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Echo PodcastyHanč: Babiš vyhrál, neudělal chybu. TOP 09 je v klinické smrti, program AntiBabiš se už vyčerpalProgram AntiBabiš se už vyčerpal. Pro SPOLU to byly volby bez tématu, do popředí se dostal Filip Turek, nakonec mu to pomohlo. Dostálová debaty v televizi zvládla a podpořil ji Andrej Babiš. Babiš dává mnohem větší důraz na estrádnost, ale po práci pro něj se mi nestýská, říká Marek Hanč, marketingový odborník, který byl mnoho let členem marketingového týmu Andreje Babiše.
Babišův elektorát prý ani nemusí vnímat, že se nějaké volby dějí, on nekandiduje, tak to pro ně není téma. Vystoupením v televizní d...2024-06-1114 min
Life sciences knowledge centerLyophilised Formulation DevelopmentThe properties of high stability, easy to storage and transport make lyophilization a suitable alternative to liquid formulations, especially for highly thermolabile products and live virus vaccine products.2022-03-0608 minLife sciences knowledge centerAutoimmune Disease VaccinesPreventive vaccines are for healthy people and prevent them from getting sick, which has been a great success. In addition to preventive vaccines, therapeutic vaccines are becoming increasingly important, especially as life expectancy increases. Vaccines for diseases such as AIDS, cancer, tuberculosis, hepatitis, Alzheimer's disease, and mad cow disease have not yet reached the stage of successful use. However, the vaccines in the field of autoimmune diseases have made significant progress. 2022-03-0609 minLife sciences knowledge centerHPV VaccinesHPVs are non-enveloped, double-stranded DNA viruses, which are tropic for mucosal tissues. The genome consists of three functionally divided regions: (a) a noncoding regulatory region; (b) an early proteins region, which encodes for six early proteins (E1, E2, E4-E7); and (c) a late region, which encodes for the viral capsid proteins L1 and L2. HPVs infect basal epithelial (skin or mucosal) cells. More than 200 HPV genotypes have been identified. Oncogenic genotypes include HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 66.2022-03-0612 minLife sciences knowledge centerParasitic VaccinesIn order to develop commercial vaccines against economically important parasites, the researchers have focused on identifying target antigens so far. Some of these involve ticks salivary gland antigens, secretory and excretory antigens of helminthes and hidden antigens of Heamonchus contortus.2022-03-0612 minLife sciences knowledge centerViral VaccinesViral Vaccines contain either attenuated (alive but cannot cause disease) viruses or inactivated viruses. Attenuated or live vaccines include the live form of the virus. These viruses are not pathogenic but can induce an immune response. Inactivated or killed viral vaccines include viruses that have lost their ability to replicate. To cause immune reaction, it contains more antigen than live vaccines.2022-03-0609 minLife sciences knowledge centerPlant-based VaccinesPlant-based vaccines are a kind of recombinant vaccines that introduce antigens against particular pathogens into the selected plant.2022-03-0612 minLife sciences knowledge centerCholangiocarcinoma VaccinesCholangiocarcinoma, also known as bile duct cancer, is a type of cancer consisting of a mutant epithelial cell (or a cell that exhibits epithelial differentiation). Cholangiocarcinoma originates in the bile ducts that excrete bile from the liver into the small intestine.2022-02-0812 minLife sciences knowledge centerGallbladder Cancer VaccinesGallbladder cancer is a relatively rare cancer. It is thought to be associated with the building up of gallstones and may also cause gallbladder calcification, a situation known as porcelain gall bladder, which is also rare.2022-02-0810 minLife sciences knowledge centerLiver Cancer VaccinesLiver cancer is a major health problem, with more than 850,000 new cases annually worldwide. This neoplasm is currently the second leading cause of cancer-related death globally. Among all primary liver cancers, hepatocellular carcinoma (HCC) is the most common neoplasm, accounting for approximately 90% of cases.2022-02-0809 minLife sciences knowledge centerPancreatic Cancer VaccinesPancreatic cancer is a type of cancer arising from cells in the pancreas (a glandular organ behind the stomach) when they begin to run out of control and become lumps.2022-02-0812 minLife sciences knowledge centerGastric Cancer VaccinesGastric cancer, also known as stomach cancer, is a cancer that develops from the lining of the stomach. Gastric cancer is the fifth leading cause of cancer in the world, the third leading cause of death from cancer.2022-02-0812 minLife sciences knowledge centerEsophageal Cancer VaccinesEsophageal cancer is a cancer arising from the esophagus, which is a food pipe runs between the throat and stomach. 2022-02-0808 minLife sciences knowledge centerGastrointestinal CancersGastrointestinal cancer refers to the malignant conditions of the gastrointestinal tract and digestive glands, including the esophagus, stomach, biliary tract, pancreas, small intestine, large intestine, rectum, and anus. 2022-02-0812 minLife sciences knowledge centerVaginal Cancer VaccinesVaginal cancer is a cancer that forms in the tissues of the vagina. There are two primary types of vaginal cancer: squamous-cell carcinoma and adenocarcinoma. 