Shows
Designing The Best YOU!The 3Rs of Job Searching: Why Senior Executives Aren't Getting Hired (Results, Relevance & Resonance)If you're a senior professional or executive stuck in a long, frustrating job search, this episode explains exactly why and what to do instead. In today's market, experience alone isn't enough. Even highly qualified leaders are getting ghosted by recruiters, losing final-round interviews, or passed over for candidates with "less impressive" resumes. The problem isn't the economy. It's that most executives are missing one (or more) of the three hiring filters that actually determine who gets hired at senior levels. In this episode, we break down the 3Rs of Job Searching: Results: Why listing...
2025-12-3122 min
Sounds of ScienceBehind the Breakthroughs: Rethinking Animal Research with the 3RsWhat happens when cutting-edge science meets compassion? In this episode of Sounds of Science, host Mary Parker sits down with two pioneers reshaping the future of research: Elizabeth Nunamaker, Executive Director of Global Animal Welfare and Training at Charles River, and Dr. Megan LaFollette, Executive Director of the 3Rs Collaborative. From digital biomarkers to environmental health monitoring, they reveal how innovation and collaboration are redefining what’s possible in animal welfare — and raising the bar for ethical, high-quality research. Tune in to explore the tools, strategies, and bold ideas driving meaningful change across the scientific community.Show Note...
2025-06-0344 min
The Hair TherapistHaiR-3Rs & CPR: Transforming Salons into Lifelines In this episode, we are joined by Genevive Chadwick from CPR Hair to discuss their inspiring partnership with HaiR-3Rs, a program of FVREE, a leading specialist family violence service in Victoria. Genevive shares how CPR Hair is empowering salon professionals to recognise, respond, and refer clients experiencing domestic and family violence, turning salons into safe spaces for those in need. We explore the transformative impact of the HaiR-3Rs training program, which equips hairdressers and beaty therapists with the tools and confidence to support clients facing difficult situations. Genevive also highlights the importance of f...
2025-03-2621 min
On Tech Ethics with CITI Program3Rs Technologies in Animal Research - On Tech EthicsDiscusses the 3Rs of animal research, teaching, and testing (replacement, reduction, and refinement), including the methods and technologies that support their application. Our guest today is Megan LaFollette, who is the Executive Director at The 3Rs Collaborative, where she advances better science for both people and animals. She received her PhD and Master of Science in Animal Behavior & Welfare from Purdue University. She is an expert in advancing the implementation of practical, impactful, and evidence-based 3Rs techniques. Additional resources: The 3Rs Collaborative: https://3rc.org/ The 3Rs Collaborative Initiatives: https://3rc.org/works...
2025-01-0725 min
Modern Math Teacher- Teaching Strategies for High School and Middle School Math TeachersEp 30: The 3Rs of Student Engagement: Real, Relevant, Rigorous Math LessonsIn this engaging episode titled "The 3Rs of Engagement: Real, Relevant, Rigorous Math Lessons," we embark on a journey to unlock the secrets of extraordinary teaching. Inside, we dive deep into the importance of keeping math instruction: Real, Relevant, and Rigorous, providing actionable strategies and insights for educators aiming to elevate student engagement.Today's Topics - Importance of the 3Rs: Real, Relevant, and Rigorous elements as keys to unlocking profound student understanding.- Practical Implementation: Utilizing AI tools like teacher-tools.ai to transform word problems, and designing projects c...
2024-01-1605 min
Experts in SportE42: Encouraging children's healthy eating through the 3RsIn this episode of the “Experts in Sport” podcast, host Martin Foster sits down with Psychology Lecturer Dr Clare Holley, Research Associate Dr Lucy Porter, and Dan Parker, Chief Executive of VegPower. They discuss Dr Holley and Dr Porter’s research into child eating behaviors and temperament, as well as encouraging children to eat their vegetables, highlighting three methods known as “the 3Rs” – Role-modelling, Re-offering, and Rewarding. Timestamps: 00:00 – 01:08 Introduction & career background information on our guests 01:09 – 03:35 The 3Rs and their importance 03:36 – 18:55 Research findings on the 3Rs and child temperament 18:56 – 31:30 Obstacles parents may encounter + r...
2023-05-1047 min
PhorestFM: Building a Stronger Industry, TogetherBrianna Myors on Family Violence & Supporting Salon Professionals as Champions of Change (HaiR-3Rs)[265] Hair3Rs exists under the parent organisation FVREE, which supports Victim Survivors and works on breaking the cycle of family violence. Launched in 2018 as a pilot program for Melbourne salon professionals, the training was so successful that HaiR-3Rs is now available to salon professionals across Australia. On this week's episode, Program Manager Brianna Myors joins PhorestFM to discuss the program and how they support salon professionals to be champions of change.
Trigger Warning: This podcast contains discussions about sensitive topics that may be distressing to some listeners. These topics include but are not limited to family...
2023-04-2458 min
3 Minute 3RsAssessing enrichment, consolidating animal research guidance and understanding mouse aggressionFebruary 2023 The papers behind the pod: Hobbiesiefken U et al. (2023). Rating enrichment items by female group-housed laboratory mice in multiple binary choice tests using an RFID-based tracking system. PloS one 18(1): e0278709. doi: 10.1371/journal.pone.0278709Petkov C et al. (2022). Unified ethical principles and an animal research ‘Helsinki’ declaration as foundations for international collaboration. Current Research in Neurobiology 3:100060. doi: 10.1016/j.crneur.2022.100060Weber E et al. (2023). Aggression in Group-Housed Male Mice: A Systematic Review. Animals 13(1):143. doi: 10.3390/ani13010143It’s the third Thursday of February and you’re listening to the final episode of 3 minute 3...
2023-02-1604 min3 Minute 3RsReplacing animal-derived reagents, simulating in utero microinjections and clicker training for mouse gait assessmentJanuary 2023 The papers behind the pod: Cassotta M et al. (2022). A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation. Engineering in Life Sciences 22(9):561-604. doi: 10.1002/elsc.202100167 Nuber M et al. (2022). Development of a 3D simulator for training the mouse in utero electroporation. PLOS One 17(12): e0279004. doi: 10.1371/journal.pone.0279004Dickmann J et al. (2022). Clicker Training Mice for Improved Compliance in the Catwalk Test. Animals 12(24): 3545. doi: 10.3390/ani12243545Happy New Year to all 3 Minute 3Rs listeners. It’s 2023, and we’re back with three more papers highlighting efforts...
2023-01-1904 min3 Minute 3RsKidney organoid vascularisation, implementing masking and quantitatively assessing experiment severityDecember 2022The papers behind the pod: Menéndez ABC et al. (2022). Creating a kidney organoid-vasculature interaction model using a novel organ-on-chip system. Scientific Reports 12:20699. doi: 10.1038/s41598-022-24945-5Karp N et al. (2022). A qualitative study of the barriers to using blinding in in vivo experiments and suggestions for improvement. PLOS Biology 20(11): e3001873. doi: 10.1371/journal.pbio.3001873Talbot S et al. (2022). RELSA—A multidimensional procedure for the comparative assessment of well-being and the quantitative determination of severity in experimental procedures. Frontiers in Veterinary Science 9:937711. doi: 10.3389/fvets.2022.937711 It’s the third Thursday of December, and...
2022-12-1504 min3 Minute 3RsAssessing pain, living systematic reviews and inducing focal hypoxia in human neuronsNovember 2022The papers behind the pod: Aulehner K et al. (2022). Grimace scale, burrowing, and nest building for the assessment of post-surgical pain in mice and rats—A systematic review. Frontiers in Veterinary Science 9:930005. doi: 10.3389/fvets.2022.930005Hair K et al. (2022). ‘Living’ evidence frameworks for in vivo animal research: towards translational evidence-based medicine. BMJ Evidence-Based Medicine 2022;27:A17. doi: 10.1136/ebm-2022-EBMLive.31 Wong J et al. (2022). Electrochemically induced in vitro focal hypoxia in human neurons. Frontiers in Cell and Developmental Biology 10:968341. doi: 10.3389/fcell.2022.968341It’s the third Thursday of November, and you’re listening to 3 Minute 3Rs...
