, how to find your pelvic floor, and so much more. This conversation was absolutely fascinating and a revelation for both of us. And Dr. Mehta is so passionate about her work! In addition to her impressive resume on pelvic floor rehabilitation, she...
2022-05-2556 minSluts and Scholars
Sluts and Scholars210 Sex with Dr. JessDr. Jess O'Reilly is an acclaimed international sex educator, author, and podcaster. This episode we talk about core erotic feelings, sexual values, "laziness" in relationships, and even why you shouldn't JIC (just in case) pee!   Dr. Jess: https://www.sexwithdrjess.com/   Book mentioned: https://www.amazon.com/Laziness-Exist-Devon-Price-Ph-D/dp/1982140100   Past episode on pelvic floor therapy: https://slutsscholars.libsyn.com/148-the-pelvic-whisperer-with-dr-sherine-aubert-pt-dpt-prpc   FOLLOW US  Twitter Instagram Facebook Send questions, comments, stories, rants to: SlutsAndScholars@gmail.com Sluts And Scholars is a production of sluts and scholars media.
2022-05-1950 minThe Pelvic Floor Project
The Pelvic Floor Project48. Using dilators to address painful penetration with Dr. Amanda OlsonIn this episode, I sit down with Dr. Amanda Olson, physical therapist and pelvic expert behind Intimate Rose to discuss:The messaging that dilators are used to "stretch tight tissue" Reasons why penetration with intimacy, tampon use and medical exams can be painfulThe connection between brain and pelvic floor How dilators can be used to help the brain and body tolerate penetrationIntimate Rose product details (sizing, what they are made of, lubrication to accompany, ordering)Ways to progress when using a dilator How to know if a dilator(s) would be an option for youDr. Olson ear...
2022-05-0944 minHello Postpartum
Hello PostpartumWhen It's Time To Ask for Pelvic Floor SupportListen in on Dr. DiGrado (Alex) as she explains the difference between a pelvic floor that is too loose or too tight and common pelvic floor issues in postpartum – you might be surprised. 020 | Alexandra DiGrado of Boston Pelvic Floor Therapy What is the pelvic floor, and why is it important to postpartum mothers? This is a question many new mothers should be asking. Unfortunately, there isn’t much information about the importance of pelvic floor health for those who have just given birth. In this episode, I chat with pelvic floor ther...
2022-04-1738 minThe PelviBiz Podcast
The PelviBiz PodcastTools For Improving Pelvic Floor Strength with Amanda OlsonCan you imagine helping your client live life without their pelvic issues? Could you imagine the feeling of freedom and wellness that would give them from simply being pain-free every day? Welcome to the PelviBiz Podcast! I’m your host, Dr. Kelly Alhooie. In this week’s episode, I will be joined by Amanda Olson. Amanda found herself enduring a personal and tragic accident while on a camping trip with her husband and friends. She sustained a dislocation to her coccyx and significant damage to the muscles, tendons, and ligaments of her pelvic floor after a 40-foot fall...
2022-03-1926 minWomen\'s Health Care with Dr. Bruce Pierce
Women's Health Care with Dr. Bruce PiercePelvic Health Physical TherapyListen to my discussion about pelvic floor (health) physical therapy with Michelle Dela Rosa PT, DPT, PRPC. physical therapist.
2022-03-1737 minTherap-ish
Therap-ishIs it normal to pee a little when you sneeze or laugh??Hanging out with Daphna Ross and discussing whether or not its OK to experience all kinds of urinary issues as women.   Daphna Ross, PT, PRPC, WCS\Board Certified Specialist Pelvic and Women's Health Physical Therapy  email: daphna@womenshealthhealingpt.com    Phone: 510-255-3865 website: https://www.womenshealthhealingpt.com/ This podcast is for educational and entertainment purposes and the information in it does not constitute or is not intended as therapy.  If you are experiencing a clinical issue that was discussed on the show please talk to your doctor for a therapist referral.
2022-03-0824 minSheConfidential® Podcast
SheConfidential® PodcastPelvic Floor Dysfunction & Physical Therapy feat. Dr. Tieya M. QuallsOur bodies are wonderful machines capable of creating new life, but many of the things we're taught about our bodies are flat out wrong. If you've ever suffered from painful intercourse,  constipation, chronic yeast infections or "leaks", you may want to consult someone like Dr. Tieya M. Qualls, a physical therapist with a specialty in pelvic issues.This conversation is also for those who may not be experiencing any discomfort but want to be proactive in managing their pelvic health. Pelvic health is related to the function of our bowel, bladder, and reproductive organs.In t...
2021-09-1650 minBarathi Thambi
Barathi Thambiஅனைத்து சாதியினரும் அர்ச்சகர்... வழக்கு, வரலாறு, வருங்கால சவால்கள்!கருவறை தீண்டாமையை அகற்ற வேண்டும் என்பது தமிழ்நாட்டின் 50 ஆண்டு கால நெடுங்கனவு. இதற்கு பெரியார் முன்மொழிந்த வழிமுறைகளில் ஒன்று அனைத்து சாதியினரையும் அர்ச்சகர் ஆக்குவது. முதல்வராக இருந்த கருணாநிதி இதை ஒரு சட்டத்தின் வழியாக செயல்படுத்த முனைந்தபோது, பல்வேறு வழிகளில் தடைகளை ஏற்படுத்தியது பார்ப்பனர் தரப்பு. ஏகப்பட்ட வழக்குகள், மேல் முறையீடுகள். அனைத்தையும் கடந்து தற்போது மு.க.ஸ்டாலின் தலைமையிலான அரசு, முதல் கட்டமாக 28 அர்ச்சகர்களை மாநிலம் முழுவதும் உள்ள கோயில்களில் பணிநியமனம் செய்துள்ளது. இவர்கள் அனைத்து சாதிகளையும் சேர்ந்தவர்கள். உண்மையில் இது சமூக நீதி அரசியலில் ஒரு பாய்ச்சல். சாதி பாகுபாட்டை உடைத்து தகர்க்கும் உள்ளடக்கம் கொண்டது.   இந்த அரசியல் முக்கியத்துவம் வாய்ந்த நடவடிக்கையின் பல்வேறு கூறுகள் பற்றி விவாதிக்கிறது இந்த உரையாடல். Twitter space-ல் நடைபெற்ற இந்த உரையாடலில் பங்குபெற்றோர்... 01. ஸ்ருதிசாகர் யாமுனன், பத்திரிகையாளர்;  02. தோழர் மருதையன்; 03. வழக்குரைஞர் வாஞ்சிநாதன், PRPC மாநில ஒருங்கிணைப்பாளர்; 04.ரெங்கநாதன், தலைவர், அர்ச்சகர் பயிற்சி பெற்ற மாணவர்கள் சங்கம். 