2022-02-0808 minLife sciences knowledge centerEndometrial Cancer VaccinesEndometrial cancer is the most common gynecologic malignancy in developed countries. Preclinical and clinical investigation of immunotherapy for endometrial cancer is not as advanced as for ovarian or cervical cancer. 2022-02-0808 minLife sciences knowledge centerOvarian Cancer VaccinesEpithelial ovarian cancer (EOC) accounts for 140,000 deaths annually worldwide and is the leading cause of gynecologic cancer-related mortality in the United States. Approximately, 70% of EOC patients present with advanced disease, and although the majority will respond to surgery and first-line chemotherapy, most of these responses are not durable: more than 90% of suboptimal surgically debulked patients and 70% of optimally debulked patients will relapse in 18 to 24 months. The major subtypes of ovarian carcinomas include high-grade serous carcinoma (HGSC), endometrioid carcinoma (EC), clear cell carcinoma (CCC), low grade serous carcinoma (LGSC), and mucinous carcinoma (MC). More recently, four molecular subtypes (C1/mesenchymal...2022-02-0808 minLife sciences knowledge centerGLP In Vivo ADME & PK StudiesThorough characterization and in-depth study of PK, as well as absorption, distribution, metabolism, and excretion (ADME) properties, are critical in order to support vaccine discovery and development processes for the production of safer and more effective vaccines.2022-01-0310 minLife sciences knowledge centerGLP Reproductive Toxicology StudiesReproductive toxicity is a special part of toxicology which comprises any effect of vaccine components on reproduction and development (embryonal and fetal development). The investigations and the interpretation of the results should be related to all other pharmacological and toxicological data available to determine the risk of the test vaccines to humans. 2022-01-0312 minLife sciences knowledge centerVaccine Assessment In Vivo AssaysIn vivo assays play a crucial role in the assessment of the potency and safety of human vaccines. Efficient translation from basic research to clinical therapies will depend on the availability of appropriate animal models to address every problem that arises during vaccine development.2022-01-0309 minLife sciences knowledge centerStability Tests of VaccinesThe real-time stability test of vaccines. It is to observe and study the physical, chemical, biological characteristics and change of efficacy of the vaccine over a period of time under the expected conditions of use and preservation. Accelerated stability study is the rate of change in physical, chemical and biological characteristics of a vaccine that is placed at sequence temperature including above the actual preservation temperature. The study provides supporting data on the shelf-life or release criteria for vaccines.2022-01-0312 minLife sciences knowledge centerPotency Test Service of Influenza VaccinesIt is currently determined that the key target of the protective immune response triggered by influenza vaccine immunization is hemagglutinin (HA), and neuraminidase (NA) may also play a role.2022-01-0312 minLife sciences knowledge centerPotency Tests of VaccinesThe Alternative Methods of Vaccine Potency Tests
The alternative methods include experimental animal substitution and serological substitution. The first method is to use other animals with similar clinical symptoms to replace the original animals, and indirectly test the efficacy of this animal vaccine. As for serological method, the efficacy of vaccine is assessed by detecting the antibody titer level after immunization. It involves viruses neutralizing experiments and all kinds of ELISA.2022-01-0312 minLife sciences knowledge centerMicrobial Tests of VaccinesOne of the most important effects of microorganisms on humans is to cause infectious diseases. Great advances have been made in the use of vaccines for disease prevention and intervention, but microbial infections continue to occur in vaccines. Therefore, it is very important to establish a standard vaccine microorganism detection platform.2022-01-0312 minLife sciences knowledge centerPurity Tests of VaccinesThe combination of chromatography with precipitation, centrifugation, and other traditional separation technologies has gradually become the main method of our vaccine separation and purification.2022-01-0312 minLife sciences knowledge centerIdentity Tests of VaccinesSince the vaccine is used in a large number of healthy persons, the adverse reaction after vaccination should be lower than the drug normally used for treatment. The identity tests for vaccines should be high standard.2022-01-0312 minLife sciences knowledge centerVaccine Assessment In Vitro AssaysPreventive vaccines are usually administered to healthy humans. Ensuring their safety and efficacy is therefore very crucial. Preclinical assessment is a prerequisite for the transfer of candidate vaccines from the laboratory to the clinic, including all aspects of animal testing, product characterization, proof of concept/immunogenicity studies, and safety testing prior to human clinical trials.2022-01-0312 minLife sciences knowledge centerCell Based VaccinesCell based vaccine is a vaccine developed from mammalian cell lines rather than embryonic chicken eggs. Cell culture techniques are used as a complementary and alternative platform for current egg-based strategies. Potential applications in the development of viral vaccines, particularly influenza vaccine, have been extensively studied in recent years.