2022-11-1704 min3 Minute 3RsStatistical planning, human cell cultures for toxoplasma and preventing boredom in laboratory rodentsOctober 2022The papers behind the pod: Piper, SK et al. (2022). Statistical review of animal trials—A guideline. Biometrical Journal https://doi.org/10.1002/bimj.202200061 Gargaté MJ et al. (2022). Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model. Pathogens https://doi.org/10.3390/pathogens11091038 Mieske P et al. et al. (2022). Bored at home?—A systematic review on the effect of environmental enrichment on the welfare of laboratory rats and mice. Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2022.899219 It’s the third T...
2022-10-2004 min3 Minute 3RsLarger species refinement special: improving the welfare of rabbits, non-human primates and sheepSeptember 2022The papers behind the pod: Pinho RH et al. (2022). Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus). PLoS ONE 17(5): e0268973. https://doi.org/10.1371/journal.pone.0268973 Stull C, Heagerty A and Coleman K (2022). Video Conference Technology as a Tool for Pair Introduction in Rhesus Macaques. Animals 12(14): e1783. https://doi.org/10.3390/ani12141783 Zentrich E et al. (2022). Postoperative Severity Assessment in Sheep. European Surgical Research, in press. https://doi.org/10.1159/000526058 It’s the third Thursday of September, and you’re listening to 3 Mi...
2022-09-1504 min3 Minute 3RsBetter behavioural research, imaging with microbots and how housing density affects mouse microbiomesAugust 2022The papers behind the pod: Arjmand S et al. (2022). Tips and traps for behavioural animal experimentation. Acta Neuropsychiatrica, in press. https://doi.org/10.1017/neu.2022.4Wrede P et al. (2022). Real-time 3D optoacoustic tracking of cell-sized magnetic microrobots circulating in the mouse brain vasculature. Science Advances 8(19). https://doi.org/10.1126/sciadv.abm9132 Russell A et al. (2022). Reduced housing density improves statistical power of murine gut microbiota studies. Cell Reports 39(6): e110783. https://doi.org/10.1016/j.celrep.2022.110783It’s the third Thursday of August, and you’re listening to 3 Minute 3Rs, your monthly recap of eff...
2022-08-1804 min3 Minute 3Rs3Rs Prize: A benchtop organ-on-a-chip fabrication method and an ex vivo model of focal demyelinationJuly 2022The papers behind the pod: Ferreira DA et al. (2021). Alternative to Soft Lithography for the Fabrication of Organ-on-a-Chip Elastomeric-Based Devices and Microactuators. Advanced Science 8:2003273. https://doi.org/10.1002/advs.202003273Eigel D et al. (2019). Cryogel scaffolds for regionally constrained delivery of lysophosphatidylcholine to central nervous system slice cultures: A model of focal demyelination for multiple sclerosis research. Acta Biomaterialia 97:216. https://doi.org/10.1016/j.actbio.2019.08.030It’s the third Thursday of July, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of anima...
2022-07-2104 min
Resilience in LeadershipEp 4 - 3Rs: Resilience for Recovery and Rebuild (Part 1)Dean Pax (PhD) specializes in strategic planning, management, local & regional business development, enterprise start-up, growth, financial wellness, and family business expansion. He is the Dean of Entrepreneurship/Platinum Circle of Bo Sanchez’s Truly Rich Club and a lead advocate and AMEN Pioneer mentor of Go Negosyo. He is also a former adjunct faculty on Entrepreneurship at the Asian Institute of Management (AIM).
At present, he is the Chairman of SPAC Information Technology Inc., a company that provides IT business solutions and systems developers augmentation in the US. He is also a director of the CARAGA Renewable Ene...
2022-07-1551 min3 Minute 3RsHumane intervention points, virtual gene knockout and ex vivo brain slices for Parkinson's researchJune 2022The papers behind the pod: Williams WO and Baneux P (2022). Humane Intervention Points: Refining endpoint terminology to incorporate non-euthanasia intervention options to improve animal welfare and preserve experimental outcomes. Laboratory Animals, in press. https://doi.org/10.1177/00236772221090801 Osorio D et al. (2022). scTenifoldKnk: An efficient virtual knockout tool for gene function predictions via single-cell gene regulatory network perturbation. Patterns 3(3): e100434. https://doi.org/10.1016/j.patter.2022.100434Moudio S et al. (2022). Exposure of α-Synuclein Aggregates to Organotypic Slice Cultures Recapitulates Key Molecular Features of Parkinson's Disease. Frontiers in Neurology 13: e826102. https://doi.org/10.3389/fneur.2022.826102 It’s the t...
2022-06-1604 min3 Minute 3RsMicrobrains for neurotoxicity testing, improved experimental design, and post-op severity assessmentMay 2022 The papers behind the pod: Wang, Q et al. (2022). Assessment of a 3D neural spheroid model to detect pharmaceutical-induced neurotoxicity. ALTEX 39. https://doi.org/10.14573/altex.2112221 Reynolds P.S. (2022) Between two stools: preclinical research, reproducibility, and statistical design of experiments. BMC Res. Notes 15, 73. https://doi.org/10.1186/s13104-022-05965-w Tappe-Theodor, A. et al. (2022) The “WWHow” Concept for Prospective Categorization of Post-operative Severity Assessment in Mice and Rats. Front. Vet. Sci. 9, 841431. https://doi.org/10.3389/fvets.2022.841431It’s the third Thursday of May, and you’re listening to 3 Minute 3Rs, your monthly re...
2022-05-1904 min
StudentCast on 3Rs - Tierschutz trifft Wissenschaft.Organoids and brain research : Dr. Jan BruderWithin this episode Dr. Jan Bruder from the Max Planck Institute in Münster gives answers to our questions about organoids, starting with “What are organoids anyway?”. He gives insight into the procedure of the automated production of organoids and describes their advantages. He explains new testing methods, which are for example used in the research of Parkinson's disease. Also ethical questions concerning mini brains are addressed.
2022-05-1026 min
Learn MarketingS4C3A6 What are the 3Rs of Influence?Season/Course 4: Marketing Channels & Communication
Chapter 3: Media
Abstract 6
What are the 3Rs of Influence? The 3 Rs of Influence: Reach, Resonance, and Relevance. Getting the key players who have the power to influence purchasing decisions for your company on your side can supercharge awareness and lead generation, but the effort you put into building relationships with them is only as impactful as the effect those influencers have on their communities. Let's understand this marketing concept in more detail in this episode.
For more awesome learning about marketing visit learn.admybrand.com for booking ads...
2022-04-2103 min3 Minute 3RsHow environment and experimenter affect reproducibility, ex vivo adipocytes, and rodent enrichmentApril 2022The papers behind the pod: Nigri M et al. (2022). Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice. Frontiers in Behavioural Neuroscience 16: e835444. https://doi.org/10.3389/fnbeh.2022.835444 Vámos A et al. (2022) Mitophagy Mediates the Beige to White Transition of Human Primary Subcutaneous Adipocytes Ex Vivo. Pharmaceuticals 15(3): 363. https://doi.org/10.3390/ph15030363 Ratuski AS and Weary DM (2022) Environmental Enrichment for Rats and Mice Housed in Laboratories: A Metareview. Animals 12(4): 414. https://doi.org/10.3390/ani12040414 It’s the third Thursday of April, and you’re listening to 3 Minute 3Rs, your monthly recap o...
2022-04-2104 min3 Minute 3RsReplacement methods for drug development, toxicology testing and cardiovascular researchThe papers behind the pod: 1. Baran SW et al. (2022). Perspectives on the Evaluation and Adoption of Complex In Vitro Models in Drug Development: Workshop with the FDA and the Pharmaceutical Industry (IQ MPS Affiliate). ALTEX, in press. https://doi.org/10.14573/altex.21122032. Borba JVB et al. (2022) STopTox: An in Silico Alternative to Animal Testing for Acute Systemic and Topical Toxicity. Environmental Health Perspectives 130(2). https://doi.org/10.1289/EHP93413. van der Velden J et al. (2022) Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC working group on m...