2021-08-201h 43Bob\'s Short Story Hour
Bob's Short Story HourEpisode 97 - Decameron Project Part 1Episode 97 includes the first part of a ten-part series in which we embark on a journey through the 100 stories told within Giovanni Boccaccio's book "The Decameron". This series, just as the book, will be divided into the ten days of the frame story. Selected stories from each day will be read by various artists from multiple sources. Series dedicated to Daniele Bolelli after his initial suggestion of the book back in Episode 90 of the show.  Story Timing Index:  Stories of Day 1: - Story 5 (Read by Abby Brenker) - 10:29 - Story 7 (Read by Jon Co...
2021-07-1141 minBarathi Thambi
Barathi ThambiFr. ஸ்டேன் சாமி மரணம் - மோடி அரசின் கொட்டடி படுகொலை!பீமா கோரேகான் வழக்கில் பொய்யாக சேர்க்கப்பட்டு, ஓராண்டுக்கும் மேல்  UAPA சட்டத்தில் சிறையில் அடைக்கப்பட்டு, கடைசியில் 84 வயதில் ஒரு விசாரணை கைதியாக மும்பையில் இறந்து போனார் அருட்தந்தை ஸ்டான் சுவாமி. திருச்சி - பூதலூர் அருகே உள்ள ஒரு சிறிய கிராமத்தில் பிறந்து, ஜார்கண்ட் மாநில ஆதிவாசிகளின் நலன்களுக்காக 50 ஆண்டுகளை செலவிட்ட ஸ்டேன் சாமியின் தியாகத்தையும், அர்ப்பணிப்பையும் சிறுமைப்படுத்தி, அவரை ஒரு பயங்கரவாதியாக சித்தரித்தது இந்த அரசு. அவர் மரணம் அடைந்த, 2021, ஜூலை 5-ம் தேதி Twitter space-ல் நடத்தப்பட்ட கண்டனக் கூட்டத்தின் இணைப்பு இது. இதில் கலந்துகொண்டோர், 01. பாலன், வழக்குரைஞர், பெங்களூரு, 02.வாஞ்சிநாதன், மாநில ஒருங்கிணைப்பாளர், PRPC, 03.மருதையன், 04.நாதன், 05. அசீப், பத்திரிகையாளர், 06.விக்ரமன், செய்தித் தொடர்பாளர், விடுதலை சிறுத்தைகள் கட்சி.  
2021-07-101h 31Murder Squared
Murder SquaredThe Murder Of Jay OrbinJay Orbin is missing for 12 days without anyone noticing. When police look into the victims life quickly a long list of suspects is formed. To support murder squared always rate, review, and subscribe and check out our link tree! https://linktr.ee/Murdersquaredpodcast Sources: Scorned Love Kills: S1E2 Betrayed: Life and Limb State v. Orbin, No, 1 CA-CR 14-0196 PRPC --- Support this podcast: https://anchor.fm/murdersquredpodcast/support Learn more about your ad choices. Visit megaphone.fm/adchoices
2021-07-0920 minReal Talk with the Pelvic Docs
Real Talk with the Pelvic DocsReturn To Running After Baby With Dr. Amanda OlsonIn today’s episode, we get real on running after having a baby. We first discuss common mistakes women make when starting to run again then we tackle how she helps novice and advanced runners run again. Postpartum Tissue Healing Shopping List referenced in the episode:Vulvar Balm to restore moisture while in a more low estrogen state (Like a chapstick for your vulva)!Personal Lubricant which is a must for sex! Pelvic Wand for gentle mobilization of the vaginal tissue to prep for intercourse!Kegel Weights to help buil...
2021-06-2755 minDr. Bri Talks Lady Bits
Dr. Bri Talks Lady BitsEp 67: Creative Care for Pelvic Pain w/ The Pelvic Whisperer (Therawand and Electrical Stimulation)May is Pelvic Pain Awareness month, and I'm continuing our pain series with Elizabath Makous, AKA "The Pelvic Whisperer." Even if you don't have pain, if you appreciate creativity, bio-individuality, and getting to the ROOT of an issue, then you're going to love this interview. Elizabeth shares: How to find a great physical therapist  The importance of differential diagnosis (in PT) and of creating individualized treatment plans How Elizabeth helped design and refine Therawand tools for pelvic pain relief at-home The importance of getting to know your body and how to listen to your tissues Some very...
2021-05-1233 minPelvic Matters
Pelvic MattersVaginal Weights, Dilators, and Wands with Dr. Amanda Olson DPT, PRPCOn this episode, Marina is joined by fellow pelvic health physical therapist Dr. Amanda Olson DPT, PRPC to discuss the use of vaginal weights, wands and dilators with patients who have pelvic floor dysfunction. Dr. Olson is the president and CCO of Intimate Rose where she develops pelvic health products and education. She's author of the book Restoring the Pelvic Floor For Women.  Listen to find out -What inspired Dr. Olson to become a pelvic floor physical therapist -What is the difference between vaginal weights, wands, and dilators, and who benefits from them -What is the best practice for a...
2021-04-3028 minAt Home With Ashley G
At Home With Ashley GNatural Birth, Doulas and Prenatal/Postnatal YogaJoin me as I discuss natural birth, doula support and prenatal/postnatal Yoga!  For Your Reading: Cribsheets by Emily OsterExpecting Better by Emily OsterThe Birth Partner by Penny SimkinSpiritual Midwifery by Ina May GaskinTaking Charge of Your Fertility by Toni WeschlerYoga Mama, Yoga Baby by Margo Shapiro BachmanYoga Mama by Linda SparroweIna May’s Guide to Childbirth by Ina May GaskinArticles Factors That Influ...
2021-04-2828 minSex Care is Self Care
Sex Care is Self CareSHE+ #2 Libido & DesireMany women will experience a lowered libido at some point in their lives, the reality is that there is no such thing as a “normal” sex drive. This can lead to a lot of confusion for women wondering if this is just a phase they are going through or if the issue is a persistent concern that needs to be addressed. To help clear up the confusion, on today’s episode of Sex-Care Is Self-Care we are discussing everything related to libido and desire. Founder & host, Patty Brisben, is joined by members of the Foundation's Medical Board of Dire...
2021-04-2659 minSex Care is Self Care
Sex Care is Self CareSHE+ #1 Back to BasicsBefore we can talk about specific sexual health issues, we need to understand what sexual health is, why it’s important, and how sex positivity is related. Patty and the PBF Medical Advisory Board discuss these important topics, and delve into the 6 focus areas of the Foundation as well as a quick overview of our annual grant cycle. In our inaugural episode, your founder & host, Patty Brisben, is joined by members of the Foundation's Medical Board of Directors: Cheryl Iglesia, MD, FACOG, FPMRS; Michael L. Krychman, MDCM; Kathleen Novicki, PT, DPT, PRPC; and Christine Vaccaro, DO, FACOG, FPM...