2021-11-2909 minLife sciences knowledge centerBacterial Vector Vaccine DesignThe strategy of using live bacterial cells as vehicles to deliver recombinant antigens has emerged over the past 20 years as an interesting alternative for the development of new vaccines. With the development of genetic engineering techniques, recombinant microorganisms and pathogens are capable of expressing heterologous proteins in different cell compartments, thereby increasing their antigenic potential for vaccines against viruses, bacteria, and parasites.2021-11-2912 minLife sciences knowledge centerDNA and RNA Vaccine DesignNucleic acid vaccines were introduced decades ago but have already been widely used in infectious and malignant diseases. Nucleic acid vaccination is a technique for protecting against disease by injection with genetically engineered DNA (as a plasmid) or RNA (as mRNA). In contrast to recombinant bacteria or virus vaccines, nucleic acid vaccines consist only of DNA or RNA, which is uptake by cells and transformed into protein.2021-11-2908 minLife sciences knowledge centerHapten Conjugate Vaccine DesignA conjugate vaccine consists of a polysaccharide antigen that is conjugated to a carrier molecule. The conjugate vaccine is produced by covalently linking the poor antigens to the strong antigens, eliciting a stronger immune response to the poor antigen. This enhances the effectiveness and stability of the vaccine. Poor antigens are usually polysaccharides that are attached to strong protein antigens.2021-11-2912 minLife sciences knowledge centerNon-internalizing ADCNon-internalizing ADCs have the ability to induce a potent anticancer activity in vivo when used with a suitable payload and may target a broad variety of different malignancies. At present, they mainly rely on labile linkers for drug release in close proximity to the target because the intact ADC cannot passively diffuse into cells. Sufficient experimental data prove the fact that collagen IV, tenascin-C, fibrin, the alternatively spliced extra domains A and B (EDA and EDB) of fibronectin and galectin-3-binding protein (Gal-3BP) are appealing targets for non-internalizing ADC development. Scientific findings offer a preclinical proof-of-concept for curative...2021-11-2811 minLife sciences knowledge centerHydra LinkerBranched linkers (Hydra Linkers) are featured by their multiple payload loading capacity. They are bi-functional linkers that contain a main stem with a functional group at one terminus and a “branching point” at the other end. 2021-11-2811 minLife sciences knowledge center3D Cell Culture3D cell culture is a range of techniques that grow cells into 3D structures. Driven by the desire of biochemical and biomedical scientists for experimental systems that better represent the in vivo environments, new materials and methods for 3D cell culture has been developed. Application of 3D cell culture has resulted in better data and innovative knowledge of tissue and cancer behavior.2021-11-2811 minLife sciences knowledge centerPtLnX™: Pt-based ConjugationPtLnX is a technical platform developed by Creative Biolabs and one of our collaberators that uses bis(ethylenediamine) platinum chloride [Pt(en)Cl2] as a linker core for ADC development.2021-11-2811 minLife sciences knowledge centerQuenchbody DevelopmentQ-body is a new type of antibody-based probes that are highly innovative, versatile, and sensitive in detecting their corresponding antigens. It is constructed based on the principle of antigen-mediated fluorescent signal release. A Q-body is comprised of a fragment antibody (Fab or scFv) that is covalently modified by a fluorophore whose signal is, in the absence of the corresponding antigen, quenched by tryptophan (Trp) residues in the antibody. Upon antigen interaction, due to the change in antibody conformation, the Trp quenching effect is levitated, releasing the fluorescent signals. In principle, any antibody can be converted into a Q-body with...2021-11-2811 minLife sciences knowledge centerChimtoximAb PlatformCurrently, the conjugation strategies used to join payload and a guidance chimera are similar to those used in developing full-length Ab-based immuno-conjugates. To develop a ChimtoximAb, the designer often utilizes the exogenous effector moieties and antibody fragment molecules to create the conjugate. ChimtoximAb development is trending in terms of discovering the next-generation, functionally enhanced immuno-therapeutics with fragment antibodies as well as Fc-fusion proteins.2021-11-2810 minLife sciences knowledge centerADC Development for Urothelial TumorWith seven approved antibody-drug conjugates (ADCs) and over 50 others in clinical development, significant advances have been made in recent years to bring ADC technology to the forefront of drug development.2021-11-0811 minLife sciences knowledge centerADC Development for OsteosarcomaOsteosarcoma represents the most frequent malignant primary bone tumor, which mainly occurs in children and adolescents and causing high mortality. To date, despite significant advancements in the diagnosis and treatment of the cancer, long-term survival in both localized and metastatic osteosarcoma is still stagnated. Thus, conventional therapy is not fully adequate for osteosarcoma treatment and novel therapeutic approaches are required to improve outcomes.2021-11-0815 minLife sciences knowledge centerADC Development for Prostate CancerProstate cancer is one of the most common cancers in men. The standard treatment