2022-03-1704 min3 Minute 3RsIssues with conventional rodent housing, playpens for rats, and using sleep to assess welfareFebruary https://doi.org/10.1186/s12915-021-01184-0https://doi.org/10.1177/00236772211065920https://doi.org/10.1038/s41598-021-04502-2It’s the 3rd Thursday of February, and you’re listening to 3 Minute 3Rs. This month, we’re bringing you 3 refinement papers. Let’s start with rodent housing.Research rodents are conventionally housed in shoebox sized cages that limit their ability to perform natural behaviors such as nesting and burrowing. These restrictions are known to impair welfare, but could they even increase disease risk and shorten lifespans? A new meta-ana...
2022-02-1704 min3 Minute 3RsReproducibility in cancer biology, training rats for refined fMRI, and playpens for miceThe papers behind the pod:1. https://doi.org/10.7554/eLife.71601 & https://doi.org/10.7554/eLife.679952. https://doi.org/10.3389/fnins.2021.8056793. https://doi.org/10.1038/s41598-021-98356-3It’s the 3rd Thursday of January – happy new year! You’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. Of course, we focus on those three Rs, but many have suggested adding a fourth R to the list: reproducibility. Designing experiments with reproducibility in mind is a key aspect of reducing unnecessary animal use, a...
2022-01-2004 min
3 Minute 3RsDiscover the winners of this year's Global AAALAC/IQ AwardsThe papers behind the pod:1. A non-human primate in vitro functional assay for the early evaluation of TB vaccine candidates. npj Vaccines, January 2021. https://doi.org/10.1038/s41541-020-00263-72. Inducible dominant negative ErbB2 rat spermatogonial line for generation of transgenic rat model and dissecting ERBB2 tyrosine kinase mediated pathways. Experimental Oncology, June 2019. https://doi.org/10.32471/exp-oncology.2312-8852.vol-41-no-2.130263. Characterization of drug responses of mini patient-derived xenografts in mice for predicting cancer patient clinical therapeutic response. Cancer Communications, September 2018. https://doi.org/10.1186/s40880-018-0329-5Visit th...
2021-11-1805 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Thema Haut und OECD-Richtlinien : PD Dr. Robert Landsiedel - Topic skin and OECD guidelines : PD Dr. Robert LandsiedelIn dieser Folge erzählt Dr. Landsiedel über die Etablierung von Alternativmethoden und geht dabei beispielhaft auf die Testung der Hautsensibilisierung ein. Auch die OECD-Richtlinien werden in diesem Zusammenhang thematisiert und deren Entwicklungsprozess anhand des Beispiels der Richtlinie 497 diskutiert. – In this episode Dr. Landsiedel talks about the establishment of Non-Animal-Methods and goes as an example into testing of skin sensitization. Also the OECD guidelines are discussed in this context and the process of their development is exemplified on the basis of guideline 497.
2021-10-2753 min
3 Minute 3RsPeople and strategies behind 3Rs science: compassion fatigue, culture of care and systematic reviewsThe papers behind the pod:1. Professional Quality of Life in Research Involving Laboratory Animals. Animals 11(9), 2639 (2021) https://doi.org/10.3390/ani110926392. Improving culture of care through maximising learning from observations and events: Addressing what is at fault. Laboratory Animals (2021). https://doi.org/10.1177%2F002367722110371773. Epidemiology and reporting characteristics of preclinical systematic reviews. PLOS Biology (2021). https://doi.org/10.1371/journal.pbio.3001177Transcript:It’s the 3rd Thursday of October, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. This mont...
2021-10-2104 min
3 Minute 3Rs3 Minute 3Rs September 2021Sept 2021 You’re listening to the September episode of 3 Minute 3Rs. The papers behind the pod: Performance of preclinical models in predicting drug-induced liver injury in humans: a systematic review. Scientific Reports 11, 6403 (2021) https://doi.org/10.1038/s41598-021-85708-2 Ratlas-LH: An MRI template of the Lister hooded rat brain with stereotaxic coordinates for neurosurgical implantations Brain and Neuroscience Advances (2021) https://doi.org/10.1177/23982128211036332 O mouse, where art thou? The Mouse Position Surveillance System (MoPSS)—an RFID-based tracking system. Behavior Research Methods (2021) https://doi.org/10.3758/s13428-021-01593-7 [Transcript] It’s the 3rd Thursda...
2021-09-1603 min
3 Minute 3Rs3 Minute 3Rs August 2021You’re listening to the August episode of 3 Minute 3Rs!The papers behind the pod: Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. Heliyon 7, E07500 (2021) https://doi.org/10.1016/j.heliyon.2021.e07500 Strategies to encourage the adoption of social housing during cardiovascular telemetry recordings in non-rodents. Journal of Pharmalogical & Toxicological Methods 108, 106969 (2021). https://doi.org/10.1016/j.vascn.2021.106959 Refine acquisition of high-resolution chest CTs in macaques by free breathing. Laboratory Animals https://doi.org/10.1177%2F00236772211026562 Transcript:It’s the 3rd Thursday of August...
2021-08-1903 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Thema Bioprinting : Prof. Dr. Jens Kurreck - Topic bioprinting : Prof. Dr. Jens KurreckIn der Fortsetzung der Spezialfolge mit Herrn Prof. Dr. Kurreck wird nochmal detaillierter auf die Vielfältigkeit des 3D-Bioprintings eingegangen. Es werden Zukunftsfragen gestellt, technische Herausforderungen angesprochen und nach dem Nutzen des 3D-Bioprintings mit Hinblick auf das SARS-CoV-2-Virus gefragt. Die Zuhörer*innen dürfen sich auf spannende Fragen und lehrreiche Antworten freuen. - In the continuation of the special episode with Professor Kurreck, the diversity of 3D bioprinting is discussed in more detail. Future questions will be asked technical challenges will be addressed and the benefits of 3D bioprinting with regard to the SARS-CoV-2 virus will be discussed. The...
2021-08-1716 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Thema Bioprinting : Prof. Dr. Jens Kurreck - Topic bioprinting : Prof. Dr. Jens KurreckIn der 4. und 5. Episode geht es um das Thema 3D Bioprinting. Was kann man sich unter 3D-Druck von Zellen vorstellen? Was sind verwendete Materialien und welchen Beitrag kann dieser junge Ansatz zu den 3Rs liefern? Diesen und vielen weiteren Fragen hat sich Herr Prof. Dr. Kurreck von der TU Berlin in unserer Spezialfolge gestellt. - The 4th and 5th episode are about 3D bioprinting. What do we mean by 3D printing of cells? What materials are used and what contribution can this young approach make to the 3Rs? Prof. Dr. Kurreck from the TU Berlin answered these and many other...
2021-08-1620 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Austausch über spezifische Modelle : Prof. Dr. Horst SpielmannSehr oft ist die Testung von Substanzen am Tier nicht oder nur bedingt vorhersagekräftig für die Situation am Menschen. Dies wird sehr deutlich bei der Entwicklung neuer Arzneistoffe, die sich oft in den sog. klinischen Studien (Untersuchungen am Menschen) als nicht wirksam erweisen und/oder Nebenwirkungen aufweisen, obwohl sie vorher im Tierversuch getestet wurden. In dieser Podcast-Folge geht es um die Möglichkeiten, für die Entwicklung von Arzneistoffen auch tierversuchsfreie Methoden zu nutzen. Es werden auch einige Krankheiten und sogenannte Krankheitsmodelle vorgestellt und deren Vorteile bzw. die Schwierigkeiten bei deren Entwicklung erklärt.