2021-04-0744 minDr. Bri Talks Lady Bits
Dr. Bri Talks Lady BitsEp 61: Natural Support for Endometriosis, and Using Your Cycle as Your Superpower w/ NajaWe're wrapping up Endometriosis Awareness month, but as physical therapist and health coach Naja Chikazunga-Martin and I discuss, endometriosis doesn't end when March is over! It's an issue women deal with YEAR-ROUND. If you suffer from endo (or know someone who does), tune into this awesome conversation. It's not just for women with endo, but for any woman with a menstrual cycle. Naja shares: How we can use our cycle as our superpower... even with endo!  The importance of "remembering the wins" (remembering what's worked in the past) to retrain the brain and invoke a feeling of...
2021-03-3134 minThe Endo Babe Podcast: Endometriosis w/ Chelsea Bri
The Endo Babe Podcast: Endometriosis w/ Chelsea Bri65. Ways to Effectively Manage Painful Sex with Dr. Amanda Olson DPTIn this first episode of the Endo Empowerment Training Series, I have an awesome conversation with Dr. Amanda Olson, a pelvic floor physical therapist, about how helpful pelvic floor PT can be in managing painful sex for womxn with endometriosis! Stay tuned all month long for more of the Endo Empowerment Training Series! Dr. Amanda Olson holds a Doctorate in Physical Therapy, a Certification of Achievement in pelvic floor physical therapy (CAPP-PF) through the APTA, and the Pelvic Floor Practitioner Certification (PRPC) through the Herman and Wallace Pelvic Institute...
2021-03-0338 minOne Simple Step Today
One Simple Step Today"I don't want to tear!" Preparing your Pelvic Floor for delivery.Birth Preparation can help minimize tearing in delivery. In this episode learn about your anatomy, diaphragmatic breathing, perineal massage, and various birthing positions. Preparing for delivery can impact your experience. For more information, get Heather's book, Connecting With Your Body Throughout Pregnancy, available on Amazon or https://linktr.ee/onesimplesteptoday
2021-02-2511 minThe Don\'t Beat Around the Bush Podcast
The Don't Beat Around the Bush PodcastIntimate RosebushWe are so excited have DR. AMANDA OLSON Join us on the podcast for our VALENTINES DAY EPISODE Dr. Olson is the President and CCO of Intimate Rose as well as a CAPP-PF AND PRPC certified pelvic floor physical therapist We talk details of the life changing event that thrust Dr. Olson into pelvic floor PT and sparked her passion for creating the BEST pelvic floor devices on the market.  Pelvic Pain can really be a challenge for our intimate relationships and Dr. Olson also provides us with some amazing insights into involving o...
2021-02-1457 minOne Simple Step Today
One Simple Step TodayTraveler's ConstipationHave you suffered from constipation while on vacation? It's very uncomfortable. Understanding the causes and taking some intentional steps can relieve the constipation. In this episode, learn how movement, fiber, water and squatty potty can help counter constipation. For more information, go to www.onesimplestep.today
2021-02-0906 minAligned + Elevated with Dr. Allison Feldt
Aligned + Elevated with Dr. Allison FeldtEpisode 55 Pliability Health CoachingIn Episode 55 of The Edmonds Moms Room Dr. Allison Feldt, PT, DPT is joined by Dr. Kelsea Cannon, PT,DPT,PRPC,WHCowner of Pliability Health Coaching. Listen in as they discuss the amazing support that Dr. Kelsea provides new moms whether they're 2 weeks or 2 months postpartum! Pliability Health Coaching Helping Mom Move Safely Beyond Pain and Back Into Her Livelihood for more information head to https://www.pliabilityhealthcoaching.com/ Music: Good Times by Podington Bear is licensed under a Attribution-NonCommercial 3.0 International License.© Chad Crouch(P) Chad CrouchCOMPOSER: Chad CrouchPUBLISHER: Victrola D...
2021-02-0523 minOne Simple Step Today
One Simple Step TodayNighttime urination: Nocturia. How many times is normal?You've probably had those nights that you've lost track of how many trips to the bathroom! In this episode learn the healthy frequency, questions to ask yourself, and ways to delay the urge to urinate at night. For more information, go to: www.onesimplestep.today #onesimplesteptoday
2021-01-2508 minOne Simple Step Today
One Simple Step Today2nd Trimester: Symptoms, Changes, and what to expect.2nd trimester is months 4, 5, 6 in pregnancy. Often this is characterized by more energy compared to 1st trimester. In this episode, 2nd trimester symptoms such as round ligament pain, constipation, swelling, and sleeping positions are discussed. For more information, go to www.onesimplestep.today.
2021-01-0616 minThe Idaho Physio
The Idaho PhysioDr. Breann Fox, PT, DPT, PRPC: Health, Business, and Life Coaching - Dr. Fox helps accomplished women connect within, clear their paths and elevate their relationships!In the show we talk about: - How being a Doctor of Physical Therapy that specializes in pelvic health and visceral mobilization has helped her on her journey- Dr Fox takes her knowledge of PT and collaborates that with her studies/work around releasing trauma to help people get “un-stuck” and elevate to their potential!- She explains her “F-Bombs”!! - She talks about replenishing and recharging within so we can continue to give to oth...
2020-12-0834 minOne Simple Step Today
One Simple Step TodayLet's discuss Mammograms!Mammograms are usually ordered by your OBGYN after age 40. Knowing what to expect and how to prepare may ease your apprehension about having a mammogram. In this episode, I share my personal experiences about my recent 3D mammogram. Ladies, take care of yourself!
2020-12-0305 minDanceWell Podcast
DanceWell PodcastEpisode 76: Returning to Dance PostpartumWhat happens after your OB clears you to return to activity after approximately 6 weeks postpartum? Is it wise to take your first ballet class the day after clearance? As it turns out, things like prolapse, incontinence, pelvic floor dysfunction and pain are more common than we realize postpartum and require some attention in order to return to activity in full. In this episode, we are joined by Dr. Julia Rosenthal PT, DPT, OCS, PRPC who will talk briefly about a wide range of topics to consider when returning to dance postpartum including prolapse, urinary incontinence, the GI system, breastfeed and...
2020-11-181h 03One Simple Step Today
One Simple Step TodayStress Incontinence: causes, symptoms, tips and treatment.Stress Incontinence affects up to 1 in 3 women. The unwanted loss of urine can be embarrassing, frustrating and inconvenient. In this episode, we discuss the connection between the pelvic floor and incontinence symptoms and Kegels. Simple ways to improve your health. For more information, go to www.onesimplestep.today.
2020-11-1711 minOne Simple Step Today
One Simple Step TodayBladder Habits for Better Bladder Health.November is Bladder Health Awareness Month, so in this episode we will explore the 5 F's. Frequency, Flow, Fluids, Foods, and Freedom can impacts your bladder habits. You will learn how to be aware of your bladder habits and make improvements so you don't let your bladder control you. For more information, go to www.onesimplestep.today.