for patients with metastatic castration-resistant prostate cancer (mCRPC) have been assessed but failed to improve the overall survival (OS). Currently, novel treatment methods such as the targeted antibody-drug conjugates (ADCs) have been found to significantly improve the progression-free survival and OS rates.2021-11-0809 minLife sciences knowledge centerADC Development for Pancreatic CancersPancreatic cancer represents one of the most lethal solid tumors, which is notoriously refractory to chemotherapy. Thus, the therapy outcome for patients with pancreatic cancer is still poor. A large number of new treatment approaches are being investigated actively, and antibody-drug conjugates (ADCs) showing unique advantages has been widely assessed in the treatment of pancreatic cancer.2021-11-0810 minLife sciences knowledge centerADC Development for Multiple MyelomaIntroduction of Multiple Myeloma
MM is a relatively rare hematologic disorder characterized by proliferation of plasma cells, primarily involving the bone marrow. MM accounts for approximately 1% of all malignancies and approximately 10% of hematologic neoplasms. MM may present with elevated Calcium levels, renal insufficiency, anemia, and bone lesions as well as elevated levels of serum M protein or urine M protein or both. MM has 2 clinical variants: solitary bone plasmacytoma and extramedullary plasmacytoma. The diagnosis of MM is predominantly based on osteolytic bone destruction, plasma cell infiltration of bone marrow, hypercalcemia, anemia, and chronic renal insufficiency. A plain...2021-11-0810 minLife sciences knowledge centerEvaluation of Expression SystemThe development of highly efficient expression system is important to the manufacture of vaccines. Vaccine production may require relatively small quantities of expressed material but represent a very diverse category of different target antigens with significant structural differences. Thus, vaccine production requires production systems with very large outputs, which drives the quest for efficient and cost-effective systems.2021-11-0708 minLife sciences knowledge centerSqualeneSqualene is a squalene-based oil-in-water nano-emulsion with a formulation similar to MF59 that has been licensed in Europe for adjuvanted flu vaccines. Squalene is an oil more readily metabolized than the paraffin oil used in Freund's adjuvants. Squalene oil-in-water emulsion elicit both cellular (Th1) and humoral (Th2) immune responses.2021-11-0710 minLife sciences knowledge centermRNA VaccineThe mRNA vaccine field, which represents a promising alternative to conventional vaccine approaches, is developing extremely rapidly because of their high potency, potential for rapid development and capacity for low-cost manufacture and safe administration2021-11-0711 minLife sciences knowledge centerElectrophilic Vaccines (E-Vaccines) Platform for HIVElectrophilic Vaccines (E-Vaccines) Platform for HIV
Developed a method to stimulate B cells by covalently binding an immunogen containing a strong electrophilic phosphonate group to the native nucleophilic site of antibodies.2021-11-0712 minLife sciences knowledge centerHBV VaccinesHepatitis is a general term for liver inflammation, caused by infectious or non-infectious factors. Hepatitis B virus (HBV) infection is the main cause of morbidity and mortality. Hepatitis B is an infectious liver disease that comes from hepatitis B virus infection. Acute infection may last for several weeks. If the virus stays in the body, it can cause severe chronic infections, leading to liver damage, liver cancer and death. But Hep B infection can be prevented. The current guidelines recommend high-risk populations for chronic HBV infection screening so that diagnosed individuals can carry out your proper hepatitis care.2021-11-0709 min
Life sciences knowledge centerA brief introduction to ADCA brief introduction to ADC.2021-10-1810 min
comVaccine Development & GMP Manufacturing - Creative BiolabsWhat is a vaccine? Vaccine is a kind of biological agent that provides advance protection for future infections by stimulating and strengthening the receptor’s own immune system. Vaccines provide highly effective control of infectious diseases, and have been recently extended to applications in prevention and treatment for cancer, autoimmune diseases and other disorders. It is very important to develop and manufacture high-efficient and safe vaccines for biopharmaceutical companies. https://www.creative-biolabs.com/vaccine/vaccine-development-service.htm2020-11-0508 mincomNano Adjuvant - Creative BiolabsOil Adjuvants, Saponin Adjuvants, Lipopolysaccharide, molecules known to activate pattern recognition receptors (PRRs), referred to as PRR agonists and so on. Immunologic Adjuvants, which are added to vaccines, plays an important part in enhancing the immune response to the antigen. Adjuvants enhance the ability of antigens by boosting it to give a higher amount of antibody and a longer-lasting protection, thus minimizing the amount of injected foreign material. https://www.creative-biolabs.com/vaccine/category-adjuvants-5.htm2020-11-0510 mincomHIV vaccine - Creative BiolabsHIV is an abbreviation for human immunodeficiency virus, a lentivirus that causes acquired immunodeficiency syndrome (AIDS), which is a disease that gradually damages the body's immune system, and patients eventually die from various pathogen infections and cancer. In the absence of treatment, HIV-infected patients typically have a survival time of 