2021-08-1536 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Talk about certain models : Prof. Dr. Horst SpielmannVery often substance testing on animals is not or just quite limited able to predict the human situation. This becomes very apparent in the development of new drugs, which often in so-called clinical studies (research on the human) turn out as effectless and/or show side effects, though they were tested on animals before. This podcast episode deals with the possibilities of using animal-free methods for drug development. Some diseases and so-called disease models are also presented and their benefits and the challenges of their development are explained.
2021-08-1436 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Diskussion über Validierung und Entwicklung von Alternativmethoden: Prof. Dr. Horst SpielmannIm zweiten Interview sprechen die drei Moderatorinnen mit Prof. Dr. Horst Spielmann über den Begriff "Validierung" und die Zentren für die Entwicklung von Ersatz- und Ergänzungsmethoden zu Tierversuchen. Anhand der in vitro Testung auf Phototoxizität wird eine Methode besprochen, die tierversuchsfrei die Vorhersage für die Situation am Menschen besser trifft als der Tierversuch. Auch die Vorteile von sog. OECD-Richtlinien für die Testung von Chemikalien werden angesprochen.
2021-08-1329 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Discussion about validation and development of alternative research methods : Prof. Dr. Horst SpielmannIn the second interview the three moderators talk together with Prof. Dr. Horst Spielmann about the term 'validation' and the centers for development of replacement and supplemental methods for animal experiments. Using the example of in vitro phototoxicity testing a method is discussed, which without animals predicts the human situation better than the animal test. Also the benefits of the so called OECD Guidelines for chemical testing are mentioned.
2021-08-1233 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Einführung in das 3R-Prinzip und den aktuellen Stand: Prof. Dr. Horst SpielmannIm ersten Interview mit Prof. Dr. Horst Spielmann klären die drei Moderatorinnen unter anderem die Fragen, wofür der Begriff "3R" steht und in welchen Bereichen Non-Animal-Methods (Methoden ohne Tiere, abgekürzt "NAM") schon genutzt werden. Dazu zählen z.B. die Testung auf Hautreizung und Augenreizung.
2021-08-1113 minStudentCast on 3Rs - Tierschutz trifft Wissenschaft.Introduction to the 3Rs principle and to the current status : Prof. Dr. Horst SpielmannIn the first interview with Prof. Dr. Horst Spielmann the three moderators enter in the question, what the phrase "3Rs" stands for and where non-animal methods (abb. 'NAM') are already established. This includes e.g. tests for skin irritation and eye irritation
2021-08-1017 min
Point of ConvergenceDuane West of 3Rs ConstructionToday I had the pleasure of talking with Duane West of 3Rs Construction . In this episode, Guy shares his play book of running a million dollar remodeling company and his secrets to leadership. Enjoy!
3Rs Construction Website:
https://www.3rsconstruction.com/
2021-07-1933 min
Autonomy HealthThe 3Rs When Cramps Hit YouThis episode is also available as a blog post: https://39autonomy.com/2021/07/18/the-3rs-when-cramps-hit-you/
2021-07-1801 min
3 Minute 3Rs3 Minute 3Rs July 2021It’s the 3rd Thursday of July and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. The papers behind the pod: SARS-CoV-2 infects an upper airway model derived from induced pluripotent stem cells. Stem Cells https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.1002/stem.3422A break from the pups: The effects of loft access on the welfare of lactating laboratory rats. PLos One https://doi.org/10.1371/journal.pone.0253020 3D printed rodent skin-skull-brain model: A novel animal-free approach for neurosurgical training. PLoS One https...
2021-07-1504 min
3 Minute 3Rs3 Minute 3Rs June 2021You’re listening to the June 2021 episode of 3 Minute 3Rs The papers behind the pod: Winning paper: Pellegrini L et al. (2020). Human CNS barrier-forming organoids with cerebrospinal fluid production. Science 369(6500): eaaz5626. doi.org/10.1126/science.aaz5626. Highly commended: Ashworth J et al. (2020). Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro. Matrix Biology 85-86: 15-33. doi.org/10.1016/j.matbio.2019.06.009. [Transcript]It’s the 3rd Thursday of June, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace...
2021-06-1703 min
Your Story is Our StoryThe New 3Rs Daughters Series - Part 3Welcome to the third episode of The New 3Rs Daughters Series, in the Your Story is Our Story Podcast. With your podcast host, Mr. Neil Foote.
Three Black Daughters, across generations, tell their stories of confronting and enduring racial prejudices in their school days. In this episode, the three daughters talk about race, culture and life in America
Mr. Dannie Gore gives us the Black History Lesson of the month.
A new episode of "The New 3Rs Daughters Series" comes out the first Thursday of every month at 1 PM Eastern Standard Time. ...
2021-06-0327 min
Comeback MinimalistS2 E18: Thrifting in India & 3Rs of Slow Fashion ft. Khyati ShahOur 17th guest in the #RealLifeIndianMinimalists series is Khyati Shah, an Ecopreneur.We talked about- Future of Thrifting in India and 3Rs of slow fashion.List of some Indian thrift stores-Thread.up_Thrift BananaWhat we woreThrift Mumbaihuckleberry hangersthe fine endscopper boom vintage Khyati's Instagram handle: forever_eco Request you to support the show in whatever way you can:
2021-06-0214 min
3 Minute 3Rs3 Minute 3Rs May 2021You’re listening to the April episode of 3 Minute 3Rs.The papers behind the pod:1. PCR Testing of Media Placed in Soiled Bedding as a Method for Mouse Colony Health Surveillance. JAALAS https://doi.org/10.30802/AALAS-JAALAS-20-0000962. New mosquito repellency bioassay for evaluation of repellents and pyrethroids using an attractive blood-feeding device. Parasites & Vectors https://doi.org/10.1186/s13071-021-04656-y3. Lung cancer organoids analysed on microwell arrays predict drug responses within a week. Nature Communications https://doi.org/10.1038/s41467-021-22676-1 Transcript: It’s the 3rd Thursday of May...
2021-05-2003 minYour Story is Our StoryThe New 3Rs Daughters Series - Part 2Welcome to the second episode of The New 3rs Daughters Series, in the Your Story is Our Story Podcast, with your podcast host, Mr. Neil Foote.
Three Black Daughters, across generations, tell their stories of confronting and enduring racial prejudices in their school days.
In this episode of the Your Story is Our Story Podcast, we bring you part two of our intergenerational conversations, featuring Lola West who is founding partner of Westfuller Advisors, Llc that provides strategic insight and investment advice to nationally recognized organizations and high net worth and exclusive clientele in...
2021-05-1325 min
3 Minute 3Rs3 Minute 3Rs April 2021You’re listening to the April episode of 3 Minute 3Rs.The papers behind the pod:1. Identification of individual zebrafish (Danio rerio): a refined protocol for VIE tagging whist considering animal welfare and the principles of the 3RS. Animals 11, 616 (2021) https://doi.org/10.3390/ani110306162. Enrichment for laboratory zebrafish – a review of the evidence and challenges. Animals 11, 698 (2021) https://doi.org/10.3390/ani110306983. Research relevant background lesions and conditions: ferrets, dogs, swine, sheep, and goats. ILAR J ilab005 (2021) https://doi.org/10.1093/ilar/ilab0054. *Bonus* Research-relevant background lesions and conditions in common avian and aquatic species. ILAR J ilab00...
2021-04-1503 min
3 Minute 3Rs3 Minute 3Rs March 2021You’re listening to the March episode of 3 Minute 3Rs.The papers behind the pod:1. Novel three-dimensional biochip pulmonary sarcoidosis model. PLoS One https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245805 2. A new group housing approach for non-human primate metabolism studies. Journal of Pharmacological and Toxicological Methods https://doi.org/10.1016/j.vascn.2020.1069473. The contribution of environmental enrichment to phenotypic variation in mice and rats. eNeuro https://doi.org/10.1523/ENEURO.0539-20.2021Transcript: It’s the 3rd Thursday of March and you’re listening to 3 Minute 3Rs, your monthly recap of ef...