2020-11-0712 minOne Simple Step Today
One Simple Step TodayScar massage after C-section or Vaginal DeliveryScar massage is important after delivery as the body does a great job of healing. However, in postpartum, sometimes this can lead to pain due to decreased scar mobility. In this episode, the importance of scar massage is discussed and tips provided. For more information on women's health topics, go to www.onesimplestep.today.
2020-10-2007 minOne Simple Step Today
One Simple Step TodayI love coffee, but....what about my bladder?Love for coffee is real, but how does it affect your bladder and bowel health? In this episode, learn about how coffee affects you and some simple steps to enjoy your coffee and still be healthy! Go to www.onesimplestep.today for additional resources about bladder and bowel health.
2020-10-0108 minOne Simple Step Today
One Simple Step TodayFreezer Meal Tips for New MomsPreparing food for after delivery is important. Listen to this episode for freezer meal tips with ways to partial prep meals. Meat, veggies and fruits are discussed as well. For more information, visit www.onesimplestep.today
2020-10-0107 minThe Sweetest (and Toughest) Job
The Sweetest (and Toughest) JobPelvic Health and Tools that Can Help with Amanda OlsonPelvic dysfunction is a very common result after giving birth whether you deliver vaginally or via c-section but it is just not talked about enough! Dr. Amanda Olson is the President and Chief Clinical Officer of Intimate Rose where she develops pelvic health products and education and authored the book Restoring the Pelvic Floor For Women.  She is passionate about empowering women and men with pelvic health issues including pelvic pain, incontinence, and pre and postpartum issues. Amanda shares her shocking story of how she sustained an injury prior to having children which changed her mind about th...
2020-09-1731 minOne Simple Step Today
One Simple Step TodayWhat to wear to Pelvic Floor Therapy? What to expect at my first visit?What to wear is an important question when you are preparing for your first pelvic floor physical therapy appointment. Included in this episode are some tips for clothes and shoes. Additionally, what to expect at your first visit will have ease your fears and give you confidence.
2020-09-1008 minOne Simple Step Today
One Simple Step TodaySex after Baby| Why wait? What's happened to the pelvic floor?Sex After Baby, you may have questions and in this episode, answers and tips are discussed. Included are reasons why you need to wait, why is my sex drive decreased, what to do if there's pain with sex. Sex should be pain free and enjoyable. There's help available.
2020-09-1010 minOne Simple Step Today
One Simple Step TodayPostpartum Pelvic Floor Tips, 3 Things TO DO and 3 Things TO AVOID.Reconnecting to your pelvic floor in postpartum is so important. Remembering the basics is a great place to start, especially in the first 6 weeks. In this episode, you'll learn how to breathe and move with your pelvic floor. Also, avoid some activities to prevent hurting your pelvic floor.
2020-09-0509 minOne Simple Step Today
One Simple Step TodayPostpartum Anxiety | What You Need To KnowPostpartum Anxiety impacts 10% of New Moms. It can cause a great deal of emotional and physical pain. In this episode, we will explore postpartum anxiety, the causes, potential treatment options, and places for you to get help and support. Be sure to check out www.onesimplestep.today for additional resources to help you deal with Postpartum Anxiety.
2020-09-0210 minOne Simple Step Today
One Simple Step TodayHydration Doesn't Have To Be Boring | Tips To Stay HydratedAlmost everyone needs more water, but especially pregnant women. In this episode, we will talk about ways to make hydration and water consumption more enjoyable. Especially during pregnancy drinking enough water can be hard, so listen to this episode and get the encouragement and tips you need to keep hydrated.
2020-08-2809 minOne Simple Step Today
One Simple Step Today3 Tips for a Healthy Bladder!3 Tips for a Healthy Bladder! How are your peeing habits? These 3 questions are discussed: How often do you go? How full is your bladder? What color is your urine? Don't ignore your bladder habits. #onesimplesteptoday
2020-08-1804 minThe justASK Podcast
The justASK PodcastSeason 1:8 - Tools and Products for Pelvic Health with Amanda Olsen DPT, PRPCToday we're talking with pelvic health expert, Amanda Olson, Founder of IntimateRose about pelvic floor disorders such as incontinence, pelvic organ prolapse, pelvic pain, and pregnancy-related issues.From pain with sex to embarrassing urine leakage, and a show and tell of tools for better pelvic health, we're going to review it all!Have a question about pelvic health? Drop it in the comments or send it anonymously via messenger. No topic is off-limits. #justASK#justASK is a healthcare platform that is dedicated to providing evidenced-based information by a multidisciplinary team of sexual health...
2020-08-0154 minDown There Aware
Down There AwareWhy should I care about my pelvic floor?Continuing the conversation about pelvic floor health, Mary and Alex sit down with Dr. Amanda Olson. Dr. Olson earned her Bachelor of Science degree from Pacific University in 2005, and a Doctorate Degree in Physical Therapy from Regis University in 2008, graduating as a member of the Jesuit National Honor Society. She holds a Certification of Achievement in pelvic floor physical therapy (CAPP-PF) through the APTA, and the Pelvic Floor Practitioner Certification (PRPC) through the Herman and Wallace Pelvic Institute. She is also a certified Stott Pilates instructor and RRCA certified running coach, which she finds useful in curating treatment approaches. She...
2020-07-2728 minSluts and Scholars
Sluts and Scholars148 The Pelvic Whisperer with Dr. Sherine Aubert, PT, DPT, PRPCDr. Sherine Aubert talks all about pelvic health and how the pelvis influences many aspects of life on a deeply rooted level- mentally, physically and emotionally. Tune in for a chat about pelvic floor therapy for all bodies.   Old episode with Kayna Cassard on Pelvic Pain: https://slutsscholars.libsyn.com/101-is-a-tight-vagina-a-thing-pelvic-pain-with-kayna-cassard   Dr. Sherine: https://www.pelvichealing.com/team/sherine   Restored Physique: https://www.restoredphysique.com/meet-the-team   FOLLOW US  Twitter Instagram Facebook Send questions, comments, stories, rants to: SlutsAndScholars@gmail.com Sluts An...
2020-07-1140 minOne Simple Step Today
One Simple Step TodayChildbirth DoulaWhat is a Doula? Is a Doula and midwife different? In this interview we'll talk with Tiffany Hardegree, @tmhardegree, a Childbirth Doula to answer those questions and more. We'll explore: What is a Doula and what they do? How can a Doula help you if you are pregnant? We'll also provide pregnancy tips for working with a Doula and questions you should ask a Doula. Sit back and enjoy the interview and I hope by the end of this you can answer the question What is a Doula and what do they do! Visit my website for additional resources:   www.o...