9-11 years. HIV is a sexually transmitted virus that can be transmitted through contact with or transfer of the blood or body fluids such as semen of infected people. In addition, the virus can also be transmitted from infected mothers to newborns during childbirth or lactation.https://www.creative-biolabs...2020-11-0512 mincomHBV Vaccine - Creative BiolabsHepatitis B is an infectious liver disease that comes from hepatitis B virus infection. Acute infection may last for several weeks. If the virus stays in the body, it can cause severe chronic infections, leading to liver damage, liver cancer and death. But Hep B infection can be prevented. The current guidelines recommend high-risk populations for chronic HBV infection screening so that diagnosed individuals can carry out your proper hepatitis care. https://www.creative-biolabs.com/vaccine/hbv-vaccines.htm2020-11-0509 min
De Novo Antibody SequencingDe Novo Antibody Sequencing-8(1) The masses of the reduced light- and heavy-chain will be determined to validate the determined sequences. The light chain sequence must match within a +/- 1.2 Da range. For the heavy chain, +/- 1.8 Da is allowed. If the determined sequences do not match with the protein mass, you will not be charged. (Please note that this deviation is less than the weight of two protons).(2) Each mutation in the antibody will be validated by at least two meaningful spectra. This ensures that your sequence does not contain isobaric exchanges. Do not rely on peptide maps alone. [Isobaric exchanges are...2019-12-0200 minDe Novo Antibody SequencingDe Novo Antibody Sequencing-7Leucine can be distinguished from Isoleucine.W can be distinguished from GE, AD and SV (by mass accuracy).R can be distinguished from GV (by mass accuracy).Q can be distinguished from K (by mass accuracy).N can be distinguished from GG. Our DASS technology ensures bond breakage between GG. In addition, we use our proprietary derivatization technology for asparagine to ensure that it is not GG.Q can be distinguished from GA. Our DASS technology ensures bond breakage between GA. In addition, we use our proprietary derivatization technology for glutamine to ensure that is...2019-12-0200 minDe Novo Antibody SequencingDe Novo Antibody Sequencing-6IgMs can usually be sequenced like normal IgGs. Slightly more sample may be required. As the constant region is modified by several glycans, which cannot be cleaved by PNGase F, we can only guarantee complete coverage for the variable part of the antibody.Our method works with all antibody formats from all species (mouse, rat, hamster, llama, rabbit, etc.; Fab, scFv, etc.).Some mAbs are coupled to matrices. This is compatible with our sequencing method, but more samples may be required.Many older hybridomas may produce 2 light chains (one is the kappa-chain of myeloma MOPC 21). These mixtures...2019-12-0200 minDe Novo Antibody SequencingDe Novo Antibody Sequencing-5Sequencing of Fluorochrome mAbs, IgMs, and other Non-Standard AntibodiesWe have over 90% chance to get the sequence from tiny amount of your precious sample (e.g. 20 µg).We can enrich even very dilute sample (e.g. western blot antibody) for sequencing.Our method is usually not affected by ligands (FITC, Biotin, Alexa) coupled to antibodies. Larger protein ligands (PE, APC) make sequencing slightly more challenging. However, this can be compensated by using a higher protein concentration, because sequencing quality is dependent on sample amount.2019-12-0200 minDe Novo Antibody SequencingDe Novo Antibody Sequencing-4Our method generates many overlapping peptides during the fragmentation process, enabling us to sequence very long stretches of unknown amino acids. The high quality of MS/MS spectra in combination with intelligent data mining, allows us to read the CDR3 like a book. The technique is so powerful that we were able to sequence a 20 kDa protein, which had no homolog in the database. http://www.creative-biolabs.com/next-generation-antibody-sequencing.html2019-12-0200 minDe Novo Antibody SequencingDe Novo Antibody Sequencing-3De Novo Sequencing of the CDR3 RegionWhile the CDR3 of the light chain is mostly encoded by the germline sequences, the CDR3 of the heavy chain is usually not available in databases. It is encoded by the so-called D-segments, but these are modified by nucleases and terminal transferases. Typically, only 1-4 AA of a D-segment remains in the matured antibody. The rest of the D-segment is “artificial” and has to be sequenced de novo. http://www.creative-biolabs.com/next-generation-antibody-sequencing.html2019-12-0200 minDe Novo Antibody SequencingDe Novo Antibody Sequencing-1Due to the high number of peptides, we get sequence information for EVERY peptide bond in the antibody. Typically, 20-70 different MS/MS spectra are generated for each amino acid (AA) position. Hence, even the hardest sequences of proline and arginine-rich peptides can be resolved. As the order of all amino acids is clear, there is no need for time-consuming techniques like Edman sequencing. http://www.creative-biolabs.com/next-generation-antibody-sequencing.html2019-12-0200 minDe Novo Antibody SequencingDe Novo Antibody Sequencing-2One-Stop De Novo Antibody Sequencing ServicesSequencing of the V and J and C Segments by DASSThe V and J and C gene segments of antibodies are available in public databases. However, during the maturation of an antibody, the B cell introduces hypermutations into the sequence to optimize the affinity. Our mapping algorithm is error tolerant and can match the "mutated" peptides to the corresponding germline, reliably.2019-12-0200 min