2021-03-1804 min
3 Minute 3RsFebruary 2021You’re listening to the February episode of 3 Minute 3Rs.The papers behind the pod:1. In Vitro Tests for Assessing the Neutralizing Ability of Snake Antivenoms: Toward the 3Rs Principles. Frontiers in Immunology https://doi.org/10.3389/fimmu.2020.6174292. The Impact of Acute Loud Noise on the Behavior of Laboratory Birds. Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2020.6076323. A multicomponent screen for feeding behavior and nutritional status in Drosophila to interrogate mammalian appetite-related genes. Molecular Metabolism https://doi.org/10.1016/j.molmet.2020.101127Transcript: It’s the 3rd Thursday of February and you’re...
2021-02-1804 min
Within Normal Limits: Navigating Medical RisksAddressing Adverse Outcomes Part I: COPIC’s 3Rs and CANDOR ProgramsDr. Jeff Varnell, a surgeon and COPIC physician risk manager, is the guest for this episode that focuses on addressing adverse outcomes through COPIC’s 3Rs and CANDOR Programs. Dr. Varnell has managed the 3Rs (Recognize, Respond, and Resolve) process and highlights its origins and goals of maintaining the physician-patient relationship and improving the quality of medical care. He also talks about how 3Rs and CANDOR try to move away from an adversarial approach to one that guides physicians through managing difficult patient conversations and using best practices for disclosure that includes open and honest conversations. In addition, Dr. Varn...
2021-01-2715 min
3 Minute 3Rs3 Minute 3Rs January 2021You’re listening to the January episode of 3 Minute 3Rs.The papers behind the pod:1. Internal consistency and compatibility of the 3Rs and 3Vs principles for project evaluation of animal research. Laboratory Animals https://doi.org/10.1177/00236772209685832. Noise and Vibration in the Vivarium: Recommendations for Developing a Measurement Plan. JAALAS https://doi.org/10.30802/AALAS-JAALAS-19-0001313. A human tissue screen identifies a regulator of ER secretion as a brain-size determinant. Science https://doi.org/10.1126/science.abb5390 Transcript: It’s the 3rd Thursday of January and you’re listening to the first 3 Minute 3R...
2021-01-2104 min
3 Minute 3Rs3 Minute 3Rs December 2020You’re listening to the December episode of 3 Minute 3Rs.The papers behind the pod:1. Protective cranial implant caps for macaques. Journal of Neuroscience Methods https://doi.org/10.1016/j.jneumeth.2020.1089922. The ‘Cage Climber’ – A new enrichment for use in large-dimensioned mouse facilities. Applied Animal Behaviour Science https://doi.org/10.1016/j.applanim.2020.1050783. Skin swabbing is a refined technique to collect DNA from model fish species. Scientific Reports https://www.nature.com/articles/s41598-020-75304-1Transcript: It’s the 3rd Thursday of December and you’re listening to 3 Minute 3Rs...
2020-12-1703 min
VO’NELVO#032 The 3RsYou must do the 3Rs before the end of the year. Start 2021 off right with vision and insight.
2020-12-1304 min
2020-12-0304 min
3 Minute 3RsNovember 2020You’re listening to the November episode of 3 Minute 3Rs.The papers behind the pod:1. Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. Cell Stem Cell https://doi.org/10.1016/j.stem.2020.10.0042. Improving reproducibility in animal research by splitting the study population into several ‘mini-experiments’. Sci Rep https://doi.org/10.1038/s41598-020-73503-43. Three Pillars of Automated Home-Cage Phenotyping of Mice: Novel Findings, Refinement, and Reproducibility Based on Literature and Experience. Front Behav Neurosci https://doi.org/10.3389/fnbeh.2020.575434Transcript: It’s the 3rd Thursday of November and you’...
2020-11-1904 min
El Cafecito Constructivo11. 3RS: Primera certificación sostenible en República DominicanaCon mucho honor y orgullo, tenemos de invitados a Sherly Almonte y Marbellis Mirabal representantes de Sosteniblidad 3RS, empresa pionera en crear pautas sostenibles en el ámbito de diseño y construcción. Han creado la primera certificación sostenible en República Dominicana y en este episodio nos describen en detalle los criterios que han utilizado para crear esta certificación "aplatanada" y novedosa, al igual que las normativas y leyes que aplican. Sin falta analizamos las tipologías de proyectos a que aplica al igual que los costos que lo acompañan.Para más información y apoyo...
2020-10-1929 min
3 Minute 3Rs3 Minute 3Rs October 2020You’re listening to the October episode of 3 Minute 3Rs.The papers behind the pod:1. The epidemiology of fighting in group-housed laboratory mice. Scientific Reports https://www.nature.com/articles/s41598-020-73620-02. Development of a miniaturized 96-Transwell air–liquid interface human small airway epithelial model. Scientific Reports https://www.nature.com/articles/s41598-020-69948-23. Publication rate in preclinical research: a plea for preregistration. BMJ Open Science https://openscience.bmj.com/content/4/1/e100051Transcript: It’s the 3rd Thursday of October and you’re listening to 3 Minute 3R...
2020-10-1504 min
3 Minute 3Rs3 Minute 3Rs September 2020You’re listening to the September episode of 3 Minute 3Rs.The papers behind the pod:1. Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction. Scientific Reports https://www.nature.com/articles/s41598-020-66487-82. Automated and rapid self-report of nociception in transgenic mice. Scientific Reports https://www.nature.com/articles/s41598-020-70028-83. Zebrafish as an alternative animal model in human and animal vaccination research Laboratory Animal Research https://labanimres.biomedcentral.com/articles/10.1186/s42826-020-00042-4 Transcript: It’s the 3rd Thursda...
2020-09-1704 min
TrenchCastTrenchCast 02 - 3Rs - Resiliência, Recuperação e RenovaçãoA Covid-19 transformou o modus operandi de muitas empresas. E agora? Neste episódio, Simone Dias Musa, membro do Comitê Administrativo e sócia da área Tributária de Trench Rossi, fala sobre o modelo 3Rs: Resiliência, Recuperação e Renovação, que está ajudando as empresas a navegarem pelos impactos legais e comerciais a partir do momento que estamos vivendo.
2020-09-1016 min
3 Minute 3Rs3 Minute 3Rs August 2020You’re listening to the August episode of 3 Minute 3Rs.The papers behind the pod:1. The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. PloS Biology https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.30004102. Acute and chronic stress prevents responses to pain in zebrafish: evidence for stress-induced analgesia. Journal of Experimental Biology. https://jeb.biologists.org/content/223/14/jeb2245273. 3Rs-based optimization of mice behavioral testing: The habituation/dishabituation olfactory test. Journal of Neuroscience Methods. https://doi.org/10.1016/j.jneumeth.2019.108550 Transcript:It’s the 3rd Thursday of August, and...
2020-08-2004 min
3 Minute 3Rs3 Minute 3Rs July 2020You’re listening to the July episode of 3 Minute 3Rs.The papers behind the pod:1. Reproducibility of animal research in light of biological variation. Nature review Neuroscience https://www.nature.com/articles/s41583-020-0313-32. Inactivated rabies vaccines: Standardization of an in vitro assay for residual viable virus detection. Plos Neglected Tropical Diseases https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.00081423. A novel biosafety level 2 compliant tuberculosis infection model using a ΔleuDΔpanCD double auxotroph of Mycobacterium tuberculosis H37Rv and Galleria mellonella. Virulence...
2020-07-1604 min
3 Minute 3Rs3 Minute 3Rs June 2020You’re listening to the June episode of 3 Minute 3Rs.The papers behind the pod:A novel, high-welfare methodology for evaluating poultry red mite interventions in vivo. https://www.sciencedirect.com/science/article/pii/S030440171930041XPluripotent state transitions coordinate morphogenesis in mouse and human embryos. https://www.nature.com/articles/nature24675TranscriptIt’s the Third Thursday of June and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. Instead of the usual...