2020-05-2720 minOne Simple Step Today
One Simple Step TodayTreatments for Heavy Bleeding During Periods | Endometrial Ablation for Heavy Bleeding? Personal ReflectionsMany women suffer from heavy bleeding during periods, in fact 1 in 3 women describe their periods as heavy.  There are many Treatments for Heavy Bleeding During Periods, one of those is Endometrial ablation. In this episode, I share my personal journey of choosing the treatment for my heavy bleeding.  I explain the decision process, procedure and recovery process. There are many treatment options.  Being informed, doing research, asking questions can help anyone make the best decision about their health.
2020-05-1008 minOne Simple Step Today
One Simple Step TodayTips for Relieving Constipation in PregnancyFrustrated by constipation? Over 25% of pregnant women deal with constipation.  In this episode, find 6 tips + bonus for finding relief from constipation.  In this episode we'll explore the causes of constipation and ways to relieve them during pregnancy.  I hope you find this episode helpful as you continue on your pregnancy journey. Be sure to like the episode if you found it helpful and share it with a friend!  For more resources and information: Visit my website for additional resources:  www.onesimplestep.today   Follow on FB - https://www.facebook.com/OneSimpleStep Follow...
2020-05-0510 minOne Simple Step Today
One Simple Step TodayPregnancy & Delivery During Covid-19 - Interview with Dr. Danielle KikoPregnancy during COVID-19 can create fear and uncertainty. In this episode, we interview an OBGYN, Dr. Danielle Kiko,  who answers your pregnancy and delivery questions as well as concerns about the coronavirus. This interview is here to educate and empower you. Be sure to check out Dr. Kiko's clinc at www.gwhobgyn.com and be sure to leave your comments or questions at www.onesimplestep.today or TWT @simpletoday or YouTube channel: One Simple Step Today. We will do our best to have more interviews, so be sure to submit your questions. 
2020-04-2526 minThe Modern Mommy Doc Podcast
The Modern Mommy Doc PodcastHow to Take Care of Your Pelvic Floor with Eryn Kirschbaum, PT, DPT, PRPCHaving a new baby can be stressful when it comes to mental health but also when it comes to your physical health. This week on the podcast, we’re talking to Eryn Kirschbaum, PT, DPT, PRPC about how to take care of your pelvic floor. We’re putting it all out there, discussing hemorrhoids, urinary incontinence, constipation. You name it, we’re covering it!--Our online parenting programs are HERE!! In need of some extra support with straight-talk, trustworthy, digestible information to level up your parenting? We’ve got you, mama! Covering four of the most...
2020-04-2042 minReal Talk with the Pelvic Docs
Real Talk with the Pelvic DocsVaginal Weights, Wands, & Dilators With Dr. Amanda OlsonWhat the heck do you do when your vagina is too tight and you are forced to shelter in place in the middle of a pandemic? Have no fear ladies...we have a solution. Pelvic Health Specialist and Owner/founder of Amanda Olson has got the products for you. We talk the full spectrum of medical grade vaginal trainers: vaginal weights, vaginal dilators, vaginal wands with vibration. Dr. Amanda Olson, PT, DPT, PRPC is a Doctor of Physical Therapy who specializes in treatment of pelvic floor disorders including incontinence, pelvic organ prolapse, pelvic pain, and pregnancy related i...
2020-04-1230 minReal Talk with the Pelvic Docs
Real Talk with the Pelvic DocsCompassionate Curiosity & Pelvic PainIn this episode, Joc and Jenny sit down with Dr. Susie Gronski to discuss persistent pelvic pain across all genders, including what it is and how it is treated. She offers concrete examples of intervention strategies as well as suggestions of things for individuals with pelvic pain to consider when seeking a health care provider. Hope you enjoy Dr. Susie G as much as we did! Dr. Susie Gronksi DPT, PRPC, WCS is a physiotherapist for your privates. She trains men and women on how to be their own expert in treating whatever's going on ‘down there.’ Check...
2020-02-2329 minOne Simple Step Today
One Simple Step TodayPooping HabitsPooping...we all do it, but we don't talk about it. What are good habits? What is harmful to your pelvic floor? Listen and learn helpful tips!
2020-01-2810 minIPTA
IPTA#THIRDTHURSDAY: PT Access: Pro Bono Physical Therapy Services in Illinois (January 2020)The APTA code of Ethics Principle #8 states that physical therapists shall participate in efforts to meet the health needs of people locally, nationally, or globally. (Core Value: Social Responsibility). Principle 8A goes on to say physical therapists shall provide pro bono physical therapist services or support organizations that meet the health needs of people who are economically disadvantaged, uninsured, and underinsured. The purpose of this #THIRDTHURSDAY lecture is to assist PTs and PTAs in understanding these responsibilities and learn how to become involved in local pro bono efforts. The presentation will define pro bono services and APTA’s...
2020-01-271h 09One Simple Step Today
One Simple Step TodayOne Simple Step Today (Trailer)
2020-01-2200 minPodcastDX
PodcastDXPre & Post Natal Pelvic Floor TherapyIn this episode we will discuss pre and post natal pelvic floor therapy with Erin Glace.   Erin Glace PT, MSPT, PRPC is a pelvic health specialist with over 20 years of experience treating women with pelvic floor problems.  Pelvic floor damage can occur during pregnancy, during childbirth or post-partum, and may be expressed by symptoms such as urinary incontinence, fecal and gas incontinence, sexual dysfunction, pelvic organ prolapse and chronic pelvic pain. Pelvic floor muscle training exercises are recommended for: Women with urinary stress incontinence Men with urinary stress incontinence after prostate surgery People who have fe...
2019-10-1534 minEveryday Family Medicine
Everyday Family MedicineIs Primary Care Taking Full Advantage of Physical Therapy? Host: Jennifer Caudle, DO Guest: Catherine Apicella, MPT, PYT-C, PRPC With less and less primary care physicians partnering with physical therapists, it’s time we ask: are we doing ourselves—and our patients—a disservice by not taking advantage of this option? To find out, Dr. Jennifer Caudle sits down with physical therapist Catherine Apicella to discuss the often-overlooked benefits of physical therapy and how primary care physicians and physical therapists can work together to benefit their patients.
2019-06-0700 minIPTA
IPTA#THIRDTHURSDAY: Physical Therapy & The Postpartum Woman (March 2019)This 90-minute seminar will introduce several concepts regarding the postpartum physical therapy patient. The instructors will introduce relevant history questions and possible red flags during the evaluation. Lumbo-pelvic-hip complex conditions related to this population will be reviewed, including common assessment and treatment techniques. This will include SIJ dysfunction, diastasis recti, and pelvic floor dysfunction. Cesarean scar management will be discussed, as well as common peripheral nerve injuries following pregnancy and delivery. The goal of this short presentation is to provide therapists with clinically relevant information useful in treating the postpartum client. What to expect:  Upon completion of t...