com-baseShark AntibodyAs we know, sharks have been in existence for over 500 million years. From an evolutionary perspective of vies, they are among the oldest animals with a “modern” immune system not unlike that of humans. Shark blood contains large quantities of urea that makes their antibodies much more robust than that of other vertebrates. While urea protects sharks from dehydration, it can also destabilize sensitive protein molecules such as antibodies. https://www.creative-biolabs.com/blog/index.php/native-shark-antibody-discovery-used-in-shark-monoclonal-antibody-production/2019-08-1600 mincom-baseHybridoma TechnologyThe unique guinea pig hybridoma technology is adopted to generate monoclonal guinea pig antibodies. Through fusing with our specific myeloma as partner, guinea pig hybridoma can be developed with excellent stability and productivity. https://www.creative-biolabs.com/blog/index.php/creative-biolabs-makes-breakthrough-in-guinea-pig-monoclonal-antibody-production-with-hybridoma-technology/.2019-08-1601 mincom-baseGuinea Pig Monoclonal AntibodyGuinea pig, which is suggested as domesticated descendants of a closely related species of cavy, is a kind of rodent that does not exist naturally in the wild. It is one of the earliest species that has been applied in the field of research and investigation for more than 20 decades. However, as the genetic research of guinea pig (such as guinea pig antibody) has fallen behind during the early 20th century, it becomes less popular than mice and rats in a variety of research (such as humanized mice antibody). Even though, it is still an ideal source to generate antibodies...2019-08-1601 mincom-baseheavy-chain antibodiesThe first single domain antibody engineered from the heavy-chain antibodies and found in camelids has greatly stimulated the development of antibody market. Heavy-chain antibodies found in camelids can overcome or relieve immune system diseases. https://www.creative-biolabs.com/blog/index.php/single-domain-antibody-made-it-possible-for-curing-diseases-with-antibody-drugs/2019-08-1601 mincom-baseAntibody DrugsThe Rapid Development of Antibody DrugsExcept brain, the most sophisticated part in a human body is the immune system. In a sense, we are living in a world surrounded by various bacteria. They never cease disturbing us by taking over our living surroundings as their ideal place for breeding. Under this circumstance, antibody is the only one that can take the responsibility of an inner protector. Each antibody model has its own mission. When foreign objects, including microorganism, allergen and toxin, are trying to invade the immune system, antibodies will send out unique chemical signals on receiving...2019-08-1600 mincom-baseProtein PurificationReagents and apparatus of Protein Expression, Isolation and PurificationReagentsLB fluid medium: Tryptone 10g, yeast extract 5g, NaCl 10g.Mix all together with distilled water to 1000mL.Ampicillin: 100mg / mLSample: Buffer Solution. 100 mM NaH2PO4, 10 mMTris, 8M Urea, 10 mM2-ME, pH8.0Washing Buffer: 100 mM NaH2PO4, 10 mM Tris, 8 M Urea, pH6.3Elution Buffer: 100mM NaH2PO4, 10 mMTris, 8M Urea, 500 mM Imidazole, pH8.0IPTG https://www.creative-biolabs.com/blog/index.php/protein-expression-isolation-and-purification/2019-08-1600 mincom-baseProtein IsolationExperiment Principles of Protein Expression, Isolation and PurificationExpression of gene clone in cells is of great significance in both theoretical research and experimental applications. We can explore and study gene function and expression mechanism expression with gene expression. What’s more, the encoded protein by cloned gene expression can be used in researching the structure and function.E.coli (Escherichia coli ), whose exogenous expressing productivity is much higher than that of other gene expression systems, is currently the most widely used protein expression system. The number of target protein expressed may even exceed 80% of the to...2019-08-1600 mincom-baseProtein ExpressionProtein Expression, Isolation and PurificationLearn the approaches and meaning of the expression of cloned genes.Learn the method of recombinant protein affinity chromatography, isolation and purification2019-08-1601 min
IVDCMV diagnosticsCMV is a commonly known virus that can infect almost anyone. Once acquired, the body will retain the virus for life. CMV is usually harmless to organisms so most people are unaware of being infected. Sometimes it results in some long-term consequences in babies, such as hearing loss, visual deficits, or cognitive delays, if a woman catches it during pregnancy so-called congenital CMV. It is also known as herpesvirus type 5, a member of the herpes family that affects a host of individuals across the lifespan. It is often gained during late childhood or early adulthood with rarely symptomatic, except in...2019-08-1201 minIVDInflammatory markerThere are two different types of inflammation: acute and chronic (sometimes called systemic) inflammation. https://www.creative-biolabs.com/drug-discovery/diagnostics/ivd-antibodies-for-inflammatory-disease.htmWhile acute inflammation arises after a cut or scrape in the skin, an infected ingrown nail, a sprained ankle, a blow, a sore throat from a cold or flu, or exercise (especially intense training), which starts quickly and generally disappears in a few days. Examples of diseases and conditions