2020-06-1803 min
3 Minute 3Rs3 Minute 3Rs May 2020You’re listening to the May episode of 3 Minute 3Rs. The papers behind the pod: 1 Refining rodent models of spinal cord injury: https://doi.org/10.1016/j.expneurol.2020.1132732 Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders https://www.sciencedirect.com/science/article/pii/S08891591203025433 Identifying obstacles preventing the uptake of tunnel handling methods for laboratory mice: An international thematic survey https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231454TranscriptIt’s the Third Thursday of May and you’re...
2020-05-2103 min
3 Minute 3Rs3 Minute 3Rs April 2020You’re listening to the April episode of 3 Minute 3Rs, brought to you by the NC3Rs, North American 3Rs Collaborative, and Lab Animal. The papers behind the pod: 1. In Vitro Antiviral Activity and Projection of Optimized Dosing Design of Hydroxychloroquine for the Treatment of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) (https://www.ncbi.nlm.nih.gov/pubmed/32150618)2. Engineering a 3D functional human peripheral nerve in vitro using the Nerve-on-a-Chip platform. https://www.nature.com/articles/s41598-019-45407-5 3. Laboratory Animal Welfare Meets Human Welfare: A Cross-Sectional Study of Profe...
2020-04-1604 min
Toxicologic Pathology & Veterinary ScienceThe Toxicologic Pathologist's Role in the 3RsIn this podcast, Editorial Board member Elizabeth McInnes invites author Renee Hukkanen to discuss the article "The Toxicologic Pathologist's Role in the 3Rs" featured in the Vol 47, Issue 7, 2019 of Toxicologic Pathology. Click here to read the article
2020-04-0317 min
3 Minute 3Rs3 Minute 3Rs March 2020The March 2020 episode of 3 Minute 3Rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban)The papers:1. https://pubmed.ncbi.nlm.nih.gov/320766332. https://www.sciencedirect.com/science/article/pii/S02732300203005073. https://www.nature.com/articles/s41598-020-59012-4Transcript[NC3Rs] Nanomedicines have many potential advantages, including their ability to home to specific diseased tissues and exert a localised effect. Due to their relatively large size, the biodistribution of nanomedicines does not follow the same rules as small molecules...
2020-03-2003 min
Mis trucos para educarCAPÍTULO 17: Las 3RsHola a todos y bienvenidos a un nuevo capítulo del PODCAST de Mis Trucos Para Educar.En este episodio, os hablo de la importancia de las 3Rs, reducir, reciclar y reutilizar, para reparar y cuidar nuestro planeta. Al final del capítulo, podrás encontrar una idea muy bonita con la que enseñar a reciclar a los más pequeños.Recuerda que cada jueves volverás a encontrar un nuevo capítulo en esta web, donde podrás escucharlo online o descargarlo para más adelante. Además, también lo tendrás disponible en las siguientes p...
2020-03-0613 min
3 Minute 3Rs3 Minute 3Rs February 2020The February 2020 episode of 3 Minute 3Rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).1. https://www.sciencedirect.com/science/article/pii/S00928674193132362. https://www.sciencedirect.com/science/article/pii/S01650270193030973. https://www.ncbi.nlm.nih.gov/pubmed/31852563[NC3Rs] If you are bitten by a snake today, your anti venom treatment would be largely similar to if you were bitten 100 years ago.Anti venom is created by isolating antibodies from a large animal, typically a horse, after the animal has...
2020-02-2403 min
3 Minute 3Rs3 Minute 3rs January 2020The January 2020 episode of 3 Minute 3Rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).Papers:1. https://doi.org/10.15252/embr.2019497092. https://www.nature.com/articles/s41598-019-56860-73. https://doi.org/10.1177/0023677219879455[NC3Rs] Academic scientists produce many of the clever ideas which end up as medicines. However, the results in the papers reporting the work are almost always positive, and null or negative results rarely get a look in – a phenomenon known as publication bias. This is problematic as the ne...
2020-01-1604 min
Paseo Podcast: Sharing Puerto Rican StoriesBONUS: Puerto Rican Agenda's 3Rs Campaign for Earthquake Relief Efforts (Press Conference)This bonus episode is a recording of a Puerto Rican Agenda press conference Friday, January 10, in Chicago. We also have a video of the press conference on our Facebook page, if you would like to see that version instead.
The press conference was to announce the relaunch of the successful 3Rs Campaign the PR Agenda used to raise money and send supplies to the hardest hit areas of Puerto Rico after Hurricane Maria. In fact, this campaign made Chicago the first in the U.S. to send a cargo plane with supplies to la isla after Maria. N...
2020-01-1241 min
3 Minute 3Rs3 Minute 3Rs December 2019The December episode of 3 Minute 3Rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).Papers: 1. Cage aggression in group-housed laboratory male mice: an international data crowdsourcing project. https://www.nature.com/articles/s41598-019-51674-z2. Towards animal-free neurotoxicity screening: Applicability of hiPSC-derived neuronal models for in vitro seizure liability assessmenthttps://www.altex.org/index.php/altex/article/view/13213. Optimal solid state neurons https://www.nature.com/articles/s41467-019-13177-3[NC3Rs] Mice are social creatures. It's why...
2019-12-1903 min
The Mercast | Plastic Free Mermaid talks Change MakingPlastic Redesign: It's time for a new 3Rs with Pete Myers, PhDThe single most important thing for us to do about the general chemical and plastic issue is to redesign plastic. - Pete Myers Join me in this episode as we unravel the chemicals that are in plastics with Dr. Pete Myers. He is the founder and Chief Scientist of Environmental Health Sciences. In this conversation, he summarizes the presentation he did at the UNWRAPPED Conference. He talks about the challenges in dealing with the rate of global plastic productions. He also talks about redesigning plastics and the new 3Rs. Lastly, Dr. Pete shares how we c...
2019-11-2518 min
3 Minute 3Rs3 Minute 3Rs November 2019The November episode of 3 Minute 3rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).Papers:1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6662399/2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371849/3. https://pubs.rsc.org/en/content/articlelanding/2019/LC/C9LC00160C [NA3RsC] Evaluation of developmental and reproductive toxicity is a key aspect of drug development. Each year, 5-7% of newborns worldwide are born with serious birth defects, a number correlated to increased use of pharmaceutical drugs...
2019-11-2104 min
Legal Drugs Podcast9. Refining, Reducing and Replacing Animals in Research Initiatives of the North American 3Rs Collaborative with Alice White McVeyLegal Drugs Podcast, a production of Legal Drugs Agency is proud to highlight and endorse the work of the North American 3Rs Collaborative (NA3RsC) on the Legal Drugs Podcast. Host, Angela Stoyanovitch, partners up with former colleague, Alice White McVey, a guru on small animal research models with a passion for responsible 3Rs implementation. The 3Rs in research include: Refinement (methods which minimize pain and distress and/or improve welfare of the animals required) Reduction (methods which minimize the numbers of animals used) Replacement (methods which avoid, or replace, the use of animals) Alice explains that animals...
2019-11-0400 min
3 Minute 3Rs3 Minute 3Rs October 2019The October episode of 3 Minute 3rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban).Papers1. Modelling human hepato-biliary-pancreatic organogenesis from the foregut–midgut boundaryhttps://www.nature.com/articles/s41586-019-1598-02. Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms https://www.nature.com/articles/s41598-019-50920-83. Analysis of vertebrate vision in a 384-well imaging system. Thorn RJ. Sci Rep. https://www.ncbi.nlm.nih.gov/pubmed/31562366Transcript[LA] Organoids are three dimensional st...
2019-10-1704 min
Métodos para reemplazar la experimentación animalIntroducción al principio de las 3RsMaría Pilar Vinardell, catedrática de Fisiologia, Universitat de Barcelona.
Desde que en 1959 Russell y Burch postularon en el libro “The principles of Humane Experimental Tecnique” el principio de las 3Rs (Reemplazar, reducir y refinar) de la experimentación animal, se ha incorporado en las leyes nacionales e internacionales que regulan el uso de animales en procedimientos científicos.