2019-04-181h 28Everyday Family Medicine
Everyday Family MedicineHow Pelvic Floor Physical Therapy Can Help MS Patients Host: Jennifer Caudle, DO Guest: Catherine Apicella, MPT, PYT-C, PRPC Frustrating multiple sclerosis (MS) symptoms like urinary frequency and incontinence can be detrimental to a patient’s quality of life, but there are resources available to help. To explore the benefits of pelvic floor physical therapy for MS, Dr. Jennifer Caudle sits down with two special guests: physical therapist Catherine Apicella from CORE 3 Physical Therapy and her patient—and ReachMD’s very own employee—Crystal Gouldey.
2019-03-1200 minFUMS: Giving Multiple Sclerosis The Finger
FUMS: Giving Multiple Sclerosis The FingerFUMS 011 - Sex, Constipation, Incontinence - Oh My!Erin Glace, MSPT, PRPC, BCB-PMD, is the clinical director of the Physical Therapy and Urodynamics departments at Urology of Virginia. She is a graduate of the University of Florida with her bachelor’s degree in Physical Therapy and a graduate of Old Dominion University with an advanced master’s degree in Physical Therapy. Her sole focus of treatment is pelvic floor dysfunction.She has a TON of initials behind her name for all of the training she's done and she's respected throughout her specialty and beyond. AND - she's one of my oldest and dearest friends! When I as...
2018-04-3036 minHealthy Wealthy & Smart
Healthy Wealthy & Smart231: Dr. Sarah Ecker: Addressing Pelvic Health in HaitiOn today's episode of the podcast, Sarah Ecker PT, DPT, PRPC joins me to discuss the STAND (Sustainable Therapy And New Development) Haiti Project which is a nonprofit organization working to provide continual, orthopedic care to Haitian communities most in need. Sarah is a physical therapist specializing in pelvic health and has been traveling the country as a "Travel Therapist," working in some of the United States' most impoverished communities. Sarah is STAND's Director of Pelvic Health and is continuing to help further the mission of STAND and ensure that women in Haiti have improved access to pelvic health...
2016-09-2641 minMedizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 17/19
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 17/19Einfluss der Kupferbindung an der Aminosäureposition 95 im Prionprotein auf den Krankheitsverlauf nach PrioninfektionPrionerkrankungen gehören zu den neurodegenerativen Erkrankungen und können sowohl genetisch übertragen als auch erworben werden. Sie verlaufen bisher ausschließlich letal. Verursacht werden sie durch das PrPsc, die pathologische Isoform des physiologisch vorkommenden PrPc. Ein – teilweise noch kontroverser – Einfluss von Kupfer auf die Pathologie wurde mehrfach dargestellt. Bisherige Arbeiten haben diesen Einfluss jedoch insbesondere anhand der Bindung an die Oktarepeatregion untersucht; zunehmend wächst allerdings das Interesse an den Kupferbindungsstellen außerhalb dieser Region: unter anderem insbesondere am Histidin 95 (His95). Im Rahmen dieser Arbeit wurde erstmalig der Einfluss der Kupferbindung an der murinen Aminosäure Histidin 95 des Prionproteins (PrP) in viv...
2014-11-2700 minMedizin - Open Access LMU - Teil 19/22
Medizin - Open Access LMU - Teil 19/22Cellular Prion Protein Mediates Toxic Signaling of Amyloid BetaPrion diseases in humans and animals comprise a group of invariably fatal neurodegenerative diseases characterized by the formation of a pathogenic protein conformer designated PrPSc and infectious particles denoted prions. The cellular prion protein (PrPC) has a central role in the pathogenesis of prion disease. First, it is the precursor of PrPSc and infectious prions and second, its expression on neuronal cells is required to mediate toxic effects of prions. To specifically study the role of PrPC as a mediator of toxic signaling, we have developed novel cell culture models, including primary neurons prepared from PrP-deficient mice. Using these approaches...
2012-01-0100 minFakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 03/06
Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 03/06Rhabdovirus Envelope-SwitchingDas Tollwutvirus (Rabies Virus (RV)) ist ein neurotropes Virus aus der Familie der Rhabdoviridae innerhalb der Ordnung Mononegavirales. RV Partikel werden an der Zelloberfläche durch Membran-Budding gebildet. Dabei wird der virale Ribonukleoproteinkomplex (RNP) durch das Matrixprotein (M) in eine Membranhülle verpackt in der Trimere des Transmembran-Glykoproteins (G) selektiv inkorporiert werden. Die G Spikes vermitteln die Bindung der Virionen an zelluläre Rezeptoren und die Fusion der viralen und zellulären Membran. Auch in Abwesenheit des G werden Viren freigesetzt, da der Budding-Prozess hauptsächlich durch das M Protein vermittelt wird. Das G-unabhängige Budding der Rhabdoviren erlaubt den Austau...
2008-12-0100 minTierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 03/07
Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 03/07Zum Vorkommen von Prion Proteinen in der Milchdrüse und der Milch von HauswiederkäuernDie vorliegende Arbeit untersuchte im ersten Teil tierart-vergleichend das Vorkommen von PrPC bei Hauswiederkäuern in Eutergewebe und in den verschiedenen Milchfraktionen. Dies erfolgte mittels EIA und Western-Blot sowie für das Eutergewebe auch mittels Immunhistochemie (IHC). Für die Aufarbeitung von Rahm wurde dabei ein neues Verfahren etabliert, durch das die Proteinfraktion des Rahms als Pellet gewonnen werden konnte. Es fand seinen Einsatz auch im zweiten Teil bei der Untersuchung von Milch und Kolostrum BSE- bzw. Scrapie-infizierter Schafe mittels PrionScreen® und Western-Blot. Auch das Eutergewebe und die Milchfraktionen von drei BSE-Kühen wurden untersucht. Im Eutergewebe vom Rind war PrPC...
2008-02-0800 minFakultät für Physik - Digitale Hochschulschriften der LMU - Teil 02/05
Fakultät für Physik - Digitale Hochschulschriften der LMU - Teil 02/05Molekulardynamik-Simulationen von amyloidogenen Proteinen in Lösung: Stabilitätsuntersuchungen und Weiterentwicklung einer KontinuumsmethodeViele neurodegenerative Erkrankungen, wie die transmissiblen spongiformen Enzephalopathien (TSE), die Alzheimer- und die Huntington-Krankheit, sind durch charakteristische Ablagerungen im Gehirn, sogenannte Amyloide, gekennzeichnet. Amyloide sind oftmals fibrilläre Aggregate von normalerweise löslichen Proteinen, deren dreidimensionale Strukturen sich bei der Aggregation verändern. Bedauerlicherweise waren hochauflösende Methoden biophysikalischer Strukturaufklärung bislang auf Amyloide nicht anwendbar. Dagegen können Molekulardynamik (MD)-Simulationen amyloidogener Proteine und Peptide in ihrer Lösungsmittelumgebung dazu beitragen, die Mechanismen der auftretenden Konformationsänderungen zu verstehen und die Strukturen amyloider Fasern aufzuklären. Die korrekte und effiziente Beschreibung der Lösungsmittelumgebung spielt dabei eine entscheidende Rolle. Im ersten...