with acute inflammation include:Acute bronchitisAcute appendicitisAcute dermatitisAcute tonsillitisAcute infective meningitisAcute sinusitisChronic...2019-08-1201 minIVDPancreatic Cancer markerWarning signs of pancreatic cancer include pain, weight loss, fatigue, and increased abdominal fluid. Patients complaining about these symptoms often get an evaluation including a physical examination, blood tests, X-rays, and an ultrasound. Computed tomography (CT scan) and endoscopic ultrasound (EUS) are used both to confirm the diagnosis and to help determine the resectability of the tumor. Magnetic resonance imaging (MRI) and positron emission tomography may also be used. If a pancreatic mass is observed, that raises the suspicion of pancreatic cancer and then a biopsy is performed to yield a diagnosis. Pancreatic Cancer marker plays crucial in the diagnosis...2019-08-1201 minIVDautoantibodyDiagnosing an autoimmune disease involves identifying the antibodies your body is producing:Autoantibody tests: any of several tests that look for specific antibodies to your own tissues.Antinuclear antibody tests: a type of autoantibody test that looks for antinuclear antibodies, which attack the nuclei of cells in your body.Complete blood count: measures the numbers of red and white cells in your blood; when your immune system is actively fighting something, these numbers will vary from the normal.C-reactive protein (CRP): elevated CRP is an indication of inflammation throughout your body erythrocyte sedimentation rate: this...2019-08-1201 minIVDAntibody DiscoveryIn Vitro Diagnostics (IVD) are tests used to detect and in diagnosis of disease, infections or other medical conditions, including a determination of the state of health, in order to cure, mitigate, treat, or prevent disease or its sequelae. They’re intended for use in the collection, preparation, and examination of specimens taken from the human body.Common uses for in vitro diagnostics including but not limited to disease screening, infections detecting, therapeutic monitoring, ensuring blood is safe to be used in transfusions, and recognizing of allergies and the measurement of hormones and other biological markers in body sp...2019-08-1200 minIVDtumor markerTumors can be benign or malignant. Tumors that stay in one spot and demonstrate limited growth are generally considered to be benign. Malignant cells are much “nimbler” than non-malignant ones. Malignant cells can pass more easily through smaller gaps, as well as applying a much greater force on their environment compared to other cells. Cells from malignant tumors can invade nearby tissues. They can also break away and spread to other parts of the body. Tumors can grow and interfere with the digestive, nervous, and circulatory system, and they can release hormones that alter body function. https://www.creative-biolabs.com/drug...2019-08-1200 minIVDThyroid Cancer testComposed of two lobes connected by an isthmus, the thyroid gland is an endocrine gland in the neck. It is located at the front of the neck. The main function of thyroid gland is to secrete thyroid hormones, which primarily influence the metabolic mechanism and protein synthesis. Thyroid cancer is cancer that develops from the tissues of the thyroid gland. It is a disease that involves the abnormality of cell growth. Potentially, they have the ability to spread to other parts of the body. Symptoms can include swelling or a lump in the neck. Tumors may originate from and spread...2019-08-1201 minIVDCancer markerCancer markerCancer is a class of diseases characterized by out-of-control cell growth. In all types of cancer, some of the body's cells begin to divide without stopping and spread into surrounding tissues. Normally, cells in the body follow an orderly path of growth, division, and death. Programmed cell death is called apoptosis, and when this process breaks down, cancer begins to form. Unlike regular cells, cancer cells do not experience programmatic death and instead continue to grow and divide. These extra cells can form a mass called a tumor. https://www.creative-biolabs.com/drug-discovery/diagnostics/cancer.htm2019-08-1202 min
antibodyanti egfr monoclonal antibodiesMouse Anti-EGFR Recombinant Antibody (IHC565)Provided herein is a Mouse monoclonal antibody against Epidermal Growth Factor Receptor. The antibody can be used for immunoassay techniques, such as IHC. And it gave a positive signal in Breast Carcinoma.https://www.antibody-creativebiolabs.com/search.aspx?key=egfr&ty=product&pmkid=0&fl=8,&flv=egfr~2019-08-1000 minantibodycd68 antibodyRabbit Anti-CD68 Recombinant Antibody (SP251)This product is a Rabbit antibody that recognizes CD68. The antibody SP251 can be used for immunoassay techniques such as: FC, IHC-P. https://www.antibody-creativebiolabs.com/search.aspx?key=cd68&ty=product&pmkid=0&fl=8,&flv=cd68~2019-08-1000 minantibodyanti cd68 antibodyMouse Anti-CD68 Recombinant Antibody (IHC068) Provided herein is a Mouse monoclonal antibody against CD68 Molecule. The antibody can be used for immunoassay techniques, such as IHC. And it gave a positive signal in Tonsil. https://www.antibody-creativebiolabs.com/search.aspx?key=cd31&ty=product&pmkid=0&fl=8,&flv=pecam1~2019-08-1000 minantibodycd44 antibodyMouse Anti-CD44 Recombinant Antibody (VFF-14)The product is antibody recognizes CD44. The antibody VFF-14 immunoassay techniques such as: WB. https://www.antibody-creativebiolabs.com/search.aspx?key=cd44&ty=product&pmkid=0&fl=8,&flv=cd44~2019-08-1000 