2019-10-0235 min
3 Minute 3Rs3 Minute 3Rs September 2019The September episode of 3 Minute 3rs, brought to you by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban)Papers behind the pod:1. http://jpet.aspetjournals.org/content/371/1/15.long2. https://www.sciencedirect.com/science/article/pii/S001216061830006X3. https://www.nature.com/articles/s41467-019-11259-w[NC3Rs] Accurate measurements of drug and metabolite concentrations in the blood are vital to estimate exposure to the target in humans and animals during drug development. Zebrafish larvae are increasingly used for pharmacological research, but measurements of...
2019-09-1904 min
3 Minute 3Rs3 Minute 3Rs August 2019The August episode of 3-Minute 3Rs.Papers:1. https://advances.sciencemag.org/content/5/7/eaaw40992. https://www.ncbi.nlm.nih.gov/pubmed/310260403. https://www.ncbi.nlm.nih.gov/pubmed/31343844[LA] Nociception is the sense that tells animals they are encountering a potentially damaging stimulus. In people, we call it pain. Most pain research is done with rodents, but nociception is pretty well conserved beyond mammals. That includes invertebrates, such as the fruit fly. Some work has been done in larval drosophila, but larvae exist in a transient state; any quote unquote pain is therefore transient too. To study longer lasting changes in...
2019-08-1504 min
3 Minute 3Rs3 Minute 3Rs July 2019The July episode of 3-Minute 3Rs from the North American 3Rs Collaborative (www.na3rsc.org), the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)Papers; 1. https://advances.sciencemag.org/content/5/5/eaav65282. https://www.nature.com/articles/s41598-019-44705-23. https://www.nature.com/articles/s41598-019-45464-w[NC3Rs] Colorectal cancer is particularly hard to treat, and only 11% of patients whose tumours have metastasised survive five years after diagnosis. Mouse models that incorporate patient tissue are widely used in drug discovery, but they’re both expensive and time consuming, and often do...
2019-07-1804 min
3 Minute 3Rs3 Minute 3Rs June 2019The June episode of 3-Minute 3Rs from the North American 3Rs Collaborative (www.na3rsc.org), the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)Papers; 1. https://bit.ly/2UH4hZt2. https://go.nature.com/2W5CIea3. https://bit.ly/2Fi4td4[LA] On the road to the clinic, immunocompromised mice transplanted with patient-derived xenografts have been a frequent step for testing a drug’s efficacy and safety. However, there may soon be fisher options afoot that might help replace some of those mice. Writing in the June issue of the journal Cell, David La...
2019-06-2004 min
3 Minute 3Rs3 Minute 3Rs May 2019The May episode of 3-Minute 3Rs from the North American 3Rs Collaborative (www.na3rsc.org), the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)Papers:1. https://bit.ly/2vYuUPR2. https://go.nature.com/2w2tzre3. https://go.nature.com/2VEjEHz[NA3RC] Early mortality of pups in breeding colonies can reach upwards of 50% or higher depending upon the strain. While high pup mortality is often considered "normal", it instead may be indicative of an underestimated animal welfare issue that requires attention. Leidinger and colleagues investigate the influence of environmental enrichment as one way...
2019-05-1604 min
3 Minute 3Rs3 Minute 3Rs April 2019This is the April episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org), the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)Source papers:1. Characterizing Sources of Variability in Zebrafish Embryo Screening Protocols. https://bit.ly/2VP8njP2. Effective chair training methods for neuroscience research involving rhesus macaques. https://bit.ly/2V7NOSv3. AutonoMouse. https://bit.ly/2VP8tbb. Video: https://www.youtube.com/watch?v=6d7ctvj953M[NA3RsC] The zebrafish embryo, considered to experience little or no pain or distress, has been investigated...
2019-04-1804 min
3 Minute 3Rs3 Minute 3Rs March 2019This is the March episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org, the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)The papers behind the pod:1. Progressive Motor Neuron Pathology and the Role of Astrocytes in a Human Stem Cell Model of VCP-Related ALS. https://bit.ly/2uiF64X2. A critical evaluation of TRPA1-mediated locomotor behavior in zebrafish as a screening tool for novel anti-nociceptive drug discovery. https://go.nature.com/2Cu7u8C3. No-touch measurements of vital signs in small conscious animals. https...
2019-03-2104 min
3 Minute 3Rs3 Minute 3Rs February 2019This is the February episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org, the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)The papers behind the pod:1. 3D-printed Wash Station with Integrated Anesthesia Delivery Manifold for High-throughput Depilation of Laboratory Mice. https://bit.ly/2IpGrAP2. DeepSqueak: a deep learning-based system for detection and analysis of ultrasonic vocalizations .https://go.nature.com/2tupSJK3. Super-Mendelian inheritance mediated by CRISPR–Cas9 in the female mouse germline. https://go.nature.com/2RgnoYw[NA3RsC] Depilation (hair removal) is needed for ma...
2019-02-2103 min
3 Minute 3Rs3 Minute 3Rs January 2019This is the January episode of 3-Minute 3Rs, brought to you by the North American 3Rs Collaborative (www.na3rsc.org, the NC3Rs (www.nc3rs.org.uk), and Lab Animal (www.nature.com/laban)The papers behind the pod:1. An Atypical Parvovirus Drives Chronic Tubulointerstitial Nephropathy and Kidney Fibrosis https://bit.ly/2FA6dPA2. Remote Welfare Monitoring of Rodents Using Thermal Imaging https://bit.ly/2suGlgm3. No experimental evidence of stress-induced hyperthermia in zebrafish (Danio rerio)https://bit.ly/2Db8m2P[NA3RsC] Chronic kidney disease results from fibrosis of the kidney parenchyma due to a variety...
2019-01-1704 min
Shore Elim Church3Rs for 2019In the first message for 2019 Pastor Sef shares with us 3Rs to guide us through the year. RevelationResolution andResislienceListen in as he shares these 3Rs from the life of Simon Peter:
2019-01-1328 min
3 Minute 3Rs3 Minute 3Rs December 2018It's the December episode of 3-Minute 3Rs, brought to you by , the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org) and Lab Animal (www.nature.com/laban)The papers behind the pod:1. Optimizing the design of population-based patient-derived tumor xenograft studies to better predict clinical response https://bit.ly/2GwGJ7N2. An In Vitro Model of Hematotoxicity https://bit.ly/2Ezgaw33. Found In Translation https://go.nature.com/2UVJ0g6[NC3Rs] Every cancerous tumor is different and treating two tumors with the same drug may result in two very different outcomes...
2018-12-2004 min
3 Minute 3Rs3 Minute 3Rs November 2018It's the November episode of 3-Minute 3Rs, brought to you by Lab Animal (www.nature.com/laban), the NC3Rs (www.nc3rs.org.uk) & the North American 3Rs Collaborative (www.na3rsc.org)The papers behind the pod:1. Sixteen diverse laboratory mouse reference genomes define strain-specific haplotypes and novel functional loci. https://www.nature.com/articles/s41588-018-0223-82. Effects of Cage Enrichment on Behavior, Welfare and Outcome Variability in Female Mice https://www.frontiersin.org/articles/10.3389/fnbeh.2018.00232/full3. Effects of Music Enrichment on Individually Housed Male New Zealand White Rabbits. https://doi.org/10.30802/AALAS-JAALAS-17-000153Transcript:[NC3...
2018-11-1504 min
3 Minute 3Rs3-minute 3Rs October 2018Welcome to the October episode of 3-Minute 3Rs, brought to you by Lab Animal (www.nature.com/laban, the NC3Rs (www.nc3rs.org.uk) & the North American 3Rs Collaborative (www.na3rsc.org)Here are the papers behind the pod:1. Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids. https://www.cell.com/cell/fulltext/S0092-8674(18)30903-62. Mymou: A low-cost, wireless touchscreen system for automated training of nonhuman primates. https://link.springer.com/article/10.3758%2Fs13428-018-1109-53. Practical rat tickling: Determining an efficient and effective dosage of heterospecific play. https://www...