2007-02-0700 minTierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 02/07
Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 02/07Zum Nachweis von pathogenen Prionen mittels "Kapillarelektrophorese-laserinduzierter Fluoreszenz (CE-LIF)"Zum Nachweis von PrPc und PrPsc wurde eine Kapillarelektrophorese-Immunofluoreszenz-Methode (CE-LIF) etabliert und mit konventionellen Verfahren (ELISA, Westernblot, Dot-EIA) verglichen. Zur CE-LIF wurde der monoklonale Antikörper F89 und ein Fluoreszenz-markiertes Peptid (FITC-P3) eingesetzt. Die Nachweisgrenze für rekombinantes bovines PrPc liegt bei 1 pg/Test. Zur Anreicherung von PrPsc aus Hirn und Liquor wurden (Ultra-) Zentrifugations-, Extraktions- (HFIP) und Chromatographieverfahren (HPLC, Festphasenextraktion) einzeln oder in Kom-binationen geprüft; darüber hinaus wurden Anreicherungsversuche mit Affinität- bzw. Immu-nomagnetischer Separation vorgenommen. Die Überprüfung des PrPsc-Gehaltes ausgewählter Extrakte im Vergleich zum Ausgangsma-terial mittels ELISA ergab deutliche Substanzverluste. Eine selektive Bindung von PrPsc an...
2006-07-2800 minMedizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 05/19
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 05/19Amplifikation von Prionen in vitroDie Arbeit „Amplifikation von Prionen in vitro“ beruhte auf der Amplifikationsmethode die erstmals 2001 vorgestellt wurde (Saborio et al., 2001). Bei der PMCA wird erstmals PK-resistentes PrPres in ausreichender Menge in vitro hergestellt, welches molekularbiologisch dem Erreger spongiformer Enzephalopathien gleicht. Die PMCA erlaubt somit eine in vitro Untersuchung des pathologischen Umfaltungsprozess von PrPC zu PrPSc für diagnostische und therapeutische Studien. In dieser Arbeit wurden ausgiebig die Einzelschritte der PMCA Methode, insbesondere die Quantifikation der Western Blot Bande und die Auswirkungen der Sonifikationsleistung und der Inkubationszeit auf die Amplifikationseffizienz untersucht. Je nach Stärke der Sonifizierung ändert sich die Effizienz der PMCA Reaktio...
2006-07-0600 minFakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 02/06
Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 02/06Molekulare Mechanismen der PrionpropagationEin zentrales Ereignis in der Pathogenese der Prionkrankheiten ist die strukturelle Konversion des zellulären Prionproteins (PrPC) in eine infektiöse Isoform (PrPSc). Im Rahmen der vorliegenden Arbeit wurde die von der Prion-Hypothese angenommene Eigenschaft von PrPSc zur autokatalytischen Vermehrung durch serielle PMCA-Reaktionen experimentell belegt und die Vermehrung von Infektiösität in vitro dargestellt. Die Fragmentierung der PrP-Aggregate in kleinere Aggregationskeime hat sich dabei für eine effiziente autokatalytische Prion-Replikation als essentiell erwiesen. Diese Ergebnisse sprechen für die Richtigkeit des „seeded polymerisation“-Modells der PrPSc-Bildung und liefern einen experimentellen Beleg für die Prion-Hypothese. Die Übertragbarkeit von Prionkrankheiten auf andere S...
2006-04-1100 minFakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 02/06
Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 02/06The 37kDa/67kDa laminin receptor as a therapeutic target in prion diseases: potency of antisense LRP RNA, siRNAs specific for LRP mRNA and a LRP decoy mutantPrion diseases are a group of rare, fatal neurodegenerative diseases, also known as transmissible spongiform encephalopathies (TSEs), that affect both animals and humans and include bovine spongiform encephalopathy (BSE) in cattle, scrapie in sheep, chronic wasting disease in deer and elk and Creutzfeldt-Jakob disease (CJD) in humans. TSEs are usually rapidly progressive and clinical symptoms comprise dementia and loss of movement coordination. A common hallmark of TSEs is the accumulation of an abnormal isoform (PrPSc) of the host-encoded prion protein (PrPc) in the brains of affected animals and humans. PrPc is a highly conserved cell surface sialoglycoprotein that is expressed...
2006-04-0600 minFakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 02/06
Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 02/06Single chain antibodies against the 37 kDa/67 kDa laminin receptor as tools for prion diseases therapyPrions are unconventional pathogens that cause transmissible spongiform encephalopathies (TSEs). According to the "protein only" hypothesis, prions consist of an infectious protein that is capable of converting a normal host protein termed PrPc into a protease resistant form termed PrPSc. PrPSc is poorly degraded by the host and accumulates in the CNS. Normal biological functions of PrPc and mechanisms involved in neurodegeneration remain obscure. During the past two decades, considerable efforts have been made to elucidate prion diseases and in particular to identify PrP interactors for a better understanding in prion biology. A major break-through was the identification of the 37...
2006-01-2400 minMedizin - Open Access LMU - Teil 14/22
Medizin - Open Access LMU - Teil 14/22Brain-derived proteins in the CSF, do they correlate with brain pathology in CJD?Background: Brain derived proteins such as 14-3-3, neuron-specific enolase (NSE), S 100b, tau, phosphorylated tau and A beta(1-42) were found to be altered in the cerebrospinal fluid (CSF) in Creutzfeldt-Jakob disease (CJD) patients. The pathogenic mechanisms leading to these abnormalities are not known, but a relation to rapid neuronal damage is assumed. No systematic analysis on brain-derived proteins in the CSF and neuropathological lesion profiles has been performed. Methods: CSF protein levels of brain-derived proteins and the degree of spongiform changes, neuronal loss and gliosis in various brain areas were analyzed in 57 CJD patients. Results: We observed three...
2006-01-0100 minFakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 02/06
Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 02/06Biogenese des Prion-ProteinsPrion-Erkrankungen sind tödlich verlaufende, neurodegenerative Erkrankungen, die sporadischen, hereditären oder infektiösen Ursprungs sein können. Die Missfaltung des zellulären Prion-Proteins (PrPC) spielt eine zentrale Rolle bei der Pathogenese. In der vorliegenden Arbeit wurden zwei verschiedene Themenbereiche bearbeitet: zum einen die Analyse der Faltung und Maturierung krankheitsassoziierter Mutanten und zum anderen die Wirkung einer Anti-Prion-Substanz auf die Biogenese von PrPC und die Propagierung von infektiösem PrPSc. Hierfür wurden verschiedene humanpathogene Mutanten, sowie andere Punkt- und Deletionsmutanten in Maus-Neuroblastomzellen untersucht. Im ersten Teil dieser Arbeit konnte gezeigt werden, dass zwei Mutationen, die Prion-Erkrankungen beim Menschen hervorrufen (T183A u...