minantibodyanti cd44 antibodyRabbit Anti-CD44 Recombinant Antibody (RM264) The product is antibody recognizes CD44. The antibody RM264 immunoassay techniques such as: WB, IHC. https://www.antibody-creativebiolabs.com/search.aspx?key=cd44&ty=product&pmkid=0&fl=8,&flv=cd44~2019-08-1000 minantibodyanti cd44Mouse Anti-CD44 Recombinant Antibody (1G4) The product is antibody recognizes CD44. The antibody 1G4 immunoassay techniques such as: WB, ELISA. https://www.antibody-creativebiolabs.com/search.aspx?key=cd44&ty=product&pmkid=0&fl=8,&flv=cd44~2019-08-1000 minantibodypecam antibodyRabbit Anti-PECAM1 Recombinant Antibody (RMCD31-1)This product is a rabbit antibody that recognizes PECAM1. The antibody RMCD31-1 can be used for immunoassay techniques such as: IHC, IF. https://www.antibody-creativebiolabs.com/search.aspx?key=cd31&ty=product&pmkid=0&fl=8,&flv=pecam1~2019-08-1000 minantibodypecam 1 antibodyRabbit Anti-PECAM1 Recombinant Antibody (RMCD31-1)This product is a rabbit antibody that recognizes PECAM1. The antibody RMCD31-1 can be used for immunoassay techniques such as: IHC, IF. https://www.antibody-creativebiolabs.com/search.aspx?key=cd31&ty=product&pmkid=0&fl=8,&flv=pecam1~2019-08-1000 mincomMembrane ProteinsWith extensive experience, we have successfully accomplished several challenging projects. Here we introduce our world-leading membrane protein production and antibody development services against those located on intracellular membranes by robust platforms and technologies. Details are elaborated at https://www.creative-biolabs.com/other-membrane-proteins-and-corresponding-antibody-development.html.2019-07-0401 mincomAntibody Service FAQStill obsessed with how to improve your antibody design projects?Antibody Service FAQ helps with your problem. Details can be found at https://www.creative-biolabs.com/antibody-service-faq.html.2019-07-0401 mincomSurface Technology FAQSurface Technology FAQ：What is the surface display technology?The question is exhaustively answered at https://www.creative-biolabs.com/surface-technology-faq.html.2019-07-0400 mincomAntibody&Antigen FAQAntibody&Antigen FAQs covering almost every aspect are provided for your reference. More info can be referred to at https://www.creative-biolabs.com/antibody-antigen-faq.htm.2019-07-0400 mincomPhage Display FAQCreative Biolabs has long-term devoted to the development and application of phage display technology. With years of experience, our scientists have developed several phage display based platforms and tailored hundreds of particular libraries and thousands of specific antibody products to boost our global customers’ research and project goals. All the Phage Display FAQs can be reached at https://www.creative-biolabs.com/phage-display-service.html.2019-07-0400 mincomhumanized antibodiesCreative Biolabs has extensive experience to offer the antibody humanization service for therapeutic and diagnostic development. We have successfully performed 15 mouse/rat humanization projects during the past decade with at least one humanized antibody entered the clinical trials. We also provide humanization service to antibodies derived from other species, such as non-human primate (NHP), rabbit, dog, chicken, llama and etc. Details can be found at https://www.creative-biolabs.com/antibody-humanization.html.2019-07-0201 mincomantibody optimization FAQFAQ relevant to antibody optimization is ready for your reference at https://www.creative-biolabs.com/faq.htm. Please visit the website to find if it helps.2019-07-0200 mincomantibody modification FAQFAQ regarding antibody can be reached at https://www.creative-biolabs.com/faq.htm to ensure that all your problems encountered be solved quickly.2019-07-0200 min
Antibody Categoriesanti tnfMore anti tnf antibody products can be found at https://www.antibody-creativebiolabs.com/search.aspx?key=anti-tnf&ty=product&pmkid=0&fl=8,&flv=tnf~.2019-06-1101 minAntibody Categoriesdsdna antibodyRecombinant dsdna antibody products are available at https://www.antibody-creativebiolabs.com/search.aspx?key=anti-dsdna&ty=product&pmkid=0&fl=8,&flv=dsdna~. This podcast is one of them. Please visit the website for more information.2019-06-1101 minAntibody Categoriesanti histone antibodyRecombinant anti histone antibody products are available at https://www.antibody-creativebiolabs.com/search.aspx?key=anti-p53&ty=product&pmkid=0&fl=8,&flv=p53~.2019-06-1101 minAntibody Categoriesanti his antibodyFeatures of anti his antibody can be found at https://www.antibody-creativebiolabs.com/search.aspx?key=his&ty=product&pmkid=0&fl=8,&flv=6-his~. Please visit for details.2019-06-1100 minAntibody Categoriesanti flag antibodyMore anti flag antibody can be found at https://www.antibody-creativebiolabs.com/search.aspx?key=flag&ty=product&pmkid=0&fl=8,&flv=flag~.2019-06-1100 minAntibody Categoriesanti dsdnaDetailed introduction of Mouse Anti-dsDNA Recombinant Antibody (DSD/958) can be viewed at https://www.antibody-creativebiolabs.com/search.aspx?key=anti-dsdna&ty=product&pmkid=0&fl=8,&flv=dsdna~.2019-06-1101 minAntibody Categoriesanti androgensMouse Anti-AR Recombinant Antibody (IHC511) Information Introduction. Please visit https://www.antibody-creativebiolabs.com/search.aspx?key=anti-ar&ty=product&pmkid=0&fl=8,&flv=ar~for printed version.2019-06-1101 minAntibody Categoriesactin antibodyInformation of Recombinant Mouse Anti-Actin Recombinant Antibody (2D7):https://www.antibody-creativebiolabs.com/search.aspx?key=actin&ty=product&pmkid=0&fl=8,&flv=actin~2019-06-1101 min