2018-10-1804 min
3 Minute 3Rs3-Minute 3Rs September 2018Welcome to the September episode of 3-Minute 3Rs, brought to you by Lab Animal (www.nature.com/laban), the NC3Rs (www.nc3rs.org.uk) & the North American 3Rs Collaborative (www.na3rsc.org)Here are the papers behind the pod:1. Assessing animal affect: an automated and self-initiated judgment bias task based on natural investigative behaviour. https://doi.org/10.1038/s41598-018-30571-x2. Comparison of serial blood collection by facial vein and retrobulbar methods in C57BL/6 mice. https://doi.org/10.30802/AALAS-JAALAS-17-0001343. Can grimace scales estimate the pain status in horses and mice? A statistical approach to identify...
2018-09-2004 min
3 Minute 3Rs3-Minute 3Rs August 2018You're listening to the August edition of 3 Minute 3Rs, brought to you this month by the NC3Rs (www.nc3rs.org.uk), the North American 3Rs Collaborative (www.na3rsc.org), and Lab Animal (www.nature.com/laban) This month, we'll be talking about non-primate alternatives to study polio, aging & frailty indexes, and training rats with out water restriction.The papers behind the pod:* Replacement of primary monkey kidney cells by L20B cell line in the test for effective inactivation of inactivated poliovirus vaccine:https://www.sciencedirect.com/science/article/pii/S0166093417306006* Senolytics improve physical function and increase...
2018-08-1604 min
3 Minute 3Rs3-Minute 3Rs July 2018You're listening to the July edition of 3 Minute 3Rs, brought to you this month by the North American 3Rs Collaborative (www.na3rsc.org), Lab Animal (www.nature.com/laban) and the NC3Rs (www.nc3rs.org.uk). This month, we'll be talking about refining mouse anesthesia, replacing mice with zebrafish for studying human hematopoetic stem cells, and validating genome-edited mouse lines.The papers behind the pod:* Effects of Ketamine Compared with Urethane Anesthesia on Vestibular Sensory Evoked Potentials and Systemic Physiology in Mice: http://www.ingentaconnect.com/content/aalas/jaalas/2018/00000057/00000003/art00007;jsessionid=2je0i010idu59.x-ic-live-03* A method...
2018-07-1904 min
3 Minute 3Rs3-Minute 3Rs June 2018You're listening to the June edition of 3 Minute 3Rs, brought to you this month by Lab Animal (www.nature.com/laban), the NC3Rs (www.nc3rs.org.uk) and the North American 3Rs Collaborative (www.na3rsc.org). We're highlighting Drosophila as a microbiome model, organotypic brain culture slices for Alzheimer's research, and anesthesia for African clawed frogs.The papers behind the pod:* The Drosophila model for microbiome research: https://www.nature.com/articles/s41684-018-0065-0* Preparation of organotypic brain slice cultures for the study of Alzheimer’s disease: https://f1000research.com/articles/7-592/v1* Comparison of...
2018-06-2104 min
3 Minute 3Rs3-minute 3Rs May 2018Welcome to 3-minute 3Rs for May 2018! in this podcast, we're discussing enrichment, anesthesia for cephalopods, and reproducibility across labs.The papers behind the pod:Laboratory mouse housing conditions can be improved using common environmental enrichment without compromising data: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2005019In Vivo Recording of Neural and Behavioral Correlates of Anesthesia Induction, Reversal, and Euthanasia in Cephalopod Molluscs: https://www.frontiersin.org/articles/10.3389/fphys.2018.00109/fullReproducibility of preclinical animal research improves with heterogeneity of study samples: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2003693And be sure to visit www.nature.com/laban and...
2018-05-1704 min
3 Minute 3Rs3-minute 3Rs April 2018Welcome to 3-minute 3Rs! In this podcast, we're discussing 'dirty' mice, automating pain assessments, and refining endpoints with telemetry data.The papers behind the pod:News Feature: What happens when lab animals go wild: http://www.pnas.org/content/115/13/3196A deep neural network to assess spontaneous pain from mouse facial expressions: http://journals.sagepub.com/doi/10.1177/1744806918763658Using Telemetry Data to Refine Endpoints for New Zealand White Rabbits Challenged with Bacillus anthracis https://www.ncbi.nlm.nih.gov/pubmed/29256375 Hosted on Acast. See acast.com/privacy for more information.
2018-04-1904 min
3 Minute 3Rs3-minute 3Rs March 2018In this month's podcast, we're highlighting the papers recognized by the NC3Rs International Prize, with topics including in silico modeling to predict cardiac drug toxicity, a novel experimental design to reduce variability, and affimers as an alternative to antibodiesRead more about the Prize and the research here: https://www.nc3rs.org.uk/3rsprizewinners Hosted on Acast. See acast.com/privacy for more information.
2018-03-2204 min
3 Minute 3Rs3-minute 3Rs February 2018In this month's 3-minute 3R's, we're talking temperature, environmental enrichment to improve stress responses in zebrafish, and better ways to handle mice.The papers behind the pod:Mice Housed at Elevated Vivarium Temperatures Display Enhanced T-cell Response and Survival to Francisella tularensis: http://www.ingentaconnect.com/content/aalas/cm/2017/00000067/00000006/art00004Heat or Insulation: Behavioral Titration of Mouse Preference for Warmth or Access to a Nest: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0032799International survey on the use and welfare of zebrafish Danio rerio in research: http://onlinelibrary.wiley.com/doi/10.1111/jfb.13278/abstractEnvironmental enrichment modulates the response to chronic...
2018-02-2204 min
3 Minute 3Rs3-minute 3Rs January 2018In this month's 3-minute 3Rs: A review on using zebrafish embryos to help improve nano-toxicology studies (http://www.tandfonline.com/doi/abs/10.1080/10408444.2017.1404965?journalCode=itxc20)Turning plain language summaries of animal experiments into analyzable data (http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2003217)And, a new protocol for making knockout mice in a single generation (https://www.nature.com/articles/nprot.2017.110)Find out more at Lab Animal (www.nature.com/laban) or the NC3Rs (NC3Rs.org.uk). Hosted on Acast. See acast.com/privacy for more information.
2018-01-1804 min
3 Minute 3Rs3-Minute-3Rs December 20173 minute 3Rs, brought to you by Lab Animal at Nature Research and the NC3Rs in London, is a quick monthly podcast summarizing the latest research and news in 3Rs Science and Technology. In this month's podcast:A "transparent ribcage" for imaging tumor dynamics with cellular resolution in murine lungs (https://www.nature.com/articles/nmeth.4511)The future of tissue chips for improving drug discovery (https://doi.org/10.1177/1535370217715441)Robotic zebrafish for probing social behavioral (http://iopscience.iop.org/article/10.1088/1748-3190/aa8f6a) Hosted on Acast. See acast.com/privacy for more information.
2017-12-1405 min
Knowing AnimalsEpisode 53: the 3Rs with Charlotte BlattnerThis episode of Knowing Animals comes from Switzerland. I speak to Dr. Charlotte Blattner, currently at Queens University, Canada, about her book chapter 'Rethinking the 3Rs: From Whitewashing to Rights, which appears in the 2018 book 'Animal Experimentation: Working Towards a Paradigm Change', published by Brill. This episode is supported by the Australasian Animal Studies Association - https://www.facebook.com/AASA-Australasian-Animal-Studies-Association-480316142116752/. The new Animal Studies book 'After Coetzee: An Anthology of Animal Fictions' - https://www.facebook.com/aftercoetzee/. And Kibbl, the phone app that helps you adopt animals from pounds - https://ww...
2017-11-2029 min
3 Minute 3Rs3 Minute 3Rs November3 minute 3Rs, brought to you by Lab Animal at Nature Research and the NC3Rs in London, is a quick monthly podcast summarizing the latest research and news in 3Rs Science and Technology. This month, we look at rodent big brother for 24/7 remote monitoring of rodent behavior, the alliance of genome resources, and the experimental design assistant for improving the rigor and reproducibility of animal research. Hosted on Acast. See acast.com/privacy for more information.
2017-11-1604 min