2005-10-2500 minMedizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 04/19
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 04/19Polarisierter Transport des Prion-ProteinsSeveral proteins linked to neurodegenerative diseases, such as the -amyloid precursor protein, amyloid -peptide, -secretase, and tau, undergo selective polarized sorting. We investigated polarized sorting of the mammalian prion protein (PrPC) and its homologue doppel (Dpl). In contrast to Dpl, which accumulates on the apical surface, PrPC is targeted selectively to the basolateral side in Madin-Darby canine kidney cells. An extensive deletion and domain swapping analysis revealed that the internal hydrophobic domain (HD) of PrP (amino acids 113–133) confers basolateral sorting in a dominant manner. PrP mutants lacking the HD are sorted apically, while Dpl chimeras containing the HD of PrP ar...
2005-07-1900 minTierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 02/07
Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 02/07Entwicklung und Anwendung eines enzymimmunologischen Verfahrens zum Nachweis von zellulärem Prion Protein bei WiederkäuernDie vorliegende Arbeit beschäftigt sich mit der Entwicklung eines Enzymimmuntests (EIA) zum Nachweis von zellulärem PrP (PrPC), sowie dessen Anwendung zur Untersuchung diverser Gewebe von Rindern, Kälbern und Lämmern. Mit dem Testverfahren wurde auch Milch von Rindern, Ziegen und Schafen auf das Vorkommen von PrPC und dessen Verteilung auf die einzelnen Milchfraktionen untersucht. Bei der Untersuchung der Gewebsproben zeigte sich, dass Prion Proteine in nahezu jedem Gewebe nachweisbar sind. Neben dem Stammhirn sind auch andere Organe bzw. Gewebe, wie beispielsweise die Nickhaut der Lämmer oder die Lunge von Kälbern und Lämmern sowie die Nierenr...
2005-07-1500 minMedizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 02/19
Medizinische Fakultät - Digitale Hochschulschriften der LMU - Teil 02/19The subcellular trafficking of the prion protein: Characterisation of the function of the PrPc N-terminusTransmissible spongiform encephalopathies (TSEs) are degenerative diseases of the central nervous system in humans and animals, and include Creutzfeldt-Jakob disease (CJD) in humans, scrapie in sheep and bovine spongiform encephalopathy in cattle. These spongiform encephalopathies can manifest as sporadic, familial and acquired disorders and are caused by the conformational alteration of the non-pathogenic cellular prion protein (PrPc) into a infectious isoform denoted PrPSc. The latter therefore represents a pathogenic agent (prion) which does not contain nucleic acids. During biogenesis, PrPc undergoes posttranslational modifications with the addition of two N-linked carbohydrate chains and a glycosyl-phosphatidyl-inositol-(GPI)-anchor. Properly folded PrPc transits...
2003-09-2600 minFakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 01/06
Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 01/06The role of the 37-kDa/67-kDa laminin receptor in the cellular metabolism of the prion proteinPrionen Erkrankungen sind neurodegenerative Erkrankungen, bei denen die abnormale Form (PrPSc) des zellulären Prion Proteins (PrPC) eine entscheidente Rolle spielt. PrPSc lagert sich im Gehirn von Menschen und verschiedenen Säugetieren zu langen Ketten, sogenanntem Amyloid, zusammen und bildet amyloide Plaques. Dies führt letzendlich zum Tod des Individuums. Die Pathogenese und die zelluläre Funktion des Prion Proteins ist jedoch noch nicht vollständigig verstanden. Es wird vermutet, daß PrPC, sowie PrPSc mit verschieden Makromolekülen interagieren. In dieser Dissertation wird die Interaktion des 37-kDa/67-kDa Laminin-Rezeptor (LRP/LR) mit dem Prion Protein beleuchtet. Dabei konnte gezeigt werden, das bei...
2003-02-1100 minFakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 01/06
Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 01/06Identifizierung und Charakterisierung von Interaktoren des zellulären Prion-ProteinsDas Prion-Protein ist in seiner infektiösen Form für das Auftreten und die Übertragung von transmissiblen spongiformen Enzephalopathien verantwortlich. Diese Erkrankungen können bei Mensch und Tier auftreten, wobei die bekannteste tierische Form der „Rinderwahn“ bzw. BSE ist. Die häufigste Prion-Krankheit beim Menschen ist die Creutzfeldt-Jacob-Krankheit, deren neue Variante im Zusammenhang mit dem Auftreten von BSE steht. Die pathologische Form des Prion-Proteins (PrPSc) wird durch posttranslationale Umwandlung aus der apathogenen physiologischen Isoform PrPC gebildet. Dieses Protein wird vor allem in neuronalem Gewebe exprimiert und ist in allen Säugetieren hoch konserviert. Die Funktion des zellulären, apathogenen Prion-Proteins ist noch im...
2002-11-1100 minFakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 01/06
Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 01/06Characterization of the 37-kDa/67-kDa laminin receptor as the cell surface receptor for the cellular prion proteinPrions have been extensively studied since they represent a new class of infectious agents in which a protein, PrPSc (prion scrapie), appears to be the sole component of the infectious particle. They are responsible for transmissible spongiform encephalopathies (TSEs), which affect both, humans and animals. Human prion diseases occur in infectious, sporadic or genetic forms. The "protein only" hypothesis argues that the key event in the pathogenesis represents the conversion of the normal host protein, PrPc, into its pathogenic isoform PrPSc. Prion diseases have been associated with the accumulation of this abnormally folded protein and its neurotoxic effects. However, it...
2002-03-2500 minFakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 01/06
Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 01/06Neuropeptid Y- und Prionprotein- spezifische AptamereDie LQYLWUR Selektion ermöglicht es aus kombinatorischen Nukleinsäurebibliotheken Oligonukleotidsequenzen zu identifizieren, die verschiedenste Zielmoleküle mit hoher Affinität und Spezifität binden können. Dadurch haben sich Aptamere zu einer potenten Alternative zu den in der Diagnose, Therapie und als Forschungsreagentien etablierten Antikörper entwickelt. Mit Hilfe der SELEX-Technologie (6ystematic (volution of /igands by (;ponential Enrichment) ist es in dieser Arbeit gelungen, 2' amino-stabilisierte RNA-Aptamere gegen das Neuropeptid Y und ein ausgewähltes, funktionell relevantes Prionproteinepitop zu generieren. Die Anreicherung funktioneller Sequenzen erfolgte durch einen affinitätschromatographischen Prozess. Zudem sollten bereits vorliegende RNA-Aptamere, die gegen das rekombinante Prionprot...
2001-06-2000 min